Downregulation of C3 and C4A/B complement factor fragments in plasma from patients with squamous cell carcinoma of the penis

Detalhes bibliográficos
Autor(a) principal: Ornellas,Paulo
Data de Publicação: 2012
Outros Autores: Ornellas,Antonio Augusto, Chinello,Clizia, Gianazza,Erica, Mainini,Veronica, Cazzaniga,Marta, Pereira,Denise Abreu, Sandim,Vanessa, Cypriano,Ana Sheila, Koifman,Leandro, Silva,Paulo Cesar Barbosa da, Alves,Gilda, Magni,Fulvio
Tipo de documento: Artigo
Idioma: eng
Título da fonte: International Braz J Urol (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1677-55382012000600739
Resumo: Purpose To investigate the use of ClinProt technique to identify cancer markers in plasma of patients suffering from squamous cell carcinoma of the penis (SCCP). Materials and Methods Plasma of 36 healthy subjects and 25 patients with penile carcinoma who underwent surgical treatment between June 2010 and June 2011 was collected and analyzed by the ClinProt/MALDI/ToF technique. Then the peptides were identified from the C8 MB eluted fraction of patients' and control subjects' plasma by LIFT MS/MS. Results A cluster of 2 peptides (A=m/z 1897.22 ± 9 Da and B=m/z 2021.99 ± 9 Da) was able to discriminate patients from control subjects. Cross validation analysis using the whole casuistic showed 62.5% and 86.76% sensitivity and specificity, respectively. The cluster also showed very high sensitivity (100%) and specificity (97%) for SCCP patients that died due to the disease. Furthermore, patients with lymph node involvement presented sensitivity and specificity of 80% and 97%, respectively. These two peptides were identified by the proteomic approach based on a MALDI-TOF/TOF as fragments of C3 (m/z 1896.17) and C4a/b (m/z 2021.26) complement proteins. Conclusions The results showed that as the disease progresses, the fragments C3 and C4 A/B are less expressed in comparison with healthy subjects. These results may be useful as prognostic tools.
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spelling Downregulation of C3 and C4A/B complement factor fragments in plasma from patients with squamous cell carcinoma of the penisPenisPenis CancerCarcinoma, Squamous CellPlasmaProteomicsComplement C3b Inactivator Proteins Purpose To investigate the use of ClinProt technique to identify cancer markers in plasma of patients suffering from squamous cell carcinoma of the penis (SCCP). Materials and Methods Plasma of 36 healthy subjects and 25 patients with penile carcinoma who underwent surgical treatment between June 2010 and June 2011 was collected and analyzed by the ClinProt/MALDI/ToF technique. Then the peptides were identified from the C8 MB eluted fraction of patients' and control subjects' plasma by LIFT MS/MS. Results A cluster of 2 peptides (A=m/z 1897.22 ± 9 Da and B=m/z 2021.99 ± 9 Da) was able to discriminate patients from control subjects. Cross validation analysis using the whole casuistic showed 62.5% and 86.76% sensitivity and specificity, respectively. The cluster also showed very high sensitivity (100%) and specificity (97%) for SCCP patients that died due to the disease. Furthermore, patients with lymph node involvement presented sensitivity and specificity of 80% and 97%, respectively. These two peptides were identified by the proteomic approach based on a MALDI-TOF/TOF as fragments of C3 (m/z 1896.17) and C4a/b (m/z 2021.26) complement proteins. Conclusions The results showed that as the disease progresses, the fragments C3 and C4 A/B are less expressed in comparison with healthy subjects. These results may be useful as prognostic tools. Sociedade Brasileira de Urologia2012-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1677-55382012000600739International braz j urol v.38 n.6 2012reponame:International Braz J Urol (Online)instname:Sociedade Brasileira de Urologia (SBU)instacron:SBU10.1590/1677-553820133806739info:eu-repo/semantics/openAccessOrnellas,PauloOrnellas,Antonio AugustoChinello,CliziaGianazza,EricaMainini,VeronicaCazzaniga,MartaPereira,Denise AbreuSandim,VanessaCypriano,Ana SheilaKoifman,LeandroSilva,Paulo Cesar Barbosa daAlves,GildaMagni,Fulvioeng2013-04-05T00:00:00Zoai:scielo:S1677-55382012000600739Revistahttp://www.brazjurol.com.br/ONGhttps://old.scielo.br/oai/scielo-oai.php||brazjurol@brazjurol.com.br1677-61191677-5538opendoar:2013-04-05T00:00International Braz J Urol (Online) - Sociedade Brasileira de Urologia (SBU)false
dc.title.none.fl_str_mv Downregulation of C3 and C4A/B complement factor fragments in plasma from patients with squamous cell carcinoma of the penis
title Downregulation of C3 and C4A/B complement factor fragments in plasma from patients with squamous cell carcinoma of the penis
spellingShingle Downregulation of C3 and C4A/B complement factor fragments in plasma from patients with squamous cell carcinoma of the penis
Ornellas,Paulo
Penis
Penis Cancer
Carcinoma, Squamous Cell
Plasma
Proteomics
Complement C3b Inactivator Proteins
title_short Downregulation of C3 and C4A/B complement factor fragments in plasma from patients with squamous cell carcinoma of the penis
title_full Downregulation of C3 and C4A/B complement factor fragments in plasma from patients with squamous cell carcinoma of the penis
title_fullStr Downregulation of C3 and C4A/B complement factor fragments in plasma from patients with squamous cell carcinoma of the penis
title_full_unstemmed Downregulation of C3 and C4A/B complement factor fragments in plasma from patients with squamous cell carcinoma of the penis
title_sort Downregulation of C3 and C4A/B complement factor fragments in plasma from patients with squamous cell carcinoma of the penis
author Ornellas,Paulo
author_facet Ornellas,Paulo
Ornellas,Antonio Augusto
Chinello,Clizia
Gianazza,Erica
Mainini,Veronica
Cazzaniga,Marta
Pereira,Denise Abreu
Sandim,Vanessa
Cypriano,Ana Sheila
Koifman,Leandro
Silva,Paulo Cesar Barbosa da
Alves,Gilda
Magni,Fulvio
author_role author
author2 Ornellas,Antonio Augusto
Chinello,Clizia
Gianazza,Erica
Mainini,Veronica
Cazzaniga,Marta
Pereira,Denise Abreu
Sandim,Vanessa
Cypriano,Ana Sheila
Koifman,Leandro
Silva,Paulo Cesar Barbosa da
Alves,Gilda
Magni,Fulvio
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Ornellas,Paulo
Ornellas,Antonio Augusto
Chinello,Clizia
Gianazza,Erica
Mainini,Veronica
Cazzaniga,Marta
Pereira,Denise Abreu
Sandim,Vanessa
Cypriano,Ana Sheila
Koifman,Leandro
Silva,Paulo Cesar Barbosa da
Alves,Gilda
Magni,Fulvio
dc.subject.por.fl_str_mv Penis
Penis Cancer
Carcinoma, Squamous Cell
Plasma
Proteomics
Complement C3b Inactivator Proteins
topic Penis
Penis Cancer
Carcinoma, Squamous Cell
Plasma
Proteomics
Complement C3b Inactivator Proteins
description Purpose To investigate the use of ClinProt technique to identify cancer markers in plasma of patients suffering from squamous cell carcinoma of the penis (SCCP). Materials and Methods Plasma of 36 healthy subjects and 25 patients with penile carcinoma who underwent surgical treatment between June 2010 and June 2011 was collected and analyzed by the ClinProt/MALDI/ToF technique. Then the peptides were identified from the C8 MB eluted fraction of patients' and control subjects' plasma by LIFT MS/MS. Results A cluster of 2 peptides (A=m/z 1897.22 ± 9 Da and B=m/z 2021.99 ± 9 Da) was able to discriminate patients from control subjects. Cross validation analysis using the whole casuistic showed 62.5% and 86.76% sensitivity and specificity, respectively. The cluster also showed very high sensitivity (100%) and specificity (97%) for SCCP patients that died due to the disease. Furthermore, patients with lymph node involvement presented sensitivity and specificity of 80% and 97%, respectively. These two peptides were identified by the proteomic approach based on a MALDI-TOF/TOF as fragments of C3 (m/z 1896.17) and C4a/b (m/z 2021.26) complement proteins. Conclusions The results showed that as the disease progresses, the fragments C3 and C4 A/B are less expressed in comparison with healthy subjects. These results may be useful as prognostic tools.
publishDate 2012
dc.date.none.fl_str_mv 2012-12-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1677-55382012000600739
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1677-55382012000600739
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/1677-553820133806739
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Urologia
publisher.none.fl_str_mv Sociedade Brasileira de Urologia
dc.source.none.fl_str_mv International braz j urol v.38 n.6 2012
reponame:International Braz J Urol (Online)
instname:Sociedade Brasileira de Urologia (SBU)
instacron:SBU
instname_str Sociedade Brasileira de Urologia (SBU)
instacron_str SBU
institution SBU
reponame_str International Braz J Urol (Online)
collection International Braz J Urol (Online)
repository.name.fl_str_mv International Braz J Urol (Online) - Sociedade Brasileira de Urologia (SBU)
repository.mail.fl_str_mv ||brazjurol@brazjurol.com.br
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