Estudo qu?mico e atividades biol?gicas de Ouratea hexasperma var. planchonii Engl. (Ochnaceae)
Autor(a) principal: | |
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Data de Publicação: | 2008 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Biblioteca Digital de Teses e Dissertações da UFRRJ |
Texto Completo: | https://tede.ufrrj.br/jspui/handle/jspui/3842 |
Resumo: | The specials metabolites isolated from the branches of the Ouratea hexasperma var. planchonii Engl., allowing to the comparison with the chemical constituents from variety Ouratea hexasperma (St. Hil.) to confirm the botanical classification. Some biological activities were evaluated. The analysis of the fractions from solvents and chromatographic fractionation of the dichloromethane and methanolic extracts from the branches of Ouratea hexasperma var. planchonii Engl. led to the identification of some chemical constituents. This procedure led to identification of aliphatics compounds mixture, aliphatic alcohol mixture, steroids mixture (sitosterol, stigmasterol and campesterol), three triterpenes, lupeol e -amiryn e -amiryn, and the biflavonoids (7?-O-methylagathisflavone and agathisflavone) and sitosterol-3-O- -D-glycopyranoside. The structures were determined by IR, de 1H (1D e 2D) e 13C (BBD e DEPT) NMR spectral data analysis and comparison with literature values. The HPLC-MS were used in the analysis of two fractions containing the these two biflavones and some additional compounds. This analysis led us to identify the presence of methyl-vitexin, orientin, methyl-dihydroxyflavone, glycopiranosil-flavones, dimethylagathisflavone and glycopyranosil-prenyl-dihydroxyflavonol besides the confirmation of these two biflavonoids. The extracts from the branches of Ouratea hexasperma var. planchonii Engl. were submitted on evaluations of the antibacterial, antifungal, insecticide and toxicity activities. The Escherichia coli and Staphylococcus aureus growth was inhibited with methanolic extract. The fractions containing the biflavonoids were partially active as insecticide against Musca domestica. The extracts presented low toxicity on unique dose. |
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Carvalho, Mario Geraldo deCarvalho, Mario Geraldo deLima, Marco Edilson Freire deWerle, Alceni AugustaCarvalho, Geizi Jane Alves dehttp://lattes.cnpq.br/2500280502687742Fernandes, Renata Duarte2020-08-24T21:14:28Z2008-07-11FERNANDES, Renata Duarte. Estudo qu?mico e atividades biol?gicas de Ouratea hexasperma var. planchonii Engl. (Ochnaceae). 2008. 101 f. Disserta??o (Mestrado em Qu?mica Org?nica) - Instituto de Ci?ncias Exatas, Departamento de Qu?mica, Universidade Federal Rural do Rio de Janeiro, Serop?dica - RJ, 2008.https://tede.ufrrj.br/jspui/handle/jspui/3842The specials metabolites isolated from the branches of the Ouratea hexasperma var. planchonii Engl., allowing to the comparison with the chemical constituents from variety Ouratea hexasperma (St. Hil.) to confirm the botanical classification. Some biological activities were evaluated. The analysis of the fractions from solvents and chromatographic fractionation of the dichloromethane and methanolic extracts from the branches of Ouratea hexasperma var. planchonii Engl. led to the identification of some chemical constituents. This procedure led to identification of aliphatics compounds mixture, aliphatic alcohol mixture, steroids mixture (sitosterol, stigmasterol and campesterol), three triterpenes, lupeol e -amiryn e -amiryn, and the biflavonoids (7?-O-methylagathisflavone and agathisflavone) and sitosterol-3-O- -D-glycopyranoside. The structures were determined by IR, de 1H (1D e 2D) e 13C (BBD e DEPT) NMR spectral data analysis and comparison with literature values. The HPLC-MS were used in the analysis of two fractions containing the these two biflavones and some additional compounds. This analysis led us to identify the presence of methyl-vitexin, orientin, methyl-dihydroxyflavone, glycopiranosil-flavones, dimethylagathisflavone and glycopyranosil-prenyl-dihydroxyflavonol besides the confirmation of these two biflavonoids. The extracts from the branches of Ouratea hexasperma var. planchonii Engl. were submitted on evaluations of the antibacterial, antifungal, insecticide and toxicity activities. The Escherichia coli and Staphylococcus aureus growth was inhibited with methanolic extract. The fractions containing the biflavonoids were partially active as insecticide against Musca domestica. The extracts presented low toxicity on unique dose.Os metab?litos especiais isolados de galhos de Ouratea hexasperma var. planchonii Engl., permitiram a compara??o com os constituintes de O. hexasperma (St. Hil.) para confirmar a classifica??o bot?nica da mesma. Neste trabalho tamb?m foram avaliadas algumas atividades biol?gicas. As analises das fra??es obtidas do fracionamento com parti??o com solventes e t?cnicas cromatogr?ficas dos extratos preparados com diclorometano e metanol dos galhos de Ouratea hexasperma var. planchonii Engl. conduziu ? identifica??o de alguns constituintes qu?micos. Identificaram-se misturas de hidrocarbonetos alif?ticos, ?cidos alilf?ticos, de ester?ides (sitosterol, estigmasterol e campesterol), al?m de tr?s triterpenos, lupeol, ? e ? amirina, e os flavon?ides (7?-O-metilagatisflavona e agatisflavona) e sitosterol-3-O- -D-glicopiranosideo. As estruturas foram determinadas atrav?s de an?lise dos dados espectrom?tricos de IV, RMN de 1H e 13C uni e bidimensional e massas, al?m de compara??o com dados da literatura. A t?cnica CLAE-EM foi utilizada na an?lise adicional de duas fra??es contendo dois biflavon?ides e alguns constituintes adicionais. Esta an?lise permitiu propor a presen?a de metil-vitexina, orientina, metil-diidroxiflavona, glicopiranosil-flavonas, dimetilagatisflavona, glicopiranosil-prenil-flavonol al?m da confirma??o das duas biflavonas identificadas inicialmente. Os extratos dos galhos de Ouratea hexasperma var. planchonii Engl. foram submetidos ? avalia??o de atividade antibacteriana, antif?ngica, inseticida e de toxidez. O crescimento de Escherichia coli e Staphylococcus aureus reduziu com extrato metan?lico. As fra??es contendo as biflavonas foram parcialmente ativas como inseticida contra Musca domestica. Os extratos apresentaram baixa toxicidade em dose ?nica.Submitted by Jorge Silva (jorgelmsilva@ufrrj.br) on 2020-08-24T21:14:27Z No. of bitstreams: 1 2008 - Renata Duarte Fernandes.pdf: 4154849 bytes, checksum: ee649c72cfc24aa6b768fad70f02c665 (MD5)Made available in DSpace on 2020-08-24T21:14:28Z (GMT). No. of bitstreams: 1 2008 - Renata Duarte Fernandes.pdf: 4154849 bytes, checksum: ee649c72cfc24aa6b768fad70f02c665 (MD5) Previous issue date: 2008-07-11Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico - CNPqapplication/pdfhttps://tede.ufrrj.br/retrieve/61728/2008%20-%20Renata%20Duarte%20Fernandes.pdf.jpgporUniversidade Federal Rural do Rio de JaneiroPrograma de P?s-Gradua??o em Qu?micaUFRRJBrasilInstituto de Ci?ncias ExatasOuratea hexaspermaflavon?idesatividades biol?gicasflavonoidsbiologicals activitiesQu?micaEstudo qu?mico e atividades biol?gicas de Ouratea hexasperma var. planchonii Engl. (Ochnaceae)Phytochemical study and biological activities extracts from the branches of Ouratea hexasperma var. planchonii Engl.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da UFRRJinstname:Universidade Federal Rural do Rio de Janeiro (UFRRJ)instacron:UFRRJTHUMBNAIL2008 - Renata Duarte Fernandes.pdf.jpg2008 - Renata Duarte Fernandes.pdf.jpgimage/jpeg4121http://localhost:8080/tede/bitstream/jspui/3842/4/2008+-+Renata+Duarte+Fernandes.pdf.jpgf5a0e37ba9223f7fd0be2840be7ebf06MD54TEXT2008 - Renata Duarte Fernandes.pdf.txt2008 - Renata Duarte Fernandes.pdf.txttext/plain294459http://localhost:8080/tede/bitstream/jspui/3842/3/2008+-+Renata+Duarte+Fernandes.pdf.txt6afd7da748c71455558616be90520419MD53ORIGINAL2008 - Renata Duarte Fernandes.pdf2008 - Renata Duarte Fernandes.pdfapplication/pdf4154849http://localhost:8080/tede/bitstream/jspui/3842/2/2008+-+Renata+Duarte+Fernandes.pdfee649c72cfc24aa6b768fad70f02c665MD52LICENSElicense.txtlicense.txttext/plain; charset=utf-82089http://localhost:8080/tede/bitstream/jspui/3842/1/license.txt7b5ba3d2445355f386edab96125d42b7MD51jspui/38422021-04-22 17:10:11.231oai:localhost: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Biblioteca Digital de Teses e Dissertaçõeshttps://tede.ufrrj.br/PUBhttps://tede.ufrrj.br/oai/requestbibliot@ufrrj.br||bibliot@ufrrj.bropendoar:2021-04-22T20:10:11Biblioteca Digital de Teses e Dissertações da UFRRJ - Universidade Federal Rural do Rio de Janeiro (UFRRJ)false |
dc.title.por.fl_str_mv |
Estudo qu?mico e atividades biol?gicas de Ouratea hexasperma var. planchonii Engl. (Ochnaceae) |
dc.title.alternative.eng.fl_str_mv |
Phytochemical study and biological activities extracts from the branches of Ouratea hexasperma var. planchonii Engl. |
title |
Estudo qu?mico e atividades biol?gicas de Ouratea hexasperma var. planchonii Engl. (Ochnaceae) |
spellingShingle |
Estudo qu?mico e atividades biol?gicas de Ouratea hexasperma var. planchonii Engl. (Ochnaceae) Fernandes, Renata Duarte Ouratea hexasperma flavon?ides atividades biol?gicas flavonoids biologicals activities Qu?mica |
title_short |
Estudo qu?mico e atividades biol?gicas de Ouratea hexasperma var. planchonii Engl. (Ochnaceae) |
title_full |
Estudo qu?mico e atividades biol?gicas de Ouratea hexasperma var. planchonii Engl. (Ochnaceae) |
title_fullStr |
Estudo qu?mico e atividades biol?gicas de Ouratea hexasperma var. planchonii Engl. (Ochnaceae) |
title_full_unstemmed |
Estudo qu?mico e atividades biol?gicas de Ouratea hexasperma var. planchonii Engl. (Ochnaceae) |
title_sort |
Estudo qu?mico e atividades biol?gicas de Ouratea hexasperma var. planchonii Engl. (Ochnaceae) |
author |
Fernandes, Renata Duarte |
author_facet |
Fernandes, Renata Duarte |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Carvalho, Mario Geraldo de |
dc.contributor.referee1.fl_str_mv |
Carvalho, Mario Geraldo de |
dc.contributor.referee2.fl_str_mv |
Lima, Marco Edilson Freire de |
dc.contributor.referee3.fl_str_mv |
Werle, Alceni Augusta |
dc.contributor.referee4.fl_str_mv |
Carvalho, Geizi Jane Alves de |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/2500280502687742 |
dc.contributor.author.fl_str_mv |
Fernandes, Renata Duarte |
contributor_str_mv |
Carvalho, Mario Geraldo de Carvalho, Mario Geraldo de Lima, Marco Edilson Freire de Werle, Alceni Augusta Carvalho, Geizi Jane Alves de |
dc.subject.por.fl_str_mv |
Ouratea hexasperma flavon?ides atividades biol?gicas |
topic |
Ouratea hexasperma flavon?ides atividades biol?gicas flavonoids biologicals activities Qu?mica |
dc.subject.eng.fl_str_mv |
flavonoids biologicals activities |
dc.subject.cnpq.fl_str_mv |
Qu?mica |
description |
The specials metabolites isolated from the branches of the Ouratea hexasperma var. planchonii Engl., allowing to the comparison with the chemical constituents from variety Ouratea hexasperma (St. Hil.) to confirm the botanical classification. Some biological activities were evaluated. The analysis of the fractions from solvents and chromatographic fractionation of the dichloromethane and methanolic extracts from the branches of Ouratea hexasperma var. planchonii Engl. led to the identification of some chemical constituents. This procedure led to identification of aliphatics compounds mixture, aliphatic alcohol mixture, steroids mixture (sitosterol, stigmasterol and campesterol), three triterpenes, lupeol e -amiryn e -amiryn, and the biflavonoids (7?-O-methylagathisflavone and agathisflavone) and sitosterol-3-O- -D-glycopyranoside. The structures were determined by IR, de 1H (1D e 2D) e 13C (BBD e DEPT) NMR spectral data analysis and comparison with literature values. The HPLC-MS were used in the analysis of two fractions containing the these two biflavones and some additional compounds. This analysis led us to identify the presence of methyl-vitexin, orientin, methyl-dihydroxyflavone, glycopiranosil-flavones, dimethylagathisflavone and glycopyranosil-prenyl-dihydroxyflavonol besides the confirmation of these two biflavonoids. The extracts from the branches of Ouratea hexasperma var. planchonii Engl. were submitted on evaluations of the antibacterial, antifungal, insecticide and toxicity activities. The Escherichia coli and Staphylococcus aureus growth was inhibited with methanolic extract. The fractions containing the biflavonoids were partially active as insecticide against Musca domestica. The extracts presented low toxicity on unique dose. |
publishDate |
2008 |
dc.date.issued.fl_str_mv |
2008-07-11 |
dc.date.accessioned.fl_str_mv |
2020-08-24T21:14:28Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
FERNANDES, Renata Duarte. Estudo qu?mico e atividades biol?gicas de Ouratea hexasperma var. planchonii Engl. (Ochnaceae). 2008. 101 f. Disserta??o (Mestrado em Qu?mica Org?nica) - Instituto de Ci?ncias Exatas, Departamento de Qu?mica, Universidade Federal Rural do Rio de Janeiro, Serop?dica - RJ, 2008. |
dc.identifier.uri.fl_str_mv |
https://tede.ufrrj.br/jspui/handle/jspui/3842 |
identifier_str_mv |
FERNANDES, Renata Duarte. Estudo qu?mico e atividades biol?gicas de Ouratea hexasperma var. planchonii Engl. (Ochnaceae). 2008. 101 f. Disserta??o (Mestrado em Qu?mica Org?nica) - Instituto de Ci?ncias Exatas, Departamento de Qu?mica, Universidade Federal Rural do Rio de Janeiro, Serop?dica - RJ, 2008. |
url |
https://tede.ufrrj.br/jspui/handle/jspui/3842 |
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por |
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por |
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openAccess |
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Universidade Federal Rural do Rio de Janeiro |
dc.publisher.program.fl_str_mv |
Programa de P?s-Gradua??o em Qu?mica |
dc.publisher.initials.fl_str_mv |
UFRRJ |
dc.publisher.country.fl_str_mv |
Brasil |
dc.publisher.department.fl_str_mv |
Instituto de Ci?ncias Exatas |
publisher.none.fl_str_mv |
Universidade Federal Rural do Rio de Janeiro |
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