Pharmacological action of tick saliva upon haemostasis and the neutralization ability of sera from repeatedly infested hosts
Autor(a) principal: | |
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Data de Publicação: | 2009 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRGS |
Texto Completo: | http://hdl.handle.net/10183/27465 |
Resumo: | Ticks are blood-feeding arthropods widely distributed in the world and vectors of several diseases. As haematophagy demands evasion strategies and repeatedly infested hosts develop protective immune responses, we investigated the mechanisms of the Rhipicephalus (Boophilus) microplus saliva anti-haemostatic activity and the possible relationship between the acquired natural anti-tick host resistance and anti-haemostatic action. For this purpose, we studied the effects of R. microplus saliva on different pathways of haemostasis and tested whether repeated infested bovine sera (RIBS) are able to abolish salivary anti-haemostatic activities. R. microplus saliva (i) displays inhibitory activity upon collagen-induced platelet aggregation; (ii) inhibits the induction of endothelial pro-coagulant state ; and (iii) reduces thrombogenesis in vivo. RIBS were shown to be able to partially block the delay of coagulation and the anti-thrombotic effect of saliva, and to totally abolish the modulation of endothelium activation. Conversely, RIBS has no effect on the inhibition of platelet aggregation. These results show, for the first time, the neutralization ability of sera from acquired resistance hosts against tick antihaemostatics. Moreover, this is the first report of a haematophagous parasite able to modulate endothelial cell procoagulant state, and addresses the presence of anti-platelet and anti-thrombotic activity in R. microplus saliva. |
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Reck Junior, JoséOliveira, Markus BergerMarks, Fernanda SimoneZingali, Russolina BenedetaCanal, Cláudio WageckFerreira, Carlos Alexandre SanchezGuimaraes, Jorge AlmeidaTermignoni, Carlos2011-01-21T05:59:03Z20090031-1820http://hdl.handle.net/10183/27465000750325Ticks are blood-feeding arthropods widely distributed in the world and vectors of several diseases. As haematophagy demands evasion strategies and repeatedly infested hosts develop protective immune responses, we investigated the mechanisms of the Rhipicephalus (Boophilus) microplus saliva anti-haemostatic activity and the possible relationship between the acquired natural anti-tick host resistance and anti-haemostatic action. For this purpose, we studied the effects of R. microplus saliva on different pathways of haemostasis and tested whether repeated infested bovine sera (RIBS) are able to abolish salivary anti-haemostatic activities. R. microplus saliva (i) displays inhibitory activity upon collagen-induced platelet aggregation; (ii) inhibits the induction of endothelial pro-coagulant state ; and (iii) reduces thrombogenesis in vivo. RIBS were shown to be able to partially block the delay of coagulation and the anti-thrombotic effect of saliva, and to totally abolish the modulation of endothelium activation. Conversely, RIBS has no effect on the inhibition of platelet aggregation. These results show, for the first time, the neutralization ability of sera from acquired resistance hosts against tick antihaemostatics. Moreover, this is the first report of a haematophagous parasite able to modulate endothelial cell procoagulant state, and addresses the presence of anti-platelet and anti-thrombotic activity in R. microplus saliva.application/pdfengParasitology. Cambridge, United Kingdom. Vol. 136, no. 11 (Sept. 2009), p. 1339-1349Boophilus microplusCarrapatosHemostasiaCoagulação sanguíneaBioquímicaRhipicephalus (Boophilus) microplusTickHaematophagousHaemostasisCoagulationPlateletEndotheliumThrombosisBovine resistancePharmacological action of tick saliva upon haemostasis and the neutralization ability of sera from repeatedly infested hostsEstrangeiroinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSORIGINAL000750325.pdf000750325.pdfTexto completo (inglês)application/pdf176510http://www.lume.ufrgs.br/bitstream/10183/27465/1/000750325.pdf435c7e23c1e43c34c7d12e2c80b59df0MD51TEXT000750325.pdf.txt000750325.pdf.txtExtracted Texttext/plain53847http://www.lume.ufrgs.br/bitstream/10183/27465/2/000750325.pdf.txt237ce8d967ff4d3ea93739b8183237b6MD52THUMBNAIL000750325.pdf.jpg000750325.pdf.jpgGenerated Thumbnailimage/jpeg1933http://www.lume.ufrgs.br/bitstream/10183/27465/3/000750325.pdf.jpg62dd0f5e9460d1917e1d09daf0724731MD5310183/274652021-06-26 04:46:42.049278oai:www.lume.ufrgs.br:10183/27465Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2021-06-26T07:46:42Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
Pharmacological action of tick saliva upon haemostasis and the neutralization ability of sera from repeatedly infested hosts |
title |
Pharmacological action of tick saliva upon haemostasis and the neutralization ability of sera from repeatedly infested hosts |
spellingShingle |
Pharmacological action of tick saliva upon haemostasis and the neutralization ability of sera from repeatedly infested hosts Reck Junior, José Boophilus microplus Carrapatos Hemostasia Coagulação sanguínea Bioquímica Rhipicephalus (Boophilus) microplus Tick Haematophagous Haemostasis Coagulation Platelet Endothelium Thrombosis Bovine resistance |
title_short |
Pharmacological action of tick saliva upon haemostasis and the neutralization ability of sera from repeatedly infested hosts |
title_full |
Pharmacological action of tick saliva upon haemostasis and the neutralization ability of sera from repeatedly infested hosts |
title_fullStr |
Pharmacological action of tick saliva upon haemostasis and the neutralization ability of sera from repeatedly infested hosts |
title_full_unstemmed |
Pharmacological action of tick saliva upon haemostasis and the neutralization ability of sera from repeatedly infested hosts |
title_sort |
Pharmacological action of tick saliva upon haemostasis and the neutralization ability of sera from repeatedly infested hosts |
author |
Reck Junior, José |
author_facet |
Reck Junior, José Oliveira, Markus Berger Marks, Fernanda Simone Zingali, Russolina Benedeta Canal, Cláudio Wageck Ferreira, Carlos Alexandre Sanchez Guimaraes, Jorge Almeida Termignoni, Carlos |
author_role |
author |
author2 |
Oliveira, Markus Berger Marks, Fernanda Simone Zingali, Russolina Benedeta Canal, Cláudio Wageck Ferreira, Carlos Alexandre Sanchez Guimaraes, Jorge Almeida Termignoni, Carlos |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Reck Junior, José Oliveira, Markus Berger Marks, Fernanda Simone Zingali, Russolina Benedeta Canal, Cláudio Wageck Ferreira, Carlos Alexandre Sanchez Guimaraes, Jorge Almeida Termignoni, Carlos |
dc.subject.por.fl_str_mv |
Boophilus microplus Carrapatos Hemostasia Coagulação sanguínea Bioquímica |
topic |
Boophilus microplus Carrapatos Hemostasia Coagulação sanguínea Bioquímica Rhipicephalus (Boophilus) microplus Tick Haematophagous Haemostasis Coagulation Platelet Endothelium Thrombosis Bovine resistance |
dc.subject.eng.fl_str_mv |
Rhipicephalus (Boophilus) microplus Tick Haematophagous Haemostasis Coagulation Platelet Endothelium Thrombosis Bovine resistance |
description |
Ticks are blood-feeding arthropods widely distributed in the world and vectors of several diseases. As haematophagy demands evasion strategies and repeatedly infested hosts develop protective immune responses, we investigated the mechanisms of the Rhipicephalus (Boophilus) microplus saliva anti-haemostatic activity and the possible relationship between the acquired natural anti-tick host resistance and anti-haemostatic action. For this purpose, we studied the effects of R. microplus saliva on different pathways of haemostasis and tested whether repeated infested bovine sera (RIBS) are able to abolish salivary anti-haemostatic activities. R. microplus saliva (i) displays inhibitory activity upon collagen-induced platelet aggregation; (ii) inhibits the induction of endothelial pro-coagulant state ; and (iii) reduces thrombogenesis in vivo. RIBS were shown to be able to partially block the delay of coagulation and the anti-thrombotic effect of saliva, and to totally abolish the modulation of endothelium activation. Conversely, RIBS has no effect on the inhibition of platelet aggregation. These results show, for the first time, the neutralization ability of sera from acquired resistance hosts against tick antihaemostatics. Moreover, this is the first report of a haematophagous parasite able to modulate endothelial cell procoagulant state, and addresses the presence of anti-platelet and anti-thrombotic activity in R. microplus saliva. |
publishDate |
2009 |
dc.date.issued.fl_str_mv |
2009 |
dc.date.accessioned.fl_str_mv |
2011-01-21T05:59:03Z |
dc.type.driver.fl_str_mv |
Estrangeiro info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10183/27465 |
dc.identifier.issn.pt_BR.fl_str_mv |
0031-1820 |
dc.identifier.nrb.pt_BR.fl_str_mv |
000750325 |
identifier_str_mv |
0031-1820 000750325 |
url |
http://hdl.handle.net/10183/27465 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.pt_BR.fl_str_mv |
Parasitology. Cambridge, United Kingdom. Vol. 136, no. 11 (Sept. 2009), p. 1339-1349 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
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