In vitro and in vivo nutrient digestibility in sheep of rations with and without residue from the extraction of tamarind pulp
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Semina. Ciências Agrárias (Online) |
Texto Completo: | https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/20112 |
Resumo: | This study evaluated the digestibility of nutrients by, and parameters associated with, in vitro fermentation using different inocula (sheep ruminal fluid and feces) as well as the in vivo digestibility in sheep that were fed rations with 50% concentrate containing either no (0%) residue from the extraction of tamarind pulp (RETP) or 15% RETP. To determine the in vitro digestibility (IVD) of nutrients, two sheep, weighing 40.38 ± 2.10 kg, were used as inoculum donors. To determine the in vivo digestibility of nutrients, we used four sheep and a 3×2 factorial experimental design, with three methods of digestion of nutrients and two experimental rations (0% and 15% RETP). The variables were subjected to analysis of variance and the variables that showed differences at 5% probability were further analyzed using the Tukey test at 5% significance. The IVD using different inocula did not significantly differ (p>0.05) from the in vivo digestibility in sheep for dry matter (DM), organic matter (OM), and neutral detergent fiber (NDF). The different methods for determining nutrient digestibility did not affect (p>0.05) the digestibility of DM, OM, crude protein (CP), NDF, and acid detergent fiber (ADF) in rations with 0% and 15% RETP. However, the IVD of CP for rations containing 0% and 15% RETP incubated with both inocula was lower (p<0.05) than the CP digestibility in vivo. The in vivo digestibility of ADF for rations containing 0% and 15% RETP was higher (P<0.05) than the IVD using sheep ruminal fluid and feces as inocula. The pH values and concentration of ammonia nitrogen (NH3-N) after in vitro incubation for 24 h and the in vivo assay were not different (p>0.05) for the rations containing 0% and 15% RETP, but the pH and NH3-N of both fermented and rumen contents differed (p<0.05) depending on the inocula used and the in vivo assay. In summary, the digestibility of DM, OM, and NDF can be determined by the in vitro fermentation method using the ruminal fluid or feces of sheep as inocula in rations containing 0% or 15% RETP. However, in vitro fermentation is not a suitable method for the determination of pH and NH3-N concentration. |
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In vitro and in vivo nutrient digestibility in sheep of rations with and without residue from the extraction of tamarind pulpDigestibilidade in vitro e in vivo de nutrientes em ovinos para rações contendo ausência ou presença de resíduo da extração da polpa de tamarindoDry matterAmmonia nitrogenCrude proteinpHRumen.Matéria secaNitrogênio amoniacalRúmenProteína brutapH.This study evaluated the digestibility of nutrients by, and parameters associated with, in vitro fermentation using different inocula (sheep ruminal fluid and feces) as well as the in vivo digestibility in sheep that were fed rations with 50% concentrate containing either no (0%) residue from the extraction of tamarind pulp (RETP) or 15% RETP. To determine the in vitro digestibility (IVD) of nutrients, two sheep, weighing 40.38 ± 2.10 kg, were used as inoculum donors. To determine the in vivo digestibility of nutrients, we used four sheep and a 3×2 factorial experimental design, with three methods of digestion of nutrients and two experimental rations (0% and 15% RETP). The variables were subjected to analysis of variance and the variables that showed differences at 5% probability were further analyzed using the Tukey test at 5% significance. The IVD using different inocula did not significantly differ (p>0.05) from the in vivo digestibility in sheep for dry matter (DM), organic matter (OM), and neutral detergent fiber (NDF). The different methods for determining nutrient digestibility did not affect (p>0.05) the digestibility of DM, OM, crude protein (CP), NDF, and acid detergent fiber (ADF) in rations with 0% and 15% RETP. However, the IVD of CP for rations containing 0% and 15% RETP incubated with both inocula was lower (p<0.05) than the CP digestibility in vivo. The in vivo digestibility of ADF for rations containing 0% and 15% RETP was higher (P<0.05) than the IVD using sheep ruminal fluid and feces as inocula. The pH values and concentration of ammonia nitrogen (NH3-N) after in vitro incubation for 24 h and the in vivo assay were not different (p>0.05) for the rations containing 0% and 15% RETP, but the pH and NH3-N of both fermented and rumen contents differed (p<0.05) depending on the inocula used and the in vivo assay. In summary, the digestibility of DM, OM, and NDF can be determined by the in vitro fermentation method using the ruminal fluid or feces of sheep as inocula in rations containing 0% or 15% RETP. However, in vitro fermentation is not a suitable method for the determination of pH and NH3-N concentration.Avaliou-se a digestibilidade de nutrientes e os paramentos da fermentação in vitro com diferentes inóculos (líquido ruminal e fezes de ovinos) e in vivo em ovinos de rações com 50% de concentrado contendo 0% (ausência) ou 15% de resíduo da extração da polpa de tamarindo (REPT). Para a determinação da digestão in vitro dos nutrientes foram utilizados dois ovinos com peso corporal médio de 40,38 kg ± 2,10 kg, como doadores de inóculo e para a determinação da digestibilidade in vivo dos nutrientes foram utilizados quatro ovinos por tratamentos distribuídos em duas rações experimentais com 0% e 15% de REPT. O delineamento experimental foi fatorial 3X2, três métodos de digestão dos nutrientes e duas rações experimentais com 0 e 15% de REPT. As variáveis estudadas foram submetidas à análise de variância e para as variáveis que apresentaram diferença a 5% de probabilidade procedeu-se teste de Tukey a 5% de significância. Os valores de digestibilidade para os diferentes métodos in vitro com diferentes inóculos e in vivo em ovinos para a MS; MO e FDN não apresentaram (P>0,05) diferença entre si. As diferentes metodologias de determinação da digestão dos nutrientes não alteraram (p>0,05) os valores de digestibilidade da MS; MO; PB; FDN e FDA das rações com 0% e 15% do REPT. Entretanto, foi observado que a digestibilidade in vitro da PB das rações com 0% e 15% de REPT incubadas com ambos os inóculos apresentaram (p<0,05) valores menores em relação ao ensaio in vivo com ovinos. Foi observado que a digestibilidade in vivo em ovinos das rações com 0% e 15% de REPT para a FDA apresentaram (p<0,05) valores superiores em relação ao método de digestão in vitro para ambos os inóculos. Os valores de pH e concentração do nitrogênio amoniacal (N-NH3) do conteúdo fermentado após a incubação in vitro e do ensaio in vivo com ovinos não apresentaram (p>0,05) diferença entre as rações experimentais, porém os dados de pH e N-NH3 do conteúdo fermentado ou ruminal apresentaram (p<0,05) alteração para os diferentes inóculos e o ensaio in vivo. Conclui-se que a digestibilidade da MS, MO e FDN pode ser determinada pelo método da fermentação in vitro utilizando como inóculo o líquido ruminal ou as fezes de ovinos para rações com ausência (0%) ou com 15% de REPT. O método de fermentação in vitro não é indicado para a determinação do valor de pH e concentração do nitrogênio amoniacal. UEL2015-12-16info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionpesquisa empírica de campoPesquisa empírica de campoapplication/pdfhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/2011210.5433/1679-0359.2015v36n6Supl2p4507Semina: Ciências Agrárias; Vol. 36 No. 6Supl2 (2015); 4507-4518Semina: Ciências Agrárias; v. 36 n. 6Supl2 (2015); 4507-45181679-03591676-546Xreponame:Semina. Ciências Agrárias (Online)instname:Universidade Estadual de Londrina (UEL)instacron:UELenghttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/20112/17570http://creativecommons.org/licenses/by-nc/4.0info:eu-repo/semantics/openAccessGeron, Luiz Juliano ValérioCosta, Fabiana Gomes daAguiar, Silvia Cristina deGarcia, JocilaineRibeiro, Matheus GonçalvesZeoula, Lúcia MariaSilva, Ana Paula daPereira, Sheslei RibeiroSilveira, Roberto Martins2022-12-02T15:41:49Zoai:ojs.pkp.sfu.ca:article/20112Revistahttp://www.uel.br/revistas/uel/index.php/semagrariasPUBhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/oaisemina.agrarias@uel.br1679-03591676-546Xopendoar:2022-12-02T15:41:49Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)false |
dc.title.none.fl_str_mv |
In vitro and in vivo nutrient digestibility in sheep of rations with and without residue from the extraction of tamarind pulp Digestibilidade in vitro e in vivo de nutrientes em ovinos para rações contendo ausência ou presença de resíduo da extração da polpa de tamarindo |
title |
In vitro and in vivo nutrient digestibility in sheep of rations with and without residue from the extraction of tamarind pulp |
spellingShingle |
In vitro and in vivo nutrient digestibility in sheep of rations with and without residue from the extraction of tamarind pulp Geron, Luiz Juliano Valério Dry matter Ammonia nitrogen Crude protein pH Rumen. Matéria seca Nitrogênio amoniacal Rúmen Proteína bruta pH. |
title_short |
In vitro and in vivo nutrient digestibility in sheep of rations with and without residue from the extraction of tamarind pulp |
title_full |
In vitro and in vivo nutrient digestibility in sheep of rations with and without residue from the extraction of tamarind pulp |
title_fullStr |
In vitro and in vivo nutrient digestibility in sheep of rations with and without residue from the extraction of tamarind pulp |
title_full_unstemmed |
In vitro and in vivo nutrient digestibility in sheep of rations with and without residue from the extraction of tamarind pulp |
title_sort |
In vitro and in vivo nutrient digestibility in sheep of rations with and without residue from the extraction of tamarind pulp |
author |
Geron, Luiz Juliano Valério |
author_facet |
Geron, Luiz Juliano Valério Costa, Fabiana Gomes da Aguiar, Silvia Cristina de Garcia, Jocilaine Ribeiro, Matheus Gonçalves Zeoula, Lúcia Maria Silva, Ana Paula da Pereira, Sheslei Ribeiro Silveira, Roberto Martins |
author_role |
author |
author2 |
Costa, Fabiana Gomes da Aguiar, Silvia Cristina de Garcia, Jocilaine Ribeiro, Matheus Gonçalves Zeoula, Lúcia Maria Silva, Ana Paula da Pereira, Sheslei Ribeiro Silveira, Roberto Martins |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
Geron, Luiz Juliano Valério Costa, Fabiana Gomes da Aguiar, Silvia Cristina de Garcia, Jocilaine Ribeiro, Matheus Gonçalves Zeoula, Lúcia Maria Silva, Ana Paula da Pereira, Sheslei Ribeiro Silveira, Roberto Martins |
dc.subject.por.fl_str_mv |
Dry matter Ammonia nitrogen Crude protein pH Rumen. Matéria seca Nitrogênio amoniacal Rúmen Proteína bruta pH. |
topic |
Dry matter Ammonia nitrogen Crude protein pH Rumen. Matéria seca Nitrogênio amoniacal Rúmen Proteína bruta pH. |
description |
This study evaluated the digestibility of nutrients by, and parameters associated with, in vitro fermentation using different inocula (sheep ruminal fluid and feces) as well as the in vivo digestibility in sheep that were fed rations with 50% concentrate containing either no (0%) residue from the extraction of tamarind pulp (RETP) or 15% RETP. To determine the in vitro digestibility (IVD) of nutrients, two sheep, weighing 40.38 ± 2.10 kg, were used as inoculum donors. To determine the in vivo digestibility of nutrients, we used four sheep and a 3×2 factorial experimental design, with three methods of digestion of nutrients and two experimental rations (0% and 15% RETP). The variables were subjected to analysis of variance and the variables that showed differences at 5% probability were further analyzed using the Tukey test at 5% significance. The IVD using different inocula did not significantly differ (p>0.05) from the in vivo digestibility in sheep for dry matter (DM), organic matter (OM), and neutral detergent fiber (NDF). The different methods for determining nutrient digestibility did not affect (p>0.05) the digestibility of DM, OM, crude protein (CP), NDF, and acid detergent fiber (ADF) in rations with 0% and 15% RETP. However, the IVD of CP for rations containing 0% and 15% RETP incubated with both inocula was lower (p<0.05) than the CP digestibility in vivo. The in vivo digestibility of ADF for rations containing 0% and 15% RETP was higher (P<0.05) than the IVD using sheep ruminal fluid and feces as inocula. The pH values and concentration of ammonia nitrogen (NH3-N) after in vitro incubation for 24 h and the in vivo assay were not different (p>0.05) for the rations containing 0% and 15% RETP, but the pH and NH3-N of both fermented and rumen contents differed (p<0.05) depending on the inocula used and the in vivo assay. In summary, the digestibility of DM, OM, and NDF can be determined by the in vitro fermentation method using the ruminal fluid or feces of sheep as inocula in rations containing 0% or 15% RETP. However, in vitro fermentation is not a suitable method for the determination of pH and NH3-N concentration. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-12-16 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion pesquisa empírica de campo Pesquisa empírica de campo |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/20112 10.5433/1679-0359.2015v36n6Supl2p4507 |
url |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/20112 |
identifier_str_mv |
10.5433/1679-0359.2015v36n6Supl2p4507 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/20112/17570 |
dc.rights.driver.fl_str_mv |
http://creativecommons.org/licenses/by-nc/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by-nc/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
UEL |
publisher.none.fl_str_mv |
UEL |
dc.source.none.fl_str_mv |
Semina: Ciências Agrárias; Vol. 36 No. 6Supl2 (2015); 4507-4518 Semina: Ciências Agrárias; v. 36 n. 6Supl2 (2015); 4507-4518 1679-0359 1676-546X reponame:Semina. Ciências Agrárias (Online) instname:Universidade Estadual de Londrina (UEL) instacron:UEL |
instname_str |
Universidade Estadual de Londrina (UEL) |
instacron_str |
UEL |
institution |
UEL |
reponame_str |
Semina. Ciências Agrárias (Online) |
collection |
Semina. Ciências Agrárias (Online) |
repository.name.fl_str_mv |
Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL) |
repository.mail.fl_str_mv |
semina.agrarias@uel.br |
_version_ |
1799306073250725888 |