Experimental infection of BALB/c mice with felid alphaherpesvirus 1
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Semina. Ciências Agrárias (Online) |
Texto Completo: | https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/43252 |
Resumo: | Felid alphaherpesvirus type 1 (FHV-1) is an important cause of respiratory and ocular diseases in cats worldwide. Mice have been widely used to study the pathogenesis of several human and animal viruses, especially herpesviruses. This study aimed to verify whether BALB/c mice are susceptible to FHV-1 infection. The animals were intranasally inoculated with FHV-1 and their clinical signs were observed from 3 days post-infection (dpi). At 10 dpi, the animals were euthanized and the lungs, liver, spleen, and kidneys were collected for histopathological examination and quantitative polymerase chain reaction. The results showed that mice were infected with FHV-1 and reproduced several features of the disease observed in its natural host. Histological lesions and viral DNA were found in all sampled tissues, with a higher frequency of FHV-1 DNA copies detected in the lungs. All mice were seroconverted to FHV-1 at 7 dpi. To our knowledge, this is the first report of experimental infection of BALB/c mice with FHV-1. Our findings demonstrate that this murine model can contribute to our understanding of FHV-1 pathogenesis and may be useful for trials against this virus. |
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Semina. Ciências Agrárias (Online) |
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Experimental infection of BALB/c mice with felid alphaherpesvirus 1Infecção experimental de camundongos BALB/c com alphaherpesvírus felino tipo 1Animal modelCatsExperimental infectionFHV-1qPCR.FHV-1GatosInfecção experimentalModelo animalqPCR.Felid alphaherpesvirus type 1 (FHV-1) is an important cause of respiratory and ocular diseases in cats worldwide. Mice have been widely used to study the pathogenesis of several human and animal viruses, especially herpesviruses. This study aimed to verify whether BALB/c mice are susceptible to FHV-1 infection. The animals were intranasally inoculated with FHV-1 and their clinical signs were observed from 3 days post-infection (dpi). At 10 dpi, the animals were euthanized and the lungs, liver, spleen, and kidneys were collected for histopathological examination and quantitative polymerase chain reaction. The results showed that mice were infected with FHV-1 and reproduced several features of the disease observed in its natural host. Histological lesions and viral DNA were found in all sampled tissues, with a higher frequency of FHV-1 DNA copies detected in the lungs. All mice were seroconverted to FHV-1 at 7 dpi. To our knowledge, this is the first report of experimental infection of BALB/c mice with FHV-1. Our findings demonstrate that this murine model can contribute to our understanding of FHV-1 pathogenesis and may be useful for trials against this virus.O herpesvírus felino tipo 1 (FHV-1) é um importante agente causador de doença respiratória e ocular em gatos em todo o mundo. Camundongos têm sido amplamente utilizados para estudar a patogenia de diversos vírus humanos e animais, especialmente os herpesvírus. O objetivo deste estudo foi verificar se camundongos BALB/c poderiam ser suscetíveis a infecção pelo FHV-1. Os animais foram inoculados com FHV-1 intranasalmente e sinais clínicos foram observados a partir do dia 3 após a infecção (dpi). Após dez dias da inoculação, os animais foram sacrificados e os pulmões, fígado, baço e rins foram coletados para exame histopatológico e PCR quantitativo (qPCR). Os resultados mostraram que os camundongos foram infectados com o FHV-1 e reproduziram várias características da doença que são observadas em seu hospedeiro natural. Lesões histológicas e DNA viral foram detectadas em todos os tecidos amostrados, com maior frequência de DNA do FHV-1 nos pulmões. Todos os camundongos soroconverteram para FHV-1 aos 7 dpi. Para nosso conhecimento, este estudo é o primeiro relato de infecção experimental de camundongos BALB/c com FHV-1. Nossos resultados demonstraram que esse camundongo pode contribuir paro entendimento da patogenia do FHV-1 e pode ser um modelo útil para ensaios contra esse agente.UEL2022-01-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionScientific articleapplication/pdfhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/4325210.5433/1679-0359.2022v43n1p51Semina: Ciências Agrárias; Vol. 43 No. 1 (2022); 51-60Semina: Ciências Agrárias; v. 43 n. 1 (2022); 51-601679-03591676-546Xreponame:Semina. Ciências Agrárias (Online)instname:Universidade Estadual de Londrina (UEL)instacron:UELenghttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/43252/30541Copyright (c) 2021 Semina: Ciências Agráriashttp://creativecommons.org/licenses/by-nc/4.0info:eu-repo/semantics/openAccessSilva, Débora Scopel eCastro, Clarissa Caetano deSilva, Fábio da Silva eGrecco, Fabiane BorelliFonseca, Renata Nobre daFischer, GefersonLima, Marcelo deHübner, Silvia de Oliveira2022-09-26T23:33:10Zoai:ojs.pkp.sfu.ca:article/43252Revistahttp://www.uel.br/revistas/uel/index.php/semagrariasPUBhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/oaisemina.agrarias@uel.br1679-03591676-546Xopendoar:2022-09-26T23:33:10Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)false |
dc.title.none.fl_str_mv |
Experimental infection of BALB/c mice with felid alphaherpesvirus 1 Infecção experimental de camundongos BALB/c com alphaherpesvírus felino tipo 1 |
title |
Experimental infection of BALB/c mice with felid alphaherpesvirus 1 |
spellingShingle |
Experimental infection of BALB/c mice with felid alphaherpesvirus 1 Silva, Débora Scopel e Animal model Cats Experimental infection FHV-1 qPCR. FHV-1 Gatos Infecção experimental Modelo animal qPCR. |
title_short |
Experimental infection of BALB/c mice with felid alphaherpesvirus 1 |
title_full |
Experimental infection of BALB/c mice with felid alphaherpesvirus 1 |
title_fullStr |
Experimental infection of BALB/c mice with felid alphaherpesvirus 1 |
title_full_unstemmed |
Experimental infection of BALB/c mice with felid alphaherpesvirus 1 |
title_sort |
Experimental infection of BALB/c mice with felid alphaherpesvirus 1 |
author |
Silva, Débora Scopel e |
author_facet |
Silva, Débora Scopel e Castro, Clarissa Caetano de Silva, Fábio da Silva e Grecco, Fabiane Borelli Fonseca, Renata Nobre da Fischer, Geferson Lima, Marcelo de Hübner, Silvia de Oliveira |
author_role |
author |
author2 |
Castro, Clarissa Caetano de Silva, Fábio da Silva e Grecco, Fabiane Borelli Fonseca, Renata Nobre da Fischer, Geferson Lima, Marcelo de Hübner, Silvia de Oliveira |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Silva, Débora Scopel e Castro, Clarissa Caetano de Silva, Fábio da Silva e Grecco, Fabiane Borelli Fonseca, Renata Nobre da Fischer, Geferson Lima, Marcelo de Hübner, Silvia de Oliveira |
dc.subject.por.fl_str_mv |
Animal model Cats Experimental infection FHV-1 qPCR. FHV-1 Gatos Infecção experimental Modelo animal qPCR. |
topic |
Animal model Cats Experimental infection FHV-1 qPCR. FHV-1 Gatos Infecção experimental Modelo animal qPCR. |
description |
Felid alphaherpesvirus type 1 (FHV-1) is an important cause of respiratory and ocular diseases in cats worldwide. Mice have been widely used to study the pathogenesis of several human and animal viruses, especially herpesviruses. This study aimed to verify whether BALB/c mice are susceptible to FHV-1 infection. The animals were intranasally inoculated with FHV-1 and their clinical signs were observed from 3 days post-infection (dpi). At 10 dpi, the animals were euthanized and the lungs, liver, spleen, and kidneys were collected for histopathological examination and quantitative polymerase chain reaction. The results showed that mice were infected with FHV-1 and reproduced several features of the disease observed in its natural host. Histological lesions and viral DNA were found in all sampled tissues, with a higher frequency of FHV-1 DNA copies detected in the lungs. All mice were seroconverted to FHV-1 at 7 dpi. To our knowledge, this is the first report of experimental infection of BALB/c mice with FHV-1. Our findings demonstrate that this murine model can contribute to our understanding of FHV-1 pathogenesis and may be useful for trials against this virus. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-01-10 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Scientific article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/43252 10.5433/1679-0359.2022v43n1p51 |
url |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/43252 |
identifier_str_mv |
10.5433/1679-0359.2022v43n1p51 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/43252/30541 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2021 Semina: Ciências Agrárias http://creativecommons.org/licenses/by-nc/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2021 Semina: Ciências Agrárias http://creativecommons.org/licenses/by-nc/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
UEL |
publisher.none.fl_str_mv |
UEL |
dc.source.none.fl_str_mv |
Semina: Ciências Agrárias; Vol. 43 No. 1 (2022); 51-60 Semina: Ciências Agrárias; v. 43 n. 1 (2022); 51-60 1679-0359 1676-546X reponame:Semina. Ciências Agrárias (Online) instname:Universidade Estadual de Londrina (UEL) instacron:UEL |
instname_str |
Universidade Estadual de Londrina (UEL) |
instacron_str |
UEL |
institution |
UEL |
reponame_str |
Semina. Ciências Agrárias (Online) |
collection |
Semina. Ciências Agrárias (Online) |
repository.name.fl_str_mv |
Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL) |
repository.mail.fl_str_mv |
semina.agrarias@uel.br |
_version_ |
1750315433148284928 |