Comparison of the hemolytic activity between C. albicans and non-albicans Candida species
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/S1806-83242013000600007 http://hdl.handle.net/11449/109662 |
Resumo: | The ability to produce enzymes, such as hemolysins, is an important virulence factor for the genus Candida.The objective of this study was to compare the hemolytic activity between C. albicansand non-albicans Candida species. Fifty strains of Candida species, isolated from the oral cavity of patients infected with HIV were studied. The isolates included the following species: C. albicans, C. dubliniensis, C. glabrata, C. tropicalis, C. krusei, C. parapsilosis, C. dubliniensis, C. norvegensis, C. lusitaniae, and C. guilliermondii. Hemolysin production was evaluated on Sabouraud dextrose agar containing chloramphenicol, blood, and glucose. A loop-full of pure Candidaculture was spot-inoculated onto plates and incubated at 37ºC for 24 h in a 5% CO2 atmosphere. Hemolytic activity was defined as the formation of a translucent halo around the colonies. All C. albicansstrains that were studied produced hemolysins. Among the non-albicans Candidaspecies, 86% exhibited hemolytic activity. Only C. guilliermondiiand some C. parapsilosis isolates were negative for this enzyme. In conclusion, most non-albicans Candidaspecies had a similar ability to produce hemolysins when compared to C. albicans. |
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Comparison of the hemolytic activity between C. albicans and non-albicans Candida speciesCandidaVirulence FactorsAcquired Immunodeficiency SyndromeThe ability to produce enzymes, such as hemolysins, is an important virulence factor for the genus Candida.The objective of this study was to compare the hemolytic activity between C. albicansand non-albicans Candida species. Fifty strains of Candida species, isolated from the oral cavity of patients infected with HIV were studied. The isolates included the following species: C. albicans, C. dubliniensis, C. glabrata, C. tropicalis, C. krusei, C. parapsilosis, C. dubliniensis, C. norvegensis, C. lusitaniae, and C. guilliermondii. Hemolysin production was evaluated on Sabouraud dextrose agar containing chloramphenicol, blood, and glucose. A loop-full of pure Candidaculture was spot-inoculated onto plates and incubated at 37ºC for 24 h in a 5% CO2 atmosphere. Hemolytic activity was defined as the formation of a translucent halo around the colonies. All C. albicansstrains that were studied produced hemolysins. Among the non-albicans Candidaspecies, 86% exhibited hemolytic activity. Only C. guilliermondiiand some C. parapsilosis isolates were negative for this enzyme. In conclusion, most non-albicans Candidaspecies had a similar ability to produce hemolysins when compared to C. albicans.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Universidade Estadual Paulista Institute of Science and Technology Department of Biosciences and Oral DiagnosisUniversidade Estadual Paulista Institute of Science and Technology Department of Biosciences and Oral DiagnosisFAPESP: 07/54442-3FAPESP: 12/02184-9Sociedade Brasileira de Pesquisa Odontológica (SBPqO)Universidade Estadual Paulista (Unesp)Rossoni, Rodnei DennisBarbosa, Junia OliveiraVilela, Simone Furgeri GodinhoJorge, Antonio Olavo Cardoso [UNESP]Junqueira, Juliana Campos2014-09-30T18:18:33Z2014-09-30T18:18:33Z2013-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article484-489application/pdfhttp://dx.doi.org/10.1590/S1806-83242013000600007Brazilian Oral Research. Sociedade Brasileira de Pesquisa Odontológica - SBPqO, v. 27, n. 6, p. 484-489, 2013.1806-8324http://hdl.handle.net/11449/10966210.1590/S1806-83242013000600007S1806-83242013000600484WOS:000327281300007S1806-83242013000600484.pdf166468835780429900535671536235690322020541055900SciELOreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBrazilian Oral Researchinfo:eu-repo/semantics/openAccess2023-11-25T06:16:17Zoai:repositorio.unesp.br:11449/109662Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-11-25T06:16:17Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Comparison of the hemolytic activity between C. albicans and non-albicans Candida species |
title |
Comparison of the hemolytic activity between C. albicans and non-albicans Candida species |
spellingShingle |
Comparison of the hemolytic activity between C. albicans and non-albicans Candida species Rossoni, Rodnei Dennis Candida Virulence Factors Acquired Immunodeficiency Syndrome |
title_short |
Comparison of the hemolytic activity between C. albicans and non-albicans Candida species |
title_full |
Comparison of the hemolytic activity between C. albicans and non-albicans Candida species |
title_fullStr |
Comparison of the hemolytic activity between C. albicans and non-albicans Candida species |
title_full_unstemmed |
Comparison of the hemolytic activity between C. albicans and non-albicans Candida species |
title_sort |
Comparison of the hemolytic activity between C. albicans and non-albicans Candida species |
author |
Rossoni, Rodnei Dennis |
author_facet |
Rossoni, Rodnei Dennis Barbosa, Junia Oliveira Vilela, Simone Furgeri Godinho Jorge, Antonio Olavo Cardoso [UNESP] Junqueira, Juliana Campos |
author_role |
author |
author2 |
Barbosa, Junia Oliveira Vilela, Simone Furgeri Godinho Jorge, Antonio Olavo Cardoso [UNESP] Junqueira, Juliana Campos |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Rossoni, Rodnei Dennis Barbosa, Junia Oliveira Vilela, Simone Furgeri Godinho Jorge, Antonio Olavo Cardoso [UNESP] Junqueira, Juliana Campos |
dc.subject.por.fl_str_mv |
Candida Virulence Factors Acquired Immunodeficiency Syndrome |
topic |
Candida Virulence Factors Acquired Immunodeficiency Syndrome |
description |
The ability to produce enzymes, such as hemolysins, is an important virulence factor for the genus Candida.The objective of this study was to compare the hemolytic activity between C. albicansand non-albicans Candida species. Fifty strains of Candida species, isolated from the oral cavity of patients infected with HIV were studied. The isolates included the following species: C. albicans, C. dubliniensis, C. glabrata, C. tropicalis, C. krusei, C. parapsilosis, C. dubliniensis, C. norvegensis, C. lusitaniae, and C. guilliermondii. Hemolysin production was evaluated on Sabouraud dextrose agar containing chloramphenicol, blood, and glucose. A loop-full of pure Candidaculture was spot-inoculated onto plates and incubated at 37ºC for 24 h in a 5% CO2 atmosphere. Hemolytic activity was defined as the formation of a translucent halo around the colonies. All C. albicansstrains that were studied produced hemolysins. Among the non-albicans Candidaspecies, 86% exhibited hemolytic activity. Only C. guilliermondiiand some C. parapsilosis isolates were negative for this enzyme. In conclusion, most non-albicans Candidaspecies had a similar ability to produce hemolysins when compared to C. albicans. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-12-01 2014-09-30T18:18:33Z 2014-09-30T18:18:33Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S1806-83242013000600007 Brazilian Oral Research. Sociedade Brasileira de Pesquisa Odontológica - SBPqO, v. 27, n. 6, p. 484-489, 2013. 1806-8324 http://hdl.handle.net/11449/109662 10.1590/S1806-83242013000600007 S1806-83242013000600484 WOS:000327281300007 S1806-83242013000600484.pdf 1664688357804299 0053567153623569 0322020541055900 |
url |
http://dx.doi.org/10.1590/S1806-83242013000600007 http://hdl.handle.net/11449/109662 |
identifier_str_mv |
Brazilian Oral Research. Sociedade Brasileira de Pesquisa Odontológica - SBPqO, v. 27, n. 6, p. 484-489, 2013. 1806-8324 10.1590/S1806-83242013000600007 S1806-83242013000600484 WOS:000327281300007 S1806-83242013000600484.pdf 1664688357804299 0053567153623569 0322020541055900 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Brazilian Oral Research |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
484-489 application/pdf |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Pesquisa Odontológica (SBPqO) |
publisher.none.fl_str_mv |
Sociedade Brasileira de Pesquisa Odontológica (SBPqO) |
dc.source.none.fl_str_mv |
SciELO reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1797789813784444928 |