Caracterização de grupos agr e sua relação com perfil enterotoxigênico e antimicrobiano em Staphylococcus aureus isolados de diferentes origens.
Autor(a) principal: | |
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Data de Publicação: | 2009 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFPel - Guaiaca |
Texto Completo: | http://repositorio.ufpel.edu.br/handle/ri/1312 |
Resumo: | he accessory gene regulator (agr) is a S. aureus global regulator of virulence factors, as the staphylococcal enterotoxins (SE), responsible for the staphylococcal food poisoning. There are four different agr groups due to the polymorphism in the amino acids sequence of the agrC and agrD. In the literature is described a relationship among agr groups and virulence factors, diseases, preferential host and antibiotic resistance. In this context, it was aimed to characterize the agr groups through biplex PCR, and relationship among agr groups, enterotoxigenic and antimicrobian profiles of S. aureus isolated from foods and bovine mastitis milk. A total of 115 strains were used to characterize the agr groups , being 30 isolated from f bovine mastitis milk and 85 isolated from several sources of foods. To assess the relationship between agr groups and enterotoxin production were used 14/85 strains previously characterized for the presence of some enterotoxins (sea, seb, sec, sed e cluster egc). To determine the profile of antibiotics resistance were used 71/115 strains. We observed a prevalence of agr group II with19.1% (22/115 strains), followed by the agr I with 8.6% (10/115 strains), agr III with 7.8% (9 / 115 strains), and agr IV with6.0% (7 / 115 strains). Among the strains isolated from bovine mastitis milk agr group I was prevailed with 20% (6/30 strains), whereas in the strains isolated from several food sources was observed prevalence of agr group II with 32.7% (18/85 strains), especially among those from chicken meat. Among the 14 strains (14/85) that contained enterotoxin genes, the majority of them contained the cluster egc (70%) belonged to agr II, whereas no relationship was found with those who had the genes for the classical SE (sea, seb, sec, sed). Considering the antibiotic resistance 100% of bovine mastitis milk strains and from various sources of food were resistant to penicillin, ampicillin, cefoxitin, and vancomycin. Relationship was observed between food strains, which were resistant to vancomycin and agr II, however, no relationship was found between antibiotic profile and agr groups among the strains isolated from bovine mastitis milk. These results demonstrated the prevalence of agr II among food strains and agr I among bovine mastitis milk strains. Moreover, the strains that carried the cluster egc were predominant agr II, which could indicate the occurrence of a clonal group among those. Another important result obtained in this study was the high rate of S. aureus multiresistant strains isolated from food, which emphasizes the importance of dissemination of these strains among foods. |
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http://lattes.cnpq.br/3212309753624070http://lattes.cnpq.br/5231261744494804Silva, Wladimir Padilha daBassani, Milena Tomasi2014-08-20T13:42:05Z2011-05-182014-08-20T13:42:05Z2009-10-27BASSANI, Milena Tomasi. Caracterização de grupos agr e sua relação com perfil enterotoxigênico e antimicrobiano em Staphylococcus aureus isolados de diferentes origens.. 2009. 71 f. Dissertação (Mestrado em Agronomia) - Universidade Federal de Pelotas, Pelotas, 2009.http://repositorio.ufpel.edu.br/handle/ri/1312he accessory gene regulator (agr) is a S. aureus global regulator of virulence factors, as the staphylococcal enterotoxins (SE), responsible for the staphylococcal food poisoning. There are four different agr groups due to the polymorphism in the amino acids sequence of the agrC and agrD. In the literature is described a relationship among agr groups and virulence factors, diseases, preferential host and antibiotic resistance. In this context, it was aimed to characterize the agr groups through biplex PCR, and relationship among agr groups, enterotoxigenic and antimicrobian profiles of S. aureus isolated from foods and bovine mastitis milk. A total of 115 strains were used to characterize the agr groups , being 30 isolated from f bovine mastitis milk and 85 isolated from several sources of foods. To assess the relationship between agr groups and enterotoxin production were used 14/85 strains previously characterized for the presence of some enterotoxins (sea, seb, sec, sed e cluster egc). To determine the profile of antibiotics resistance were used 71/115 strains. We observed a prevalence of agr group II with19.1% (22/115 strains), followed by the agr I with 8.6% (10/115 strains), agr III with 7.8% (9 / 115 strains), and agr IV with6.0% (7 / 115 strains). Among the strains isolated from bovine mastitis milk agr group I was prevailed with 20% (6/30 strains), whereas in the strains isolated from several food sources was observed prevalence of agr group II with 32.7% (18/85 strains), especially among those from chicken meat. Among the 14 strains (14/85) that contained enterotoxin genes, the majority of them contained the cluster egc (70%) belonged to agr II, whereas no relationship was found with those who had the genes for the classical SE (sea, seb, sec, sed). Considering the antibiotic resistance 100% of bovine mastitis milk strains and from various sources of food were resistant to penicillin, ampicillin, cefoxitin, and vancomycin. Relationship was observed between food strains, which were resistant to vancomycin and agr II, however, no relationship was found between antibiotic profile and agr groups among the strains isolated from bovine mastitis milk. These results demonstrated the prevalence of agr II among food strains and agr I among bovine mastitis milk strains. Moreover, the strains that carried the cluster egc were predominant agr II, which could indicate the occurrence of a clonal group among those. Another important result obtained in this study was the high rate of S. aureus multiresistant strains isolated from food, which emphasizes the importance of dissemination of these strains among foods.O accessory gene regulator (agr) é um regulador global de fatores de virulência em S. aureus, como as enterotoxinas estafilocócicas (EE), responsáveis pela intoxicação alimentar estafilocócica. São conhecidos quatro distintos grupos agr devido ao polimorfismo na seqüência dos aminoácidos de agrC e agrD. Na literatura descreve-se relação entre fatores de virulência, patogenias, hospedeiro preferencial e perfil de resistência a antibióticos com grupos agr. Neste contexto, objetivou-se caracterizar grupos agr através de biplex PCR, e relacioná-los com os perfis enterotoxigênico e antimicrobiano de cepas de S. aureus isoladas em alimentos e leite de vacas mastíticas. Para caracterização dos grupos agr foram utilizadas 115 cepas, sendo 30 isoladas em leite de vacas mastíticas e 85 em diversas fontes de alimentos. Para a relação entre grupos agr e produção de enterotoxina foram utilizadas 14/85 cepas previamente caracterizadas quanto à presença de alguma enterotoxina (eea, eeb, eec, eed e cluster egc), já para determinar o perfil de resistência a antibióticos utilizaram-se 71/115 cepas. Observou-se uma prevalência do grupo agr II, com 19,1% (22/115 cepas), seguido do agr I, com 8,6% (10/115 cepas), agr III 7,8% (9/115 cepas), e agr IV, 6,0% (7/115 cepas). Entre as cepas isoladas em leite de vacas com mastite houve predomínio do grupo agr I, com 20% (6/30 cepas); já nas cepas isoladas de diversas fontes de alimentos observou-se prevalência do grupo agr II, com 32,7% (18/85 cepas), especialmente entre as provenientes de carne de frango. Entre as 14/85 cepas que carreavam genes de enterotoxinas, a maioria que albergava o cluster egc (70%), pertencia ao grupo agr II, enquanto nenhuma relação foi observada com aquelas que possuíam os genes para as EE clássicas (eea, eeb, eec, eed). Com relação ao perfil de resistência antimicrobiana, 100% das cepas isoladas de leite de vacas com mastite e das diversas fontes de alimentos apresentaram resistência à penicilina, ampicilina, cefoxitina e vancomicina. Observou-se relação entre cepas isoladas de alimentos, que eram resistentes à vancomicina e grupo agr II, entretanto, nenhuma relação foi observada entre perfil antimicrobiano e grupos agr entre as cepas isoladas em leite de vacas com mastite. Através destes resultados demonstra-se a prevalência do grupo agr II entre as cepas isoladas de alimentos e do grupo agr I em cepas isoladas de leite de vacas mastíticas. Além disso, nas cepas que carreiam o cluster egc houve predominância do grupo agr II, podendo indicar um grupo clonal entre essas. Outro resultado relevante obtido neste estudo foi à elevada taxa de cepas de S. aureus multiresistentes isoladas em alimentos, o que ressalta a importância da disseminação de cepas multiresistentes entre os alimentos.application/pdfporUniversidade Federal de PelotasPrograma de Pós-Graduação em Ciência e Tecnologia AgroindustrialUFPelBRFaculdade de Agronomia Eliseu MacielAccessory gene regulatorStaphylococcus aureusEnterotoxinasCepas multiresistentesAccessory gene regulatorStaphylococcus aureusEnterotoxinMultiresistant strainsCNPQ::CIENCIAS AGRARIAS::CIENCIA E TECNOLOGIA DE ALIMENTOS::TECNOLOGIA DE ALIMENTOSCaracterização de grupos agr e sua relação com perfil enterotoxigênico e antimicrobiano em Staphylococcus aureus isolados de diferentes origens.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFPel - Guaiacainstname:Universidade Federal de Pelotas (UFPEL)instacron:UFPELORIGINALDissertacao_Milena_Tomasi_ Bassani.pdfapplication/pdf628517http://guaiaca.ufpel.edu.br/xmlui/bitstream/123456789/1312/1/Dissertacao_Milena_Tomasi_%20Bassani.pdfaf4a756ae84e13ef34c451b2d486162eMD51open accessTEXTDissertacao_Milena_Tomasi_ Bassani.pdf.txtDissertacao_Milena_Tomasi_ Bassani.pdf.txtExtracted Texttext/plain123770http://guaiaca.ufpel.edu.br/xmlui/bitstream/123456789/1312/2/Dissertacao_Milena_Tomasi_%20Bassani.pdf.txt6229192140bba767a0a93f5124238c31MD52open accessTHUMBNAILDissertacao_Milena_Tomasi_ Bassani.pdf.jpgDissertacao_Milena_Tomasi_ Bassani.pdf.jpgGenerated Thumbnailimage/jpeg1316http://guaiaca.ufpel.edu.br/xmlui/bitstream/123456789/1312/3/Dissertacao_Milena_Tomasi_%20Bassani.pdf.jpg730d4cf5e7df32a508de7beb806813d3MD53open access123456789/13122022-12-14 18:54:23.236open accessoai:guaiaca.ufpel.edu.br:123456789/1312Repositório InstitucionalPUBhttp://repositorio.ufpel.edu.br/oai/requestrippel@ufpel.edu.br || repositorio@ufpel.edu.br || aline.batista@ufpel.edu.bropendoar:2022-12-14T21:54:23Repositório Institucional da UFPel - Guaiaca - Universidade Federal de Pelotas (UFPEL)false |
dc.title.por.fl_str_mv |
Caracterização de grupos agr e sua relação com perfil enterotoxigênico e antimicrobiano em Staphylococcus aureus isolados de diferentes origens. |
title |
Caracterização de grupos agr e sua relação com perfil enterotoxigênico e antimicrobiano em Staphylococcus aureus isolados de diferentes origens. |
spellingShingle |
Caracterização de grupos agr e sua relação com perfil enterotoxigênico e antimicrobiano em Staphylococcus aureus isolados de diferentes origens. Bassani, Milena Tomasi Accessory gene regulator Staphylococcus aureus Enterotoxinas Cepas multiresistentes Accessory gene regulator Staphylococcus aureus Enterotoxin Multiresistant strains CNPQ::CIENCIAS AGRARIAS::CIENCIA E TECNOLOGIA DE ALIMENTOS::TECNOLOGIA DE ALIMENTOS |
title_short |
Caracterização de grupos agr e sua relação com perfil enterotoxigênico e antimicrobiano em Staphylococcus aureus isolados de diferentes origens. |
title_full |
Caracterização de grupos agr e sua relação com perfil enterotoxigênico e antimicrobiano em Staphylococcus aureus isolados de diferentes origens. |
title_fullStr |
Caracterização de grupos agr e sua relação com perfil enterotoxigênico e antimicrobiano em Staphylococcus aureus isolados de diferentes origens. |
title_full_unstemmed |
Caracterização de grupos agr e sua relação com perfil enterotoxigênico e antimicrobiano em Staphylococcus aureus isolados de diferentes origens. |
title_sort |
Caracterização de grupos agr e sua relação com perfil enterotoxigênico e antimicrobiano em Staphylococcus aureus isolados de diferentes origens. |
author |
Bassani, Milena Tomasi |
author_facet |
Bassani, Milena Tomasi |
author_role |
author |
dc.contributor.authorLattes.por.fl_str_mv |
http://lattes.cnpq.br/3212309753624070 |
dc.contributor.advisorLattes.por.fl_str_mv |
http://lattes.cnpq.br/5231261744494804 |
dc.contributor.advisor1.fl_str_mv |
Silva, Wladimir Padilha da |
dc.contributor.author.fl_str_mv |
Bassani, Milena Tomasi |
contributor_str_mv |
Silva, Wladimir Padilha da |
dc.subject.por.fl_str_mv |
Accessory gene regulator Staphylococcus aureus Enterotoxinas Cepas multiresistentes |
topic |
Accessory gene regulator Staphylococcus aureus Enterotoxinas Cepas multiresistentes Accessory gene regulator Staphylococcus aureus Enterotoxin Multiresistant strains CNPQ::CIENCIAS AGRARIAS::CIENCIA E TECNOLOGIA DE ALIMENTOS::TECNOLOGIA DE ALIMENTOS |
dc.subject.eng.fl_str_mv |
Accessory gene regulator Staphylococcus aureus Enterotoxin Multiresistant strains |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS AGRARIAS::CIENCIA E TECNOLOGIA DE ALIMENTOS::TECNOLOGIA DE ALIMENTOS |
description |
he accessory gene regulator (agr) is a S. aureus global regulator of virulence factors, as the staphylococcal enterotoxins (SE), responsible for the staphylococcal food poisoning. There are four different agr groups due to the polymorphism in the amino acids sequence of the agrC and agrD. In the literature is described a relationship among agr groups and virulence factors, diseases, preferential host and antibiotic resistance. In this context, it was aimed to characterize the agr groups through biplex PCR, and relationship among agr groups, enterotoxigenic and antimicrobian profiles of S. aureus isolated from foods and bovine mastitis milk. A total of 115 strains were used to characterize the agr groups , being 30 isolated from f bovine mastitis milk and 85 isolated from several sources of foods. To assess the relationship between agr groups and enterotoxin production were used 14/85 strains previously characterized for the presence of some enterotoxins (sea, seb, sec, sed e cluster egc). To determine the profile of antibiotics resistance were used 71/115 strains. We observed a prevalence of agr group II with19.1% (22/115 strains), followed by the agr I with 8.6% (10/115 strains), agr III with 7.8% (9 / 115 strains), and agr IV with6.0% (7 / 115 strains). Among the strains isolated from bovine mastitis milk agr group I was prevailed with 20% (6/30 strains), whereas in the strains isolated from several food sources was observed prevalence of agr group II with 32.7% (18/85 strains), especially among those from chicken meat. Among the 14 strains (14/85) that contained enterotoxin genes, the majority of them contained the cluster egc (70%) belonged to agr II, whereas no relationship was found with those who had the genes for the classical SE (sea, seb, sec, sed). Considering the antibiotic resistance 100% of bovine mastitis milk strains and from various sources of food were resistant to penicillin, ampicillin, cefoxitin, and vancomycin. Relationship was observed between food strains, which were resistant to vancomycin and agr II, however, no relationship was found between antibiotic profile and agr groups among the strains isolated from bovine mastitis milk. These results demonstrated the prevalence of agr II among food strains and agr I among bovine mastitis milk strains. Moreover, the strains that carried the cluster egc were predominant agr II, which could indicate the occurrence of a clonal group among those. Another important result obtained in this study was the high rate of S. aureus multiresistant strains isolated from food, which emphasizes the importance of dissemination of these strains among foods. |
publishDate |
2009 |
dc.date.issued.fl_str_mv |
2009-10-27 |
dc.date.available.fl_str_mv |
2011-05-18 2014-08-20T13:42:05Z |
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2014-08-20T13:42:05Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/masterThesis |
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dc.identifier.citation.fl_str_mv |
BASSANI, Milena Tomasi. Caracterização de grupos agr e sua relação com perfil enterotoxigênico e antimicrobiano em Staphylococcus aureus isolados de diferentes origens.. 2009. 71 f. Dissertação (Mestrado em Agronomia) - Universidade Federal de Pelotas, Pelotas, 2009. |
dc.identifier.uri.fl_str_mv |
http://repositorio.ufpel.edu.br/handle/ri/1312 |
identifier_str_mv |
BASSANI, Milena Tomasi. Caracterização de grupos agr e sua relação com perfil enterotoxigênico e antimicrobiano em Staphylococcus aureus isolados de diferentes origens.. 2009. 71 f. Dissertação (Mestrado em Agronomia) - Universidade Federal de Pelotas, Pelotas, 2009. |
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UFPel |
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BR |
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Faculdade de Agronomia Eliseu Maciel |
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Universidade Federal de Pelotas |
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