Enzyme Detection and Metabolic Process Tracking of Ethanol Fermentation by a Natural Alginate Fermentation Strain

Detalhes bibliográficos
Autor(a) principal: Zhang,Wen
Data de Publicação: 2018
Outros Autores: Ren,Xiaohui, Bao,Liyin
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Archives of Biology and Technology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100201
Resumo: ABSTRACT Alginate is a major component of brown algae, but it cannot be utilized for ethanol fermentation by industrial microorganisms. A natural alginate degrading and ethanol producing strain was obtained in our previous research. However, the research on the ethanol metabolism process of the natural alginate fermentation strain is lacked. In this research, the key enzyme and metabolic process of ethanol fermentation were studied. Three kinds of key enzyme including alginate lyase, pyruvate dehydrogenase and ethanol dehydrogenase were determined. The enzyme activity in the metabolic process was relatively high at 60-96 h which was the most important period during the fermentation. Meanwhile the concentration change of the important substances including soluble sugar, reducing sugar, acidity, pyruvic acid and ethanol were tracked and analyzed. Total soluble sugar and reducing sugar change tendency during the fermentation was similar. In the whole fermentation process, the fermentation broth was acidic. The value of pyruvic acid content reached highest at 72 h. During 48-96 h, the growth of ethanol concentration was very obvious. The alginate metabolic process in natural alginate fermentation strain was to generate extracellular alginate lyase to degrade alginate to produce reducing sugar, and then some intermediate metabolites formed such as pyruvic acid. Finally under the effect of pyruvate dehydrogenase and ethanol dehydrogenase, ethanol was produced.
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spelling Enzyme Detection and Metabolic Process Tracking of Ethanol Fermentation by a Natural Alginate Fermentation StrainAlginate fermentation strainBrown algaeEthanol metabolism processABSTRACT Alginate is a major component of brown algae, but it cannot be utilized for ethanol fermentation by industrial microorganisms. A natural alginate degrading and ethanol producing strain was obtained in our previous research. However, the research on the ethanol metabolism process of the natural alginate fermentation strain is lacked. In this research, the key enzyme and metabolic process of ethanol fermentation were studied. Three kinds of key enzyme including alginate lyase, pyruvate dehydrogenase and ethanol dehydrogenase were determined. The enzyme activity in the metabolic process was relatively high at 60-96 h which was the most important period during the fermentation. Meanwhile the concentration change of the important substances including soluble sugar, reducing sugar, acidity, pyruvic acid and ethanol were tracked and analyzed. Total soluble sugar and reducing sugar change tendency during the fermentation was similar. In the whole fermentation process, the fermentation broth was acidic. The value of pyruvic acid content reached highest at 72 h. During 48-96 h, the growth of ethanol concentration was very obvious. The alginate metabolic process in natural alginate fermentation strain was to generate extracellular alginate lyase to degrade alginate to produce reducing sugar, and then some intermediate metabolites formed such as pyruvic acid. Finally under the effect of pyruvate dehydrogenase and ethanol dehydrogenase, ethanol was produced.Instituto de Tecnologia do Paraná - Tecpar2018-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100201Brazilian Archives of Biology and Technology v.61 2018reponame:Brazilian Archives of Biology and Technologyinstname:Instituto de Tecnologia do Paraná (Tecpar)instacron:TECPAR10.1590/1678-4324-2018160418info:eu-repo/semantics/openAccessZhang,WenRen,XiaohuiBao,Liyineng2018-11-07T00:00:00Zoai:scielo:S1516-89132018000100201Revistahttps://www.scielo.br/j/babt/https://old.scielo.br/oai/scielo-oai.phpbabt@tecpar.br||babt@tecpar.br1678-43241516-8913opendoar:2018-11-07T00:00Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)false
dc.title.none.fl_str_mv Enzyme Detection and Metabolic Process Tracking of Ethanol Fermentation by a Natural Alginate Fermentation Strain
title Enzyme Detection and Metabolic Process Tracking of Ethanol Fermentation by a Natural Alginate Fermentation Strain
spellingShingle Enzyme Detection and Metabolic Process Tracking of Ethanol Fermentation by a Natural Alginate Fermentation Strain
Zhang,Wen
Alginate fermentation strain
Brown algae
Ethanol metabolism process
title_short Enzyme Detection and Metabolic Process Tracking of Ethanol Fermentation by a Natural Alginate Fermentation Strain
title_full Enzyme Detection and Metabolic Process Tracking of Ethanol Fermentation by a Natural Alginate Fermentation Strain
title_fullStr Enzyme Detection and Metabolic Process Tracking of Ethanol Fermentation by a Natural Alginate Fermentation Strain
title_full_unstemmed Enzyme Detection and Metabolic Process Tracking of Ethanol Fermentation by a Natural Alginate Fermentation Strain
title_sort Enzyme Detection and Metabolic Process Tracking of Ethanol Fermentation by a Natural Alginate Fermentation Strain
author Zhang,Wen
author_facet Zhang,Wen
Ren,Xiaohui
Bao,Liyin
author_role author
author2 Ren,Xiaohui
Bao,Liyin
author2_role author
author
dc.contributor.author.fl_str_mv Zhang,Wen
Ren,Xiaohui
Bao,Liyin
dc.subject.por.fl_str_mv Alginate fermentation strain
Brown algae
Ethanol metabolism process
topic Alginate fermentation strain
Brown algae
Ethanol metabolism process
description ABSTRACT Alginate is a major component of brown algae, but it cannot be utilized for ethanol fermentation by industrial microorganisms. A natural alginate degrading and ethanol producing strain was obtained in our previous research. However, the research on the ethanol metabolism process of the natural alginate fermentation strain is lacked. In this research, the key enzyme and metabolic process of ethanol fermentation were studied. Three kinds of key enzyme including alginate lyase, pyruvate dehydrogenase and ethanol dehydrogenase were determined. The enzyme activity in the metabolic process was relatively high at 60-96 h which was the most important period during the fermentation. Meanwhile the concentration change of the important substances including soluble sugar, reducing sugar, acidity, pyruvic acid and ethanol were tracked and analyzed. Total soluble sugar and reducing sugar change tendency during the fermentation was similar. In the whole fermentation process, the fermentation broth was acidic. The value of pyruvic acid content reached highest at 72 h. During 48-96 h, the growth of ethanol concentration was very obvious. The alginate metabolic process in natural alginate fermentation strain was to generate extracellular alginate lyase to degrade alginate to produce reducing sugar, and then some intermediate metabolites formed such as pyruvic acid. Finally under the effect of pyruvate dehydrogenase and ethanol dehydrogenase, ethanol was produced.
publishDate 2018
dc.date.none.fl_str_mv 2018-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
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dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100201
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dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/1678-4324-2018160418
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
dc.source.none.fl_str_mv Brazilian Archives of Biology and Technology v.61 2018
reponame:Brazilian Archives of Biology and Technology
instname:Instituto de Tecnologia do Paraná (Tecpar)
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instname_str Instituto de Tecnologia do Paraná (Tecpar)
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reponame_str Brazilian Archives of Biology and Technology
collection Brazilian Archives of Biology and Technology
repository.name.fl_str_mv Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)
repository.mail.fl_str_mv babt@tecpar.br||babt@tecpar.br
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