Salivary metabolomic profile in adolescents with juvenile systemic lupus erythematosus
Main Author: | |
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Publication Date: | 2022 |
Other Authors: | , , , , , , |
Format: | Article |
Language: | eng |
Source: | Brazilian Oral Research |
Download full: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242022000100309 |
Summary: | Abstract The aim of this study was to characterize the salivary metabolomic profile in adolescents with juvenile systemic lupus erythematosus (jSLE). A total of 24 adolescents with jSLE (15.92 ± 2.06 years) and 12 systemically healthy controls (15.25 ± 2.7 years) were included in the study. Participants underwent rheumatologic testing and periodontal examination, with the recording of plaque index (PI), probing depth (PD), clinical attachment level (CAL), and bleeding on probing index (BPI). Unstimulated whole saliva was collected from both groups and stored at -80 ºC. The salivary proton nuclear magnetic resonance (1H-NMR) spectra were acquired in a spectrometer operating at 500 MHz. Partial least squared discriminant analysis (PLS-DA) and orthogonal PLS-DA (O-PLS-DA) were used for statistical analysis. Mean CAL and PI were significantly increased in the group with jSLE (p < 0.01). Patients with jSLE presented a significantly different salivary metabolic profile (accuracy = 0.54; R2 = 0.86; Q2 = -0.293), significantly higher salivary levels of N-acetyl sugars, and significantly reduced levels of phenylalanine, glycine, taurine, hydroxybutyrate, and valerate compared with healthy controls (p < 0.05). It is suggested that the salivary metabolomic profile analyzed by 1H NMR in patients with jSLE presents a different fingerprint that the systemically healthy subjects. Integrating the variation of metabolites with the identification of the metabolic pathways involved seems to provide a better understanding of the influence of systemic disease on salivary metabolites. |
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Salivary metabolomic profile in adolescents with juvenile systemic lupus erythematosusGingivitisMetabolomicsSalivaAdolescentMagnetic Resonance SpectroscopyAbstract The aim of this study was to characterize the salivary metabolomic profile in adolescents with juvenile systemic lupus erythematosus (jSLE). A total of 24 adolescents with jSLE (15.92 ± 2.06 years) and 12 systemically healthy controls (15.25 ± 2.7 years) were included in the study. Participants underwent rheumatologic testing and periodontal examination, with the recording of plaque index (PI), probing depth (PD), clinical attachment level (CAL), and bleeding on probing index (BPI). Unstimulated whole saliva was collected from both groups and stored at -80 ºC. The salivary proton nuclear magnetic resonance (1H-NMR) spectra were acquired in a spectrometer operating at 500 MHz. Partial least squared discriminant analysis (PLS-DA) and orthogonal PLS-DA (O-PLS-DA) were used for statistical analysis. Mean CAL and PI were significantly increased in the group with jSLE (p < 0.01). Patients with jSLE presented a significantly different salivary metabolic profile (accuracy = 0.54; R2 = 0.86; Q2 = -0.293), significantly higher salivary levels of N-acetyl sugars, and significantly reduced levels of phenylalanine, glycine, taurine, hydroxybutyrate, and valerate compared with healthy controls (p < 0.05). It is suggested that the salivary metabolomic profile analyzed by 1H NMR in patients with jSLE presents a different fingerprint that the systemically healthy subjects. Integrating the variation of metabolites with the identification of the metabolic pathways involved seems to provide a better understanding of the influence of systemic disease on salivary metabolites.Sociedade Brasileira de Pesquisa Odontológica - SBPqO2022-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242022000100309Brazilian Oral Research v.36 2022reponame:Brazilian Oral Researchinstname:Sociedade Brasileira de Pesquisa Odontológica (SBPqO)instacron:SBPQO10.1590/1807-3107bor-2022.vol36.0128info:eu-repo/semantics/openAccessBRAGARD,Loreley Carlos AgostinhoSETE,Manuela Rubim CamaraFREITAS-FERNANDES,Liana BastosSZTAJNBOK,Flavio RobertoFIGUEREDO,Carlos MarceloVALENTE,Ana PaulaFIDALGO,Tatiana Kelly da SilvaSILVA,Fernanda de Britoeng2022-11-07T00:00:00Zoai:scielo:S1806-83242022000100309Revistahttps://www.scielo.br/j/bor/https://old.scielo.br/oai/scielo-oai.phppob@edu.usp.br||bor@sbpqo.org.br1807-31071806-8324opendoar:2022-11-07T00:00Brazilian Oral Research - Sociedade Brasileira de Pesquisa Odontológica (SBPqO)false |
dc.title.none.fl_str_mv |
Salivary metabolomic profile in adolescents with juvenile systemic lupus erythematosus |
title |
Salivary metabolomic profile in adolescents with juvenile systemic lupus erythematosus |
spellingShingle |
Salivary metabolomic profile in adolescents with juvenile systemic lupus erythematosus BRAGARD,Loreley Carlos Agostinho Gingivitis Metabolomics Saliva Adolescent Magnetic Resonance Spectroscopy |
title_short |
Salivary metabolomic profile in adolescents with juvenile systemic lupus erythematosus |
title_full |
Salivary metabolomic profile in adolescents with juvenile systemic lupus erythematosus |
title_fullStr |
Salivary metabolomic profile in adolescents with juvenile systemic lupus erythematosus |
title_full_unstemmed |
Salivary metabolomic profile in adolescents with juvenile systemic lupus erythematosus |
title_sort |
Salivary metabolomic profile in adolescents with juvenile systemic lupus erythematosus |
author |
BRAGARD,Loreley Carlos Agostinho |
author_facet |
BRAGARD,Loreley Carlos Agostinho SETE,Manuela Rubim Camara FREITAS-FERNANDES,Liana Bastos SZTAJNBOK,Flavio Roberto FIGUEREDO,Carlos Marcelo VALENTE,Ana Paula FIDALGO,Tatiana Kelly da Silva SILVA,Fernanda de Brito |
author_role |
author |
author2 |
SETE,Manuela Rubim Camara FREITAS-FERNANDES,Liana Bastos SZTAJNBOK,Flavio Roberto FIGUEREDO,Carlos Marcelo VALENTE,Ana Paula FIDALGO,Tatiana Kelly da Silva SILVA,Fernanda de Brito |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
BRAGARD,Loreley Carlos Agostinho SETE,Manuela Rubim Camara FREITAS-FERNANDES,Liana Bastos SZTAJNBOK,Flavio Roberto FIGUEREDO,Carlos Marcelo VALENTE,Ana Paula FIDALGO,Tatiana Kelly da Silva SILVA,Fernanda de Brito |
dc.subject.por.fl_str_mv |
Gingivitis Metabolomics Saliva Adolescent Magnetic Resonance Spectroscopy |
topic |
Gingivitis Metabolomics Saliva Adolescent Magnetic Resonance Spectroscopy |
description |
Abstract The aim of this study was to characterize the salivary metabolomic profile in adolescents with juvenile systemic lupus erythematosus (jSLE). A total of 24 adolescents with jSLE (15.92 ± 2.06 years) and 12 systemically healthy controls (15.25 ± 2.7 years) were included in the study. Participants underwent rheumatologic testing and periodontal examination, with the recording of plaque index (PI), probing depth (PD), clinical attachment level (CAL), and bleeding on probing index (BPI). Unstimulated whole saliva was collected from both groups and stored at -80 ºC. The salivary proton nuclear magnetic resonance (1H-NMR) spectra were acquired in a spectrometer operating at 500 MHz. Partial least squared discriminant analysis (PLS-DA) and orthogonal PLS-DA (O-PLS-DA) were used for statistical analysis. Mean CAL and PI were significantly increased in the group with jSLE (p < 0.01). Patients with jSLE presented a significantly different salivary metabolic profile (accuracy = 0.54; R2 = 0.86; Q2 = -0.293), significantly higher salivary levels of N-acetyl sugars, and significantly reduced levels of phenylalanine, glycine, taurine, hydroxybutyrate, and valerate compared with healthy controls (p < 0.05). It is suggested that the salivary metabolomic profile analyzed by 1H NMR in patients with jSLE presents a different fingerprint that the systemically healthy subjects. Integrating the variation of metabolites with the identification of the metabolic pathways involved seems to provide a better understanding of the influence of systemic disease on salivary metabolites. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242022000100309 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242022000100309 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/1807-3107bor-2022.vol36.0128 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Pesquisa Odontológica - SBPqO |
publisher.none.fl_str_mv |
Sociedade Brasileira de Pesquisa Odontológica - SBPqO |
dc.source.none.fl_str_mv |
Brazilian Oral Research v.36 2022 reponame:Brazilian Oral Research instname:Sociedade Brasileira de Pesquisa Odontológica (SBPqO) instacron:SBPQO |
instname_str |
Sociedade Brasileira de Pesquisa Odontológica (SBPqO) |
instacron_str |
SBPQO |
institution |
SBPQO |
reponame_str |
Brazilian Oral Research |
collection |
Brazilian Oral Research |
repository.name.fl_str_mv |
Brazilian Oral Research - Sociedade Brasileira de Pesquisa Odontológica (SBPqO) |
repository.mail.fl_str_mv |
pob@edu.usp.br||bor@sbpqo.org.br |
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1750318328519327744 |