Cooling of equine semen at 16°C for 36 hours with addition of different glutathione concentrations

Detalhes bibliográficos
Autor(a) principal: Oliveira, Rodrigo Arruda de
Data de Publicação: 2015
Outros Autores: Viu, Marco Antônio de Oliveira, Gambarini, Maria Lúcia
Tipo de documento: Artigo
Idioma: eng
por
Título da fonte: Semina. Ciências Agrárias (Online)
Texto Completo: https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/19182
Resumo: Handling equine semen during the refrigeration process reduces sperm viability, and consequently causes membrane lipid peroxidation, among other challenges. The present study aimed to evaluate the in vitro effects of glutathione (control, 1. 0, 1. 5, and 2. 5 mM) on equine semen in a refrigeration protocol of 16ºC for 36 hours. The following variables were evaluated after 0, 12, 24, and 36 hours refrigeration: total sperm motility, vigor, viability, and plasma and acrosomal membrane integrity. Motility was higher with 2. 5mM of glutathione (57. 8 ± 7. 3) after 12 hours of refrigeration compared to the control (53. 2 ± 8. 3) (P < 0. 05). After 36 hours of refrigeration, motility was higher with 1. 5 mM (43. 4 ± 12. 7) and 2. 5mM glutathione (45. 5 ± 6. 2), than it was with 1mM glutathione (38. 2 ± 9) and the control (35. 5 ± 18. 4) (P < 0. 05), respectively. Vigor was highest with 1. 5mM glutathione (3. 7 ± 0. 3) after 36 hours compared to the control (3. 2 ± 1. 1), (P < 0. 05). Viability differed between control and 1mM treatments (79. 5 ± 1. 8) only after 24 hours (75. 5 ± 9. 7) (P < 0. 05). Throughout the investigation, no significant differences were noted in plasma and acrosomal membrane integrity (P > 0. 05). The 1. 5 and 2. 5mM glutathione levels were more efficient in protecting sperm cells and yielded higher total motility values after 36 hours of refrigeration.
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spelling Cooling of equine semen at 16°C for 36 hours with addition of different glutathione concentrationsRefrigeração do sêmen equino a 16ºC por 36h com adição de glutationa em diferentes concentraçõesAntioxidantSpermStallionCooled semen.AntioxidanteEspermatozóideGaranhãoSêmen refrigerado.Handling equine semen during the refrigeration process reduces sperm viability, and consequently causes membrane lipid peroxidation, among other challenges. The present study aimed to evaluate the in vitro effects of glutathione (control, 1. 0, 1. 5, and 2. 5 mM) on equine semen in a refrigeration protocol of 16ºC for 36 hours. The following variables were evaluated after 0, 12, 24, and 36 hours refrigeration: total sperm motility, vigor, viability, and plasma and acrosomal membrane integrity. Motility was higher with 2. 5mM of glutathione (57. 8 ± 7. 3) after 12 hours of refrigeration compared to the control (53. 2 ± 8. 3) (P < 0. 05). After 36 hours of refrigeration, motility was higher with 1. 5 mM (43. 4 ± 12. 7) and 2. 5mM glutathione (45. 5 ± 6. 2), than it was with 1mM glutathione (38. 2 ± 9) and the control (35. 5 ± 18. 4) (P < 0. 05), respectively. Vigor was highest with 1. 5mM glutathione (3. 7 ± 0. 3) after 36 hours compared to the control (3. 2 ± 1. 1), (P < 0. 05). Viability differed between control and 1mM treatments (79. 5 ± 1. 8) only after 24 hours (75. 5 ± 9. 7) (P < 0. 05). Throughout the investigation, no significant differences were noted in plasma and acrosomal membrane integrity (P > 0. 05). The 1. 5 and 2. 5mM glutathione levels were more efficient in protecting sperm cells and yielded higher total motility values after 36 hours of refrigeration.A manipulação do sêmen equino durante o processo de refrigeração reduz a viabilidade espermática em consequência, entre outros problemas, da peroxidação de lipídios da membrana. Objetivou-se avaliar o efeito in vitro da adição de glutationa (controle; 1,0; 1,5 e 2,5mM) em protocolo de refrigeração a 16ºC por 36h. As variáveis avaliadas foram motilidade total, vigor, viabilidade espermática e integridade das membranas plasmática e acrossomal em quatro momentos diferentes de refrigeração (0, 12, 24 e 36h). A motilidade foi superior com 2,5mM de glutationa (57,8±7,3) com 12h de refrigeração quando comparado ao controle (53,2±8,3), P<0,05. Com 36h de refrigeração a motilidade foi superior com 1,5mM (43,4±12,7) e 2,5mM (45,5±6,2) quando comparado com 1mM (38,2±9) e ao controle (35,5±18,4), P<0,05. Com relação ao vigor houve superioridade com 1,5mM de glutationa (3,7±0,3), na avaliação de 36h, em comparação ao controle (3,2±1,1), P<0,05. Para viabilidade só houve diferença em relação ao controle na avaliação de 24h (75,5±9,7) quando comparado ao tratamento 1mM (79,5±1,8), P<0,05. Não houve diferença em relação ao controle para as avaliações de integridade de membrana plasmática e acrosomal, em todos os momentos de avaliação, P>0,05. As concentrações de 1,5 e 2,5MM de glutationa foram mais eficientes para proteção da célula espermática com valores superiores de motilidade total na avaliação de 36h.UEL2015-12-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionPesquisaapplication/pdfapplication/vnd.openxmlformats-officedocument.wordprocessingml.documentimage/jpegimage/pjpeghttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/1918210.5433/1679-0359.2015v36n6p3699Semina: Ciências Agrárias; Vol. 36 No. 6 (2015); 3699-3704Semina: Ciências Agrárias; v. 36 n. 6 (2015); 3699-37041679-03591676-546Xreponame:Semina. Ciências Agrárias (Online)instname:Universidade Estadual de Londrina (UEL)instacron:UELengporhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/19182/17452https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/19182/34609https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/19182/34610https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/19182/36887Oliveira, Rodrigo Arruda deViu, Marco Antônio de OliveiraGambarini, Maria Lúciainfo:eu-repo/semantics/openAccess2015-12-09T15:01:19Zoai:ojs.pkp.sfu.ca:article/19182Revistahttp://www.uel.br/revistas/uel/index.php/semagrariasPUBhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/oaisemina.agrarias@uel.br1679-03591676-546Xopendoar:2015-12-09T15:01:19Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)false
dc.title.none.fl_str_mv Cooling of equine semen at 16°C for 36 hours with addition of different glutathione concentrations
Refrigeração do sêmen equino a 16ºC por 36h com adição de glutationa em diferentes concentrações
title Cooling of equine semen at 16°C for 36 hours with addition of different glutathione concentrations
spellingShingle Cooling of equine semen at 16°C for 36 hours with addition of different glutathione concentrations
Oliveira, Rodrigo Arruda de
Antioxidant
Sperm
Stallion
Cooled semen.
Antioxidante
Espermatozóide
Garanhão
Sêmen refrigerado.
title_short Cooling of equine semen at 16°C for 36 hours with addition of different glutathione concentrations
title_full Cooling of equine semen at 16°C for 36 hours with addition of different glutathione concentrations
title_fullStr Cooling of equine semen at 16°C for 36 hours with addition of different glutathione concentrations
title_full_unstemmed Cooling of equine semen at 16°C for 36 hours with addition of different glutathione concentrations
title_sort Cooling of equine semen at 16°C for 36 hours with addition of different glutathione concentrations
author Oliveira, Rodrigo Arruda de
author_facet Oliveira, Rodrigo Arruda de
Viu, Marco Antônio de Oliveira
Gambarini, Maria Lúcia
author_role author
author2 Viu, Marco Antônio de Oliveira
Gambarini, Maria Lúcia
author2_role author
author
dc.contributor.author.fl_str_mv Oliveira, Rodrigo Arruda de
Viu, Marco Antônio de Oliveira
Gambarini, Maria Lúcia
dc.subject.por.fl_str_mv Antioxidant
Sperm
Stallion
Cooled semen.
Antioxidante
Espermatozóide
Garanhão
Sêmen refrigerado.
topic Antioxidant
Sperm
Stallion
Cooled semen.
Antioxidante
Espermatozóide
Garanhão
Sêmen refrigerado.
description Handling equine semen during the refrigeration process reduces sperm viability, and consequently causes membrane lipid peroxidation, among other challenges. The present study aimed to evaluate the in vitro effects of glutathione (control, 1. 0, 1. 5, and 2. 5 mM) on equine semen in a refrigeration protocol of 16ºC for 36 hours. The following variables were evaluated after 0, 12, 24, and 36 hours refrigeration: total sperm motility, vigor, viability, and plasma and acrosomal membrane integrity. Motility was higher with 2. 5mM of glutathione (57. 8 ± 7. 3) after 12 hours of refrigeration compared to the control (53. 2 ± 8. 3) (P < 0. 05). After 36 hours of refrigeration, motility was higher with 1. 5 mM (43. 4 ± 12. 7) and 2. 5mM glutathione (45. 5 ± 6. 2), than it was with 1mM glutathione (38. 2 ± 9) and the control (35. 5 ± 18. 4) (P < 0. 05), respectively. Vigor was highest with 1. 5mM glutathione (3. 7 ± 0. 3) after 36 hours compared to the control (3. 2 ± 1. 1), (P < 0. 05). Viability differed between control and 1mM treatments (79. 5 ± 1. 8) only after 24 hours (75. 5 ± 9. 7) (P < 0. 05). Throughout the investigation, no significant differences were noted in plasma and acrosomal membrane integrity (P > 0. 05). The 1. 5 and 2. 5mM glutathione levels were more efficient in protecting sperm cells and yielded higher total motility values after 36 hours of refrigeration.
publishDate 2015
dc.date.none.fl_str_mv 2015-12-09
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Pesquisa
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/19182
10.5433/1679-0359.2015v36n6p3699
url https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/19182
identifier_str_mv 10.5433/1679-0359.2015v36n6p3699
dc.language.iso.fl_str_mv eng
por
language eng
por
dc.relation.none.fl_str_mv https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/19182/17452
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/19182/34609
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/19182/34610
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/19182/36887
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/vnd.openxmlformats-officedocument.wordprocessingml.document
image/jpeg
image/pjpeg
dc.publisher.none.fl_str_mv UEL
publisher.none.fl_str_mv UEL
dc.source.none.fl_str_mv Semina: Ciências Agrárias; Vol. 36 No. 6 (2015); 3699-3704
Semina: Ciências Agrárias; v. 36 n. 6 (2015); 3699-3704
1679-0359
1676-546X
reponame:Semina. Ciências Agrárias (Online)
instname:Universidade Estadual de Londrina (UEL)
instacron:UEL
instname_str Universidade Estadual de Londrina (UEL)
instacron_str UEL
institution UEL
reponame_str Semina. Ciências Agrárias (Online)
collection Semina. Ciências Agrárias (Online)
repository.name.fl_str_mv Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)
repository.mail.fl_str_mv semina.agrarias@uel.br
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