TGFA/Taq I polymorphism and environmental factors in non-syndromic oral clefts in Southern Brazil
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Oral Research |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242012000500009 |
Resumo: | We report a study of TGFA/ Taq I polymorphisms and environmental factors in non-syndromic oral cleft in Southern Brazil. Nonsyndromic cleft case-parent triads were recruited to participate. Clinical data was collected with an emphasis on tobacco and alcohol use during pregnancy. DNA was extracted from peripheral blood and TGFA/ Taq I polymorphisms were analyzed by PCR/RFLP with Taq I restriction enzyme. Association of clefts and TGFA/ Taq I polymorphisms was determined using a transmission disequilibrium test (TDT). Association of environmental factors, clefts, and genotypes was evaluated with Fisher's exact test. The minor allele frequency was 0.064. We found no evidence of association between TGFA/ Taq I polymorphisms and clefting (TDT p = 0.335). We also found no association between TGFA/ TaqI polymorphisms and environmental factors (alcohol and/or tobacco). Therefore, no evidence was found that TGFA/ Taq I polymorphisms play a role in clefting in this population. No evidence was found that tobacco or alcohol exposure during pregnancy was related to clefting, however a larger sample size is needed to confirm these results. |
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Brazilian Oral Research |
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TGFA/Taq I polymorphism and environmental factors in non-syndromic oral clefts in Southern BrazilCleft LipCleft PalatePolymorphism, GeneticEnvironmental ExposureWe report a study of TGFA/ Taq I polymorphisms and environmental factors in non-syndromic oral cleft in Southern Brazil. Nonsyndromic cleft case-parent triads were recruited to participate. Clinical data was collected with an emphasis on tobacco and alcohol use during pregnancy. DNA was extracted from peripheral blood and TGFA/ Taq I polymorphisms were analyzed by PCR/RFLP with Taq I restriction enzyme. Association of clefts and TGFA/ Taq I polymorphisms was determined using a transmission disequilibrium test (TDT). Association of environmental factors, clefts, and genotypes was evaluated with Fisher's exact test. The minor allele frequency was 0.064. We found no evidence of association between TGFA/ Taq I polymorphisms and clefting (TDT p = 0.335). We also found no association between TGFA/ TaqI polymorphisms and environmental factors (alcohol and/or tobacco). Therefore, no evidence was found that TGFA/ Taq I polymorphisms play a role in clefting in this population. No evidence was found that tobacco or alcohol exposure during pregnancy was related to clefting, however a larger sample size is needed to confirm these results.Sociedade Brasileira de Pesquisa Odontológica - SBPqO2012-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242012000500009Brazilian Oral Research v.26 n.5 2012reponame:Brazilian Oral Researchinstname:Sociedade Brasileira de Pesquisa Odontológica (SBPqO)instacron:SBPQO10.1590/S1806-83242012005000016info:eu-repo/semantics/openAccessSouza,Liliane Todeschini deKowalski,Thayne WoycinckVanz,Ana PaulaGiugliani,RobertoFélix,Têmis Mariaeng2012-09-21T00:00:00Zoai:scielo:S1806-83242012000500009Revistahttps://www.scielo.br/j/bor/https://old.scielo.br/oai/scielo-oai.phppob@edu.usp.br||bor@sbpqo.org.br1807-31071806-8324opendoar:2012-09-21T00:00Brazilian Oral Research - Sociedade Brasileira de Pesquisa Odontológica (SBPqO)false |
dc.title.none.fl_str_mv |
TGFA/Taq I polymorphism and environmental factors in non-syndromic oral clefts in Southern Brazil |
title |
TGFA/Taq I polymorphism and environmental factors in non-syndromic oral clefts in Southern Brazil |
spellingShingle |
TGFA/Taq I polymorphism and environmental factors in non-syndromic oral clefts in Southern Brazil Souza,Liliane Todeschini de Cleft Lip Cleft Palate Polymorphism, Genetic Environmental Exposure |
title_short |
TGFA/Taq I polymorphism and environmental factors in non-syndromic oral clefts in Southern Brazil |
title_full |
TGFA/Taq I polymorphism and environmental factors in non-syndromic oral clefts in Southern Brazil |
title_fullStr |
TGFA/Taq I polymorphism and environmental factors in non-syndromic oral clefts in Southern Brazil |
title_full_unstemmed |
TGFA/Taq I polymorphism and environmental factors in non-syndromic oral clefts in Southern Brazil |
title_sort |
TGFA/Taq I polymorphism and environmental factors in non-syndromic oral clefts in Southern Brazil |
author |
Souza,Liliane Todeschini de |
author_facet |
Souza,Liliane Todeschini de Kowalski,Thayne Woycinck Vanz,Ana Paula Giugliani,Roberto Félix,Têmis Maria |
author_role |
author |
author2 |
Kowalski,Thayne Woycinck Vanz,Ana Paula Giugliani,Roberto Félix,Têmis Maria |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Souza,Liliane Todeschini de Kowalski,Thayne Woycinck Vanz,Ana Paula Giugliani,Roberto Félix,Têmis Maria |
dc.subject.por.fl_str_mv |
Cleft Lip Cleft Palate Polymorphism, Genetic Environmental Exposure |
topic |
Cleft Lip Cleft Palate Polymorphism, Genetic Environmental Exposure |
description |
We report a study of TGFA/ Taq I polymorphisms and environmental factors in non-syndromic oral cleft in Southern Brazil. Nonsyndromic cleft case-parent triads were recruited to participate. Clinical data was collected with an emphasis on tobacco and alcohol use during pregnancy. DNA was extracted from peripheral blood and TGFA/ Taq I polymorphisms were analyzed by PCR/RFLP with Taq I restriction enzyme. Association of clefts and TGFA/ Taq I polymorphisms was determined using a transmission disequilibrium test (TDT). Association of environmental factors, clefts, and genotypes was evaluated with Fisher's exact test. The minor allele frequency was 0.064. We found no evidence of association between TGFA/ Taq I polymorphisms and clefting (TDT p = 0.335). We also found no association between TGFA/ TaqI polymorphisms and environmental factors (alcohol and/or tobacco). Therefore, no evidence was found that TGFA/ Taq I polymorphisms play a role in clefting in this population. No evidence was found that tobacco or alcohol exposure during pregnancy was related to clefting, however a larger sample size is needed to confirm these results. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-10-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242012000500009 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242012000500009 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1806-83242012005000016 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Pesquisa Odontológica - SBPqO |
publisher.none.fl_str_mv |
Sociedade Brasileira de Pesquisa Odontológica - SBPqO |
dc.source.none.fl_str_mv |
Brazilian Oral Research v.26 n.5 2012 reponame:Brazilian Oral Research instname:Sociedade Brasileira de Pesquisa Odontológica (SBPqO) instacron:SBPQO |
instname_str |
Sociedade Brasileira de Pesquisa Odontológica (SBPqO) |
instacron_str |
SBPQO |
institution |
SBPQO |
reponame_str |
Brazilian Oral Research |
collection |
Brazilian Oral Research |
repository.name.fl_str_mv |
Brazilian Oral Research - Sociedade Brasileira de Pesquisa Odontológica (SBPqO) |
repository.mail.fl_str_mv |
pob@edu.usp.br||bor@sbpqo.org.br |
_version_ |
1750318323015352320 |