PREGNANCY RATES USING FROZEN SEMEN OF CRIOLLO STALLIONS WITH GLYCEROL OR DIMETHYLFORMAMID AS CRYOPROTECTANTS
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Ciência animal brasileira (Online) |
Texto Completo: | https://revistas.ufg.br/vet/article/view/18923 |
Resumo: | Pregnancy rates using stallions’s frozen semen are variable; moreover, some breeds present low freezability. One hundred and four (104) mares were inseminated and separeted into two experiments to evaluate fertility of frozen semen’s of Criollo stallions (n=5), with 5% of dimethylformamide (DMF) or 5% of glycerol (GLY) as cryoprotectants. In Experiment I, the inseminations were made during pre-ovulation with fresh and frozen semen with DMF. In Experiment II, the mares were inseminated during post-ovulation with fresh semen, DMF and GL. The inseminations with frozen semen were performed deep in the uterine horn ipsilateralis to the dominant follicle. Control mares were inseminated with fresh semen in the uterus. Only one estrus period per mare was used to evaluate the pregnancy rates of the groups. Pregnancy diagnosis through ultrasonography was performed on the 15th day post-ovulation. The mean post-thawing motility was 40% and 20%, respectively, for frozen semen with DMF and GLY (P<0.05). All stallions had higher post-thawing motility when using DMF. In Experiment I, pregnancy rates were 12% (5/42) and 62% (20/32), respectively for DMF and fresh semen (P<0.0001). In Experiment II, pregnancy rates were 40% (4/10), 10% (1/10) and 70% (7/10), respectively for DMF, GLY (P<0.05) and fresh semen (P<0.0001). Insemination with frozen semen performed with more frequent follicular control showed the best results. The low sperm motility after thawing was attributed to the GLY used in experiment II. The DMF can be used alternatively to Criollos Stallion’s semen feezing.KEYWORDS: Artificial insemination; cryopreservation; cryoprotectant; equine; spermatozoa. |
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PREGNANCY RATES USING FROZEN SEMEN OF CRIOLLO STALLIONS WITH GLYCEROL OR DIMETHYLFORMAMID AS CRYOPROTECTANTSÍNDICE DE PRENHEZ COM SÊMEN CONGELADO DE GARANHÕES DA RAÇA CRIOULA USANDO GLICEROL OU DIMETILFORMAMIDA COMO CRIOPROTETORESAnimal reproductionamidascriopreservaçãoequinoespermatozóidesinseminação artificialPregnancy rates using stallions’s frozen semen are variable; moreover, some breeds present low freezability. One hundred and four (104) mares were inseminated and separeted into two experiments to evaluate fertility of frozen semen’s of Criollo stallions (n=5), with 5% of dimethylformamide (DMF) or 5% of glycerol (GLY) as cryoprotectants. In Experiment I, the inseminations were made during pre-ovulation with fresh and frozen semen with DMF. In Experiment II, the mares were inseminated during post-ovulation with fresh semen, DMF and GL. The inseminations with frozen semen were performed deep in the uterine horn ipsilateralis to the dominant follicle. Control mares were inseminated with fresh semen in the uterus. Only one estrus period per mare was used to evaluate the pregnancy rates of the groups. Pregnancy diagnosis through ultrasonography was performed on the 15th day post-ovulation. The mean post-thawing motility was 40% and 20%, respectively, for frozen semen with DMF and GLY (P<0.05). All stallions had higher post-thawing motility when using DMF. In Experiment I, pregnancy rates were 12% (5/42) and 62% (20/32), respectively for DMF and fresh semen (P<0.0001). In Experiment II, pregnancy rates were 40% (4/10), 10% (1/10) and 70% (7/10), respectively for DMF, GLY (P<0.05) and fresh semen (P<0.0001). Insemination with frozen semen performed with more frequent follicular control showed the best results. The low sperm motility after thawing was attributed to the GLY used in experiment II. The DMF can be used alternatively to Criollos Stallion’s semen feezing.KEYWORDS: Artificial insemination; cryopreservation; cryoprotectant; equine; spermatozoa. O índice de prenhez utilizando sêmen criopreservado de garanhões é variável e, além disso, algumas raças apresentam baixa congelabilidade. Foram inseminadas 104 éguas, divididas em dois experimentos, para avaliar a fertilidade do sêmen congelado de garanhões da raça Crioula (n=5), com 5% de dimetilformamida (DMF) ou 5% de glicerol (GLI), como crioprotetores. No Experimento I, as inseminações foram conduzidas pré-ovulação com sêmen fresco e criopreservado com DMF. No Experimento II, as éguas foram inseminadas pós-ovulação com sêmen fresco, DMF e GLI. As inseminações com sêmen congelado foram realizadas no ápice do corno uterino e as éguas do grupo controle foram inseminadas no corpo do útero com sêmen fresco. Para a avaliação dos índices de prenhez dos grupos, utilizou-se um ciclo estral/animal. O diagnóstico de gestação foi realizado por meio de ultrassonografia transretal no 15o dia pós-ovulação. A motilidade média pós-descongelamento foi de 40% e 20%, respectivamente, para o sêmen congelado com DMF e GLI (P<0,05). Todos os garanhões tiveram motilidade superior no pós-descongelamento quando se utilizou a DMF. No Experimento I, o índice de prenhez foi de 12% (5/42) e 62% (20/32), respectivamente, para DMF e sêmen fresco (P<0,0001). No Experimento II, o índice de prenhez foi de 70% (7/10; P<0,05) para o sêmen fresco, 40% para o congelado com DMF (4/10; P<0,05) e 10% com GLI (1/10). A inseminação com sêmen congelado realizada com controle folicular mais frequente apresentou os melhores resultados. A baixa motilidade dos espermatozoides pós-descongelamento foi atribuída ao GLI utilizado no Experimento II. A DMF pode ser utilizada como uma alternativa ao congelamento do sêmen de garanhões da raça Crioula.PALAVRAS-CHAVE: Criopreservação; crioprotetores; equino; espermatozoide; inseminação artificial. Universidade Federal de Goiás2013-12-17info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionresearchAvaliado por paresPesquisa Científicaapplication/pdftext/htmlhttps://revistas.ufg.br/vet/article/view/1892310.5216/cab.v14i4.18923Brazilian Animal Science/ Ciência Animal Brasileira; Vol. 14 No. 4 (2013); 488-494Ciência Animal Brasileira / Brazilian Animal Science; v. 14 n. 4 (2013); 488-4941809-68911518-2797reponame:Ciência animal brasileira (Online)instname:Universidade Federal de Goiás (UFG)instacron:UFGporhttps://revistas.ufg.br/vet/article/view/18923/15642https://revistas.ufg.br/vet/article/view/18923/15643Oliveira, Rodrigo ArrudaRubin, Mara Iolanda BatistellaSilva, Carlos Antonio Mondinoinfo:eu-repo/semantics/openAccess2013-12-17T13:26:53Zoai:ojs.revistas.ufg.br:article/18923Revistahttps://revistas.ufg.br/vetPUBhttps://revistas.ufg.br/vet/oai||revistacab@gmail.com1809-68911518-2797opendoar:2013-12-17T13:26:53Ciência animal brasileira (Online) - Universidade Federal de Goiás (UFG)false |
dc.title.none.fl_str_mv |
PREGNANCY RATES USING FROZEN SEMEN OF CRIOLLO STALLIONS WITH GLYCEROL OR DIMETHYLFORMAMID AS CRYOPROTECTANTS ÍNDICE DE PRENHEZ COM SÊMEN CONGELADO DE GARANHÕES DA RAÇA CRIOULA USANDO GLICEROL OU DIMETILFORMAMIDA COMO CRIOPROTETORES |
title |
PREGNANCY RATES USING FROZEN SEMEN OF CRIOLLO STALLIONS WITH GLYCEROL OR DIMETHYLFORMAMID AS CRYOPROTECTANTS |
spellingShingle |
PREGNANCY RATES USING FROZEN SEMEN OF CRIOLLO STALLIONS WITH GLYCEROL OR DIMETHYLFORMAMID AS CRYOPROTECTANTS Oliveira, Rodrigo Arruda Animal reproduction amidas criopreservação equino espermatozóides inseminação artificial |
title_short |
PREGNANCY RATES USING FROZEN SEMEN OF CRIOLLO STALLIONS WITH GLYCEROL OR DIMETHYLFORMAMID AS CRYOPROTECTANTS |
title_full |
PREGNANCY RATES USING FROZEN SEMEN OF CRIOLLO STALLIONS WITH GLYCEROL OR DIMETHYLFORMAMID AS CRYOPROTECTANTS |
title_fullStr |
PREGNANCY RATES USING FROZEN SEMEN OF CRIOLLO STALLIONS WITH GLYCEROL OR DIMETHYLFORMAMID AS CRYOPROTECTANTS |
title_full_unstemmed |
PREGNANCY RATES USING FROZEN SEMEN OF CRIOLLO STALLIONS WITH GLYCEROL OR DIMETHYLFORMAMID AS CRYOPROTECTANTS |
title_sort |
PREGNANCY RATES USING FROZEN SEMEN OF CRIOLLO STALLIONS WITH GLYCEROL OR DIMETHYLFORMAMID AS CRYOPROTECTANTS |
author |
Oliveira, Rodrigo Arruda |
author_facet |
Oliveira, Rodrigo Arruda Rubin, Mara Iolanda Batistella Silva, Carlos Antonio Mondino |
author_role |
author |
author2 |
Rubin, Mara Iolanda Batistella Silva, Carlos Antonio Mondino |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Oliveira, Rodrigo Arruda Rubin, Mara Iolanda Batistella Silva, Carlos Antonio Mondino |
dc.subject.por.fl_str_mv |
Animal reproduction amidas criopreservação equino espermatozóides inseminação artificial |
topic |
Animal reproduction amidas criopreservação equino espermatozóides inseminação artificial |
description |
Pregnancy rates using stallions’s frozen semen are variable; moreover, some breeds present low freezability. One hundred and four (104) mares were inseminated and separeted into two experiments to evaluate fertility of frozen semen’s of Criollo stallions (n=5), with 5% of dimethylformamide (DMF) or 5% of glycerol (GLY) as cryoprotectants. In Experiment I, the inseminations were made during pre-ovulation with fresh and frozen semen with DMF. In Experiment II, the mares were inseminated during post-ovulation with fresh semen, DMF and GL. The inseminations with frozen semen were performed deep in the uterine horn ipsilateralis to the dominant follicle. Control mares were inseminated with fresh semen in the uterus. Only one estrus period per mare was used to evaluate the pregnancy rates of the groups. Pregnancy diagnosis through ultrasonography was performed on the 15th day post-ovulation. The mean post-thawing motility was 40% and 20%, respectively, for frozen semen with DMF and GLY (P<0.05). All stallions had higher post-thawing motility when using DMF. In Experiment I, pregnancy rates were 12% (5/42) and 62% (20/32), respectively for DMF and fresh semen (P<0.0001). In Experiment II, pregnancy rates were 40% (4/10), 10% (1/10) and 70% (7/10), respectively for DMF, GLY (P<0.05) and fresh semen (P<0.0001). Insemination with frozen semen performed with more frequent follicular control showed the best results. The low sperm motility after thawing was attributed to the GLY used in experiment II. The DMF can be used alternatively to Criollos Stallion’s semen feezing.KEYWORDS: Artificial insemination; cryopreservation; cryoprotectant; equine; spermatozoa. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-12-17 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion research Avaliado por pares Pesquisa Científica |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://revistas.ufg.br/vet/article/view/18923 10.5216/cab.v14i4.18923 |
url |
https://revistas.ufg.br/vet/article/view/18923 |
identifier_str_mv |
10.5216/cab.v14i4.18923 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://revistas.ufg.br/vet/article/view/18923/15642 https://revistas.ufg.br/vet/article/view/18923/15643 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf text/html |
dc.publisher.none.fl_str_mv |
Universidade Federal de Goiás |
publisher.none.fl_str_mv |
Universidade Federal de Goiás |
dc.source.none.fl_str_mv |
Brazilian Animal Science/ Ciência Animal Brasileira; Vol. 14 No. 4 (2013); 488-494 Ciência Animal Brasileira / Brazilian Animal Science; v. 14 n. 4 (2013); 488-494 1809-6891 1518-2797 reponame:Ciência animal brasileira (Online) instname:Universidade Federal de Goiás (UFG) instacron:UFG |
instname_str |
Universidade Federal de Goiás (UFG) |
instacron_str |
UFG |
institution |
UFG |
reponame_str |
Ciência animal brasileira (Online) |
collection |
Ciência animal brasileira (Online) |
repository.name.fl_str_mv |
Ciência animal brasileira (Online) - Universidade Federal de Goiás (UFG) |
repository.mail.fl_str_mv |
||revistacab@gmail.com |
_version_ |
1789440221009412096 |