Downregulation of C3 and C4A/B complement factor fragments in plasma from patients with squamous cell carcinoma of the penis
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | International Braz J Urol (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1677-55382012000600739 |
Resumo: | Purpose To investigate the use of ClinProt technique to identify cancer markers in plasma of patients suffering from squamous cell carcinoma of the penis (SCCP). Materials and Methods Plasma of 36 healthy subjects and 25 patients with penile carcinoma who underwent surgical treatment between June 2010 and June 2011 was collected and analyzed by the ClinProt/MALDI/ToF technique. Then the peptides were identified from the C8 MB eluted fraction of patients' and control subjects' plasma by LIFT MS/MS. Results A cluster of 2 peptides (A=m/z 1897.22 ± 9 Da and B=m/z 2021.99 ± 9 Da) was able to discriminate patients from control subjects. Cross validation analysis using the whole casuistic showed 62.5% and 86.76% sensitivity and specificity, respectively. The cluster also showed very high sensitivity (100%) and specificity (97%) for SCCP patients that died due to the disease. Furthermore, patients with lymph node involvement presented sensitivity and specificity of 80% and 97%, respectively. These two peptides were identified by the proteomic approach based on a MALDI-TOF/TOF as fragments of C3 (m/z 1896.17) and C4a/b (m/z 2021.26) complement proteins. Conclusions The results showed that as the disease progresses, the fragments C3 and C4 A/B are less expressed in comparison with healthy subjects. These results may be useful as prognostic tools. |
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International Braz J Urol (Online) |
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Downregulation of C3 and C4A/B complement factor fragments in plasma from patients with squamous cell carcinoma of the penisPenisPenis CancerCarcinoma, Squamous CellPlasmaProteomicsComplement C3b Inactivator Proteins Purpose To investigate the use of ClinProt technique to identify cancer markers in plasma of patients suffering from squamous cell carcinoma of the penis (SCCP). Materials and Methods Plasma of 36 healthy subjects and 25 patients with penile carcinoma who underwent surgical treatment between June 2010 and June 2011 was collected and analyzed by the ClinProt/MALDI/ToF technique. Then the peptides were identified from the C8 MB eluted fraction of patients' and control subjects' plasma by LIFT MS/MS. Results A cluster of 2 peptides (A=m/z 1897.22 ± 9 Da and B=m/z 2021.99 ± 9 Da) was able to discriminate patients from control subjects. Cross validation analysis using the whole casuistic showed 62.5% and 86.76% sensitivity and specificity, respectively. The cluster also showed very high sensitivity (100%) and specificity (97%) for SCCP patients that died due to the disease. Furthermore, patients with lymph node involvement presented sensitivity and specificity of 80% and 97%, respectively. These two peptides were identified by the proteomic approach based on a MALDI-TOF/TOF as fragments of C3 (m/z 1896.17) and C4a/b (m/z 2021.26) complement proteins. Conclusions The results showed that as the disease progresses, the fragments C3 and C4 A/B are less expressed in comparison with healthy subjects. These results may be useful as prognostic tools. Sociedade Brasileira de Urologia2012-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1677-55382012000600739International braz j urol v.38 n.6 2012reponame:International Braz J Urol (Online)instname:Sociedade Brasileira de Urologia (SBU)instacron:SBU10.1590/1677-553820133806739info:eu-repo/semantics/openAccessOrnellas,PauloOrnellas,Antonio AugustoChinello,CliziaGianazza,EricaMainini,VeronicaCazzaniga,MartaPereira,Denise AbreuSandim,VanessaCypriano,Ana SheilaKoifman,LeandroSilva,Paulo Cesar Barbosa daAlves,GildaMagni,Fulvioeng2013-04-05T00:00:00Zoai:scielo:S1677-55382012000600739Revistahttp://www.brazjurol.com.br/ONGhttps://old.scielo.br/oai/scielo-oai.php||brazjurol@brazjurol.com.br1677-61191677-5538opendoar:2013-04-05T00:00International Braz J Urol (Online) - Sociedade Brasileira de Urologia (SBU)false |
dc.title.none.fl_str_mv |
Downregulation of C3 and C4A/B complement factor fragments in plasma from patients with squamous cell carcinoma of the penis |
title |
Downregulation of C3 and C4A/B complement factor fragments in plasma from patients with squamous cell carcinoma of the penis |
spellingShingle |
Downregulation of C3 and C4A/B complement factor fragments in plasma from patients with squamous cell carcinoma of the penis Ornellas,Paulo Penis Penis Cancer Carcinoma, Squamous Cell Plasma Proteomics Complement C3b Inactivator Proteins |
title_short |
Downregulation of C3 and C4A/B complement factor fragments in plasma from patients with squamous cell carcinoma of the penis |
title_full |
Downregulation of C3 and C4A/B complement factor fragments in plasma from patients with squamous cell carcinoma of the penis |
title_fullStr |
Downregulation of C3 and C4A/B complement factor fragments in plasma from patients with squamous cell carcinoma of the penis |
title_full_unstemmed |
Downregulation of C3 and C4A/B complement factor fragments in plasma from patients with squamous cell carcinoma of the penis |
title_sort |
Downregulation of C3 and C4A/B complement factor fragments in plasma from patients with squamous cell carcinoma of the penis |
author |
Ornellas,Paulo |
author_facet |
Ornellas,Paulo Ornellas,Antonio Augusto Chinello,Clizia Gianazza,Erica Mainini,Veronica Cazzaniga,Marta Pereira,Denise Abreu Sandim,Vanessa Cypriano,Ana Sheila Koifman,Leandro Silva,Paulo Cesar Barbosa da Alves,Gilda Magni,Fulvio |
author_role |
author |
author2 |
Ornellas,Antonio Augusto Chinello,Clizia Gianazza,Erica Mainini,Veronica Cazzaniga,Marta Pereira,Denise Abreu Sandim,Vanessa Cypriano,Ana Sheila Koifman,Leandro Silva,Paulo Cesar Barbosa da Alves,Gilda Magni,Fulvio |
author2_role |
author author author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Ornellas,Paulo Ornellas,Antonio Augusto Chinello,Clizia Gianazza,Erica Mainini,Veronica Cazzaniga,Marta Pereira,Denise Abreu Sandim,Vanessa Cypriano,Ana Sheila Koifman,Leandro Silva,Paulo Cesar Barbosa da Alves,Gilda Magni,Fulvio |
dc.subject.por.fl_str_mv |
Penis Penis Cancer Carcinoma, Squamous Cell Plasma Proteomics Complement C3b Inactivator Proteins |
topic |
Penis Penis Cancer Carcinoma, Squamous Cell Plasma Proteomics Complement C3b Inactivator Proteins |
description |
Purpose To investigate the use of ClinProt technique to identify cancer markers in plasma of patients suffering from squamous cell carcinoma of the penis (SCCP). Materials and Methods Plasma of 36 healthy subjects and 25 patients with penile carcinoma who underwent surgical treatment between June 2010 and June 2011 was collected and analyzed by the ClinProt/MALDI/ToF technique. Then the peptides were identified from the C8 MB eluted fraction of patients' and control subjects' plasma by LIFT MS/MS. Results A cluster of 2 peptides (A=m/z 1897.22 ± 9 Da and B=m/z 2021.99 ± 9 Da) was able to discriminate patients from control subjects. Cross validation analysis using the whole casuistic showed 62.5% and 86.76% sensitivity and specificity, respectively. The cluster also showed very high sensitivity (100%) and specificity (97%) for SCCP patients that died due to the disease. Furthermore, patients with lymph node involvement presented sensitivity and specificity of 80% and 97%, respectively. These two peptides were identified by the proteomic approach based on a MALDI-TOF/TOF as fragments of C3 (m/z 1896.17) and C4a/b (m/z 2021.26) complement proteins. Conclusions The results showed that as the disease progresses, the fragments C3 and C4 A/B are less expressed in comparison with healthy subjects. These results may be useful as prognostic tools. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-12-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1677-55382012000600739 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1677-55382012000600739 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/1677-553820133806739 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Urologia |
publisher.none.fl_str_mv |
Sociedade Brasileira de Urologia |
dc.source.none.fl_str_mv |
International braz j urol v.38 n.6 2012 reponame:International Braz J Urol (Online) instname:Sociedade Brasileira de Urologia (SBU) instacron:SBU |
instname_str |
Sociedade Brasileira de Urologia (SBU) |
instacron_str |
SBU |
institution |
SBU |
reponame_str |
International Braz J Urol (Online) |
collection |
International Braz J Urol (Online) |
repository.name.fl_str_mv |
International Braz J Urol (Online) - Sociedade Brasileira de Urologia (SBU) |
repository.mail.fl_str_mv |
||brazjurol@brazjurol.com.br |
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1750318072837701632 |