Identification of a murine cell line that distinguishes virulent from attenuated isolates of the morbillivirus Peste des Petits Ruminants, a promising tool for virulence studies

Detalhes bibliográficos
Autor(a) principal: Comerlato, Juliana
Data de Publicação: 2020
Outros Autores: Albina, Emmanuel, Puech, Carinne, Franco, Ana Claudia, Minet, Cecile, Eloiflin, Roger Junior, Rodrigues, Valérie, Almeida, Renata Servan de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFRGS
Texto Completo: http://hdl.handle.net/10183/218444
Resumo: Comprehensive pathogenesis studies on Peste des Petits Ruminants virus (PPRV) have been delayed so far by the absence of a small animal model reproducing the disease or an in vitro biological system revealing virulence differences. In this study, a mouse 10T1/2 cell line has been identified as presenting different susceptibility to virulent and attenuated PPRV strains. As evidenced by immunofluorescence test and RT-PCR, both virulent and attenuated PPR viruses penetrated and initiated the replication cycle in 10T1/2 cells, independently of the presence of the SLAM goat receptor. However, only virulent strains successfully completed their replication cycle while the vaccine strains did not. Since 10T1/2 cells are interferon-producing cells, the role of the type I interferon (type I IFN) response on this differentiated replication between virulent and attenuated strains was verified by stimulation or repression. Modulation of the type I IFN response did not improve the replication of the vaccine strains, indicating that other cell factor(s) not yet established may hinder the replication of attenuated PPRV in 10T1/2. This 10T1/2 cell line can be proposed as a new in vitro tool for PPRV-host interaction and virulence studies.
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spelling Comerlato, JulianaAlbina, EmmanuelPuech, CarinneFranco, Ana ClaudiaMinet, CecileEloiflin, Roger JuniorRodrigues, ValérieAlmeida, Renata Servan de2021-03-05T03:59:11Z20200168-1702http://hdl.handle.net/10183/218444001122145Comprehensive pathogenesis studies on Peste des Petits Ruminants virus (PPRV) have been delayed so far by the absence of a small animal model reproducing the disease or an in vitro biological system revealing virulence differences. In this study, a mouse 10T1/2 cell line has been identified as presenting different susceptibility to virulent and attenuated PPRV strains. As evidenced by immunofluorescence test and RT-PCR, both virulent and attenuated PPR viruses penetrated and initiated the replication cycle in 10T1/2 cells, independently of the presence of the SLAM goat receptor. However, only virulent strains successfully completed their replication cycle while the vaccine strains did not. Since 10T1/2 cells are interferon-producing cells, the role of the type I interferon (type I IFN) response on this differentiated replication between virulent and attenuated strains was verified by stimulation or repression. Modulation of the type I IFN response did not improve the replication of the vaccine strains, indicating that other cell factor(s) not yet established may hinder the replication of attenuated PPRV in 10T1/2. This 10T1/2 cell line can be proposed as a new in vitro tool for PPRV-host interaction and virulence studies.application/pdfengVirus research. Amsterdam. Vol. 286 (Sept. 2020), 198035, 8 p.Peste dos pequenos ruminantesVírus da peste dos pequenos ruminantesMembro 1 da família de moléculas de sinalização da ativação linfocitáriaVirulênciaPPRV10T1/2 cellsCell permissivenessSLAM receptorType I IFNVirus-host interactionsIdentification of a murine cell line that distinguishes virulent from attenuated isolates of the morbillivirus Peste des Petits Ruminants, a promising tool for virulence studiesEstrangeiroinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSTEXT001122145.pdf.txt001122145.pdf.txtExtracted Texttext/plain48051http://www.lume.ufrgs.br/bitstream/10183/218444/2/001122145.pdf.txt8c1962a6d57e3b631becbadc23ea9685MD52ORIGINAL001122145.pdfTexto completo (inglês)application/pdf1274403http://www.lume.ufrgs.br/bitstream/10183/218444/1/001122145.pdf423bc4d04d03da0779f15cfee43f1c97MD5110183/2184442021-05-07 05:13:47.073859oai:www.lume.ufrgs.br:10183/218444Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2021-05-07T08:13:47Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false
dc.title.pt_BR.fl_str_mv Identification of a murine cell line that distinguishes virulent from attenuated isolates of the morbillivirus Peste des Petits Ruminants, a promising tool for virulence studies
title Identification of a murine cell line that distinguishes virulent from attenuated isolates of the morbillivirus Peste des Petits Ruminants, a promising tool for virulence studies
spellingShingle Identification of a murine cell line that distinguishes virulent from attenuated isolates of the morbillivirus Peste des Petits Ruminants, a promising tool for virulence studies
Comerlato, Juliana
Peste dos pequenos ruminantes
Vírus da peste dos pequenos ruminantes
Membro 1 da família de moléculas de sinalização da ativação linfocitária
Virulência
PPRV
10T1/2 cells
Cell permissiveness
SLAM receptor
Type I IFN
Virus-host interactions
title_short Identification of a murine cell line that distinguishes virulent from attenuated isolates of the morbillivirus Peste des Petits Ruminants, a promising tool for virulence studies
title_full Identification of a murine cell line that distinguishes virulent from attenuated isolates of the morbillivirus Peste des Petits Ruminants, a promising tool for virulence studies
title_fullStr Identification of a murine cell line that distinguishes virulent from attenuated isolates of the morbillivirus Peste des Petits Ruminants, a promising tool for virulence studies
title_full_unstemmed Identification of a murine cell line that distinguishes virulent from attenuated isolates of the morbillivirus Peste des Petits Ruminants, a promising tool for virulence studies
title_sort Identification of a murine cell line that distinguishes virulent from attenuated isolates of the morbillivirus Peste des Petits Ruminants, a promising tool for virulence studies
author Comerlato, Juliana
author_facet Comerlato, Juliana
Albina, Emmanuel
Puech, Carinne
Franco, Ana Claudia
Minet, Cecile
Eloiflin, Roger Junior
Rodrigues, Valérie
Almeida, Renata Servan de
author_role author
author2 Albina, Emmanuel
Puech, Carinne
Franco, Ana Claudia
Minet, Cecile
Eloiflin, Roger Junior
Rodrigues, Valérie
Almeida, Renata Servan de
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Comerlato, Juliana
Albina, Emmanuel
Puech, Carinne
Franco, Ana Claudia
Minet, Cecile
Eloiflin, Roger Junior
Rodrigues, Valérie
Almeida, Renata Servan de
dc.subject.por.fl_str_mv Peste dos pequenos ruminantes
Vírus da peste dos pequenos ruminantes
Membro 1 da família de moléculas de sinalização da ativação linfocitária
Virulência
topic Peste dos pequenos ruminantes
Vírus da peste dos pequenos ruminantes
Membro 1 da família de moléculas de sinalização da ativação linfocitária
Virulência
PPRV
10T1/2 cells
Cell permissiveness
SLAM receptor
Type I IFN
Virus-host interactions
dc.subject.eng.fl_str_mv PPRV
10T1/2 cells
Cell permissiveness
SLAM receptor
Type I IFN
Virus-host interactions
description Comprehensive pathogenesis studies on Peste des Petits Ruminants virus (PPRV) have been delayed so far by the absence of a small animal model reproducing the disease or an in vitro biological system revealing virulence differences. In this study, a mouse 10T1/2 cell line has been identified as presenting different susceptibility to virulent and attenuated PPRV strains. As evidenced by immunofluorescence test and RT-PCR, both virulent and attenuated PPR viruses penetrated and initiated the replication cycle in 10T1/2 cells, independently of the presence of the SLAM goat receptor. However, only virulent strains successfully completed their replication cycle while the vaccine strains did not. Since 10T1/2 cells are interferon-producing cells, the role of the type I interferon (type I IFN) response on this differentiated replication between virulent and attenuated strains was verified by stimulation or repression. Modulation of the type I IFN response did not improve the replication of the vaccine strains, indicating that other cell factor(s) not yet established may hinder the replication of attenuated PPRV in 10T1/2. This 10T1/2 cell line can be proposed as a new in vitro tool for PPRV-host interaction and virulence studies.
publishDate 2020
dc.date.issued.fl_str_mv 2020
dc.date.accessioned.fl_str_mv 2021-03-05T03:59:11Z
dc.type.driver.fl_str_mv Estrangeiro
info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
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status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10183/218444
dc.identifier.issn.pt_BR.fl_str_mv 0168-1702
dc.identifier.nrb.pt_BR.fl_str_mv 001122145
identifier_str_mv 0168-1702
001122145
url http://hdl.handle.net/10183/218444
dc.language.iso.fl_str_mv eng
language eng
dc.relation.ispartof.pt_BR.fl_str_mv Virus research. Amsterdam. Vol. 286 (Sept. 2020), 198035, 8 p.
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFRGS
instname:Universidade Federal do Rio Grande do Sul (UFRGS)
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instname_str Universidade Federal do Rio Grande do Sul (UFRGS)
instacron_str UFRGS
institution UFRGS
reponame_str Repositório Institucional da UFRGS
collection Repositório Institucional da UFRGS
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