Avaliação de heterorresistência ao imipenem em enterobactérias produtoras e não-produtoras de kpc
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Tipo de documento: | Trabalho de conclusão de curso |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFRGS |
Texto Completo: | http://hdl.handle.net/10183/144032 |
Resumo: | The misdetection of Klebsiella pneumoniae carbapenemase (KPC) may lead to treatment failure and favour the spread of this resistance mechanism. Heteroresistance is an antimicrobial resistance in a subset of an isolate considered to be susceptible by conventional testing. The aim of this study was to evaluate the presence of heteroresistance to imipenem in KPC-producing and non-producing K. pneumoniae and E. coli. After the determination of the minimum inhibitory concentration (MIC) to imipenem by broth microdilution, each isolate with MIC lower than 4 mg L-1 was subcultured in plates containing imipenem ranging from 0.25 mg L-1 to 32 mg L-1. Isolates with growth in imipenem concentrations two fold dilutions above the original MIC were considered heteroresistant. The KPC group consisted of 10 isolates, while the non-KPC group consisted of four isolates and distinct results were observed among them: all KPC-producing isolates presented heteroresistance to imipenem, while for the KPC-non-producing isolates this phenomenon was not observed. Interestingly, we also found heteroresistance in E. coli isolates, which, to best of our knowledge, was not reported as yet. Our results indicated a possible relationship between heteroresistance and KPC production. The clinical implications of appearing of heteroresistant subpopulations remains to be investigated, considering that this phenomenon seems to be frequent in Gram-negative rods, including members, such as E. coli, in which this phenomenon is still rarely investigated. |
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Nodari, Carolina SilvaBarth, Afonso LuisRibeiro, Vanessa Bley2016-07-28T02:16:06Z2013http://hdl.handle.net/10183/144032000914725The misdetection of Klebsiella pneumoniae carbapenemase (KPC) may lead to treatment failure and favour the spread of this resistance mechanism. Heteroresistance is an antimicrobial resistance in a subset of an isolate considered to be susceptible by conventional testing. The aim of this study was to evaluate the presence of heteroresistance to imipenem in KPC-producing and non-producing K. pneumoniae and E. coli. After the determination of the minimum inhibitory concentration (MIC) to imipenem by broth microdilution, each isolate with MIC lower than 4 mg L-1 was subcultured in plates containing imipenem ranging from 0.25 mg L-1 to 32 mg L-1. Isolates with growth in imipenem concentrations two fold dilutions above the original MIC were considered heteroresistant. The KPC group consisted of 10 isolates, while the non-KPC group consisted of four isolates and distinct results were observed among them: all KPC-producing isolates presented heteroresistance to imipenem, while for the KPC-non-producing isolates this phenomenon was not observed. Interestingly, we also found heteroresistance in E. coli isolates, which, to best of our knowledge, was not reported as yet. Our results indicated a possible relationship between heteroresistance and KPC production. The clinical implications of appearing of heteroresistant subpopulations remains to be investigated, considering that this phenomenon seems to be frequent in Gram-negative rods, including members, such as E. coli, in which this phenomenon is still rarely investigated.application/pdfporImipenemResistência microbiana a medicamentosKlebsiella pneumoniaeKPCE. coliImipenemHeteroresistanceAvaliação de heterorresistência ao imipenem em enterobactérias produtoras e não-produtoras de kpcImipenem heteroresistance: evaluation of Klebsiella pneumoniae carbapenemase-producing and non-producing K. pneumoniae and E. coliinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/bachelorThesisUniversidade Federal do Rio Grande do SulFaculdade de FarmáciaPorto Alegre, BR-RS2013Farmáciagraduaçãoinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSORIGINAL000914725.pdf000914725.pdfTexto completoapplication/pdf527465http://www.lume.ufrgs.br/bitstream/10183/144032/1/000914725.pdfef5f2a07bb86e1fa6c22b046946a76d0MD51TEXT000914725.pdf.txt000914725.pdf.txtExtracted Texttext/plain13937http://www.lume.ufrgs.br/bitstream/10183/144032/2/000914725.pdf.txtce4eb3ae2e32b9208749cd3c26931f11MD52THUMBNAIL000914725.pdf.jpg000914725.pdf.jpgGenerated Thumbnailimage/jpeg1085http://www.lume.ufrgs.br/bitstream/10183/144032/3/000914725.pdf.jpgf4d0cfc7966de04662d8677a7b98218cMD5310183/1440322018-10-29 07:43:37.504oai:www.lume.ufrgs.br:10183/144032Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2018-10-29T10:43:37Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
Avaliação de heterorresistência ao imipenem em enterobactérias produtoras e não-produtoras de kpc |
dc.title.alternative.en.fl_str_mv |
Imipenem heteroresistance: evaluation of Klebsiella pneumoniae carbapenemase-producing and non-producing K. pneumoniae and E. coli |
title |
Avaliação de heterorresistência ao imipenem em enterobactérias produtoras e não-produtoras de kpc |
spellingShingle |
Avaliação de heterorresistência ao imipenem em enterobactérias produtoras e não-produtoras de kpc Nodari, Carolina Silva Imipenem Resistência microbiana a medicamentos Klebsiella pneumoniae KPC E. coli Imipenem Heteroresistance |
title_short |
Avaliação de heterorresistência ao imipenem em enterobactérias produtoras e não-produtoras de kpc |
title_full |
Avaliação de heterorresistência ao imipenem em enterobactérias produtoras e não-produtoras de kpc |
title_fullStr |
Avaliação de heterorresistência ao imipenem em enterobactérias produtoras e não-produtoras de kpc |
title_full_unstemmed |
Avaliação de heterorresistência ao imipenem em enterobactérias produtoras e não-produtoras de kpc |
title_sort |
Avaliação de heterorresistência ao imipenem em enterobactérias produtoras e não-produtoras de kpc |
author |
Nodari, Carolina Silva |
author_facet |
Nodari, Carolina Silva |
author_role |
author |
dc.contributor.author.fl_str_mv |
Nodari, Carolina Silva |
dc.contributor.advisor1.fl_str_mv |
Barth, Afonso Luis |
dc.contributor.advisor-co1.fl_str_mv |
Ribeiro, Vanessa Bley |
contributor_str_mv |
Barth, Afonso Luis Ribeiro, Vanessa Bley |
dc.subject.por.fl_str_mv |
Imipenem Resistência microbiana a medicamentos Klebsiella pneumoniae |
topic |
Imipenem Resistência microbiana a medicamentos Klebsiella pneumoniae KPC E. coli Imipenem Heteroresistance |
dc.subject.eng.fl_str_mv |
KPC E. coli Imipenem Heteroresistance |
description |
The misdetection of Klebsiella pneumoniae carbapenemase (KPC) may lead to treatment failure and favour the spread of this resistance mechanism. Heteroresistance is an antimicrobial resistance in a subset of an isolate considered to be susceptible by conventional testing. The aim of this study was to evaluate the presence of heteroresistance to imipenem in KPC-producing and non-producing K. pneumoniae and E. coli. After the determination of the minimum inhibitory concentration (MIC) to imipenem by broth microdilution, each isolate with MIC lower than 4 mg L-1 was subcultured in plates containing imipenem ranging from 0.25 mg L-1 to 32 mg L-1. Isolates with growth in imipenem concentrations two fold dilutions above the original MIC were considered heteroresistant. The KPC group consisted of 10 isolates, while the non-KPC group consisted of four isolates and distinct results were observed among them: all KPC-producing isolates presented heteroresistance to imipenem, while for the KPC-non-producing isolates this phenomenon was not observed. Interestingly, we also found heteroresistance in E. coli isolates, which, to best of our knowledge, was not reported as yet. Our results indicated a possible relationship between heteroresistance and KPC production. The clinical implications of appearing of heteroresistant subpopulations remains to be investigated, considering that this phenomenon seems to be frequent in Gram-negative rods, including members, such as E. coli, in which this phenomenon is still rarely investigated. |
publishDate |
2013 |
dc.date.issued.fl_str_mv |
2013 |
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2016-07-28T02:16:06Z |
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