Investigation of the interaction of sertraline with calf thymus DNA by spectroscopic methods

Detalhes bibliográficos
Autor(a) principal: Dorraji,Parisa S.
Data de Publicação: 2013
Outros Autores: Jalali,Fahimeh
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Journal of the Brazilian Chemical Society (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532013000600007
Resumo: The interaction of the antidepressant drug, sertraline, with calf thymus double stranded DNA (dsDNA) in physiological buffer (pH 7.4) was investigated by UV-Vis spectrophotometry, spectrofluorimetry, circular dichroism, Fourier transform infrared spectroscopy (FTIR), viscosity measurements and DNA melting studies. The absorption spectra of the drug with DNA showed a hyperchromic effect. Using Hoechst reagent as a fluorescence probe, quenching of the emission peak occurred in the DNA-Hoechst mixture when sertraline was added. The FTIR spectra revealed minor groove binding mode between the drug and the dsDNA. The binding constant of sertraline to DNA was calculated using spectroscopic data. The calculated thermodynamic parameters suggested that electrostatic interactions are important forces in the formation of sertraline-DNA complex.
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spelling Investigation of the interaction of sertraline with calf thymus DNA by spectroscopic methodsDNAsertralineinteractionspectroscopyThe interaction of the antidepressant drug, sertraline, with calf thymus double stranded DNA (dsDNA) in physiological buffer (pH 7.4) was investigated by UV-Vis spectrophotometry, spectrofluorimetry, circular dichroism, Fourier transform infrared spectroscopy (FTIR), viscosity measurements and DNA melting studies. The absorption spectra of the drug with DNA showed a hyperchromic effect. Using Hoechst reagent as a fluorescence probe, quenching of the emission peak occurred in the DNA-Hoechst mixture when sertraline was added. The FTIR spectra revealed minor groove binding mode between the drug and the dsDNA. The binding constant of sertraline to DNA was calculated using spectroscopic data. The calculated thermodynamic parameters suggested that electrostatic interactions are important forces in the formation of sertraline-DNA complex.Sociedade Brasileira de Química2013-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532013000600007Journal of the Brazilian Chemical Society v.24 n.6 2013reponame:Journal of the Brazilian Chemical Society (Online)instname:Sociedade Brasileira de Química (SBQ)instacron:SBQ10.5935/0103-5053.20130123info:eu-repo/semantics/openAccessDorraji,Parisa S.Jalali,Fahimeheng2013-06-28T00:00:00Zoai:scielo:S0103-50532013000600007Revistahttp://jbcs.sbq.org.brONGhttps://old.scielo.br/oai/scielo-oai.php||office@jbcs.sbq.org.br1678-47900103-5053opendoar:2013-06-28T00:00Journal of the Brazilian Chemical Society (Online) - Sociedade Brasileira de Química (SBQ)false
dc.title.none.fl_str_mv Investigation of the interaction of sertraline with calf thymus DNA by spectroscopic methods
title Investigation of the interaction of sertraline with calf thymus DNA by spectroscopic methods
spellingShingle Investigation of the interaction of sertraline with calf thymus DNA by spectroscopic methods
Dorraji,Parisa S.
DNA
sertraline
interaction
spectroscopy
title_short Investigation of the interaction of sertraline with calf thymus DNA by spectroscopic methods
title_full Investigation of the interaction of sertraline with calf thymus DNA by spectroscopic methods
title_fullStr Investigation of the interaction of sertraline with calf thymus DNA by spectroscopic methods
title_full_unstemmed Investigation of the interaction of sertraline with calf thymus DNA by spectroscopic methods
title_sort Investigation of the interaction of sertraline with calf thymus DNA by spectroscopic methods
author Dorraji,Parisa S.
author_facet Dorraji,Parisa S.
Jalali,Fahimeh
author_role author
author2 Jalali,Fahimeh
author2_role author
dc.contributor.author.fl_str_mv Dorraji,Parisa S.
Jalali,Fahimeh
dc.subject.por.fl_str_mv DNA
sertraline
interaction
spectroscopy
topic DNA
sertraline
interaction
spectroscopy
description The interaction of the antidepressant drug, sertraline, with calf thymus double stranded DNA (dsDNA) in physiological buffer (pH 7.4) was investigated by UV-Vis spectrophotometry, spectrofluorimetry, circular dichroism, Fourier transform infrared spectroscopy (FTIR), viscosity measurements and DNA melting studies. The absorption spectra of the drug with DNA showed a hyperchromic effect. Using Hoechst reagent as a fluorescence probe, quenching of the emission peak occurred in the DNA-Hoechst mixture when sertraline was added. The FTIR spectra revealed minor groove binding mode between the drug and the dsDNA. The binding constant of sertraline to DNA was calculated using spectroscopic data. The calculated thermodynamic parameters suggested that electrostatic interactions are important forces in the formation of sertraline-DNA complex.
publishDate 2013
dc.date.none.fl_str_mv 2013-06-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532013000600007
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532013000600007
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.5935/0103-5053.20130123
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Química
publisher.none.fl_str_mv Sociedade Brasileira de Química
dc.source.none.fl_str_mv Journal of the Brazilian Chemical Society v.24 n.6 2013
reponame:Journal of the Brazilian Chemical Society (Online)
instname:Sociedade Brasileira de Química (SBQ)
instacron:SBQ
instname_str Sociedade Brasileira de Química (SBQ)
instacron_str SBQ
institution SBQ
reponame_str Journal of the Brazilian Chemical Society (Online)
collection Journal of the Brazilian Chemical Society (Online)
repository.name.fl_str_mv Journal of the Brazilian Chemical Society (Online) - Sociedade Brasileira de Química (SBQ)
repository.mail.fl_str_mv ||office@jbcs.sbq.org.br
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