Exoproteome and Secretome Derived Broad Spectrum Novel Drug and Vaccine Candidates in Vibrio cholerae Targeted by Piper betel Derived Compounds
Autor(a) principal: | |
---|---|
Data de Publicação: | 2013 |
Outros Autores: | , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFPA |
Texto Completo: | https://repositorio.ufpa.br/jspui/handle/2011/15631 |
Resumo: | Vibrio cholerae is the causal organism of the cholera epidemic, which is mostly prevalent in developing and underdeveloped countries. However, incidences of cholera in developed countries are also alarming. Because of the emergence of new drug resistant strains, even though several generic drugs and vaccines have been developed over time, Vibrio infections remain a global health problem that appeals for the development of novel drugs and vaccines against the pathogen. Here, applying comparative proteomic and reverse vaccinology approaches to the exoproteome and secretome of the pathogen, we have identified three candidate targets (ompU, uppP and yajC) for most of the pathogenic Vibrio strains. Two targets (uppP and yajC) are novel to Vibrio, and two targets (uppP and ompU) can be used to develop both drugs and vaccines (dual targets) against broad spectrum Vibrio serotypes. Using our novel computational approach, we have identified three peptide vaccine candidates that have high potential to induce both B- and T-cell-mediated immune responses from our identified two dual targets. These two targets were modeled and subjected to virtual screening against natural compounds derived from Piper betel. Seven compounds were identified first time from Piper betel to be highly effective to render the function of these targets to identify them as emerging potential drugs against Vibrio. Our preliminary validation suggests that these identified peptide vaccines and betel compounds are highly effective against Vibrio cholerae. Currently we are exhaustively validating these targets, candidate peptide vaccines, and betel derived lead compounds against a number of Vibrio species. |
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2023-05-30T13:16:33Z2023-05-30T13:16:33Z2013-01BARH, Debmalya et al. Exoproteome and Secretome Derived Broad Spectrum Novel Drug and Vaccine Candidates in Vibrio cholerae Targeted by Piper betel Derived Compounds. PLoS ONE, online, v. 8, n. 1, e52773, Jan. 2013. DOI: https://doi.org/10.1371/journal.pone.0052773. Disponível em: http://repositorio.ufpa.br/jspui/handle/2011/15631. Acesso em:.1932-6203https://repositorio.ufpa.br/jspui/handle/2011/1563110.1371/journal.pone.0052773Vibrio cholerae is the causal organism of the cholera epidemic, which is mostly prevalent in developing and underdeveloped countries. However, incidences of cholera in developed countries are also alarming. Because of the emergence of new drug resistant strains, even though several generic drugs and vaccines have been developed over time, Vibrio infections remain a global health problem that appeals for the development of novel drugs and vaccines against the pathogen. Here, applying comparative proteomic and reverse vaccinology approaches to the exoproteome and secretome of the pathogen, we have identified three candidate targets (ompU, uppP and yajC) for most of the pathogenic Vibrio strains. Two targets (uppP and yajC) are novel to Vibrio, and two targets (uppP and ompU) can be used to develop both drugs and vaccines (dual targets) against broad spectrum Vibrio serotypes. Using our novel computational approach, we have identified three peptide vaccine candidates that have high potential to induce both B- and T-cell-mediated immune responses from our identified two dual targets. These two targets were modeled and subjected to virtual screening against natural compounds derived from Piper betel. Seven compounds were identified first time from Piper betel to be highly effective to render the function of these targets to identify them as emerging potential drugs against Vibrio. Our preliminary validation suggests that these identified peptide vaccines and betel compounds are highly effective against Vibrio cholerae. Currently we are exhaustively validating these targets, candidate peptide vaccines, and betel derived lead compounds against a number of Vibrio species.RAMOS, R. T. J.; CARNEIRO, A. R.; SILVA, A. L. C. Universidade Federal do ParáengPublic Library of SciencePLOSEstados unidosPLoS ONEhttp://creativecommons.org/licenses/by-nc-nd/3.0/br/info:eu-repo/semantics/openAccesshttps://journals.plos.org/plosone/article?id=10.1371/journal.pone.0052773reponame:Repositório Institucional da UFPAinstname:Universidade Federal do Pará (UFPA)instacron:UFPACorynebacterium pseudotuberculosisExoproteome and Secretome Derived Broad Spectrum Novel Drug and Vaccine Candidates in Vibrio cholerae Targeted by Piper betel Derived Compoundsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article81e52773http://lattes.cnpq.br/9326996169562214http://lattes.cnpq.br/http://lattes.cnpq.br/http://lattes.cnpq.br/http://lattes.cnpq.br/http://lattes.cnpq.br/2419194183390541http://lattes.cnpq.br/http://lattes.cnpq.br/http://lattes.cnpq.br/3752226356973936http://lattes.cnpq.br/2882537281050009http://lattes.cnpq.br/6389878370640685http://lattes.cnpq.br/1274395392752454http://lattes.cnpq.br/2484200467965399http://lattes.cnpq.br/7533716053525477http://lattes.cnpq.br/4393381414254469http://lattes.cnpq.br/1462921724686345http://lattes.cnpq.br/9198272608157135http://lattes.cnpq.br/7642043789034070http://lattes.cnpq.br/http://lattes.cnpq.br/http://lattes.cnpq.br/http://lattes.cnpq.br/http://lattes.cnpq.br/1020477751003832BARH, DebmalyaSHARMA, Neha BarveGUPTA, KrishnakantCHANDRA, SudhaJAIN, NehaTIWARI, SandeepLEON-SICAIROS, NidiaCANIZALEZ-ROMAN, AdrianSANTOS, Anderson Rodrigues dosHASSAN, Syed ShahALMEIDA, Sintia Silva deRAMOS, Rommel Thiago JucaABREU, Vinicius Augusto Carvalho deCARNEIRO, Adriana RibeiroSOARES, Siomar de CastroCASTRO, Thiago Luiz de PaulaMIYOSHI, AndersonSILVA, Artur Luiz da Costa daKUMAR, AnilNARAYAN MISRA, AmarendraBLUM, KennethBRAVERMAN, Eric R.AZEVEDO, Vasco Ariston de Carvalhohttps://orcid.orghttps://orcid.orghttps://orcid.orghttps://orcid.orghttps://orcid.orghttps://orcid.org/0000-0002-8554-1660https://orcid.orghttps://orcid.orghttps://orcid.org/0000-0003-3418-0823https://orcid.orghttps://orcid.org/0000-0003-0270-9059https://orcid.org/0000-0002-8032-1474https://orcid.orghttps://orcid.org/0000-0003-1522-9871https://orcid.org/0000-0001-7299-3724https://orcid.org/0000-0002-2869-5065https://orcid.orghttps://orcid.orghttps://orcid.orghttps://orcid.orghttps://orcid.orghttps://orcid.orghttps://orcid.org/0000-0002-4775-2280ORIGINALArticle_ExoproteomeSecretomeDerived.pdfArticle_ExoproteomeSecretomeDerived.pdfapplication/pdf666833http://repositorio.ufpa.br/oai/bitstream/2011/15631/1/Article_ExoproteomeSecretomeDerived.pdf6aefc5ad3fc1898d0548bc456f8c1801MD51CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; 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dc.title.pt_BR.fl_str_mv |
Exoproteome and Secretome Derived Broad Spectrum Novel Drug and Vaccine Candidates in Vibrio cholerae Targeted by Piper betel Derived Compounds |
title |
Exoproteome and Secretome Derived Broad Spectrum Novel Drug and Vaccine Candidates in Vibrio cholerae Targeted by Piper betel Derived Compounds |
spellingShingle |
Exoproteome and Secretome Derived Broad Spectrum Novel Drug and Vaccine Candidates in Vibrio cholerae Targeted by Piper betel Derived Compounds BARH, Debmalya Corynebacterium pseudotuberculosis |
title_short |
Exoproteome and Secretome Derived Broad Spectrum Novel Drug and Vaccine Candidates in Vibrio cholerae Targeted by Piper betel Derived Compounds |
title_full |
Exoproteome and Secretome Derived Broad Spectrum Novel Drug and Vaccine Candidates in Vibrio cholerae Targeted by Piper betel Derived Compounds |
title_fullStr |
Exoproteome and Secretome Derived Broad Spectrum Novel Drug and Vaccine Candidates in Vibrio cholerae Targeted by Piper betel Derived Compounds |
title_full_unstemmed |
Exoproteome and Secretome Derived Broad Spectrum Novel Drug and Vaccine Candidates in Vibrio cholerae Targeted by Piper betel Derived Compounds |
title_sort |
Exoproteome and Secretome Derived Broad Spectrum Novel Drug and Vaccine Candidates in Vibrio cholerae Targeted by Piper betel Derived Compounds |
author |
BARH, Debmalya |
author_facet |
BARH, Debmalya SHARMA, Neha Barve GUPTA, Krishnakant CHANDRA, Sudha JAIN, Neha TIWARI, Sandeep LEON-SICAIROS, Nidia CANIZALEZ-ROMAN, Adrian SANTOS, Anderson Rodrigues dos HASSAN, Syed Shah ALMEIDA, Sintia Silva de RAMOS, Rommel Thiago Juca ABREU, Vinicius Augusto Carvalho de CARNEIRO, Adriana Ribeiro SOARES, Siomar de Castro CASTRO, Thiago Luiz de Paula MIYOSHI, Anderson SILVA, Artur Luiz da Costa da KUMAR, Anil NARAYAN MISRA, Amarendra BLUM, Kenneth BRAVERMAN, Eric R. AZEVEDO, Vasco Ariston de Carvalho https://orcid.org https://orcid.org/0000-0002-8554-1660 https://orcid.org/0000-0003-3418-0823 https://orcid.org/0000-0003-0270-9059 https://orcid.org/0000-0002-8032-1474 https://orcid.org/0000-0003-1522-9871 https://orcid.org/0000-0001-7299-3724 https://orcid.org/0000-0002-2869-5065 https://orcid.org/0000-0002-4775-2280 |
author_role |
author |
author2 |
SHARMA, Neha Barve GUPTA, Krishnakant CHANDRA, Sudha JAIN, Neha TIWARI, Sandeep LEON-SICAIROS, Nidia CANIZALEZ-ROMAN, Adrian SANTOS, Anderson Rodrigues dos HASSAN, Syed Shah ALMEIDA, Sintia Silva de RAMOS, Rommel Thiago Juca ABREU, Vinicius Augusto Carvalho de CARNEIRO, Adriana Ribeiro SOARES, Siomar de Castro CASTRO, Thiago Luiz de Paula MIYOSHI, Anderson SILVA, Artur Luiz da Costa da KUMAR, Anil NARAYAN MISRA, Amarendra BLUM, Kenneth BRAVERMAN, Eric R. AZEVEDO, Vasco Ariston de Carvalho https://orcid.org https://orcid.org/0000-0002-8554-1660 https://orcid.org/0000-0003-3418-0823 https://orcid.org/0000-0003-0270-9059 https://orcid.org/0000-0002-8032-1474 https://orcid.org/0000-0003-1522-9871 https://orcid.org/0000-0001-7299-3724 https://orcid.org/0000-0002-2869-5065 https://orcid.org/0000-0002-4775-2280 |
author2_role |
author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/9326996169562214 http://lattes.cnpq.br/ http://lattes.cnpq.br/ http://lattes.cnpq.br/ http://lattes.cnpq.br/ http://lattes.cnpq.br/2419194183390541 http://lattes.cnpq.br/ http://lattes.cnpq.br/ http://lattes.cnpq.br/3752226356973936 http://lattes.cnpq.br/2882537281050009 http://lattes.cnpq.br/6389878370640685 http://lattes.cnpq.br/1274395392752454 http://lattes.cnpq.br/2484200467965399 http://lattes.cnpq.br/7533716053525477 http://lattes.cnpq.br/4393381414254469 http://lattes.cnpq.br/1462921724686345 http://lattes.cnpq.br/9198272608157135 http://lattes.cnpq.br/7642043789034070 http://lattes.cnpq.br/ http://lattes.cnpq.br/ http://lattes.cnpq.br/ http://lattes.cnpq.br/ http://lattes.cnpq.br/1020477751003832 |
dc.contributor.author.fl_str_mv |
BARH, Debmalya SHARMA, Neha Barve GUPTA, Krishnakant CHANDRA, Sudha JAIN, Neha TIWARI, Sandeep LEON-SICAIROS, Nidia CANIZALEZ-ROMAN, Adrian SANTOS, Anderson Rodrigues dos HASSAN, Syed Shah ALMEIDA, Sintia Silva de RAMOS, Rommel Thiago Juca ABREU, Vinicius Augusto Carvalho de CARNEIRO, Adriana Ribeiro SOARES, Siomar de Castro CASTRO, Thiago Luiz de Paula MIYOSHI, Anderson SILVA, Artur Luiz da Costa da KUMAR, Anil NARAYAN MISRA, Amarendra BLUM, Kenneth BRAVERMAN, Eric R. AZEVEDO, Vasco Ariston de Carvalho https://orcid.org https://orcid.org https://orcid.org https://orcid.org https://orcid.org https://orcid.org/0000-0002-8554-1660 https://orcid.org https://orcid.org https://orcid.org/0000-0003-3418-0823 https://orcid.org https://orcid.org/0000-0003-0270-9059 https://orcid.org/0000-0002-8032-1474 https://orcid.org https://orcid.org/0000-0003-1522-9871 https://orcid.org/0000-0001-7299-3724 https://orcid.org/0000-0002-2869-5065 https://orcid.org https://orcid.org https://orcid.org https://orcid.org https://orcid.org https://orcid.org https://orcid.org/0000-0002-4775-2280 |
dc.subject.por.fl_str_mv |
Corynebacterium pseudotuberculosis |
topic |
Corynebacterium pseudotuberculosis |
description |
Vibrio cholerae is the causal organism of the cholera epidemic, which is mostly prevalent in developing and underdeveloped countries. However, incidences of cholera in developed countries are also alarming. Because of the emergence of new drug resistant strains, even though several generic drugs and vaccines have been developed over time, Vibrio infections remain a global health problem that appeals for the development of novel drugs and vaccines against the pathogen. Here, applying comparative proteomic and reverse vaccinology approaches to the exoproteome and secretome of the pathogen, we have identified three candidate targets (ompU, uppP and yajC) for most of the pathogenic Vibrio strains. Two targets (uppP and yajC) are novel to Vibrio, and two targets (uppP and ompU) can be used to develop both drugs and vaccines (dual targets) against broad spectrum Vibrio serotypes. Using our novel computational approach, we have identified three peptide vaccine candidates that have high potential to induce both B- and T-cell-mediated immune responses from our identified two dual targets. These two targets were modeled and subjected to virtual screening against natural compounds derived from Piper betel. Seven compounds were identified first time from Piper betel to be highly effective to render the function of these targets to identify them as emerging potential drugs against Vibrio. Our preliminary validation suggests that these identified peptide vaccines and betel compounds are highly effective against Vibrio cholerae. Currently we are exhaustively validating these targets, candidate peptide vaccines, and betel derived lead compounds against a number of Vibrio species. |
publishDate |
2013 |
dc.date.issued.fl_str_mv |
2013-01 |
dc.date.accessioned.fl_str_mv |
2023-05-30T13:16:33Z |
dc.date.available.fl_str_mv |
2023-05-30T13:16:33Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
BARH, Debmalya et al. Exoproteome and Secretome Derived Broad Spectrum Novel Drug and Vaccine Candidates in Vibrio cholerae Targeted by Piper betel Derived Compounds. PLoS ONE, online, v. 8, n. 1, e52773, Jan. 2013. DOI: https://doi.org/10.1371/journal.pone.0052773. Disponível em: http://repositorio.ufpa.br/jspui/handle/2011/15631. Acesso em:. |
dc.identifier.uri.fl_str_mv |
https://repositorio.ufpa.br/jspui/handle/2011/15631 |
dc.identifier.issn.pt_BR.fl_str_mv |
1932-6203 |
dc.identifier.doi.pt_BR.fl_str_mv |
10.1371/journal.pone.0052773 |
identifier_str_mv |
BARH, Debmalya et al. Exoproteome and Secretome Derived Broad Spectrum Novel Drug and Vaccine Candidates in Vibrio cholerae Targeted by Piper betel Derived Compounds. PLoS ONE, online, v. 8, n. 1, e52773, Jan. 2013. DOI: https://doi.org/10.1371/journal.pone.0052773. Disponível em: http://repositorio.ufpa.br/jspui/handle/2011/15631. Acesso em:. 1932-6203 10.1371/journal.pone.0052773 |
url |
https://repositorio.ufpa.br/jspui/handle/2011/15631 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.pt_BR.fl_str_mv |
PLoS ONE |
dc.rights.driver.fl_str_mv |
http://creativecommons.org/licenses/by-nc-nd/3.0/br/ info:eu-repo/semantics/openAccess |
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http://creativecommons.org/licenses/by-nc-nd/3.0/br/ |
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openAccess |
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Public Library of Science |
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