Paraoxonase activity in sera from Piaractus mesopotamicus Holmberg (Characidae) and Hypostomus punctatus Valenciennes (Siluridae)

Detalhes bibliográficos
Autor(a) principal: Bastos,Vera Lucia F. Cunha
Data de Publicação: 1998
Outros Autores: Rossini,Ana, Alves,Marcelo V, Ceccarelli,Paulo S, de Lima,José A. Ferraz, Bastos,Jayme Cunha
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista Brasileira de Zoologia (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-81751998000300011
Resumo: A paraoxonase activity present in serum of two Brazilian fish species was consistently assayed at pH 8.5 using 7.5 mM paraoxon final concentration. The paraoxonase activity was more activated by 0.5 M NaCl in serum of Piaractus mesopotanricus Holmberg, 1887 (pacu) than in serum of Hypostomus punctatus Valenciennes, 1840 (cascudo). Apparent values of K M were 3.3 x 10-3 M for cascudo and pacu paraoxonase activity in the presence of 0.5 M NaCl. Apparent maximum velocity values calculated in the presence of 0.5 M NaCl were 6.1 and 6.5 nmole/min/mL of serum for cascudo and pacu, respectively. Vmax/K M ratio values of determinations in the presence and absence of 0.5 M NaCl showed that NaCl had a more evident effect on increasing the affinity of serum paraoxonase for paraoxon in pacu serum. Young specimens of pacu showed a marked decreased paraoxonase serum activity when kept in tanks treated with 0.25 ppm methyl-parathion.
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spelling Paraoxonase activity in sera from Piaractus mesopotamicus Holmberg (Characidae) and Hypostomus punctatus Valenciennes (Siluridae)Detoxicationfishorganophosphateserum esterasesA paraoxonase activity present in serum of two Brazilian fish species was consistently assayed at pH 8.5 using 7.5 mM paraoxon final concentration. The paraoxonase activity was more activated by 0.5 M NaCl in serum of Piaractus mesopotanricus Holmberg, 1887 (pacu) than in serum of Hypostomus punctatus Valenciennes, 1840 (cascudo). Apparent values of K M were 3.3 x 10-3 M for cascudo and pacu paraoxonase activity in the presence of 0.5 M NaCl. Apparent maximum velocity values calculated in the presence of 0.5 M NaCl were 6.1 and 6.5 nmole/min/mL of serum for cascudo and pacu, respectively. Vmax/K M ratio values of determinations in the presence and absence of 0.5 M NaCl showed that NaCl had a more evident effect on increasing the affinity of serum paraoxonase for paraoxon in pacu serum. Young specimens of pacu showed a marked decreased paraoxonase serum activity when kept in tanks treated with 0.25 ppm methyl-parathion.Sociedade Brasileira de Zoologia1998-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-81751998000300011Revista Brasileira de Zoologia v.15 n.3 1998reponame:Revista Brasileira de Zoologia (Online)instname:Sociedade Brasileira de Zoologia (SBZ)instacron:SBZ10.1590/S0101-81751998000300011info:eu-repo/semantics/openAccessBastos,Vera Lucia F. CunhaRossini,AnaAlves,Marcelo VCeccarelli,Paulo Sde Lima,José A. FerrazBastos,Jayme Cunhaeng2009-07-10T00:00:00Zoai:scielo:S0101-81751998000300011Revistahttp://calvados.c3sl.ufpr.br/ojs2/index.php/zooONGhttps://old.scielo.br/oai/scielo-oai.php||sbz@bio.ufpr.br1806-969X0101-8175opendoar:2009-07-10T00:00Revista Brasileira de Zoologia (Online) - Sociedade Brasileira de Zoologia (SBZ)false
dc.title.none.fl_str_mv Paraoxonase activity in sera from Piaractus mesopotamicus Holmberg (Characidae) and Hypostomus punctatus Valenciennes (Siluridae)
title Paraoxonase activity in sera from Piaractus mesopotamicus Holmberg (Characidae) and Hypostomus punctatus Valenciennes (Siluridae)
spellingShingle Paraoxonase activity in sera from Piaractus mesopotamicus Holmberg (Characidae) and Hypostomus punctatus Valenciennes (Siluridae)
Bastos,Vera Lucia F. Cunha
Detoxication
fish
organophosphate
serum esterases
title_short Paraoxonase activity in sera from Piaractus mesopotamicus Holmberg (Characidae) and Hypostomus punctatus Valenciennes (Siluridae)
title_full Paraoxonase activity in sera from Piaractus mesopotamicus Holmberg (Characidae) and Hypostomus punctatus Valenciennes (Siluridae)
title_fullStr Paraoxonase activity in sera from Piaractus mesopotamicus Holmberg (Characidae) and Hypostomus punctatus Valenciennes (Siluridae)
title_full_unstemmed Paraoxonase activity in sera from Piaractus mesopotamicus Holmberg (Characidae) and Hypostomus punctatus Valenciennes (Siluridae)
title_sort Paraoxonase activity in sera from Piaractus mesopotamicus Holmberg (Characidae) and Hypostomus punctatus Valenciennes (Siluridae)
author Bastos,Vera Lucia F. Cunha
author_facet Bastos,Vera Lucia F. Cunha
Rossini,Ana
Alves,Marcelo V
Ceccarelli,Paulo S
de Lima,José A. Ferraz
Bastos,Jayme Cunha
author_role author
author2 Rossini,Ana
Alves,Marcelo V
Ceccarelli,Paulo S
de Lima,José A. Ferraz
Bastos,Jayme Cunha
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Bastos,Vera Lucia F. Cunha
Rossini,Ana
Alves,Marcelo V
Ceccarelli,Paulo S
de Lima,José A. Ferraz
Bastos,Jayme Cunha
dc.subject.por.fl_str_mv Detoxication
fish
organophosphate
serum esterases
topic Detoxication
fish
organophosphate
serum esterases
description A paraoxonase activity present in serum of two Brazilian fish species was consistently assayed at pH 8.5 using 7.5 mM paraoxon final concentration. The paraoxonase activity was more activated by 0.5 M NaCl in serum of Piaractus mesopotanricus Holmberg, 1887 (pacu) than in serum of Hypostomus punctatus Valenciennes, 1840 (cascudo). Apparent values of K M were 3.3 x 10-3 M for cascudo and pacu paraoxonase activity in the presence of 0.5 M NaCl. Apparent maximum velocity values calculated in the presence of 0.5 M NaCl were 6.1 and 6.5 nmole/min/mL of serum for cascudo and pacu, respectively. Vmax/K M ratio values of determinations in the presence and absence of 0.5 M NaCl showed that NaCl had a more evident effect on increasing the affinity of serum paraoxonase for paraoxon in pacu serum. Young specimens of pacu showed a marked decreased paraoxonase serum activity when kept in tanks treated with 0.25 ppm methyl-parathion.
publishDate 1998
dc.date.none.fl_str_mv 1998-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-81751998000300011
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-81751998000300011
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0101-81751998000300011
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Zoologia
publisher.none.fl_str_mv Sociedade Brasileira de Zoologia
dc.source.none.fl_str_mv Revista Brasileira de Zoologia v.15 n.3 1998
reponame:Revista Brasileira de Zoologia (Online)
instname:Sociedade Brasileira de Zoologia (SBZ)
instacron:SBZ
instname_str Sociedade Brasileira de Zoologia (SBZ)
instacron_str SBZ
institution SBZ
reponame_str Revista Brasileira de Zoologia (Online)
collection Revista Brasileira de Zoologia (Online)
repository.name.fl_str_mv Revista Brasileira de Zoologia (Online) - Sociedade Brasileira de Zoologia (SBZ)
repository.mail.fl_str_mv ||sbz@bio.ufpr.br
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