Detection of virulence genes in Salmonella Heidelberg isolated from chicken carcasses
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRGS |
Texto Completo: | http://hdl.handle.net/10183/201852 |
Resumo: | During the last years, Brazilian government control programs have detected an increase of Salmonella Heidelberg in poultry slaughterhouses a condition that poses a threat to human health However, the reasons remain unclear. Differences in genetic virulence profiles may be a possible justification. In addition, effective control of Salmonella is related to an efficient epidemiological surveillance system through genotyping techniques. In this context, the aim of this study was the detection of 24 virulence-associated genes in 126 S. Heidelberg isolates. We classified the isolates into 56 different genetic profiles. None of the isolates presented all the virulence genes. The prevalence of these genes was high in all tested samples as the lowest number of genes detected in one isolate was 10/24. The lpfA and csgA (fimbriae), invA and sivH (TTSS), and msgA and tolC (intracellular survival) genes were present in 100% of the isolates analyzed. Genes encoding effector proteins were detected in the majority of SH isolates. No single isolate had the sefA gene. The pefA gene was found in only four isolates. We have also performed a screening of genes associated with iron metabolism: 88.9% of isolates had the iroN gene and 79.4% the sitC gene. Although all the isolates belong to the same serotype, several genotypic profiles were observed. These findings suggest that there is a diversity of S. Heidelberg isolates in poultry products. The fact that a single predominant profile was not found in this study indicates the presence of variable sources of contamination caused by SH. The detection of genetic profiles of Salmonella strains can be used to determine the virulence patterns of SH isolates. |
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Webber, BrunaBorges, Karen ApellanisFurian, Thales QuediRizzo, Natalie N.Tondo, Eduardo CesarSantos, Luciana Ruschel dosRodrigues, Laura BeatrizNascimento, Vladimir Pinheiro do2019-11-15T03:52:24Z20190036-4665http://hdl.handle.net/10183/201852001104547During the last years, Brazilian government control programs have detected an increase of Salmonella Heidelberg in poultry slaughterhouses a condition that poses a threat to human health However, the reasons remain unclear. Differences in genetic virulence profiles may be a possible justification. In addition, effective control of Salmonella is related to an efficient epidemiological surveillance system through genotyping techniques. In this context, the aim of this study was the detection of 24 virulence-associated genes in 126 S. Heidelberg isolates. We classified the isolates into 56 different genetic profiles. None of the isolates presented all the virulence genes. The prevalence of these genes was high in all tested samples as the lowest number of genes detected in one isolate was 10/24. The lpfA and csgA (fimbriae), invA and sivH (TTSS), and msgA and tolC (intracellular survival) genes were present in 100% of the isolates analyzed. Genes encoding effector proteins were detected in the majority of SH isolates. No single isolate had the sefA gene. The pefA gene was found in only four isolates. We have also performed a screening of genes associated with iron metabolism: 88.9% of isolates had the iroN gene and 79.4% the sitC gene. Although all the isolates belong to the same serotype, several genotypic profiles were observed. These findings suggest that there is a diversity of S. Heidelberg isolates in poultry products. The fact that a single predominant profile was not found in this study indicates the presence of variable sources of contamination caused by SH. The detection of genetic profiles of Salmonella strains can be used to determine the virulence patterns of SH isolates.application/pdfengRevista do Instituto de Medicina Tropical de São Paulo = Journal of the São Paulo Institute of Tropical Medicine. São Paulo. Vol. 61 (2019), e36, [7] p.Salmonella HeidelbergAves domesticasFatores de virulênciaVariação genéticaReação em cadeia da polimeraseSalmonellosisSalmonella HeidelbergPoultryPCRVirulence genesDetection of virulence genes in Salmonella Heidelberg isolated from chicken carcassesinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/otherinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSTEXT001104547.pdf.txt001104547.pdf.txtExtracted Texttext/plain30763http://www.lume.ufrgs.br/bitstream/10183/201852/2/001104547.pdf.txtc775792178768ace8e054777d7fdfdfcMD52ORIGINAL001104547.pdfTexto completo (inglês)application/pdf187019http://www.lume.ufrgs.br/bitstream/10183/201852/1/001104547.pdfd5c9da8fee84642173989988e87c1067MD5110183/2018522023-09-27 03:36:50.648601oai:www.lume.ufrgs.br:10183/201852Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2023-09-27T06:36:50Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
Detection of virulence genes in Salmonella Heidelberg isolated from chicken carcasses |
title |
Detection of virulence genes in Salmonella Heidelberg isolated from chicken carcasses |
spellingShingle |
Detection of virulence genes in Salmonella Heidelberg isolated from chicken carcasses Webber, Bruna Salmonella Heidelberg Aves domesticas Fatores de virulência Variação genética Reação em cadeia da polimerase Salmonellosis Salmonella Heidelberg Poultry PCR Virulence genes |
title_short |
Detection of virulence genes in Salmonella Heidelberg isolated from chicken carcasses |
title_full |
Detection of virulence genes in Salmonella Heidelberg isolated from chicken carcasses |
title_fullStr |
Detection of virulence genes in Salmonella Heidelberg isolated from chicken carcasses |
title_full_unstemmed |
Detection of virulence genes in Salmonella Heidelberg isolated from chicken carcasses |
title_sort |
Detection of virulence genes in Salmonella Heidelberg isolated from chicken carcasses |
author |
Webber, Bruna |
author_facet |
Webber, Bruna Borges, Karen Apellanis Furian, Thales Quedi Rizzo, Natalie N. Tondo, Eduardo Cesar Santos, Luciana Ruschel dos Rodrigues, Laura Beatriz Nascimento, Vladimir Pinheiro do |
author_role |
author |
author2 |
Borges, Karen Apellanis Furian, Thales Quedi Rizzo, Natalie N. Tondo, Eduardo Cesar Santos, Luciana Ruschel dos Rodrigues, Laura Beatriz Nascimento, Vladimir Pinheiro do |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Webber, Bruna Borges, Karen Apellanis Furian, Thales Quedi Rizzo, Natalie N. Tondo, Eduardo Cesar Santos, Luciana Ruschel dos Rodrigues, Laura Beatriz Nascimento, Vladimir Pinheiro do |
dc.subject.por.fl_str_mv |
Salmonella Heidelberg Aves domesticas Fatores de virulência Variação genética Reação em cadeia da polimerase |
topic |
Salmonella Heidelberg Aves domesticas Fatores de virulência Variação genética Reação em cadeia da polimerase Salmonellosis Salmonella Heidelberg Poultry PCR Virulence genes |
dc.subject.eng.fl_str_mv |
Salmonellosis Salmonella Heidelberg Poultry PCR Virulence genes |
description |
During the last years, Brazilian government control programs have detected an increase of Salmonella Heidelberg in poultry slaughterhouses a condition that poses a threat to human health However, the reasons remain unclear. Differences in genetic virulence profiles may be a possible justification. In addition, effective control of Salmonella is related to an efficient epidemiological surveillance system through genotyping techniques. In this context, the aim of this study was the detection of 24 virulence-associated genes in 126 S. Heidelberg isolates. We classified the isolates into 56 different genetic profiles. None of the isolates presented all the virulence genes. The prevalence of these genes was high in all tested samples as the lowest number of genes detected in one isolate was 10/24. The lpfA and csgA (fimbriae), invA and sivH (TTSS), and msgA and tolC (intracellular survival) genes were present in 100% of the isolates analyzed. Genes encoding effector proteins were detected in the majority of SH isolates. No single isolate had the sefA gene. The pefA gene was found in only four isolates. We have also performed a screening of genes associated with iron metabolism: 88.9% of isolates had the iroN gene and 79.4% the sitC gene. Although all the isolates belong to the same serotype, several genotypic profiles were observed. These findings suggest that there is a diversity of S. Heidelberg isolates in poultry products. The fact that a single predominant profile was not found in this study indicates the presence of variable sources of contamination caused by SH. The detection of genetic profiles of Salmonella strains can be used to determine the virulence patterns of SH isolates. |
publishDate |
2019 |
dc.date.accessioned.fl_str_mv |
2019-11-15T03:52:24Z |
dc.date.issued.fl_str_mv |
2019 |
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http://hdl.handle.net/10183/201852 |
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0036-4665 |
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001104547 |
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http://hdl.handle.net/10183/201852 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.pt_BR.fl_str_mv |
Revista do Instituto de Medicina Tropical de São Paulo = Journal of the São Paulo Institute of Tropical Medicine. São Paulo. Vol. 61 (2019), e36, [7] p. |
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info:eu-repo/semantics/openAccess |
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