Comparison of six methods for the extraction of lipids from serum in terms of effectiveness and protein preservation
Main Author: | |
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Publication Date: | 2004 |
Other Authors: | , , , |
Format: | Article |
Language: | eng |
Source: | Repositório Institucional da UFBA |
Download full: | http://www.repositorio.ufba.br/ri/handle/ri/7728 |
Summary: | Texto completo: acesso restrito. p. 187–193 |
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Ferraz, T. P. L.Fiúza, M. C.Santos, M. L. A. dosCarvalho, Lain Carlos Pontes deSoares, N. M.Ferraz, T. P. L.Fiúza, M. C.Santos, M. L. A. dosCarvalho, Lain Carlos Pontes deSoares, N. M.2013-01-03T18:22:00Z20040165-022Xhttp://www.repositorio.ufba.br/ri/handle/ri/7728v. 58, n. 3Texto completo: acesso restrito. p. 187–193The present work compares six biochemical methods for extraction of lipids from human serum.Although some organic solvents were good lipid extractors, they precipitated most of the total proteins and albumin. On the other hand, methodologies using Triton X-114 and silica were efficient for extraction of lipids, while sparing the protein fraction.Submitted by JURANDI DE SOUZA SILVA (jssufba@hotmail.com) on 2013-01-03T18:22:00Z No. of bitstreams: 1 1-s2.0-S0165022X03002057-main.pdf: 206713 bytes, checksum: d6f3ae6ffe05064843dd31f40a194e93 (MD5)Made available in DSpace on 2013-01-03T18:22:00Z (GMT). No. of bitstreams: 1 1-s2.0-S0165022X03002057-main.pdf: 206713 bytes, checksum: d6f3ae6ffe05064843dd31f40a194e93 (MD5) Previous issue date: 2004Elservierhttp://dx.doi.org/10.1016/j.jbbm.2003.10.008reponame:Repositório Institucional da UFBAinstname:Universidade Federal da Bahia (UFBA)instacron:UFBALipid extractionProteinsSilicaDetergentComparison of six methods for the extraction of lipids from serum in terms of effectiveness and protein preservationJournal of Biochemical and Biophysical Methodsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article10000-01-01enginfo:eu-repo/semantics/openAccessORIGINAL1-s2.0-S0165022X03002057-main.pdf1-s2.0-S0165022X03002057-main.pdfapplication/pdf206713https://repositorio.ufba.br/bitstream/ri/7728/1/1-s2.0-S0165022X03002057-main.pdfd6f3ae6ffe05064843dd31f40a194e93MD51LICENSElicense.txtlicense.txttext/plain1762https://repositorio.ufba.br/bitstream/ri/7728/2/license.txt1b89a9a0548218172d7c829f87a0eab9MD52TEXT1-s2.0-S0165022X03002057-main.pdf.txt1-s2.0-S0165022X03002057-main.pdf.txtExtracted texttext/plain15099https://repositorio.ufba.br/bitstream/ri/7728/3/1-s2.0-S0165022X03002057-main.pdf.txte8cc5f34e2a0329ed4b1538aafe3ae5bMD53ri/77282022-07-05 14:03:22.305oai:repositorio.ufba.br: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Repositório InstitucionalPUBhttp://192.188.11.11:8080/oai/requestopendoar:19322022-07-05T17:03:22Repositório Institucional da UFBA - Universidade Federal da Bahia (UFBA)false |
dc.title.pt_BR.fl_str_mv |
Comparison of six methods for the extraction of lipids from serum in terms of effectiveness and protein preservation |
dc.title.alternative.pt_BR.fl_str_mv |
Journal of Biochemical and Biophysical Methods |
title |
Comparison of six methods for the extraction of lipids from serum in terms of effectiveness and protein preservation |
spellingShingle |
Comparison of six methods for the extraction of lipids from serum in terms of effectiveness and protein preservation Ferraz, T. P. L. Lipid extraction Proteins Silica Detergent |
title_short |
Comparison of six methods for the extraction of lipids from serum in terms of effectiveness and protein preservation |
title_full |
Comparison of six methods for the extraction of lipids from serum in terms of effectiveness and protein preservation |
title_fullStr |
Comparison of six methods for the extraction of lipids from serum in terms of effectiveness and protein preservation |
title_full_unstemmed |
Comparison of six methods for the extraction of lipids from serum in terms of effectiveness and protein preservation |
title_sort |
Comparison of six methods for the extraction of lipids from serum in terms of effectiveness and protein preservation |
author |
Ferraz, T. P. L. |
author_facet |
Ferraz, T. P. L. Fiúza, M. C. Santos, M. L. A. dos Carvalho, Lain Carlos Pontes de Soares, N. M. |
author_role |
author |
author2 |
Fiúza, M. C. Santos, M. L. A. dos Carvalho, Lain Carlos Pontes de Soares, N. M. |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Ferraz, T. P. L. Fiúza, M. C. Santos, M. L. A. dos Carvalho, Lain Carlos Pontes de Soares, N. M. Ferraz, T. P. L. Fiúza, M. C. Santos, M. L. A. dos Carvalho, Lain Carlos Pontes de Soares, N. M. |
dc.subject.por.fl_str_mv |
Lipid extraction Proteins Silica Detergent |
topic |
Lipid extraction Proteins Silica Detergent |
description |
Texto completo: acesso restrito. p. 187–193 |
publishDate |
2004 |
dc.date.issued.fl_str_mv |
2004 |
dc.date.accessioned.fl_str_mv |
2013-01-03T18:22:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.repositorio.ufba.br/ri/handle/ri/7728 |
dc.identifier.issn.none.fl_str_mv |
0165-022X |
dc.identifier.number.pt_BR.fl_str_mv |
v. 58, n. 3 |
identifier_str_mv |
0165-022X v. 58, n. 3 |
url |
http://www.repositorio.ufba.br/ri/handle/ri/7728 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
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openAccess |
dc.publisher.none.fl_str_mv |
Elservier |
publisher.none.fl_str_mv |
Elservier |
dc.source.pt_BR.fl_str_mv |
http://dx.doi.org/10.1016/j.jbbm.2003.10.008 |
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Repositório Institucional da UFBA |
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