Utilization of horse plasma in coagulase test for Staphylococci isolated from raw milk

Detalhes bibliográficos
Autor(a) principal: Silva, Luiz Henrique Cabral da
Data de Publicação: 2005
Outros Autores: Monteiro, Alexandre Amorim, Beloti, Vanerli, Barros, Márcia de Aguiar Ferreira, Nero, Luís Augusto, Santana, Elsa Helena Walter de, Moraes, Luciane Bilia de
Tipo de documento: Artigo
Idioma: por
Título da fonte: Semina. Ciências Agrárias (Online)
Texto Completo: https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/2314
Resumo: Staphylococci are frequently related to food poisoning outbreaks that are caused by their enterotoxins. The enterotoxins production is highly correlated to coagulase production, which one is detected in identification of pathogenic strains. Usually the coagulase test is conducted using lyophilized rabbit plasma, or even fresh rabbit blood, separated by sedimentation. The lyophilized plasma has high costs and the fresh material is obtained in small volumes from the animals, what made it unpractical. The objective of this work was evaluating the utilization of an alternative material to rabbit plasma, in this case the horse plasma, in coagulase test for staphylococci isolated from raw milk. So, 410 typical and atypical staphylococci colonies were tested for coagulase production using both plasmas. The results showed 284 (69.26%) positives for coagulase production and 126 (30.73%) negatives, with 100% of concordance between both plasmas. These results indicate the viability in using horse plasma in coagulase test for staphylococci isolated from raw milk, and complementary studies are necessary with strains from different types of food.
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spelling Utilization of horse plasma in coagulase test for Staphylococci isolated from raw milkUtilização de plasma de cavalo no teste de coagulase em Estafilococos isolados de leite cruMilkStaphylococciCoagulase testMethodology.LeiteEstafilococosTeste de coagulaseMetodologia.Staphylococci are frequently related to food poisoning outbreaks that are caused by their enterotoxins. The enterotoxins production is highly correlated to coagulase production, which one is detected in identification of pathogenic strains. Usually the coagulase test is conducted using lyophilized rabbit plasma, or even fresh rabbit blood, separated by sedimentation. The lyophilized plasma has high costs and the fresh material is obtained in small volumes from the animals, what made it unpractical. The objective of this work was evaluating the utilization of an alternative material to rabbit plasma, in this case the horse plasma, in coagulase test for staphylococci isolated from raw milk. So, 410 typical and atypical staphylococci colonies were tested for coagulase production using both plasmas. The results showed 284 (69.26%) positives for coagulase production and 126 (30.73%) negatives, with 100% of concordance between both plasmas. These results indicate the viability in using horse plasma in coagulase test for staphylococci isolated from raw milk, and complementary studies are necessary with strains from different types of food.Estafilococos têm grande importância na indústria de alimentos por serem associados a surtos de intoxicação alimentar, pela ação de suas enterotoxinas. A produção dessas substâncias tem alta correlação com a produção da enzima coagulase, que é detectada na identificação de cepas potencialmente patogênicas. Esse teste é tradicionalmente realizado com plasma de coelho liofilizado ou pela sua separação de sangue coletado de coelhos. O plasma liofilizado tem alto custo e a punção cardíaca, além de ser bastante cruenta, permite a obtenção apenas de pequenos volumes de material. Este trabalho tem como objetivo verificar a viabilidade da utilização de um material alternativo ao plasma de coelho, neste caso o plasma de cavalo, no teste de coagulase para estafilococos isolados de leite cru. Assim, 410 colônias típicas e atípicas de estafilococos isoladas de leite cru foram submetidas ao teste de coagulase utilizandose os dois plasmas. Os resultados identificaram 284 (69,26%) colônias coagulase positivas e 126 (30,73%) coagulase negativas, com concordância de 100% entre os dois plasmas. Os resultados obtidos indicam que a utilização do plasma de cavalo pode ser uma alternativa viável na pesquisa de estafilococos coagulase positivos em leite cru.UEL2005-06-30info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/231410.5433/1679-0359.2005v26n3p381Semina: Ciências Agrárias; Vol. 26 No. 3 (2005); 381-386Semina: Ciências Agrárias; v. 26 n. 3 (2005); 381-3861679-03591676-546Xreponame:Semina. Ciências Agrárias (Online)instname:Universidade Estadual de Londrina (UEL)instacron:UELporhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/2314/1992Silva, Luiz Henrique Cabral daMonteiro, Alexandre AmorimBeloti, VanerliBarros, Márcia de Aguiar FerreiraNero, Luís AugustoSantana, Elsa Helena Walter deMoraes, Luciane Bilia deinfo:eu-repo/semantics/openAccess2009-10-08T13:43:59Zoai:ojs.pkp.sfu.ca:article/2314Revistahttp://www.uel.br/revistas/uel/index.php/semagrariasPUBhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/oaisemina.agrarias@uel.br1679-03591676-546Xopendoar:2009-10-08T13:43:59Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)false
dc.title.none.fl_str_mv Utilization of horse plasma in coagulase test for Staphylococci isolated from raw milk
Utilização de plasma de cavalo no teste de coagulase em Estafilococos isolados de leite cru
title Utilization of horse plasma in coagulase test for Staphylococci isolated from raw milk
spellingShingle Utilization of horse plasma in coagulase test for Staphylococci isolated from raw milk
Silva, Luiz Henrique Cabral da
Milk
Staphylococci
Coagulase test
Methodology.
Leite
Estafilococos
Teste de coagulase
Metodologia.
title_short Utilization of horse plasma in coagulase test for Staphylococci isolated from raw milk
title_full Utilization of horse plasma in coagulase test for Staphylococci isolated from raw milk
title_fullStr Utilization of horse plasma in coagulase test for Staphylococci isolated from raw milk
title_full_unstemmed Utilization of horse plasma in coagulase test for Staphylococci isolated from raw milk
title_sort Utilization of horse plasma in coagulase test for Staphylococci isolated from raw milk
author Silva, Luiz Henrique Cabral da
author_facet Silva, Luiz Henrique Cabral da
Monteiro, Alexandre Amorim
Beloti, Vanerli
Barros, Márcia de Aguiar Ferreira
Nero, Luís Augusto
Santana, Elsa Helena Walter de
Moraes, Luciane Bilia de
author_role author
author2 Monteiro, Alexandre Amorim
Beloti, Vanerli
Barros, Márcia de Aguiar Ferreira
Nero, Luís Augusto
Santana, Elsa Helena Walter de
Moraes, Luciane Bilia de
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Silva, Luiz Henrique Cabral da
Monteiro, Alexandre Amorim
Beloti, Vanerli
Barros, Márcia de Aguiar Ferreira
Nero, Luís Augusto
Santana, Elsa Helena Walter de
Moraes, Luciane Bilia de
dc.subject.por.fl_str_mv Milk
Staphylococci
Coagulase test
Methodology.
Leite
Estafilococos
Teste de coagulase
Metodologia.
topic Milk
Staphylococci
Coagulase test
Methodology.
Leite
Estafilococos
Teste de coagulase
Metodologia.
description Staphylococci are frequently related to food poisoning outbreaks that are caused by their enterotoxins. The enterotoxins production is highly correlated to coagulase production, which one is detected in identification of pathogenic strains. Usually the coagulase test is conducted using lyophilized rabbit plasma, or even fresh rabbit blood, separated by sedimentation. The lyophilized plasma has high costs and the fresh material is obtained in small volumes from the animals, what made it unpractical. The objective of this work was evaluating the utilization of an alternative material to rabbit plasma, in this case the horse plasma, in coagulase test for staphylococci isolated from raw milk. So, 410 typical and atypical staphylococci colonies were tested for coagulase production using both plasmas. The results showed 284 (69.26%) positives for coagulase production and 126 (30.73%) negatives, with 100% of concordance between both plasmas. These results indicate the viability in using horse plasma in coagulase test for staphylococci isolated from raw milk, and complementary studies are necessary with strains from different types of food.
publishDate 2005
dc.date.none.fl_str_mv 2005-06-30
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/2314
10.5433/1679-0359.2005v26n3p381
url https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/2314
identifier_str_mv 10.5433/1679-0359.2005v26n3p381
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/2314/1992
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv UEL
publisher.none.fl_str_mv UEL
dc.source.none.fl_str_mv Semina: Ciências Agrárias; Vol. 26 No. 3 (2005); 381-386
Semina: Ciências Agrárias; v. 26 n. 3 (2005); 381-386
1679-0359
1676-546X
reponame:Semina. Ciências Agrárias (Online)
instname:Universidade Estadual de Londrina (UEL)
instacron:UEL
instname_str Universidade Estadual de Londrina (UEL)
instacron_str UEL
institution UEL
reponame_str Semina. Ciências Agrárias (Online)
collection Semina. Ciências Agrárias (Online)
repository.name.fl_str_mv Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)
repository.mail.fl_str_mv semina.agrarias@uel.br
_version_ 1750315406263844864

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