Gene expression analysis identifies hypothetical genes that may be critical during the infection process of Xanthomonas citri subsp. citri.
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFOP |
Texto Completo: | http://www.repositorio.ufop.br/handle/123456789/12030 https://doi.org/10.1016/j.ejbt.2019.10.003 |
Resumo: | Background: Gene expression analysis via microarray is widely used in phytobacteria to validate differential gene expression associated with virulence or to compare biological profiles of wild type and mutant strains. Here, we employed DNA microarrays to study the early stages of the infection process (24, 72 and 120 h post-inoculation) of Xanthomonas citri subsp. citri (Xac) infecting Citrus sinensis to interrogate the expression profiles of hypothetical genes. Results: Under infective conditions, 446 genes were up- and 306 downregulated. Outstanding among genes upregulated during infection were those involved in synthesizing the Type 3 Secretion System and effectors, xanthan gum and quorum-sensing induction, and flagellum synthesis and regulation. Additionally, 161 hypothetical genes were up- and 100 were downregulated, 49 of which are known to have a significant biological role. To understand hypothetical gene co-regulation or -expression, nine expression profiles including 158 genes were identified during the three infection phases. Of these, 47 hypothetical genes were identified as having expression profiles associated with at least one connected to a gene associated with adaptation and virulence. Conclusions: Expression patterns of six differentially expressed genes were validated by quantitative reverse transcription polymerase chain reaction, thus demonstrating the effectiveness of this tool in global gene expression analysis in Xac. |
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Laia, Marcelo Luiz deMoreira, Leandro MarcioGonçalves, Janaína FernandesFerro, Maria Inês TiraboschiRodrigues, Any Caroliny PintoSantos, Jéssica Naiara dosFelestrino, Érica BarbosaFerro, Jesus Aparecido2020-04-06T16:07:55Z2020-04-06T16:07:55Z2019LAIA, M. L. et al. Gene expression analysis identifies hypothetical genes that may be critical during the infection process of Xanthomonas citri subsp. citri. Electronic Journal of Biotechnology, v. 42, p. 30-41, nov. 2019. Disponível em: <https://www.sciencedirect.com/science/article/pii/S0717345819300430#!>. Acesso em: 10 fev. 2020.0717-3458http://www.repositorio.ufop.br/handle/123456789/12030https://doi.org/10.1016/j.ejbt.2019.10.003Background: Gene expression analysis via microarray is widely used in phytobacteria to validate differential gene expression associated with virulence or to compare biological profiles of wild type and mutant strains. Here, we employed DNA microarrays to study the early stages of the infection process (24, 72 and 120 h post-inoculation) of Xanthomonas citri subsp. citri (Xac) infecting Citrus sinensis to interrogate the expression profiles of hypothetical genes. Results: Under infective conditions, 446 genes were up- and 306 downregulated. Outstanding among genes upregulated during infection were those involved in synthesizing the Type 3 Secretion System and effectors, xanthan gum and quorum-sensing induction, and flagellum synthesis and regulation. Additionally, 161 hypothetical genes were up- and 100 were downregulated, 49 of which are known to have a significant biological role. To understand hypothetical gene co-regulation or -expression, nine expression profiles including 158 genes were identified during the three infection phases. Of these, 47 hypothetical genes were identified as having expression profiles associated with at least one connected to a gene associated with adaptation and virulence. Conclusions: Expression patterns of six differentially expressed genes were validated by quantitative reverse transcription polymerase chain reaction, thus demonstrating the effectiveness of this tool in global gene expression analysis in Xac.This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). Fonte: o próprio artigoinfo:eu-repo/semantics/openAccessDNA microarraysHypothetical genesOligonucleotide array sequence analysisRT-qPCRTranscriptomeGene expression analysis identifies hypothetical genes that may be critical during the infection process of Xanthomonas citri subsp. citri.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleengreponame:Repositório Institucional da UFOPinstname:Universidade Federal de Ouro Preto (UFOP)instacron:UFOPLICENSElicense.txtlicense.txttext/plain; charset=utf-8924http://www.repositorio.ufop.br/bitstream/123456789/12030/2/license.txt62604f8d955274beb56c80ce1ee5dcaeMD52ORIGINALARTIGO_GeneExpressionAnalysis.pdfARTIGO_GeneExpressionAnalysis.pdfapplication/pdf2135410http://www.repositorio.ufop.br/bitstream/123456789/12030/1/ARTIGO_GeneExpressionAnalysis.pdf52c1b69288d5354c20f154acaad052adMD51123456789/120302020-04-06 12:08:29.071oai:localhost: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ório InstitucionalPUBhttp://www.repositorio.ufop.br/oai/requestrepositorio@ufop.edu.bropendoar:32332020-04-06T16:08:29Repositório Institucional da UFOP - Universidade Federal de Ouro Preto (UFOP)false |
dc.title.pt_BR.fl_str_mv |
Gene expression analysis identifies hypothetical genes that may be critical during the infection process of Xanthomonas citri subsp. citri. |
title |
Gene expression analysis identifies hypothetical genes that may be critical during the infection process of Xanthomonas citri subsp. citri. |
spellingShingle |
Gene expression analysis identifies hypothetical genes that may be critical during the infection process of Xanthomonas citri subsp. citri. Laia, Marcelo Luiz de DNA microarrays Hypothetical genes Oligonucleotide array sequence analysis RT-qPCR Transcriptome |
title_short |
Gene expression analysis identifies hypothetical genes that may be critical during the infection process of Xanthomonas citri subsp. citri. |
title_full |
Gene expression analysis identifies hypothetical genes that may be critical during the infection process of Xanthomonas citri subsp. citri. |
title_fullStr |
Gene expression analysis identifies hypothetical genes that may be critical during the infection process of Xanthomonas citri subsp. citri. |
title_full_unstemmed |
Gene expression analysis identifies hypothetical genes that may be critical during the infection process of Xanthomonas citri subsp. citri. |
title_sort |
Gene expression analysis identifies hypothetical genes that may be critical during the infection process of Xanthomonas citri subsp. citri. |
author |
Laia, Marcelo Luiz de |
author_facet |
Laia, Marcelo Luiz de Moreira, Leandro Marcio Gonçalves, Janaína Fernandes Ferro, Maria Inês Tiraboschi Rodrigues, Any Caroliny Pinto Santos, Jéssica Naiara dos Felestrino, Érica Barbosa Ferro, Jesus Aparecido |
author_role |
author |
author2 |
Moreira, Leandro Marcio Gonçalves, Janaína Fernandes Ferro, Maria Inês Tiraboschi Rodrigues, Any Caroliny Pinto Santos, Jéssica Naiara dos Felestrino, Érica Barbosa Ferro, Jesus Aparecido |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Laia, Marcelo Luiz de Moreira, Leandro Marcio Gonçalves, Janaína Fernandes Ferro, Maria Inês Tiraboschi Rodrigues, Any Caroliny Pinto Santos, Jéssica Naiara dos Felestrino, Érica Barbosa Ferro, Jesus Aparecido |
dc.subject.por.fl_str_mv |
DNA microarrays Hypothetical genes Oligonucleotide array sequence analysis RT-qPCR Transcriptome |
topic |
DNA microarrays Hypothetical genes Oligonucleotide array sequence analysis RT-qPCR Transcriptome |
description |
Background: Gene expression analysis via microarray is widely used in phytobacteria to validate differential gene expression associated with virulence or to compare biological profiles of wild type and mutant strains. Here, we employed DNA microarrays to study the early stages of the infection process (24, 72 and 120 h post-inoculation) of Xanthomonas citri subsp. citri (Xac) infecting Citrus sinensis to interrogate the expression profiles of hypothetical genes. Results: Under infective conditions, 446 genes were up- and 306 downregulated. Outstanding among genes upregulated during infection were those involved in synthesizing the Type 3 Secretion System and effectors, xanthan gum and quorum-sensing induction, and flagellum synthesis and regulation. Additionally, 161 hypothetical genes were up- and 100 were downregulated, 49 of which are known to have a significant biological role. To understand hypothetical gene co-regulation or -expression, nine expression profiles including 158 genes were identified during the three infection phases. Of these, 47 hypothetical genes were identified as having expression profiles associated with at least one connected to a gene associated with adaptation and virulence. Conclusions: Expression patterns of six differentially expressed genes were validated by quantitative reverse transcription polymerase chain reaction, thus demonstrating the effectiveness of this tool in global gene expression analysis in Xac. |
publishDate |
2019 |
dc.date.issued.fl_str_mv |
2019 |
dc.date.accessioned.fl_str_mv |
2020-04-06T16:07:55Z |
dc.date.available.fl_str_mv |
2020-04-06T16:07:55Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
LAIA, M. L. et al. Gene expression analysis identifies hypothetical genes that may be critical during the infection process of Xanthomonas citri subsp. citri. Electronic Journal of Biotechnology, v. 42, p. 30-41, nov. 2019. Disponível em: <https://www.sciencedirect.com/science/article/pii/S0717345819300430#!>. Acesso em: 10 fev. 2020. |
dc.identifier.uri.fl_str_mv |
http://www.repositorio.ufop.br/handle/123456789/12030 |
dc.identifier.issn.none.fl_str_mv |
0717-3458 |
dc.identifier.doi.pt_BR.fl_str_mv |
https://doi.org/10.1016/j.ejbt.2019.10.003 |
identifier_str_mv |
LAIA, M. L. et al. Gene expression analysis identifies hypothetical genes that may be critical during the infection process of Xanthomonas citri subsp. citri. Electronic Journal of Biotechnology, v. 42, p. 30-41, nov. 2019. Disponível em: <https://www.sciencedirect.com/science/article/pii/S0717345819300430#!>. Acesso em: 10 fev. 2020. 0717-3458 |
url |
http://www.repositorio.ufop.br/handle/123456789/12030 https://doi.org/10.1016/j.ejbt.2019.10.003 |
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eng |
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eng |
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info:eu-repo/semantics/openAccess |
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openAccess |
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reponame:Repositório Institucional da UFOP instname:Universidade Federal de Ouro Preto (UFOP) instacron:UFOP |
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