In vitro and in vivo nutrient digestibility in sheep of rations with and without residue from the extraction of tamarind pulp

Detalhes bibliográficos
Autor(a) principal: Geron, Luiz Juliano Valério
Data de Publicação: 2015
Outros Autores: Costa, Fabiana Gomes da, Aguiar, Silvia Cristina de, Garcia, Jocilaine, Ribeiro, Matheus Gonçalves, Zeoula, Lúcia Maria, Silva, Ana Paula da, Pereira, Sheslei Ribeiro, Silveira, Roberto Martins
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Semina. Ciências Agrárias (Online)
Texto Completo: https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/20112
Resumo: This study evaluated the digestibility of nutrients by, and parameters associated with, in vitro fermentation using different inocula (sheep ruminal fluid and feces) as well as the in vivo digestibility in sheep that were fed rations with 50% concentrate containing either no (0%) residue from the extraction of tamarind pulp (RETP) or 15% RETP. To determine the in vitro digestibility (IVD) of nutrients, two sheep, weighing 40.38 ± 2.10 kg, were used as inoculum donors. To determine the in vivo digestibility of nutrients, we used four sheep and a 3×2 factorial experimental design, with three methods of digestion of nutrients and two experimental rations (0% and 15% RETP). The variables were subjected to analysis of variance and the variables that showed differences at 5% probability were further analyzed using the Tukey test at 5% significance. The IVD using different inocula did not significantly differ (p>0.05) from the in vivo digestibility in sheep for dry matter (DM), organic matter (OM), and neutral detergent fiber (NDF). The different methods for determining nutrient digestibility did not affect (p>0.05) the digestibility of DM, OM, crude protein (CP), NDF, and acid detergent fiber (ADF) in rations with 0% and 15% RETP. However, the IVD of CP for rations containing 0% and 15% RETP incubated with both inocula was lower (p<0.05) than the CP digestibility in vivo. The in vivo digestibility of ADF for rations containing 0% and 15% RETP was higher (P<0.05) than the IVD using sheep ruminal fluid and feces as inocula. The pH values and concentration of ammonia nitrogen (NH3-N) after in vitro incubation for 24 h and the in vivo assay were not different (p>0.05) for the rations containing 0% and 15% RETP, but the pH and NH3-N of both fermented and rumen contents differed (p<0.05) depending on the inocula used and the in vivo assay. In summary, the digestibility of DM, OM, and NDF can be determined by the in vitro fermentation method using the ruminal fluid or feces of sheep as inocula in rations containing 0% or 15% RETP. However, in vitro fermentation is not a suitable method for the determination of pH and NH3-N concentration.
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spelling In vitro and in vivo nutrient digestibility in sheep of rations with and without residue from the extraction of tamarind pulpDigestibilidade in vitro e in vivo de nutrientes em ovinos para rações contendo ausência ou presença de resíduo da extração da polpa de tamarindoDry matterAmmonia nitrogenCrude proteinpHRumen.Matéria secaNitrogênio amoniacalRúmenProteína brutapH.This study evaluated the digestibility of nutrients by, and parameters associated with, in vitro fermentation using different inocula (sheep ruminal fluid and feces) as well as the in vivo digestibility in sheep that were fed rations with 50% concentrate containing either no (0%) residue from the extraction of tamarind pulp (RETP) or 15% RETP. To determine the in vitro digestibility (IVD) of nutrients, two sheep, weighing 40.38 ± 2.10 kg, were used as inoculum donors. To determine the in vivo digestibility of nutrients, we used four sheep and a 3×2 factorial experimental design, with three methods of digestion of nutrients and two experimental rations (0% and 15% RETP). The variables were subjected to analysis of variance and the variables that showed differences at 5% probability were further analyzed using the Tukey test at 5% significance. The IVD using different inocula did not significantly differ (p>0.05) from the in vivo digestibility in sheep for dry matter (DM), organic matter (OM), and neutral detergent fiber (NDF). The different methods for determining nutrient digestibility did not affect (p>0.05) the digestibility of DM, OM, crude protein (CP), NDF, and acid detergent fiber (ADF) in rations with 0% and 15% RETP. However, the IVD of CP for rations containing 0% and 15% RETP incubated with both inocula was lower (p<0.05) than the CP digestibility in vivo. The in vivo digestibility of ADF for rations containing 0% and 15% RETP was higher (P<0.05) than the IVD using sheep ruminal fluid and feces as inocula. The pH values and concentration of ammonia nitrogen (NH3-N) after in vitro incubation for 24 h and the in vivo assay were not different (p>0.05) for the rations containing 0% and 15% RETP, but the pH and NH3-N of both fermented and rumen contents differed (p<0.05) depending on the inocula used and the in vivo assay. In summary, the digestibility of DM, OM, and NDF can be determined by the in vitro fermentation method using the ruminal fluid or feces of sheep as inocula in rations containing 0% or 15% RETP. However, in vitro fermentation is not a suitable method for the determination of pH and NH3-N concentration.Avaliou-se a digestibilidade de nutrientes e os paramentos da fermentação in vitro com diferentes inóculos (líquido ruminal e fezes de ovinos) e in vivo em ovinos de rações com 50% de concentrado contendo 0% (ausência) ou 15% de resíduo da extração da polpa de tamarindo (REPT). Para a determinação da digestão in vitro dos nutrientes foram utilizados dois ovinos com peso corporal médio de 40,38 kg ± 2,10 kg, como doadores de inóculo e para a determinação da digestibilidade in vivo dos nutrientes foram utilizados quatro ovinos por tratamentos distribuídos em duas rações experimentais com 0% e 15% de REPT. O delineamento experimental foi fatorial 3X2, três métodos de digestão dos nutrientes e duas rações experimentais com 0 e 15% de REPT. As variáveis estudadas foram submetidas à análise de variância e para as variáveis que apresentaram diferença a 5% de probabilidade procedeu-se teste de Tukey a 5% de significância. Os valores de digestibilidade para os diferentes métodos in vitro com diferentes inóculos e in vivo em ovinos para a MS; MO e FDN não apresentaram (P>0,05) diferença entre si. As diferentes metodologias de determinação da digestão dos nutrientes não alteraram (p>0,05) os valores de digestibilidade da MS; MO; PB; FDN e FDA das rações com 0% e 15% do REPT. Entretanto, foi observado que a digestibilidade in vitro da PB das rações com 0% e 15% de REPT incubadas com ambos os inóculos apresentaram (p<0,05) valores menores em relação ao ensaio in vivo com ovinos. Foi observado que a digestibilidade in vivo em ovinos das rações com 0% e 15% de REPT para a FDA apresentaram (p<0,05) valores superiores em relação ao método de digestão in vitro para ambos os inóculos. Os valores de pH e concentração do nitrogênio amoniacal (N-NH3) do conteúdo fermentado após a incubação in vitro e do ensaio in vivo com ovinos não apresentaram (p>0,05) diferença entre as rações experimentais, porém os dados de pH e N-NH3 do conteúdo fermentado ou ruminal apresentaram (p<0,05) alteração para os diferentes inóculos e o ensaio in vivo. Conclui-se que a digestibilidade da MS, MO e FDN pode ser determinada pelo método da fermentação in vitro utilizando como inóculo o líquido ruminal ou as fezes de ovinos para rações com ausência (0%) ou com 15% de REPT. O método de fermentação in vitro não é indicado para a determinação do valor de pH e concentração do nitrogênio amoniacal. UEL2015-12-16info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionpesquisa empírica de campoPesquisa empírica de campoapplication/pdfhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/2011210.5433/1679-0359.2015v36n6Supl2p4507Semina: Ciências Agrárias; Vol. 36 No. 6Supl2 (2015); 4507-4518Semina: Ciências Agrárias; v. 36 n. 6Supl2 (2015); 4507-45181679-03591676-546Xreponame:Semina. Ciências Agrárias (Online)instname:Universidade Estadual de Londrina (UEL)instacron:UELenghttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/20112/17570http://creativecommons.org/licenses/by-nc/4.0info:eu-repo/semantics/openAccessGeron, Luiz Juliano ValérioCosta, Fabiana Gomes daAguiar, Silvia Cristina deGarcia, JocilaineRibeiro, Matheus GonçalvesZeoula, Lúcia MariaSilva, Ana Paula daPereira, Sheslei RibeiroSilveira, Roberto Martins2022-12-02T15:41:49Zoai:ojs.pkp.sfu.ca:article/20112Revistahttp://www.uel.br/revistas/uel/index.php/semagrariasPUBhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/oaisemina.agrarias@uel.br1679-03591676-546Xopendoar:2022-12-02T15:41:49Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)false
dc.title.none.fl_str_mv In vitro and in vivo nutrient digestibility in sheep of rations with and without residue from the extraction of tamarind pulp
Digestibilidade in vitro e in vivo de nutrientes em ovinos para rações contendo ausência ou presença de resíduo da extração da polpa de tamarindo
title In vitro and in vivo nutrient digestibility in sheep of rations with and without residue from the extraction of tamarind pulp
spellingShingle In vitro and in vivo nutrient digestibility in sheep of rations with and without residue from the extraction of tamarind pulp
Geron, Luiz Juliano Valério
Dry matter
Ammonia nitrogen
Crude protein
pH
Rumen.
Matéria seca
Nitrogênio amoniacal
Rúmen
Proteína bruta
pH.
title_short In vitro and in vivo nutrient digestibility in sheep of rations with and without residue from the extraction of tamarind pulp
title_full In vitro and in vivo nutrient digestibility in sheep of rations with and without residue from the extraction of tamarind pulp
title_fullStr In vitro and in vivo nutrient digestibility in sheep of rations with and without residue from the extraction of tamarind pulp
title_full_unstemmed In vitro and in vivo nutrient digestibility in sheep of rations with and without residue from the extraction of tamarind pulp
title_sort In vitro and in vivo nutrient digestibility in sheep of rations with and without residue from the extraction of tamarind pulp
author Geron, Luiz Juliano Valério
author_facet Geron, Luiz Juliano Valério
Costa, Fabiana Gomes da
Aguiar, Silvia Cristina de
Garcia, Jocilaine
Ribeiro, Matheus Gonçalves
Zeoula, Lúcia Maria
Silva, Ana Paula da
Pereira, Sheslei Ribeiro
Silveira, Roberto Martins
author_role author
author2 Costa, Fabiana Gomes da
Aguiar, Silvia Cristina de
Garcia, Jocilaine
Ribeiro, Matheus Gonçalves
Zeoula, Lúcia Maria
Silva, Ana Paula da
Pereira, Sheslei Ribeiro
Silveira, Roberto Martins
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Geron, Luiz Juliano Valério
Costa, Fabiana Gomes da
Aguiar, Silvia Cristina de
Garcia, Jocilaine
Ribeiro, Matheus Gonçalves
Zeoula, Lúcia Maria
Silva, Ana Paula da
Pereira, Sheslei Ribeiro
Silveira, Roberto Martins
dc.subject.por.fl_str_mv Dry matter
Ammonia nitrogen
Crude protein
pH
Rumen.
Matéria seca
Nitrogênio amoniacal
Rúmen
Proteína bruta
pH.
topic Dry matter
Ammonia nitrogen
Crude protein
pH
Rumen.
Matéria seca
Nitrogênio amoniacal
Rúmen
Proteína bruta
pH.
description This study evaluated the digestibility of nutrients by, and parameters associated with, in vitro fermentation using different inocula (sheep ruminal fluid and feces) as well as the in vivo digestibility in sheep that were fed rations with 50% concentrate containing either no (0%) residue from the extraction of tamarind pulp (RETP) or 15% RETP. To determine the in vitro digestibility (IVD) of nutrients, two sheep, weighing 40.38 ± 2.10 kg, were used as inoculum donors. To determine the in vivo digestibility of nutrients, we used four sheep and a 3×2 factorial experimental design, with three methods of digestion of nutrients and two experimental rations (0% and 15% RETP). The variables were subjected to analysis of variance and the variables that showed differences at 5% probability were further analyzed using the Tukey test at 5% significance. The IVD using different inocula did not significantly differ (p>0.05) from the in vivo digestibility in sheep for dry matter (DM), organic matter (OM), and neutral detergent fiber (NDF). The different methods for determining nutrient digestibility did not affect (p>0.05) the digestibility of DM, OM, crude protein (CP), NDF, and acid detergent fiber (ADF) in rations with 0% and 15% RETP. However, the IVD of CP for rations containing 0% and 15% RETP incubated with both inocula was lower (p<0.05) than the CP digestibility in vivo. The in vivo digestibility of ADF for rations containing 0% and 15% RETP was higher (P<0.05) than the IVD using sheep ruminal fluid and feces as inocula. The pH values and concentration of ammonia nitrogen (NH3-N) after in vitro incubation for 24 h and the in vivo assay were not different (p>0.05) for the rations containing 0% and 15% RETP, but the pH and NH3-N of both fermented and rumen contents differed (p<0.05) depending on the inocula used and the in vivo assay. In summary, the digestibility of DM, OM, and NDF can be determined by the in vitro fermentation method using the ruminal fluid or feces of sheep as inocula in rations containing 0% or 15% RETP. However, in vitro fermentation is not a suitable method for the determination of pH and NH3-N concentration.
publishDate 2015
dc.date.none.fl_str_mv 2015-12-16
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
pesquisa empírica de campo
Pesquisa empírica de campo
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/20112
10.5433/1679-0359.2015v36n6Supl2p4507
url https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/20112
identifier_str_mv 10.5433/1679-0359.2015v36n6Supl2p4507
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/20112/17570
dc.rights.driver.fl_str_mv http://creativecommons.org/licenses/by-nc/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv UEL
publisher.none.fl_str_mv UEL
dc.source.none.fl_str_mv Semina: Ciências Agrárias; Vol. 36 No. 6Supl2 (2015); 4507-4518
Semina: Ciências Agrárias; v. 36 n. 6Supl2 (2015); 4507-4518
1679-0359
1676-546X
reponame:Semina. Ciências Agrárias (Online)
instname:Universidade Estadual de Londrina (UEL)
instacron:UEL
instname_str Universidade Estadual de Londrina (UEL)
instacron_str UEL
institution UEL
reponame_str Semina. Ciências Agrárias (Online)
collection Semina. Ciências Agrárias (Online)
repository.name.fl_str_mv Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)
repository.mail.fl_str_mv semina.agrarias@uel.br
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