High Throughput Mass Spectrometry Platform for Prospecting High Value Chemicals from Bioconversion of Glycerin
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Journal of the Brazilian Chemical Society (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532019001202712 |
Resumo: | The use of bioprocess to convert low valued biomass or agroindustrial byproducts into high-value chemicals is an emerging area. However, laboratories usually use outdated analytical techniques to identify bioproducts, losing valuable information, such as the identification of unexpected compounds. In this work, we presented a successful systematic and modern analytical approach based on direct infusion mass spectrometry (DIMS) for an automated high-throughput screening of untargeted compounds from glycerin bioconversion process. We describe advantages of DIMS and its combined application with chemometrics towards an untargeted metabolomics approach to analyze several samples in a short time (11 samples per h). Three batches with 34 samples from the bioconversion of glycerin using several filamentous fungi strains were analyzed. Batch 347 was selected as promising, since high value chemicals, such as amide, phenolic and acid compounds, were identified. The platform presented was fast, robust, and versatile, then it could be applied to different bioprocesses. |
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High Throughput Mass Spectrometry Platform for Prospecting High Value Chemicals from Bioconversion of Glycerinmetabolomicsmass spectrometryDIMSbiotechnologyfilamentous fungiThe use of bioprocess to convert low valued biomass or agroindustrial byproducts into high-value chemicals is an emerging area. However, laboratories usually use outdated analytical techniques to identify bioproducts, losing valuable information, such as the identification of unexpected compounds. In this work, we presented a successful systematic and modern analytical approach based on direct infusion mass spectrometry (DIMS) for an automated high-throughput screening of untargeted compounds from glycerin bioconversion process. We describe advantages of DIMS and its combined application with chemometrics towards an untargeted metabolomics approach to analyze several samples in a short time (11 samples per h). Three batches with 34 samples from the bioconversion of glycerin using several filamentous fungi strains were analyzed. Batch 347 was selected as promising, since high value chemicals, such as amide, phenolic and acid compounds, were identified. The platform presented was fast, robust, and versatile, then it could be applied to different bioprocesses.Sociedade Brasileira de Química2019-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532019001202712Journal of the Brazilian Chemical Society v.30 n.12 2019reponame:Journal of the Brazilian Chemical Society (Online)instname:Sociedade Brasileira de Química (SBQ)instacron:SBQ10.21577/0103-5053.20190210info:eu-repo/semantics/openAccessAmazonas,Diana R.Souto,Augusto L.Vieira,Flávia S.Carneiro,Andréia A. J.Vianna,Rebeca B.Mendes,Thaís D.Lima,Karen S.Ribeiro,José A. A.Damaso,Mônica C. T.Rodrigues,Clenilson M.Abdelnur,Patrícia V.eng2019-10-21T00:00:00Zoai:scielo:S0103-50532019001202712Revistahttp://jbcs.sbq.org.brONGhttps://old.scielo.br/oai/scielo-oai.php||office@jbcs.sbq.org.br1678-47900103-5053opendoar:2019-10-21T00:00Journal of the Brazilian Chemical Society (Online) - Sociedade Brasileira de Química (SBQ)false |
dc.title.none.fl_str_mv |
High Throughput Mass Spectrometry Platform for Prospecting High Value Chemicals from Bioconversion of Glycerin |
title |
High Throughput Mass Spectrometry Platform for Prospecting High Value Chemicals from Bioconversion of Glycerin |
spellingShingle |
High Throughput Mass Spectrometry Platform for Prospecting High Value Chemicals from Bioconversion of Glycerin Amazonas,Diana R. metabolomics mass spectrometry DIMS biotechnology filamentous fungi |
title_short |
High Throughput Mass Spectrometry Platform for Prospecting High Value Chemicals from Bioconversion of Glycerin |
title_full |
High Throughput Mass Spectrometry Platform for Prospecting High Value Chemicals from Bioconversion of Glycerin |
title_fullStr |
High Throughput Mass Spectrometry Platform for Prospecting High Value Chemicals from Bioconversion of Glycerin |
title_full_unstemmed |
High Throughput Mass Spectrometry Platform for Prospecting High Value Chemicals from Bioconversion of Glycerin |
title_sort |
High Throughput Mass Spectrometry Platform for Prospecting High Value Chemicals from Bioconversion of Glycerin |
author |
Amazonas,Diana R. |
author_facet |
Amazonas,Diana R. Souto,Augusto L. Vieira,Flávia S. Carneiro,Andréia A. J. Vianna,Rebeca B. Mendes,Thaís D. Lima,Karen S. Ribeiro,José A. A. Damaso,Mônica C. T. Rodrigues,Clenilson M. Abdelnur,Patrícia V. |
author_role |
author |
author2 |
Souto,Augusto L. Vieira,Flávia S. Carneiro,Andréia A. J. Vianna,Rebeca B. Mendes,Thaís D. Lima,Karen S. Ribeiro,José A. A. Damaso,Mônica C. T. Rodrigues,Clenilson M. Abdelnur,Patrícia V. |
author2_role |
author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Amazonas,Diana R. Souto,Augusto L. Vieira,Flávia S. Carneiro,Andréia A. J. Vianna,Rebeca B. Mendes,Thaís D. Lima,Karen S. Ribeiro,José A. A. Damaso,Mônica C. T. Rodrigues,Clenilson M. Abdelnur,Patrícia V. |
dc.subject.por.fl_str_mv |
metabolomics mass spectrometry DIMS biotechnology filamentous fungi |
topic |
metabolomics mass spectrometry DIMS biotechnology filamentous fungi |
description |
The use of bioprocess to convert low valued biomass or agroindustrial byproducts into high-value chemicals is an emerging area. However, laboratories usually use outdated analytical techniques to identify bioproducts, losing valuable information, such as the identification of unexpected compounds. In this work, we presented a successful systematic and modern analytical approach based on direct infusion mass spectrometry (DIMS) for an automated high-throughput screening of untargeted compounds from glycerin bioconversion process. We describe advantages of DIMS and its combined application with chemometrics towards an untargeted metabolomics approach to analyze several samples in a short time (11 samples per h). Three batches with 34 samples from the bioconversion of glycerin using several filamentous fungi strains were analyzed. Batch 347 was selected as promising, since high value chemicals, such as amide, phenolic and acid compounds, were identified. The platform presented was fast, robust, and versatile, then it could be applied to different bioprocesses. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-12-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532019001202712 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532019001202712 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.21577/0103-5053.20190210 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Química |
publisher.none.fl_str_mv |
Sociedade Brasileira de Química |
dc.source.none.fl_str_mv |
Journal of the Brazilian Chemical Society v.30 n.12 2019 reponame:Journal of the Brazilian Chemical Society (Online) instname:Sociedade Brasileira de Química (SBQ) instacron:SBQ |
instname_str |
Sociedade Brasileira de Química (SBQ) |
instacron_str |
SBQ |
institution |
SBQ |
reponame_str |
Journal of the Brazilian Chemical Society (Online) |
collection |
Journal of the Brazilian Chemical Society (Online) |
repository.name.fl_str_mv |
Journal of the Brazilian Chemical Society (Online) - Sociedade Brasileira de Química (SBQ) |
repository.mail.fl_str_mv |
||office@jbcs.sbq.org.br |
_version_ |
1750318182525042688 |