Comparison of the hemolytic activity between C. albicans and non-albicans Candida species
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Oral Research |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242013000600484 |
Resumo: | The ability to produce enzymes, such as hemolysins, is an important virulence factor for the genus Candida.The objective of this study was to compare the hemolytic activity between C. albicansand non-albicans Candida species. Fifty strains of Candida species, isolated from the oral cavity of patients infected with HIV were studied. The isolates included the following species: C. albicans, C. dubliniensis, C. glabrata, C. tropicalis, C. krusei, C. parapsilosis, C. dubliniensis, C. norvegensis, C. lusitaniae, and C. guilliermondii. Hemolysin production was evaluated on Sabouraud dextrose agar containing chloramphenicol, blood, and glucose. A loop-full of pure Candidaculture was spot-inoculated onto plates and incubated at 37ºC for 24 h in a 5% CO2 atmosphere. Hemolytic activity was defined as the formation of a translucent halo around the colonies. All C. albicansstrains that were studied produced hemolysins. Among the non-albicans Candidaspecies, 86% exhibited hemolytic activity. Only C. guilliermondiiand some C. parapsilosis isolates were negative for this enzyme. In conclusion, most non-albicans Candidaspecies had a similar ability to produce hemolysins when compared to C. albicans. |
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Comparison of the hemolytic activity between C. albicans and non-albicans Candida speciesCandidaVirulence FactorsAcquired Immunodeficiency SyndromeThe ability to produce enzymes, such as hemolysins, is an important virulence factor for the genus Candida.The objective of this study was to compare the hemolytic activity between C. albicansand non-albicans Candida species. Fifty strains of Candida species, isolated from the oral cavity of patients infected with HIV were studied. The isolates included the following species: C. albicans, C. dubliniensis, C. glabrata, C. tropicalis, C. krusei, C. parapsilosis, C. dubliniensis, C. norvegensis, C. lusitaniae, and C. guilliermondii. Hemolysin production was evaluated on Sabouraud dextrose agar containing chloramphenicol, blood, and glucose. A loop-full of pure Candidaculture was spot-inoculated onto plates and incubated at 37ºC for 24 h in a 5% CO2 atmosphere. Hemolytic activity was defined as the formation of a translucent halo around the colonies. All C. albicansstrains that were studied produced hemolysins. Among the non-albicans Candidaspecies, 86% exhibited hemolytic activity. Only C. guilliermondiiand some C. parapsilosis isolates were negative for this enzyme. In conclusion, most non-albicans Candidaspecies had a similar ability to produce hemolysins when compared to C. albicans.Sociedade Brasileira de Pesquisa Odontológica - SBPqO2013-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242013000600484Brazilian Oral Research v.27 n.6 2013reponame:Brazilian Oral Researchinstname:Sociedade Brasileira de Pesquisa Odontológica (SBPqO)instacron:SBPQO10.1590/S1806-83242013000600007info:eu-repo/semantics/openAccessRossoni,Rodnei DennisBarbosa,Junia OliveiraVilela,Simone Furgeri GodinhoJorge,Antonio Olavo CardosoJunqueira,Juliana Camposeng2014-01-09T00:00:00Zoai:scielo:S1806-83242013000600484Revistahttps://www.scielo.br/j/bor/https://old.scielo.br/oai/scielo-oai.phppob@edu.usp.br||bor@sbpqo.org.br1807-31071806-8324opendoar:2014-01-09T00:00Brazilian Oral Research - Sociedade Brasileira de Pesquisa Odontológica (SBPqO)false |
dc.title.none.fl_str_mv |
Comparison of the hemolytic activity between C. albicans and non-albicans Candida species |
title |
Comparison of the hemolytic activity between C. albicans and non-albicans Candida species |
spellingShingle |
Comparison of the hemolytic activity between C. albicans and non-albicans Candida species Rossoni,Rodnei Dennis Candida Virulence Factors Acquired Immunodeficiency Syndrome |
title_short |
Comparison of the hemolytic activity between C. albicans and non-albicans Candida species |
title_full |
Comparison of the hemolytic activity between C. albicans and non-albicans Candida species |
title_fullStr |
Comparison of the hemolytic activity between C. albicans and non-albicans Candida species |
title_full_unstemmed |
Comparison of the hemolytic activity between C. albicans and non-albicans Candida species |
title_sort |
Comparison of the hemolytic activity between C. albicans and non-albicans Candida species |
author |
Rossoni,Rodnei Dennis |
author_facet |
Rossoni,Rodnei Dennis Barbosa,Junia Oliveira Vilela,Simone Furgeri Godinho Jorge,Antonio Olavo Cardoso Junqueira,Juliana Campos |
author_role |
author |
author2 |
Barbosa,Junia Oliveira Vilela,Simone Furgeri Godinho Jorge,Antonio Olavo Cardoso Junqueira,Juliana Campos |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Rossoni,Rodnei Dennis Barbosa,Junia Oliveira Vilela,Simone Furgeri Godinho Jorge,Antonio Olavo Cardoso Junqueira,Juliana Campos |
dc.subject.por.fl_str_mv |
Candida Virulence Factors Acquired Immunodeficiency Syndrome |
topic |
Candida Virulence Factors Acquired Immunodeficiency Syndrome |
description |
The ability to produce enzymes, such as hemolysins, is an important virulence factor for the genus Candida.The objective of this study was to compare the hemolytic activity between C. albicansand non-albicans Candida species. Fifty strains of Candida species, isolated from the oral cavity of patients infected with HIV were studied. The isolates included the following species: C. albicans, C. dubliniensis, C. glabrata, C. tropicalis, C. krusei, C. parapsilosis, C. dubliniensis, C. norvegensis, C. lusitaniae, and C. guilliermondii. Hemolysin production was evaluated on Sabouraud dextrose agar containing chloramphenicol, blood, and glucose. A loop-full of pure Candidaculture was spot-inoculated onto plates and incubated at 37ºC for 24 h in a 5% CO2 atmosphere. Hemolytic activity was defined as the formation of a translucent halo around the colonies. All C. albicansstrains that were studied produced hemolysins. Among the non-albicans Candidaspecies, 86% exhibited hemolytic activity. Only C. guilliermondiiand some C. parapsilosis isolates were negative for this enzyme. In conclusion, most non-albicans Candidaspecies had a similar ability to produce hemolysins when compared to C. albicans. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-12-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242013000600484 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242013000600484 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1806-83242013000600007 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Pesquisa Odontológica - SBPqO |
publisher.none.fl_str_mv |
Sociedade Brasileira de Pesquisa Odontológica - SBPqO |
dc.source.none.fl_str_mv |
Brazilian Oral Research v.27 n.6 2013 reponame:Brazilian Oral Research instname:Sociedade Brasileira de Pesquisa Odontológica (SBPqO) instacron:SBPQO |
instname_str |
Sociedade Brasileira de Pesquisa Odontológica (SBPqO) |
instacron_str |
SBPQO |
institution |
SBPQO |
reponame_str |
Brazilian Oral Research |
collection |
Brazilian Oral Research |
repository.name.fl_str_mv |
Brazilian Oral Research - Sociedade Brasileira de Pesquisa Odontológica (SBPqO) |
repository.mail.fl_str_mv |
pob@edu.usp.br||bor@sbpqo.org.br |
_version_ |
1750318323432685568 |