Xilanase e probi?tico em dietas para frangos de corte

Detalhes bibliográficos
Autor(a) principal: Machado, Noedson de Jesus Beltr?o
Data de Publicação: 2019
Tipo de documento: Tese
Idioma: por
Título da fonte: Biblioteca Digital de Teses e Dissertações da UFRRJ
Texto Completo: https://tede.ufrrj.br/jspui/handle/jspui/5209
Resumo: Broiler diets are generally composed of ingredients of plant origin, which may have high amounts of non-starch polysaccharides, which are considered antinutritional factors in broiler nutrition. Thus, the use of enzymes and probiotics can ensure greater availability of nutrients of plant origin minimizing the effects of NSP. Two experiments were carried out to study xylanase and probiotic supplementation in diets for broilers. Experiment I was carried at IFAM/Parintins, in an experimental shed, conducted in a completely randomized design with four treatments, with six replications of seven birds each. For the composition of the treatments, Colostrum Mix commercial products were used for the probiotic source and Smyzime Xylanase for the enzymatic source, and the enzyme was valued at 150 kcal/kg of feed. The treatments consisted of: reference diet; reference diet with probiotic addition; basal diet with xylanase addition and basal diet with xylanase addition and probiotic. The reference diet met all the nutritional requirements of the chickens, while the basal diet was formulated with 150 kcal/kg less metabolizable energy. Performance, carcass characteristics and intestinal viscosity were evaluated. Experiment II was performed in metal cages at UFAM/Manaus, conducted in a design and with treatments similar to that described above, and all diets were supplemented with wheat bran and birds challenged orally with commercial Eimeria vaccine administered 10x above recommendation. Performance, carcass, intestinal viscosity and pH and ileal microbiota were evaluated. In experiment I, the treatments influenced weight gain and feed intake, in which the isolated or combined supplementation of the additives did not differ, but led to greater gains in relation to the diet without supplementation from 1 to 35 days of age. Intestinal viscosity was significantly lower in treatment with xylanase alone (1.01 cP). In experiment II, from 10 to 35 days of age, isolated xylanase and probiotic bedding provided greater weight gain. The reference treatment presented higher viscosity in duodenum (1.42 cP) and ileum (1.21 cP), and higher duodenal pH value (6.54), with the probiotic diet resulting in lower pH value in the cecum (6 , 11). The ileal microbiota was influenced by the treatments, in which broilers fed the diet containing probiotic alone presented higher frequency of the genera Lactobacillus (94.20%). Xylanase and probiotic supplementation provided improvements in animal performance, intestinal viscosity and modulated the ileal microbiota. Combined feed additives in feed provided similar improvements to the isolated feed, so they had no associative effects.
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spelling Lima, Cristina Amorim Ribeiro deCPF: 449.983.176-87Curvello, Fernando AugustoVieites, Fl?vio MedeirosLima, Marcos F?bio deCardoso, Ver?nica da SilvaCPF: 003.386.952-90http://lattes.cnpq.br/7502454937805863Machado, Noedson de Jesus Beltr?o2021-10-31T00:21:37Z2019-10-11MACHADO, No?dson de Jesus Beltr?o. Xilanase e probi?tico em dietas para frangos de corte. 2019. 67 f. Tese (Doutorado em Zootecnia, Produ??o Animal). Instituto de Zootecnia, Universidade Federal Rural do Rio de Janeiro, Serop?dica, RJ, 2019.https://tede.ufrrj.br/jspui/handle/jspui/5209Broiler diets are generally composed of ingredients of plant origin, which may have high amounts of non-starch polysaccharides, which are considered antinutritional factors in broiler nutrition. Thus, the use of enzymes and probiotics can ensure greater availability of nutrients of plant origin minimizing the effects of NSP. Two experiments were carried out to study xylanase and probiotic supplementation in diets for broilers. Experiment I was carried at IFAM/Parintins, in an experimental shed, conducted in a completely randomized design with four treatments, with six replications of seven birds each. For the composition of the treatments, Colostrum Mix commercial products were used for the probiotic source and Smyzime Xylanase for the enzymatic source, and the enzyme was valued at 150 kcal/kg of feed. The treatments consisted of: reference diet; reference diet with probiotic addition; basal diet with xylanase addition and basal diet with xylanase addition and probiotic. The reference diet met all the nutritional requirements of the chickens, while the basal diet was formulated with 150 kcal/kg less metabolizable energy. Performance, carcass characteristics and intestinal viscosity were evaluated. Experiment II was performed in metal cages at UFAM/Manaus, conducted in a design and with treatments similar to that described above, and all diets were supplemented with wheat bran and birds challenged orally with commercial Eimeria vaccine administered 10x above recommendation. Performance, carcass, intestinal viscosity and pH and ileal microbiota were evaluated. In experiment I, the treatments influenced weight gain and feed intake, in which the isolated or combined supplementation of the additives did not differ, but led to greater gains in relation to the diet without supplementation from 1 to 35 days of age. Intestinal viscosity was significantly lower in treatment with xylanase alone (1.01 cP). In experiment II, from 10 to 35 days of age, isolated xylanase and probiotic bedding provided greater weight gain. The reference treatment presented higher viscosity in duodenum (1.42 cP) and ileum (1.21 cP), and higher duodenal pH value (6.54), with the probiotic diet resulting in lower pH value in the cecum (6 , 11). The ileal microbiota was influenced by the treatments, in which broilers fed the diet containing probiotic alone presented higher frequency of the genera Lactobacillus (94.20%). Xylanase and probiotic supplementation provided improvements in animal performance, intestinal viscosity and modulated the ileal microbiota. Combined feed additives in feed provided similar improvements to the isolated feed, so they had no associative effects.As dietas de frangos de corte s?o em geral constitu?das por ingredientes de origem vegetal, que podem apresentar elevada quantidade de polissacar?deos n?o amil?ceos, que s?o considerados fatores antinutricionais na nutri??o de frangos. Dessa forma, o uso de enzimas e probi?ticos podem assegurar maiores disponibilidades de nutrientes de origem vegetal minimizando os efeitos dos PNA?s. Foram realizados dois experimentos com o objetivo de estudar a suplementa??o de xilanase e probi?tico em dietas para frangos corte. O experimento I foi realizado no IFAM/Parintins, em galp?o experimental, conduzido em um delineamento inteiramente casualizado, com quatro tratamentos, com seis repeti??es de sete aves cada. Para a composi??o dos tratamentos foram uasados os produtos comerciais Colostrum Mix para a fonte do probi?tico e a Smyzime Xylanase para a fonte enzim?tica, sendo realizada valoriza??o da enzima em 150 kcal/kg de ra??o. Os tratamentos consistiram em: dieta refer?ncia; dieta refer?ncia com adi??o de probi?tico; dieta basal com adi??o de xilanase e dieta basal com adi??o de xilanase e probi?tico. A dieta refer?ncia atendia todas as exig?ncias nutricionais dos frangos, enquanto a dieta basal foi formulada com menos 150 kcal de energia metaboliz?vel por quilo de ra??o. Foram avaliados desempenho, carcater?sticas de carca?a e viscosidade intestinal. J? o experimento II, foi realizado em gaiolas met?licas, na UFAM/Manaus, conduzido em delinemanto e com tratamentos semelhantes ao descrito anteriormente, sendo que todas as dietas foram suplementadas com farelo de trigo e as aves desafiadas via oral com vacina comercial de Eimeria administrada 10x acima da recomenda??o. Foram avaliados, desempenho, carca?a, viscosidade e pH intestinal e microbiota ileal. No experimento I, os tratamentos influenciaram o ganho de peso e consumo de ra??o, em que a suplementa??o isolada ou combinada dos aditivos n?o diferiram, por?m conduziram a maiores ganhos em rela??o a dieta sem suplementa??o no per?odo de 1 a 35 dias de idade. A viscosidade intestinal foi significativamente menor no tratamento com xilanase fornecida isoladamente (1,01 cP). No experimento II, no per?odo de 10 a 35 dias de idade as deitas com xilanase e probi?tico de maneira isoladas proporcionaram maior ganho de peso. O tratamento refer?ncia apresentou maior viscosidade no duodeno (1,42 cP) e ?leo (1,21 cP), e maior valor de pH duodenal (6,54), sendo a dieta com probi?tico resultando em menor valor de pH no ceco (6,11). A microbiota ileal foi influenciada pelos tratamentos, em que os frangos alimentados com a dieta contendo probi?tico isoladamente apresentaram maior frequ?ncia do g?nero Lactobacillus (94,20%). A suplementa??o com xilanase e probi?tico proporcionaram melhorias no desempenho animal, viscosidade intestinal e modularam a microbiota ileal. Os aditivos fonecidos combinados na ra??o proprocionaram melhorias semelhantes ao fornecimento isolado, t?o logo, n?o exerceram efeitos associativos.Submitted by Jorge Silva (jorgelmsilva@ufrrj.br) on 2021-10-31T00:21:37Z No. of bitstreams: 1 2019 - No?dson de Jesus Beltr?o Machado.pdf: 1614934 bytes, checksum: 603244563f28b5c73b98a337a5039fd8 (MD5)Made available in DSpace on 2021-10-31T00:21:37Z (GMT). No. of bitstreams: 1 2019 - No?dson de Jesus Beltr?o Machado.pdf: 1614934 bytes, checksum: 603244563f28b5c73b98a337a5039fd8 (MD5) Previous issue date: 2019-10-11CAPES - Coordena??o de Aperfei?oamento de Pessoal de N?vel Superiorapplication/pdfhttps://tede.ufrrj.br/retrieve/67420/2019%20-%20No%c3%a9dson%20de%20Jesus%20Beltr%c3%a3o%20Machado.pdf.jpgporUniversidade Federal Rural do Rio de JaneiroPrograma de P?s-Gradua??o em ZootecniaUFRRJBrasilInstituto de ZootecniaABDEL-HAFEEZ, H. M.; SALEH, E. S.E.; TAWFEEK, S.S. et al. 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The origin of intrinsec ganglia of trunk viscera from vagal neural crest in the chick embryo. Journal of Comparative Neurology, v. 101, n. 12, p. 515?541, 1954. YU, B.; LIU, J.R.; HSIAO, F. S.; CHIOU, P. W. S. Evaluation of Lactobacillus reuteri Pg4 strain expressing heterologous -glucanase as a probiotic in poultry diets based on barley. Animal Feed Science and Technology, v. 141, p. 82?91, 2008. ZHANG, L.; L. ZHANG, X.; Zeng, L.; Zhou, G.; C.; YANG, C. Effects of dietary supplementation of probiotic, Clostridium butyricum, on growth performance, immune response, intestinal barrier function, and digestive enzyme activity in broiler chickens challenged with Escherichia coli K88. Journal of Animal Science and Biotechnology, v. 26, n. 7, p. 3. 2016. ZHOU, M.; ZENG, D.; NI, X. et al. Effects of Bacillus licheniformis on the growth performance and expression of lipid metabolism-related genes in broiler chickens challenged with Clostridium perfringens-induced necrotic enteritis. 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dc.title.por.fl_str_mv Xilanase e probi?tico em dietas para frangos de corte
dc.title.alternative.eng.fl_str_mv Xylanase and probiotic in broiler diets
title Xilanase e probi?tico em dietas para frangos de corte
spellingShingle Xilanase e probi?tico em dietas para frangos de corte
Machado, Noedson de Jesus Beltr?o
Aditivo
Enzima
Microbiota
Viscosidade
Additive
Enzyme
Microbiota
Viscosity
Zootecnia
title_short Xilanase e probi?tico em dietas para frangos de corte
title_full Xilanase e probi?tico em dietas para frangos de corte
title_fullStr Xilanase e probi?tico em dietas para frangos de corte
title_full_unstemmed Xilanase e probi?tico em dietas para frangos de corte
title_sort Xilanase e probi?tico em dietas para frangos de corte
author Machado, Noedson de Jesus Beltr?o
author_facet Machado, Noedson de Jesus Beltr?o
author_role author
dc.contributor.advisor1.fl_str_mv Lima, Cristina Amorim Ribeiro de
dc.contributor.advisor1ID.fl_str_mv CPF: 449.983.176-87
dc.contributor.referee1.fl_str_mv Curvello, Fernando Augusto
dc.contributor.referee2.fl_str_mv Vieites, Fl?vio Medeiros
dc.contributor.referee3.fl_str_mv Lima, Marcos F?bio de
dc.contributor.referee4.fl_str_mv Cardoso, Ver?nica da Silva
dc.contributor.authorID.fl_str_mv CPF: 003.386.952-90
dc.contributor.authorLattes.fl_str_mv http://lattes.cnpq.br/7502454937805863
dc.contributor.author.fl_str_mv Machado, Noedson de Jesus Beltr?o
contributor_str_mv Lima, Cristina Amorim Ribeiro de
Curvello, Fernando Augusto
Vieites, Fl?vio Medeiros
Lima, Marcos F?bio de
Cardoso, Ver?nica da Silva
dc.subject.por.fl_str_mv Aditivo
Enzima
Microbiota
Viscosidade
topic Aditivo
Enzima
Microbiota
Viscosidade
Additive
Enzyme
Microbiota
Viscosity
Zootecnia
dc.subject.eng.fl_str_mv Additive
Enzyme
Microbiota
Viscosity
dc.subject.cnpq.fl_str_mv Zootecnia
description Broiler diets are generally composed of ingredients of plant origin, which may have high amounts of non-starch polysaccharides, which are considered antinutritional factors in broiler nutrition. Thus, the use of enzymes and probiotics can ensure greater availability of nutrients of plant origin minimizing the effects of NSP. Two experiments were carried out to study xylanase and probiotic supplementation in diets for broilers. Experiment I was carried at IFAM/Parintins, in an experimental shed, conducted in a completely randomized design with four treatments, with six replications of seven birds each. For the composition of the treatments, Colostrum Mix commercial products were used for the probiotic source and Smyzime Xylanase for the enzymatic source, and the enzyme was valued at 150 kcal/kg of feed. The treatments consisted of: reference diet; reference diet with probiotic addition; basal diet with xylanase addition and basal diet with xylanase addition and probiotic. The reference diet met all the nutritional requirements of the chickens, while the basal diet was formulated with 150 kcal/kg less metabolizable energy. Performance, carcass characteristics and intestinal viscosity were evaluated. Experiment II was performed in metal cages at UFAM/Manaus, conducted in a design and with treatments similar to that described above, and all diets were supplemented with wheat bran and birds challenged orally with commercial Eimeria vaccine administered 10x above recommendation. Performance, carcass, intestinal viscosity and pH and ileal microbiota were evaluated. In experiment I, the treatments influenced weight gain and feed intake, in which the isolated or combined supplementation of the additives did not differ, but led to greater gains in relation to the diet without supplementation from 1 to 35 days of age. Intestinal viscosity was significantly lower in treatment with xylanase alone (1.01 cP). In experiment II, from 10 to 35 days of age, isolated xylanase and probiotic bedding provided greater weight gain. The reference treatment presented higher viscosity in duodenum (1.42 cP) and ileum (1.21 cP), and higher duodenal pH value (6.54), with the probiotic diet resulting in lower pH value in the cecum (6 , 11). The ileal microbiota was influenced by the treatments, in which broilers fed the diet containing probiotic alone presented higher frequency of the genera Lactobacillus (94.20%). Xylanase and probiotic supplementation provided improvements in animal performance, intestinal viscosity and modulated the ileal microbiota. Combined feed additives in feed provided similar improvements to the isolated feed, so they had no associative effects.
publishDate 2019
dc.date.issued.fl_str_mv 2019-10-11
dc.date.accessioned.fl_str_mv 2021-10-31T00:21:37Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/doctoralThesis
format doctoralThesis
status_str publishedVersion
dc.identifier.citation.fl_str_mv MACHADO, No?dson de Jesus Beltr?o. Xilanase e probi?tico em dietas para frangos de corte. 2019. 67 f. Tese (Doutorado em Zootecnia, Produ??o Animal). Instituto de Zootecnia, Universidade Federal Rural do Rio de Janeiro, Serop?dica, RJ, 2019.
dc.identifier.uri.fl_str_mv https://tede.ufrrj.br/jspui/handle/jspui/5209
identifier_str_mv MACHADO, No?dson de Jesus Beltr?o. Xilanase e probi?tico em dietas para frangos de corte. 2019. 67 f. Tese (Doutorado em Zootecnia, Produ??o Animal). Instituto de Zootecnia, Universidade Federal Rural do Rio de Janeiro, Serop?dica, RJ, 2019.
url https://tede.ufrrj.br/jspui/handle/jspui/5209
dc.language.iso.fl_str_mv por
language por
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dc.publisher.none.fl_str_mv Universidade Federal Rural do Rio de Janeiro
dc.publisher.program.fl_str_mv Programa de P?s-Gradua??o em Zootecnia
dc.publisher.initials.fl_str_mv UFRRJ
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