Biodiversidade de esp?cies de Diplostomoidea (Trematoda: Digenea: Diplostomida) em peixes de ?gua doce no Brasil: uma abordagem integrativa

• Autor(a) principal: Taborda, Naraiana Lopes
• Data de Publicação: 2020
• Tipo de documento: Tese
• Idioma: por
• Título da fonte: Biblioteca Digital de Teses e Dissertações da UFRRJ
• Texto Completo: https://tede.ufrrj.br/jspui/handle/jspui/5916
• topic: Diplostomoidea; Associa??o parasito-hospedeiro; Taxonomia integrativa; Host-parasite associations; Integrative taxonomy; Medicina Veterin?ria

The present study uses an integrative approach to understanding the diverity of diplostomoids in Brazil. This project aimed to study the biodiversity of diplostomoids in Brazil and to shed light on the complex links between hosts and parasites. The lack of parameters and notoriously difficult identification of the metacercarial larval stages represented the main challenge accomplishing the present work. Sampling of freshwater fish were carried out in regions of differentes biomes with important Brazilian biodiversity as Brazilian Amazon, Pantanal and Atlantic Fores, including 11 localities and a total of 1,140 fish from 27 families. In the course of the stduy we profit from the opportunity to obtain material from reptile and bird definitive hosts which enable partial life-cycle elusidation of one proterodiplostomid (Prolecithodiplostomum constrictum Dubois, 1936) and three new species of Posthodiplostomum Dubois, 1936. The material collected resulted in the generating of sequence libraries (linking sequences for the mitochondrial cythochrom c oxidase subunit 1 gene and the nuclear 28S rDNA) for the South American diplostomoid which were virtually missing and will serve as a base for future diversity assessmnets. The generated morphological and sequence reference libraries substantially complemented the existing ones on the the group from North America, Europe and Africa. Results are summarised in two chapters focused on: (i) proterodiplostomid diversity in the Brazilian freshwater reporting on eight speies of which six putative new and further providing an improved resolution on the Diplostomoidea with a non-basal positioning of members of Proterodiplostomidae and re-validation of the type ?proterodiplostomulum? metacercaria on the group; and (ii) black-sprot disease pathogens in fishes providing data for eigth species recovered in fish and bird hosts, and highlighting the importance of the use of an intergrative approach for species identification linking different lines of evicence gathered from morphology and molecular of parasites. Therefore, the results are discussed in terms of species diversity and diverisification processes, endemism and host-parasite associations. The present study emphasizes the need to further our knowledge not only on the Neotropical trematode parasite diversity but this at global.