Seroprevalence fo Anaplasma marginale in dairy cattle and, studies on the dynamics of natural infection of Holstein calves in Southern Brazil
Autor(a) principal: | |
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Data de Publicação: | 2004 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Semina. Ciências Agrárias (Online) |
Texto Completo: | https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/2044 |
Resumo: | Sera of 708 animals (cows, heifers and calves) from 13 dairy herds in the Londrina region of Paraná, Brazil, were tested for antibodies to Anaplasma marginale by a competitive ELISA assay (cELISA). Ten 2 to 20 days old Holstein calves, from one of the 13 herds studied, were monitored during one year. Blood samples from each calf were collected monthly and tick burden counting was performed every fortnight. Percentage of infected erythrocytes was established by Giemsa-stained smears, and sera samples were examined by cELISA to detect antibodies against A. marginale. In the 13 herds, 92.94% of the animals were seropositive to A. marginale, which indicates that Londrina is an area of enzootic stability. Among the three animal categories (cows, heifers and calves), the rates were 98.29%, 96.64% and 81.25%, respectively. Passive transfer of maternal antibodies to calves was demonstrated by cELISA. From ten calves, nine (90%) were seropositive at the first sampling, revealing colostral antibodies anti-A . marginale. These antibodies remained in calves for 2 to 3 months. After this period the calves were infected with ticks, and then all of them were seropositive to Anaplasma. Five 4 to 7 months old calves showed rickettsemia ranging from 0.1% to 3.8%. Two of them were treated with tetracycline. The rickettsemia and clinical signs of anaplasmosis of these calves were coincident with tick burden increase. |
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Semina. Ciências Agrárias (Online) |
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Seroprevalence fo Anaplasma marginale in dairy cattle and, studies on the dynamics of natural infection of Holstein calves in Southern BrazilSoroprevalência de Anaplasma marginale em bovinos leiteiros e estudos sobre a dinâmica da infecção natural em bezerros holandeses no Sul do BrasilAnaplasma marginaleSeroprevalenceDairy cattlecELISATick burden.Anaplasma marginaleSoroprevalênciaBovinos leiteiroscELISAInfestação por carrapato.Sera of 708 animals (cows, heifers and calves) from 13 dairy herds in the Londrina region of Paraná, Brazil, were tested for antibodies to Anaplasma marginale by a competitive ELISA assay (cELISA). Ten 2 to 20 days old Holstein calves, from one of the 13 herds studied, were monitored during one year. Blood samples from each calf were collected monthly and tick burden counting was performed every fortnight. Percentage of infected erythrocytes was established by Giemsa-stained smears, and sera samples were examined by cELISA to detect antibodies against A. marginale. In the 13 herds, 92.94% of the animals were seropositive to A. marginale, which indicates that Londrina is an area of enzootic stability. Among the three animal categories (cows, heifers and calves), the rates were 98.29%, 96.64% and 81.25%, respectively. Passive transfer of maternal antibodies to calves was demonstrated by cELISA. From ten calves, nine (90%) were seropositive at the first sampling, revealing colostral antibodies anti-A . marginale. These antibodies remained in calves for 2 to 3 months. After this period the calves were infected with ticks, and then all of them were seropositive to Anaplasma. Five 4 to 7 months old calves showed rickettsemia ranging from 0.1% to 3.8%. Two of them were treated with tetracycline. The rickettsemia and clinical signs of anaplasmosis of these calves were coincident with tick burden increase.Soros de 708 animais (vacas, novilhas e bezerros) oriundos de 13 propriedades leiteiras da região de Londrina, Estado do Paraná, Brasil, foram testados para a presença de anticorpos contra Anaplasma marginale pelo teste ELISA competitivo (cELISA). Dez bezerros recém nascidos, pertencentes a um dos 13 rebanhos utilizados no levantamento sorológico, foram monitorados durante um ano. De cada bezerro foram colhidas amostras de sangue mensalmente e a cada quinze dias foi realizada a contagem de carrapatos. A porcentagem de eritrócitos parasitados foi determinada pela coloração de Giemsa em esfregaços sanguíneos e as amostras de soros foram submetidas ao cELISA para a detecção de anticorpos contra A. marginale. Dos animais dos rebanhos, 92,94% foram soropositivos para A. marginale indicando que a região de Londrina é uma área de estabilidade enzoótica. Nas três categorias de animais estudadas (vacas, novilhas e bezerros) as taxas de soropositivos foram de 98,29%, 96,64% e 81,25%, respectivamente. Nove dos 10 bezerros (90%) foram positivos pelo cELISA logo após o nascimento, o que demonstra a transferência de anticorpos colostrais. Os níveis de anticorpos colostrais foram detectados até o segundo e o terceiro mês de idade com um decréscimo neste período. Os níveis de anticorpos contra A. marginale voltaram a crescer nos meses seguintes, após os animais se infestarem com o Boophilus microplus, quando todos eram positivos. Cinco bezerros com idades entre quatro e sete meses apresentaram rickettsemias detectáveis pela coloração de Giemsa, variando de 0,1% a 3,8%, sendo que dois destes animais foram tratados com tetraciclina. Estas rickettsemias e os sinais clínicos apresentados por estes bezerros foram coincidentes com o aumento do número de carrapatos sobre os animais.UEL2004-02-28info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/204410.5433/1679-0359.2001v22n2p155Semina: Ciências Agrárias; Vol. 22 No. 2 (2001); 155-159Semina: Ciências Agrárias; v. 22 n. 2 (2001); 155-1591679-03591676-546Xreponame:Semina. Ciências Agrárias (Online)instname:Universidade Estadual de Londrina (UEL)instacron:UELporhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/2044/1754Andrade, Gisele Maria deVidotto, OdilonVidotto, Marilda CarlosYoshihara, EidiKano, Flora S.Amaral, Carlos Henrique S.info:eu-repo/semantics/openAccess2009-05-18T13:38:37Zoai:ojs.pkp.sfu.ca:article/2044Revistahttp://www.uel.br/revistas/uel/index.php/semagrariasPUBhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/oaisemina.agrarias@uel.br1679-03591676-546Xopendoar:2009-05-18T13:38:37Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)false |
dc.title.none.fl_str_mv |
Seroprevalence fo Anaplasma marginale in dairy cattle and, studies on the dynamics of natural infection of Holstein calves in Southern Brazil Soroprevalência de Anaplasma marginale em bovinos leiteiros e estudos sobre a dinâmica da infecção natural em bezerros holandeses no Sul do Brasil |
title |
Seroprevalence fo Anaplasma marginale in dairy cattle and, studies on the dynamics of natural infection of Holstein calves in Southern Brazil |
spellingShingle |
Seroprevalence fo Anaplasma marginale in dairy cattle and, studies on the dynamics of natural infection of Holstein calves in Southern Brazil Andrade, Gisele Maria de Anaplasma marginale Seroprevalence Dairy cattle cELISA Tick burden. Anaplasma marginale Soroprevalência Bovinos leiteiros cELISA Infestação por carrapato. |
title_short |
Seroprevalence fo Anaplasma marginale in dairy cattle and, studies on the dynamics of natural infection of Holstein calves in Southern Brazil |
title_full |
Seroprevalence fo Anaplasma marginale in dairy cattle and, studies on the dynamics of natural infection of Holstein calves in Southern Brazil |
title_fullStr |
Seroprevalence fo Anaplasma marginale in dairy cattle and, studies on the dynamics of natural infection of Holstein calves in Southern Brazil |
title_full_unstemmed |
Seroprevalence fo Anaplasma marginale in dairy cattle and, studies on the dynamics of natural infection of Holstein calves in Southern Brazil |
title_sort |
Seroprevalence fo Anaplasma marginale in dairy cattle and, studies on the dynamics of natural infection of Holstein calves in Southern Brazil |
author |
Andrade, Gisele Maria de |
author_facet |
Andrade, Gisele Maria de Vidotto, Odilon Vidotto, Marilda Carlos Yoshihara, Eidi Kano, Flora S. Amaral, Carlos Henrique S. |
author_role |
author |
author2 |
Vidotto, Odilon Vidotto, Marilda Carlos Yoshihara, Eidi Kano, Flora S. Amaral, Carlos Henrique S. |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Andrade, Gisele Maria de Vidotto, Odilon Vidotto, Marilda Carlos Yoshihara, Eidi Kano, Flora S. Amaral, Carlos Henrique S. |
dc.subject.por.fl_str_mv |
Anaplasma marginale Seroprevalence Dairy cattle cELISA Tick burden. Anaplasma marginale Soroprevalência Bovinos leiteiros cELISA Infestação por carrapato. |
topic |
Anaplasma marginale Seroprevalence Dairy cattle cELISA Tick burden. Anaplasma marginale Soroprevalência Bovinos leiteiros cELISA Infestação por carrapato. |
description |
Sera of 708 animals (cows, heifers and calves) from 13 dairy herds in the Londrina region of Paraná, Brazil, were tested for antibodies to Anaplasma marginale by a competitive ELISA assay (cELISA). Ten 2 to 20 days old Holstein calves, from one of the 13 herds studied, were monitored during one year. Blood samples from each calf were collected monthly and tick burden counting was performed every fortnight. Percentage of infected erythrocytes was established by Giemsa-stained smears, and sera samples were examined by cELISA to detect antibodies against A. marginale. In the 13 herds, 92.94% of the animals were seropositive to A. marginale, which indicates that Londrina is an area of enzootic stability. Among the three animal categories (cows, heifers and calves), the rates were 98.29%, 96.64% and 81.25%, respectively. Passive transfer of maternal antibodies to calves was demonstrated by cELISA. From ten calves, nine (90%) were seropositive at the first sampling, revealing colostral antibodies anti-A . marginale. These antibodies remained in calves for 2 to 3 months. After this period the calves were infected with ticks, and then all of them were seropositive to Anaplasma. Five 4 to 7 months old calves showed rickettsemia ranging from 0.1% to 3.8%. Two of them were treated with tetracycline. The rickettsemia and clinical signs of anaplasmosis of these calves were coincident with tick burden increase. |
publishDate |
2004 |
dc.date.none.fl_str_mv |
2004-02-28 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/2044 10.5433/1679-0359.2001v22n2p155 |
url |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/2044 |
identifier_str_mv |
10.5433/1679-0359.2001v22n2p155 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/2044/1754 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
UEL |
publisher.none.fl_str_mv |
UEL |
dc.source.none.fl_str_mv |
Semina: Ciências Agrárias; Vol. 22 No. 2 (2001); 155-159 Semina: Ciências Agrárias; v. 22 n. 2 (2001); 155-159 1679-0359 1676-546X reponame:Semina. Ciências Agrárias (Online) instname:Universidade Estadual de Londrina (UEL) instacron:UEL |
instname_str |
Universidade Estadual de Londrina (UEL) |
instacron_str |
UEL |
institution |
UEL |
reponame_str |
Semina. Ciências Agrárias (Online) |
collection |
Semina. Ciências Agrárias (Online) |
repository.name.fl_str_mv |
Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL) |
repository.mail.fl_str_mv |
semina.agrarias@uel.br |
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1799306056632893440 |