GENETIC SIMILARITY OF Macrophomina pseudophaseolina ISOLATES ASSOCIATED WITH WEEDS IN THE BRAZILIAN SEMIARID REGION
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista Caatinga |
Texto Completo: | https://periodicos.ufersa.edu.br/caatinga/article/view/9353 |
Resumo: | Macrophomina pseudophaseolina has recently been reported in association with weeds in melon producing areas in Northeastern Brazil. Species from this genus are the causal agents of root rot and vine decline (RRVD) in melon, reducing its productivity. It is needed to know the genetic variability of the pathogen to develop effective control methods. Thus, this work aimed to assess the genetic diversity among M. pseudophaseolina isolates collected from the weeds Trianthema portulacastrum L. and Boerhavia diffusa L. using ISSR and RAPD markers. For this, 41 M. pseudophaseolina isolates were submitted to amplification with five ISSR and ten RAPD primers. Genetic similarity was analyzed using the Jaccard’s coefficient and cluster analysis was performed by the UPGMA method. Combining data from both markers, the 41 isolates were separated into eight groups. Most groups were not arranged according to geographical origin and host of the pathogen. The genetic similarity among isolates ranged from 0.15 to 0.87. On the other hand, the highest genetic dissimilarity (85%) was observed between the isolate MpBr11, collected from T. portulacastrum in Icapuí (CE), and MpBr65, collected from B. diffusa in Assú (RN). Results obtained herein can assist breeding programs for the selection of resistance sources and the development of effective control methods against RRVD in melon. |
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GENETIC SIMILARITY OF Macrophomina pseudophaseolina ISOLATES ASSOCIATED WITH WEEDS IN THE BRAZILIAN SEMIARID REGIONSIMILARIDADE GENÉTICA DE ISOLADOS DE Macrophomina pseudophaseolina ASSOCIADOS A PLANTAS DANINHAS NO SEMI-ÁRIDO BRASILEIROVariabilidade genética. RAPD. ISSR. Melão.Genetic variability. RAPD. ISSR. Melon.Macrophomina pseudophaseolina has recently been reported in association with weeds in melon producing areas in Northeastern Brazil. Species from this genus are the causal agents of root rot and vine decline (RRVD) in melon, reducing its productivity. It is needed to know the genetic variability of the pathogen to develop effective control methods. Thus, this work aimed to assess the genetic diversity among M. pseudophaseolina isolates collected from the weeds Trianthema portulacastrum L. and Boerhavia diffusa L. using ISSR and RAPD markers. For this, 41 M. pseudophaseolina isolates were submitted to amplification with five ISSR and ten RAPD primers. Genetic similarity was analyzed using the Jaccard’s coefficient and cluster analysis was performed by the UPGMA method. Combining data from both markers, the 41 isolates were separated into eight groups. Most groups were not arranged according to geographical origin and host of the pathogen. The genetic similarity among isolates ranged from 0.15 to 0.87. On the other hand, the highest genetic dissimilarity (85%) was observed between the isolate MpBr11, collected from T. portulacastrum in Icapuí (CE), and MpBr65, collected from B. diffusa in Assú (RN). Results obtained herein can assist breeding programs for the selection of resistance sources and the development of effective control methods against RRVD in melon. Macrophomina pseudophaseolina foi recentemente relatada em áreas produtoras de melão em associação com plantas daninhas na região Nordeste. Espécies do gênero são agentes da podridão radicular e declínio das ramas (PRDR) do meloeiro, doença que reduz sua produtividade. O conhecimento da diversidade genética do patógeno é importante no desenvolvimento de métodos de controle eficientes. Nesse contexto, o objetivo desse trabalho foi analisar a variabilidade genética de isolados de M. pseudophaseolina coletados das plantas daninhas Trianthema portulacastrum L. e Boerhavia diffusa L. por meio dos marcadores moleculares ISSR e RAPD. Para isto, 41 isolados de M. pseudophaseolina foram submetidos à amplificação com cinco iniciadores ISSR e dez RAPD. A similaridade genética foi analisada através do coeficiente de Jaccard e o agrupamento obtido com o método UPGMA. Com a combinação dos dados dos marcadores os 41 isolados foram agrupados em 8 grupos principais. Não foi observado, para grande maioria dos grupos gerados, a relação do agrupamento de acordo com hospedeiro ou local de coleta. A similaridade genética entre os isolados variou de 0,15 a 0,87. A maior dissimilaridade genética (85%) foi observada entre o isolado MpBr11, coletado de T. portulacastrum no município de Icapuí/CE, do isolado MpBr65, coletado de B. diffusa em Assú/RN. Estas informações podem ser úteis para auxiliar programas de melhoramento genético na seleção de fontes de resistência e/ou em testes de métodos de controle contra PRDR do melão. Universidade Federal Rural do Semi-Árido2020-10-21info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://periodicos.ufersa.edu.br/caatinga/article/view/935310.1590/1983-21252020v33n405rcREVISTA CAATINGA; Vol. 33 No. 4 (2020); 908-917Revista Caatinga; v. 33 n. 4 (2020); 908-9171983-21250100-316Xreponame:Revista Caatingainstname:Universidade Federal Rural do Semi-Árido (UFERSA)instacron:UFERSAenghttps://periodicos.ufersa.edu.br/caatinga/article/view/9353/10357Copyright (c) 2020 Revista Caatingainfo:eu-repo/semantics/openAccessCosta, Talison Eugênio daNegreiros, Andréia Mitsa PaivaSouza, Matheus de FreitasSales Júnior, Rui Holanda, Ioná Santos Araújo2023-07-21T16:40:05Zoai:ojs.periodicos.ufersa.edu.br:article/9353Revistahttps://periodicos.ufersa.edu.br/index.php/caatinga/indexPUBhttps://periodicos.ufersa.edu.br/index.php/caatinga/oaipatricio@ufersa.edu.br|| caatinga@ufersa.edu.br1983-21250100-316Xopendoar:2024-04-29T09:46:48.263215Revista Caatinga - Universidade Federal Rural do Semi-Árido (UFERSA)true |
dc.title.none.fl_str_mv |
GENETIC SIMILARITY OF Macrophomina pseudophaseolina ISOLATES ASSOCIATED WITH WEEDS IN THE BRAZILIAN SEMIARID REGION SIMILARIDADE GENÉTICA DE ISOLADOS DE Macrophomina pseudophaseolina ASSOCIADOS A PLANTAS DANINHAS NO SEMI-ÁRIDO BRASILEIRO |
title |
GENETIC SIMILARITY OF Macrophomina pseudophaseolina ISOLATES ASSOCIATED WITH WEEDS IN THE BRAZILIAN SEMIARID REGION |
spellingShingle |
GENETIC SIMILARITY OF Macrophomina pseudophaseolina ISOLATES ASSOCIATED WITH WEEDS IN THE BRAZILIAN SEMIARID REGION Costa, Talison Eugênio da Variabilidade genética. RAPD. ISSR. Melão. Genetic variability. RAPD. ISSR. Melon. |
title_short |
GENETIC SIMILARITY OF Macrophomina pseudophaseolina ISOLATES ASSOCIATED WITH WEEDS IN THE BRAZILIAN SEMIARID REGION |
title_full |
GENETIC SIMILARITY OF Macrophomina pseudophaseolina ISOLATES ASSOCIATED WITH WEEDS IN THE BRAZILIAN SEMIARID REGION |
title_fullStr |
GENETIC SIMILARITY OF Macrophomina pseudophaseolina ISOLATES ASSOCIATED WITH WEEDS IN THE BRAZILIAN SEMIARID REGION |
title_full_unstemmed |
GENETIC SIMILARITY OF Macrophomina pseudophaseolina ISOLATES ASSOCIATED WITH WEEDS IN THE BRAZILIAN SEMIARID REGION |
title_sort |
GENETIC SIMILARITY OF Macrophomina pseudophaseolina ISOLATES ASSOCIATED WITH WEEDS IN THE BRAZILIAN SEMIARID REGION |
author |
Costa, Talison Eugênio da |
author_facet |
Costa, Talison Eugênio da Negreiros, Andréia Mitsa Paiva Souza, Matheus de Freitas Sales Júnior, Rui Holanda, Ioná Santos Araújo |
author_role |
author |
author2 |
Negreiros, Andréia Mitsa Paiva Souza, Matheus de Freitas Sales Júnior, Rui Holanda, Ioná Santos Araújo |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Costa, Talison Eugênio da Negreiros, Andréia Mitsa Paiva Souza, Matheus de Freitas Sales Júnior, Rui Holanda, Ioná Santos Araújo |
dc.subject.por.fl_str_mv |
Variabilidade genética. RAPD. ISSR. Melão. Genetic variability. RAPD. ISSR. Melon. |
topic |
Variabilidade genética. RAPD. ISSR. Melão. Genetic variability. RAPD. ISSR. Melon. |
description |
Macrophomina pseudophaseolina has recently been reported in association with weeds in melon producing areas in Northeastern Brazil. Species from this genus are the causal agents of root rot and vine decline (RRVD) in melon, reducing its productivity. It is needed to know the genetic variability of the pathogen to develop effective control methods. Thus, this work aimed to assess the genetic diversity among M. pseudophaseolina isolates collected from the weeds Trianthema portulacastrum L. and Boerhavia diffusa L. using ISSR and RAPD markers. For this, 41 M. pseudophaseolina isolates were submitted to amplification with five ISSR and ten RAPD primers. Genetic similarity was analyzed using the Jaccard’s coefficient and cluster analysis was performed by the UPGMA method. Combining data from both markers, the 41 isolates were separated into eight groups. Most groups were not arranged according to geographical origin and host of the pathogen. The genetic similarity among isolates ranged from 0.15 to 0.87. On the other hand, the highest genetic dissimilarity (85%) was observed between the isolate MpBr11, collected from T. portulacastrum in Icapuí (CE), and MpBr65, collected from B. diffusa in Assú (RN). Results obtained herein can assist breeding programs for the selection of resistance sources and the development of effective control methods against RRVD in melon. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-10-21 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://periodicos.ufersa.edu.br/caatinga/article/view/9353 10.1590/1983-21252020v33n405rc |
url |
https://periodicos.ufersa.edu.br/caatinga/article/view/9353 |
identifier_str_mv |
10.1590/1983-21252020v33n405rc |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://periodicos.ufersa.edu.br/caatinga/article/view/9353/10357 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2020 Revista Caatinga info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2020 Revista Caatinga |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal Rural do Semi-Árido |
publisher.none.fl_str_mv |
Universidade Federal Rural do Semi-Árido |
dc.source.none.fl_str_mv |
REVISTA CAATINGA; Vol. 33 No. 4 (2020); 908-917 Revista Caatinga; v. 33 n. 4 (2020); 908-917 1983-2125 0100-316X reponame:Revista Caatinga instname:Universidade Federal Rural do Semi-Árido (UFERSA) instacron:UFERSA |
instname_str |
Universidade Federal Rural do Semi-Árido (UFERSA) |
instacron_str |
UFERSA |
institution |
UFERSA |
reponame_str |
Revista Caatinga |
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Revista Caatinga |
repository.name.fl_str_mv |
Revista Caatinga - Universidade Federal Rural do Semi-Árido (UFERSA) |
repository.mail.fl_str_mv |
patricio@ufersa.edu.br|| caatinga@ufersa.edu.br |
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1797674028488458240 |