Comparison of six methods for the extraction of lipids from serum in terms of effectiveness and protein preservation

Detalhes bibliográficos
Autor(a) principal: Ferraz, T. P. L.
Data de Publicação: 2004
Outros Autores: Fiúza, M. C., Santos, M. L. A. dos, Carvalho, Lain Carlos Pontes de, Soares, N. M.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFBA
Texto Completo: http://www.repositorio.ufba.br/ri/handle/ri/7728
Resumo: Texto completo: acesso restrito. p. 187–193
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spelling Ferraz, T. P. L.Fiúza, M. C.Santos, M. L. A. dosCarvalho, Lain Carlos Pontes deSoares, N. M.Ferraz, T. P. L.Fiúza, M. C.Santos, M. L. A. dosCarvalho, Lain Carlos Pontes deSoares, N. M.2013-01-03T18:22:00Z20040165-022Xhttp://www.repositorio.ufba.br/ri/handle/ri/7728v. 58, n. 3Texto completo: acesso restrito. p. 187–193The present work compares six biochemical methods for extraction of lipids from human serum.Although some organic solvents were good lipid extractors, they precipitated most of the total proteins and albumin. On the other hand, methodologies using Triton X-114 and silica were efficient for extraction of lipids, while sparing the protein fraction.Submitted by JURANDI DE SOUZA SILVA (jssufba@hotmail.com) on 2013-01-03T18:22:00Z No. of bitstreams: 1 1-s2.0-S0165022X03002057-main.pdf: 206713 bytes, checksum: d6f3ae6ffe05064843dd31f40a194e93 (MD5)Made available in DSpace on 2013-01-03T18:22:00Z (GMT). No. of bitstreams: 1 1-s2.0-S0165022X03002057-main.pdf: 206713 bytes, checksum: d6f3ae6ffe05064843dd31f40a194e93 (MD5) Previous issue date: 2004Elservierhttp://dx.doi.org/10.1016/j.jbbm.2003.10.008reponame:Repositório Institucional da UFBAinstname:Universidade Federal da Bahia (UFBA)instacron:UFBALipid extractionProteinsSilicaDetergentComparison of six methods for the extraction of lipids from serum in terms of effectiveness and protein preservationJournal of Biochemical and Biophysical Methodsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article10000-01-01enginfo:eu-repo/semantics/openAccessORIGINAL1-s2.0-S0165022X03002057-main.pdf1-s2.0-S0165022X03002057-main.pdfapplication/pdf206713https://repositorio.ufba.br/bitstream/ri/7728/1/1-s2.0-S0165022X03002057-main.pdfd6f3ae6ffe05064843dd31f40a194e93MD51LICENSElicense.txtlicense.txttext/plain1762https://repositorio.ufba.br/bitstream/ri/7728/2/license.txt1b89a9a0548218172d7c829f87a0eab9MD52TEXT1-s2.0-S0165022X03002057-main.pdf.txt1-s2.0-S0165022X03002057-main.pdf.txtExtracted texttext/plain15099https://repositorio.ufba.br/bitstream/ri/7728/3/1-s2.0-S0165022X03002057-main.pdf.txte8cc5f34e2a0329ed4b1538aafe3ae5bMD53ri/77282022-07-05 14:03:22.305oai:repositorio.ufba.br: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Repositório InstitucionalPUBhttp://192.188.11.11:8080/oai/requestopendoar:19322022-07-05T17:03:22Repositório Institucional da UFBA - Universidade Federal da Bahia (UFBA)false
dc.title.pt_BR.fl_str_mv Comparison of six methods for the extraction of lipids from serum in terms of effectiveness and protein preservation
dc.title.alternative.pt_BR.fl_str_mv Journal of Biochemical and Biophysical Methods
title Comparison of six methods for the extraction of lipids from serum in terms of effectiveness and protein preservation
spellingShingle Comparison of six methods for the extraction of lipids from serum in terms of effectiveness and protein preservation
Ferraz, T. P. L.
Lipid extraction
Proteins
Silica
Detergent
title_short Comparison of six methods for the extraction of lipids from serum in terms of effectiveness and protein preservation
title_full Comparison of six methods for the extraction of lipids from serum in terms of effectiveness and protein preservation
title_fullStr Comparison of six methods for the extraction of lipids from serum in terms of effectiveness and protein preservation
title_full_unstemmed Comparison of six methods for the extraction of lipids from serum in terms of effectiveness and protein preservation
title_sort Comparison of six methods for the extraction of lipids from serum in terms of effectiveness and protein preservation
author Ferraz, T. P. L.
author_facet Ferraz, T. P. L.
Fiúza, M. C.
Santos, M. L. A. dos
Carvalho, Lain Carlos Pontes de
Soares, N. M.
author_role author
author2 Fiúza, M. C.
Santos, M. L. A. dos
Carvalho, Lain Carlos Pontes de
Soares, N. M.
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Ferraz, T. P. L.
Fiúza, M. C.
Santos, M. L. A. dos
Carvalho, Lain Carlos Pontes de
Soares, N. M.
Ferraz, T. P. L.
Fiúza, M. C.
Santos, M. L. A. dos
Carvalho, Lain Carlos Pontes de
Soares, N. M.
dc.subject.por.fl_str_mv Lipid extraction
Proteins
Silica
Detergent
topic Lipid extraction
Proteins
Silica
Detergent
description Texto completo: acesso restrito. p. 187–193
publishDate 2004
dc.date.issued.fl_str_mv 2004
dc.date.accessioned.fl_str_mv 2013-01-03T18:22:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.repositorio.ufba.br/ri/handle/ri/7728
dc.identifier.issn.none.fl_str_mv 0165-022X
dc.identifier.number.pt_BR.fl_str_mv v. 58, n. 3
identifier_str_mv 0165-022X
v. 58, n. 3
url http://www.repositorio.ufba.br/ri/handle/ri/7728
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elservier
publisher.none.fl_str_mv Elservier
dc.source.pt_BR.fl_str_mv http://dx.doi.org/10.1016/j.jbbm.2003.10.008
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFBA
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instacron_str UFBA
institution UFBA
reponame_str Repositório Institucional da UFBA
collection Repositório Institucional da UFBA
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