Paraoxonase activity in liver of Pacu, Piaractus mesopotamicus Holmberg (Characidae)
Autor(a) principal: | |
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Data de Publicação: | 1998 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista Brasileira de Zoologia (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-81751998000300012 |
Resumo: | Enzymatic production of p-nitrophenol in liver of Piaractus mesopotamicus Holmberg, 1887 was consistently assayed at pH 8.5 using 7.5 mM paraoxon as substrate. This pacu liver paraoxonase activity was activated by NaCl. Apparent values of K M were 2.42 x 10-3 M in the presence of 0.5 M NaCl and 8.99 x 10-3 M without NaCl. Apparent maximum velocity values calculated in the absence and presence of 0.5 M NaCl were 1.09 x 10-3 µmoles/min/mg of proteins and 1.29 x 100-3 µmoles/min/mg of proteins, respectively. These Vmax values are fifty-fold the value described for trout (Salmo trutta Linnaeus, 1758) liver paraoxonase. Paraoxonase activity of pacu liver homogenates was recovered as much in cytosolic as in particulate cellular subtractions, but the particulate subtractions showed higher specific activities. The data presented here indicate that hepatic hydrolysis of organophosphorous pesticides may not be an important detoxification process in pacu. |
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Paraoxonase activity in liver of Pacu, Piaractus mesopotamicus Holmberg (Characidae)DetoxicationesterasefishorganophosphateEnzymatic production of p-nitrophenol in liver of Piaractus mesopotamicus Holmberg, 1887 was consistently assayed at pH 8.5 using 7.5 mM paraoxon as substrate. This pacu liver paraoxonase activity was activated by NaCl. Apparent values of K M were 2.42 x 10-3 M in the presence of 0.5 M NaCl and 8.99 x 10-3 M without NaCl. Apparent maximum velocity values calculated in the absence and presence of 0.5 M NaCl were 1.09 x 10-3 µmoles/min/mg of proteins and 1.29 x 100-3 µmoles/min/mg of proteins, respectively. These Vmax values are fifty-fold the value described for trout (Salmo trutta Linnaeus, 1758) liver paraoxonase. Paraoxonase activity of pacu liver homogenates was recovered as much in cytosolic as in particulate cellular subtractions, but the particulate subtractions showed higher specific activities. The data presented here indicate that hepatic hydrolysis of organophosphorous pesticides may not be an important detoxification process in pacu.Sociedade Brasileira de Zoologia1998-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-81751998000300012Revista Brasileira de Zoologia v.15 n.3 1998reponame:Revista Brasileira de Zoologia (Online)instname:Sociedade Brasileira de Zoologia (SBZ)instacron:SBZ10.1590/S0101-81751998000300012info:eu-repo/semantics/openAccessBastos,Vera Lucia F. CunhaFolly,EvelizeRossini,AnaCeccarelli,Paulo S.Senhorini,José A.Bastos,Jayme Cunhaeng2009-07-10T00:00:00Zoai:scielo:S0101-81751998000300012Revistahttp://calvados.c3sl.ufpr.br/ojs2/index.php/zooONGhttps://old.scielo.br/oai/scielo-oai.php||sbz@bio.ufpr.br1806-969X0101-8175opendoar:2009-07-10T00:00Revista Brasileira de Zoologia (Online) - Sociedade Brasileira de Zoologia (SBZ)false |
dc.title.none.fl_str_mv |
Paraoxonase activity in liver of Pacu, Piaractus mesopotamicus Holmberg (Characidae) |
title |
Paraoxonase activity in liver of Pacu, Piaractus mesopotamicus Holmberg (Characidae) |
spellingShingle |
Paraoxonase activity in liver of Pacu, Piaractus mesopotamicus Holmberg (Characidae) Bastos,Vera Lucia F. Cunha Detoxication esterase fish organophosphate |
title_short |
Paraoxonase activity in liver of Pacu, Piaractus mesopotamicus Holmberg (Characidae) |
title_full |
Paraoxonase activity in liver of Pacu, Piaractus mesopotamicus Holmberg (Characidae) |
title_fullStr |
Paraoxonase activity in liver of Pacu, Piaractus mesopotamicus Holmberg (Characidae) |
title_full_unstemmed |
Paraoxonase activity in liver of Pacu, Piaractus mesopotamicus Holmberg (Characidae) |
title_sort |
Paraoxonase activity in liver of Pacu, Piaractus mesopotamicus Holmberg (Characidae) |
author |
Bastos,Vera Lucia F. Cunha |
author_facet |
Bastos,Vera Lucia F. Cunha Folly,Evelize Rossini,Ana Ceccarelli,Paulo S. Senhorini,José A. Bastos,Jayme Cunha |
author_role |
author |
author2 |
Folly,Evelize Rossini,Ana Ceccarelli,Paulo S. Senhorini,José A. Bastos,Jayme Cunha |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Bastos,Vera Lucia F. Cunha Folly,Evelize Rossini,Ana Ceccarelli,Paulo S. Senhorini,José A. Bastos,Jayme Cunha |
dc.subject.por.fl_str_mv |
Detoxication esterase fish organophosphate |
topic |
Detoxication esterase fish organophosphate |
description |
Enzymatic production of p-nitrophenol in liver of Piaractus mesopotamicus Holmberg, 1887 was consistently assayed at pH 8.5 using 7.5 mM paraoxon as substrate. This pacu liver paraoxonase activity was activated by NaCl. Apparent values of K M were 2.42 x 10-3 M in the presence of 0.5 M NaCl and 8.99 x 10-3 M without NaCl. Apparent maximum velocity values calculated in the absence and presence of 0.5 M NaCl were 1.09 x 10-3 µmoles/min/mg of proteins and 1.29 x 100-3 µmoles/min/mg of proteins, respectively. These Vmax values are fifty-fold the value described for trout (Salmo trutta Linnaeus, 1758) liver paraoxonase. Paraoxonase activity of pacu liver homogenates was recovered as much in cytosolic as in particulate cellular subtractions, but the particulate subtractions showed higher specific activities. The data presented here indicate that hepatic hydrolysis of organophosphorous pesticides may not be an important detoxification process in pacu. |
publishDate |
1998 |
dc.date.none.fl_str_mv |
1998-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-81751998000300012 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-81751998000300012 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0101-81751998000300012 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Zoologia |
publisher.none.fl_str_mv |
Sociedade Brasileira de Zoologia |
dc.source.none.fl_str_mv |
Revista Brasileira de Zoologia v.15 n.3 1998 reponame:Revista Brasileira de Zoologia (Online) instname:Sociedade Brasileira de Zoologia (SBZ) instacron:SBZ |
instname_str |
Sociedade Brasileira de Zoologia (SBZ) |
instacron_str |
SBZ |
institution |
SBZ |
reponame_str |
Revista Brasileira de Zoologia (Online) |
collection |
Revista Brasileira de Zoologia (Online) |
repository.name.fl_str_mv |
Revista Brasileira de Zoologia (Online) - Sociedade Brasileira de Zoologia (SBZ) |
repository.mail.fl_str_mv |
||sbz@bio.ufpr.br |
_version_ |
1754820981041397760 |