Detection of mutations within exons 4 to 8 of the p53 tumor suppressor gene in canine mammary glands
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Arquivo brasileiro de medicina veterinária e zootecnia (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352012000200013 |
Resumo: | Fifteen female canines with mammary tumors and 6 normal females were used to study mutations in exons 4 to 8 of the p53 gene. DNA samples from the tumors, respective adjacent normal mammary tissue and mammary glands from healthy animals were sequenced and analyzed for the presence of mutations. Mutations were found in 71.8% of the samples and the most frequent were missense mutations. The most attacked exons in the mammary tumor were 5, 7 and 8, with 23.4, 31.6 and 23.4% mutations, respectively. Canine mammary tumors are related to mutations in gene p53 and mutations mostly occur in the region of the protein that is linked to the DNA in the cell nucleus, which can change the functionality of the cell and propitiate tumor growth. Despite being macroscopically normal, the mammary tissue adjacent to the tumors has mutations that can lead to recurrence if not removed together with the tumor. |
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Arquivo brasileiro de medicina veterinária e zootecnia (Online) |
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Detection of mutations within exons 4 to 8 of the p53 tumor suppressor gene in canine mammary glandsbitchDNAmammamolecular biologyneoplasmPCRFifteen female canines with mammary tumors and 6 normal females were used to study mutations in exons 4 to 8 of the p53 gene. DNA samples from the tumors, respective adjacent normal mammary tissue and mammary glands from healthy animals were sequenced and analyzed for the presence of mutations. Mutations were found in 71.8% of the samples and the most frequent were missense mutations. The most attacked exons in the mammary tumor were 5, 7 and 8, with 23.4, 31.6 and 23.4% mutations, respectively. Canine mammary tumors are related to mutations in gene p53 and mutations mostly occur in the region of the protein that is linked to the DNA in the cell nucleus, which can change the functionality of the cell and propitiate tumor growth. Despite being macroscopically normal, the mammary tissue adjacent to the tumors has mutations that can lead to recurrence if not removed together with the tumor.Universidade Federal de Minas Gerais, Escola de Veterinária2012-04-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352012000200013Arquivo Brasileiro de Medicina Veterinária e Zootecnia v.64 n.2 2012reponame:Arquivo brasileiro de medicina veterinária e zootecnia (Online)instname:Universidade Federal de Minas Gerais (UFMG)instacron:UFMG10.1590/S0102-09352012000200013info:eu-repo/semantics/openAccessSouza,D.M.B.Barros,M.G.O.Silva,J.S.C.Silva,M.B.Coleto,Z.F.Jimenez,G.C.Adrião,M.Wischral,A.eng2012-05-07T00:00:00Zoai:scielo:S0102-09352012000200013Revistahttps://www.scielo.br/j/abmvz/PUBhttps://old.scielo.br/oai/scielo-oai.phpjournal@vet.ufmg.br||abmvz.artigo@abmvz.org.br1678-41620102-0935opendoar:2012-05-07T00:00Arquivo brasileiro de medicina veterinária e zootecnia (Online) - Universidade Federal de Minas Gerais (UFMG)false |
dc.title.none.fl_str_mv |
Detection of mutations within exons 4 to 8 of the p53 tumor suppressor gene in canine mammary glands |
title |
Detection of mutations within exons 4 to 8 of the p53 tumor suppressor gene in canine mammary glands |
spellingShingle |
Detection of mutations within exons 4 to 8 of the p53 tumor suppressor gene in canine mammary glands Souza,D.M.B. bitch DNA mamma molecular biology neoplasm PCR |
title_short |
Detection of mutations within exons 4 to 8 of the p53 tumor suppressor gene in canine mammary glands |
title_full |
Detection of mutations within exons 4 to 8 of the p53 tumor suppressor gene in canine mammary glands |
title_fullStr |
Detection of mutations within exons 4 to 8 of the p53 tumor suppressor gene in canine mammary glands |
title_full_unstemmed |
Detection of mutations within exons 4 to 8 of the p53 tumor suppressor gene in canine mammary glands |
title_sort |
Detection of mutations within exons 4 to 8 of the p53 tumor suppressor gene in canine mammary glands |
author |
Souza,D.M.B. |
author_facet |
Souza,D.M.B. Barros,M.G.O. Silva,J.S.C. Silva,M.B. Coleto,Z.F. Jimenez,G.C. Adrião,M. Wischral,A. |
author_role |
author |
author2 |
Barros,M.G.O. Silva,J.S.C. Silva,M.B. Coleto,Z.F. Jimenez,G.C. Adrião,M. Wischral,A. |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Souza,D.M.B. Barros,M.G.O. Silva,J.S.C. Silva,M.B. Coleto,Z.F. Jimenez,G.C. Adrião,M. Wischral,A. |
dc.subject.por.fl_str_mv |
bitch DNA mamma molecular biology neoplasm PCR |
topic |
bitch DNA mamma molecular biology neoplasm PCR |
description |
Fifteen female canines with mammary tumors and 6 normal females were used to study mutations in exons 4 to 8 of the p53 gene. DNA samples from the tumors, respective adjacent normal mammary tissue and mammary glands from healthy animals were sequenced and analyzed for the presence of mutations. Mutations were found in 71.8% of the samples and the most frequent were missense mutations. The most attacked exons in the mammary tumor were 5, 7 and 8, with 23.4, 31.6 and 23.4% mutations, respectively. Canine mammary tumors are related to mutations in gene p53 and mutations mostly occur in the region of the protein that is linked to the DNA in the cell nucleus, which can change the functionality of the cell and propitiate tumor growth. Despite being macroscopically normal, the mammary tissue adjacent to the tumors has mutations that can lead to recurrence if not removed together with the tumor. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-04-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352012000200013 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352012000200013 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0102-09352012000200013 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Universidade Federal de Minas Gerais, Escola de Veterinária |
publisher.none.fl_str_mv |
Universidade Federal de Minas Gerais, Escola de Veterinária |
dc.source.none.fl_str_mv |
Arquivo Brasileiro de Medicina Veterinária e Zootecnia v.64 n.2 2012 reponame:Arquivo brasileiro de medicina veterinária e zootecnia (Online) instname:Universidade Federal de Minas Gerais (UFMG) instacron:UFMG |
instname_str |
Universidade Federal de Minas Gerais (UFMG) |
instacron_str |
UFMG |
institution |
UFMG |
reponame_str |
Arquivo brasileiro de medicina veterinária e zootecnia (Online) |
collection |
Arquivo brasileiro de medicina veterinária e zootecnia (Online) |
repository.name.fl_str_mv |
Arquivo brasileiro de medicina veterinária e zootecnia (Online) - Universidade Federal de Minas Gerais (UFMG) |
repository.mail.fl_str_mv |
journal@vet.ufmg.br||abmvz.artigo@abmvz.org.br |
_version_ |
1750220885079359488 |