Human T cell epitope mapping of the Schistosoma mansoni 14-kDa fatty acid-binding protein using cells from patients living in areas endemic for schistosomiasis

Detalhes bibliográficos
Autor(a) principal: Fonseca, Cristina Toscano
Data de Publicação: 2005
Outros Autores: Cunha Neto, Edécio, Goldberg, Anna Carla Renata Krepel, Kalil, Jorge, Jesus, Amélia Maria Ribeiro de, Carvalho Filho, Edgar Marcelino de, Oliveira, Rodrigo Correa, Oliveira, Sérgio Costa
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFBA
Texto Completo: http://www.repositorio.ufba.br/ri/handle/ri/6607
Resumo: Trabalho completo: acesso restrito, p. 204–212
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spelling Fonseca, Cristina ToscanoCunha Neto, EdécioGoldberg, Anna Carla Renata KrepelKalil, JorgeJesus, Amélia Maria Ribeiro deCarvalho Filho, Edgar Marcelino deOliveira, Rodrigo CorreaOliveira, Sérgio CostaFonseca, Cristina ToscanoCunha Neto, EdécioGoldberg, Anna Carla Renata KrepelKalil, JorgeJesus, Amélia Maria Ribeiro deCarvalho Filho, Edgar Marcelino deOliveira, Rodrigo CorreaOliveira, Sérgio Costa2012-08-20T19:09:29Z2005-021286-4579http://www.repositorio.ufba.br/ri/handle/ri/6607v. 7, n. 2Trabalho completo: acesso restrito, p. 204–212he development of a defined anti-schistosomiasis vaccine would contribute to the current control strategy mainly because immunization provides long-lasting immunity to the disease. Sm14, one of the six Schistosoma mansoni antigens selected by WHO as a candidate to compose a subunit vaccine against schistosomiasis, has been associated with resistance to S. mansoni infection in human beings and is able to induce protection in the murine model. To identify human T cell epitopes in Sm14, we used the TEPITOPE algorithm to select peptides that would most likely bind to several HLA-DR molecules. In this study, three Sm14 epitopes were selected and produced as synthetic peptides. Human T cell responses from schistosomiasis patients living in endemic areas in Brazil were determined by proliferation assay and IL-5 and IFN-γ measurements. Differential peptide recognition and cytokine production in response to Sm14 epitopes were observed in individuals resistant to S. mansoni infection versus susceptible individuals. Sm14(32-48) and Sm14(53-69) peptides were preferentially recognized by peripheral blood mononuclear cells (PBMCs) of S. mansoni-resistant individuals, and Sm14(53-69) induced significant production of IFN-γ. Additionally, Sm14(32-48) and Sm14(53-69) were “promiscuous” peptides, since they were able to induce cellular immune responses in individuals carrying 10 and 8, respectively, of the 11 HLA-DR molecules expressed in the studied population. Among Sm14 synthetic peptides tested in this study, we identified Sm14(32-48) and Sm14(53-69) as promising candidates to compose an anti-schistosomiasis vaccine, since they seem to be related to resistance to human schistosomiasis.Submitted by Bruna Lessa (lessbruna@gmail.com) on 2012-08-20T19:09:29Z No. of bitstreams: 1 (172).pdf: 354283 bytes, checksum: 782806db1c3215c834afd72dc34157d5 (MD5)Made available in DSpace on 2012-08-20T19:09:29Z (GMT). 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dc.title.pt_BR.fl_str_mv Human T cell epitope mapping of the Schistosoma mansoni 14-kDa fatty acid-binding protein using cells from patients living in areas endemic for schistosomiasis
dc.title.alternative.pt_BR.fl_str_mv Microbes and Infection
title Human T cell epitope mapping of the Schistosoma mansoni 14-kDa fatty acid-binding protein using cells from patients living in areas endemic for schistosomiasis
spellingShingle Human T cell epitope mapping of the Schistosoma mansoni 14-kDa fatty acid-binding protein using cells from patients living in areas endemic for schistosomiasis
Fonseca, Cristina Toscano
Vaccine
Human T cells
Synthetic peptides
Schistosoma mansoni
Sm14
title_short Human T cell epitope mapping of the Schistosoma mansoni 14-kDa fatty acid-binding protein using cells from patients living in areas endemic for schistosomiasis
title_full Human T cell epitope mapping of the Schistosoma mansoni 14-kDa fatty acid-binding protein using cells from patients living in areas endemic for schistosomiasis
title_fullStr Human T cell epitope mapping of the Schistosoma mansoni 14-kDa fatty acid-binding protein using cells from patients living in areas endemic for schistosomiasis
title_full_unstemmed Human T cell epitope mapping of the Schistosoma mansoni 14-kDa fatty acid-binding protein using cells from patients living in areas endemic for schistosomiasis
title_sort Human T cell epitope mapping of the Schistosoma mansoni 14-kDa fatty acid-binding protein using cells from patients living in areas endemic for schistosomiasis
author Fonseca, Cristina Toscano
author_facet Fonseca, Cristina Toscano
Cunha Neto, Edécio
Goldberg, Anna Carla Renata Krepel
Kalil, Jorge
Jesus, Amélia Maria Ribeiro de
Carvalho Filho, Edgar Marcelino de
Oliveira, Rodrigo Correa
Oliveira, Sérgio Costa
author_role author
author2 Cunha Neto, Edécio
Goldberg, Anna Carla Renata Krepel
Kalil, Jorge
Jesus, Amélia Maria Ribeiro de
Carvalho Filho, Edgar Marcelino de
Oliveira, Rodrigo Correa
Oliveira, Sérgio Costa
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Fonseca, Cristina Toscano
Cunha Neto, Edécio
Goldberg, Anna Carla Renata Krepel
Kalil, Jorge
Jesus, Amélia Maria Ribeiro de
Carvalho Filho, Edgar Marcelino de
Oliveira, Rodrigo Correa
Oliveira, Sérgio Costa
Fonseca, Cristina Toscano
Cunha Neto, Edécio
Goldberg, Anna Carla Renata Krepel
Kalil, Jorge
Jesus, Amélia Maria Ribeiro de
Carvalho Filho, Edgar Marcelino de
Oliveira, Rodrigo Correa
Oliveira, Sérgio Costa
dc.subject.por.fl_str_mv Vaccine
Human T cells
Synthetic peptides
Schistosoma mansoni
Sm14
topic Vaccine
Human T cells
Synthetic peptides
Schistosoma mansoni
Sm14
description Trabalho completo: acesso restrito, p. 204–212
publishDate 2005
dc.date.issued.fl_str_mv 2005-02
dc.date.accessioned.fl_str_mv 2012-08-20T19:09:29Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.repositorio.ufba.br/ri/handle/ri/6607
dc.identifier.issn.none.fl_str_mv 1286-4579
dc.identifier.number.pt_BR.fl_str_mv v. 7, n. 2
identifier_str_mv 1286-4579
v. 7, n. 2
url http://www.repositorio.ufba.br/ri/handle/ri/6607
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.pt_BR.fl_str_mv http://dx.doi.org/10.1016/j.micinf.2004.10.012
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFBA
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