Aqueous extract of Baccharis trimera improves redox status and decreases the severity of alcoholic hepatotoxicity.
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFOP |
Texto Completo: | http://www.repositorio.ufop.br/handle/123456789/10907 |
Resumo: | The metabolism of ethanol occurs mainly in the liver, promoting increase of reactive oxygen species and nitrogen, leading to redox imbalance. Therefore, antioxidants can be seen as an alternative to reestablish the oxidizing/reducing equilibrium. The aim of this study was to evaluate the antioxidant and hepatoprotective effect of aqueous extract of Baccharis trimera (Less.) DC., Asteraceae, in a model of hepatotoxicity induced by ethanol. The extract was characterized and in vitro tests were conducted in HepG2 cells. It was evaluated the cells viability exposed to aqueous extract for 24 h, ability to scavenging the radical DPPH, besides the production of reactive oxygen species and nitric oxide, and the influence on the transcriptional activity of transcription factor Nrf2 (12 and 24 h) after exposure to 200 mM ethanol. The results showed that aqueous extract was non-cytotoxic in any concentration tested; moreover, it was observed a decrease in ROS and NO production, also promoting the transcriptional activity of Nrf2. In vivo, we pretreatment male rats Fisher with 600 mg/kg of aqueous extract and 1 h later 5 ml/kg of absolute ethanol was administrated. After two days of treatment, the animals were euthanized and lipid profile, hepatic and renal functions, antioxidant status and oxidative damage were evaluated. The treatment with extract improved liver function and lipid profile, reflecting the reduction of lipid microvesicules in the liver. It also promoted an increase of glutathione peroxidase activity, decrease of oxidative damage and MMP2 activity. These results, analyzed together, suggest the hepatoprotective effect of B. trimera aqueous extract. |
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Rabelo, Ana Carolina SilveiraAraújo, Glaucy Rodrigues deLúcio, Karine de PáduaAraújo, Carolina MoraisMiranda, Pedro Henrique de AmorimSilva, Breno de MelloCarneiro, Ana Cláudia AlvarengaRibeiro, Erica Milena de CastroLima, Wanderson Geraldo deSouza, Gustavo Henrique Bianco deBrandão, Geraldo CélioCosta, Daniela Caldeira2019-04-02T17:58:51Z2019-04-02T17:58:51Z2017RABELO, A. C. S. et al. Aqueous extract of Baccharis trimera improves redox status and decreases the severity of alcoholic hepatotoxicity. Revista Brasileira de Farmacognosia, v. 27, n. 6, p. 729-738, nov./dez. 2017. Disponível em: <https://www.sciencedirect.com/science/article/pii/S0102695X17303678>. Acesso em: 22 fev. 2019.0102695Xhttp://www.repositorio.ufop.br/handle/123456789/10907The metabolism of ethanol occurs mainly in the liver, promoting increase of reactive oxygen species and nitrogen, leading to redox imbalance. Therefore, antioxidants can be seen as an alternative to reestablish the oxidizing/reducing equilibrium. The aim of this study was to evaluate the antioxidant and hepatoprotective effect of aqueous extract of Baccharis trimera (Less.) DC., Asteraceae, in a model of hepatotoxicity induced by ethanol. The extract was characterized and in vitro tests were conducted in HepG2 cells. It was evaluated the cells viability exposed to aqueous extract for 24 h, ability to scavenging the radical DPPH, besides the production of reactive oxygen species and nitric oxide, and the influence on the transcriptional activity of transcription factor Nrf2 (12 and 24 h) after exposure to 200 mM ethanol. The results showed that aqueous extract was non-cytotoxic in any concentration tested; moreover, it was observed a decrease in ROS and NO production, also promoting the transcriptional activity of Nrf2. In vivo, we pretreatment male rats Fisher with 600 mg/kg of aqueous extract and 1 h later 5 ml/kg of absolute ethanol was administrated. After two days of treatment, the animals were euthanized and lipid profile, hepatic and renal functions, antioxidant status and oxidative damage were evaluated. The treatment with extract improved liver function and lipid profile, reflecting the reduction of lipid microvesicules in the liver. It also promoted an increase of glutathione peroxidase activity, decrease of oxidative damage and MMP2 activity. These results, analyzed together, suggest the hepatoprotective effect of B. trimera aqueous extract.This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). Fonte: o próprio artigoinfo:eu-repo/semantics/openAccessEthanolRedox imbalanceAqueous extract of Baccharis trimera improves redox status and decreases the severity of alcoholic hepatotoxicity.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleengreponame:Repositório Institucional da UFOPinstname:Universidade Federal de Ouro Preto (UFOP)instacron:UFOPLICENSElicense.txtlicense.txttext/plain; charset=utf-8924http://www.repositorio.ufop.br/bitstream/123456789/10907/2/license.txt62604f8d955274beb56c80ce1ee5dcaeMD52ORIGINALARTIGO_AqueousExtractBaccharis.pdfARTIGO_AqueousExtractBaccharis.pdfapplication/pdf1333414http://www.repositorio.ufop.br/bitstream/123456789/10907/1/ARTIGO_AqueousExtractBaccharis.pdf821ba19ab1f2c94b9a2a6e9c5a15f2b7MD51123456789/109072019-04-02 13:58:51.066oai:localhost: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ório InstitucionalPUBhttp://www.repositorio.ufop.br/oai/requestrepositorio@ufop.edu.bropendoar:32332019-04-02T17:58:51Repositório Institucional da UFOP - Universidade Federal de Ouro Preto (UFOP)false |
dc.title.pt_BR.fl_str_mv |
Aqueous extract of Baccharis trimera improves redox status and decreases the severity of alcoholic hepatotoxicity. |
title |
Aqueous extract of Baccharis trimera improves redox status and decreases the severity of alcoholic hepatotoxicity. |
spellingShingle |
Aqueous extract of Baccharis trimera improves redox status and decreases the severity of alcoholic hepatotoxicity. Rabelo, Ana Carolina Silveira Ethanol Redox imbalance |
title_short |
Aqueous extract of Baccharis trimera improves redox status and decreases the severity of alcoholic hepatotoxicity. |
title_full |
Aqueous extract of Baccharis trimera improves redox status and decreases the severity of alcoholic hepatotoxicity. |
title_fullStr |
Aqueous extract of Baccharis trimera improves redox status and decreases the severity of alcoholic hepatotoxicity. |
title_full_unstemmed |
Aqueous extract of Baccharis trimera improves redox status and decreases the severity of alcoholic hepatotoxicity. |
title_sort |
Aqueous extract of Baccharis trimera improves redox status and decreases the severity of alcoholic hepatotoxicity. |
author |
Rabelo, Ana Carolina Silveira |
author_facet |
Rabelo, Ana Carolina Silveira Araújo, Glaucy Rodrigues de Lúcio, Karine de Pádua Araújo, Carolina Morais Miranda, Pedro Henrique de Amorim Silva, Breno de Mello Carneiro, Ana Cláudia Alvarenga Ribeiro, Erica Milena de Castro Lima, Wanderson Geraldo de Souza, Gustavo Henrique Bianco de Brandão, Geraldo Célio Costa, Daniela Caldeira |
author_role |
author |
author2 |
Araújo, Glaucy Rodrigues de Lúcio, Karine de Pádua Araújo, Carolina Morais Miranda, Pedro Henrique de Amorim Silva, Breno de Mello Carneiro, Ana Cláudia Alvarenga Ribeiro, Erica Milena de Castro Lima, Wanderson Geraldo de Souza, Gustavo Henrique Bianco de Brandão, Geraldo Célio Costa, Daniela Caldeira |
author2_role |
author author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Rabelo, Ana Carolina Silveira Araújo, Glaucy Rodrigues de Lúcio, Karine de Pádua Araújo, Carolina Morais Miranda, Pedro Henrique de Amorim Silva, Breno de Mello Carneiro, Ana Cláudia Alvarenga Ribeiro, Erica Milena de Castro Lima, Wanderson Geraldo de Souza, Gustavo Henrique Bianco de Brandão, Geraldo Célio Costa, Daniela Caldeira |
dc.subject.por.fl_str_mv |
Ethanol Redox imbalance |
topic |
Ethanol Redox imbalance |
description |
The metabolism of ethanol occurs mainly in the liver, promoting increase of reactive oxygen species and nitrogen, leading to redox imbalance. Therefore, antioxidants can be seen as an alternative to reestablish the oxidizing/reducing equilibrium. The aim of this study was to evaluate the antioxidant and hepatoprotective effect of aqueous extract of Baccharis trimera (Less.) DC., Asteraceae, in a model of hepatotoxicity induced by ethanol. The extract was characterized and in vitro tests were conducted in HepG2 cells. It was evaluated the cells viability exposed to aqueous extract for 24 h, ability to scavenging the radical DPPH, besides the production of reactive oxygen species and nitric oxide, and the influence on the transcriptional activity of transcription factor Nrf2 (12 and 24 h) after exposure to 200 mM ethanol. The results showed that aqueous extract was non-cytotoxic in any concentration tested; moreover, it was observed a decrease in ROS and NO production, also promoting the transcriptional activity of Nrf2. In vivo, we pretreatment male rats Fisher with 600 mg/kg of aqueous extract and 1 h later 5 ml/kg of absolute ethanol was administrated. After two days of treatment, the animals were euthanized and lipid profile, hepatic and renal functions, antioxidant status and oxidative damage were evaluated. The treatment with extract improved liver function and lipid profile, reflecting the reduction of lipid microvesicules in the liver. It also promoted an increase of glutathione peroxidase activity, decrease of oxidative damage and MMP2 activity. These results, analyzed together, suggest the hepatoprotective effect of B. trimera aqueous extract. |
publishDate |
2017 |
dc.date.issued.fl_str_mv |
2017 |
dc.date.accessioned.fl_str_mv |
2019-04-02T17:58:51Z |
dc.date.available.fl_str_mv |
2019-04-02T17:58:51Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
RABELO, A. C. S. et al. Aqueous extract of Baccharis trimera improves redox status and decreases the severity of alcoholic hepatotoxicity. Revista Brasileira de Farmacognosia, v. 27, n. 6, p. 729-738, nov./dez. 2017. Disponível em: <https://www.sciencedirect.com/science/article/pii/S0102695X17303678>. Acesso em: 22 fev. 2019. |
dc.identifier.uri.fl_str_mv |
http://www.repositorio.ufop.br/handle/123456789/10907 |
dc.identifier.issn.none.fl_str_mv |
0102695X |
identifier_str_mv |
RABELO, A. C. S. et al. Aqueous extract of Baccharis trimera improves redox status and decreases the severity of alcoholic hepatotoxicity. Revista Brasileira de Farmacognosia, v. 27, n. 6, p. 729-738, nov./dez. 2017. Disponível em: <https://www.sciencedirect.com/science/article/pii/S0102695X17303678>. Acesso em: 22 fev. 2019. 0102695X |
url |
http://www.repositorio.ufop.br/handle/123456789/10907 |
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eng |
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eng |
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openAccess |
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