Selenite biotransformation during brewing. Evaluation by HPLC–ICP-MS

Detalhes bibliográficos
Autor(a) principal: Martínez, Maria Sánchez
Data de Publicação: 2012
Outros Autores: Silva, Erik Galvão Paranhos da, Corona, Teresa Pérez, Cámara, Carmen, Ferreira, Sergio Luis Costa, Madrid, Yolanda
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFBA
Texto Completo: http://repositorio.ufba.br/ri/handle/ri/15705
Resumo: Texto completo: acesso restrito. p. 272–276
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spelling Martínez, Maria SánchezSilva, Erik Galvão Paranhos daCorona, Teresa PérezCámara, CarmenFerreira, Sergio Luis CostaMadrid, YolandaMartínez, Maria SánchezSilva, Erik Galvão Paranhos daCorona, Teresa PérezCámara, CarmenFerreira, Sergio Luis CostaMadrid, Yolanda2014-08-26T13:03:49Z20120039-9140http://repositorio.ufba.br/ri/handle/ri/15705v. 88, n. 15Texto completo: acesso restrito. p. 272–276Yeast (Saccharomyces cerevisiae) and lactic bacteria have shown their ability to accumulate and transform inorganic selenium into organo Se compounds. The objective of this work was to evaluate selenium biotransformation during brewing by using S. cerevisiae and Saccharomyces uvarum for Ale and Lager fermentation, respectively. Se-enriched beer was produced by the addition of sodium selenite (0, 0.2, 1.0, 2.0, 10.0, 20.0 μg Se mL−1, respectively) to the fermentation media composed of yeast, malt extract and water. The alcoholic fermentation process was not affected by the presence of selenium regardless of the type of Saccharomyces being used. The percentage of selenium incorporated into beer, added between 1.0 and 10 μg mL−1 was 55–60% of the selenium initially present. Se-compounds in post-fermentation (beer and yeast) products were investigated by using an analytical methodology based on HPLC–ICP-MS. For this purpose, several sample treatments, including ultrasonic-assisted enzymatic hydrolysis, in conjunction with different separation mechanisms like dialysis and anion exchange HPLC chromatography were applied for unambiguously identifying Se-species that produce during brewing. Selenomethionine was the main selenium compound identified in beer and yeast, being this species in the only case of the former not associated to peptides or proteins.Submitted by Edileide Reis (leyde-landy@hotmail.com) on 2014-08-26T13:03:49Z No. of bitstreams: 1 Maria Sánchez Martínez.pdf: 259346 bytes, checksum: fa7bcb9a92ae9ffd3eeab812db211e1c (MD5)Made available in DSpace on 2014-08-26T13:03:49Z (GMT). No. of bitstreams: 1 Maria Sánchez Martínez.pdf: 259346 bytes, checksum: fa7bcb9a92ae9ffd3eeab812db211e1c (MD5) Previous issue date: 2012http://dx.doi.org/ 10.1016/j.talanta.2011.10.041reponame:Repositório Institucional da UFBAinstname:Universidade Federal da Bahia (UFBA)instacron:UFBABiotransformationSeleniumBeerHPLC–ICP-MSSelenite biotransformation during brewing. 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dc.title.pt_BR.fl_str_mv Selenite biotransformation during brewing. Evaluation by HPLC–ICP-MS
dc.title.alternative.pt_BR.fl_str_mv Talanta
title Selenite biotransformation during brewing. Evaluation by HPLC–ICP-MS
spellingShingle Selenite biotransformation during brewing. Evaluation by HPLC–ICP-MS
Martínez, Maria Sánchez
Biotransformation
Selenium
Beer
HPLC–ICP-MS
title_short Selenite biotransformation during brewing. Evaluation by HPLC–ICP-MS
title_full Selenite biotransformation during brewing. Evaluation by HPLC–ICP-MS
title_fullStr Selenite biotransformation during brewing. Evaluation by HPLC–ICP-MS
title_full_unstemmed Selenite biotransformation during brewing. Evaluation by HPLC–ICP-MS
title_sort Selenite biotransformation during brewing. Evaluation by HPLC–ICP-MS
author Martínez, Maria Sánchez
author_facet Martínez, Maria Sánchez
Silva, Erik Galvão Paranhos da
Corona, Teresa Pérez
Cámara, Carmen
Ferreira, Sergio Luis Costa
Madrid, Yolanda
author_role author
author2 Silva, Erik Galvão Paranhos da
Corona, Teresa Pérez
Cámara, Carmen
Ferreira, Sergio Luis Costa
Madrid, Yolanda
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Martínez, Maria Sánchez
Silva, Erik Galvão Paranhos da
Corona, Teresa Pérez
Cámara, Carmen
Ferreira, Sergio Luis Costa
Madrid, Yolanda
Martínez, Maria Sánchez
Silva, Erik Galvão Paranhos da
Corona, Teresa Pérez
Cámara, Carmen
Ferreira, Sergio Luis Costa
Madrid, Yolanda
dc.subject.por.fl_str_mv Biotransformation
Selenium
Beer
HPLC–ICP-MS
topic Biotransformation
Selenium
Beer
HPLC–ICP-MS
description Texto completo: acesso restrito. p. 272–276
publishDate 2012
dc.date.issued.fl_str_mv 2012
dc.date.accessioned.fl_str_mv 2014-08-26T13:03:49Z
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dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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dc.identifier.uri.fl_str_mv http://repositorio.ufba.br/ri/handle/ri/15705
dc.identifier.issn.none.fl_str_mv 0039-9140
dc.identifier.number.pt_BR.fl_str_mv v. 88, n. 15
identifier_str_mv 0039-9140
v. 88, n. 15
url http://repositorio.ufba.br/ri/handle/ri/15705
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.source.pt_BR.fl_str_mv http://dx.doi.org/ 10.1016/j.talanta.2011.10.041
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