Analysis of genetic diversity of a native population of Myrcia lundiana Kiaersk. plants using ISSR markers
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Publication Date: | 2016 |
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Format: | Article |
Language: | eng |
Source: | Repositório Institucional da UFS |
Download full: | http://ri.ufs.br/jspui/handle/riufs/7288 |
Summary: | Myrcia lundiana Kiaersk. is a tree of the family Myrtaceae found in tropical and subtropical areas of the southern hemisphere that produces essential oil. The aim of this study was to characterize the genetic diversity of M. lundiana plants from a native population of Parque Nacional de Itabaiana, using inter-simple sequence repeat molecular markers. Thirty-five primers were tested, 20 of which were polymorphic, resulting in 135 polymorphic and informative bands. Results of the cluster analysis, obtained using the unweighted pair group method with arithmetic mean, grouped plants into three clusters: Cluster I - MLU001, MLU002, MLU003, MLU004, MLU005, MLU006, MLU018, MLU019, MLU020, MLU021, MLU022; MLU008, MLU011, MLU012, MLU014, MLU015, MLU017, MLU026, and MLU028; Cluster II - MLU007, MLU009, MLU010, MLU013, and MLU016; and Cluster III - MLU023, MLU024, MLU025, and MLU027. Jaccard similarity coefficients for pair-wise comparisons of plants ranged between 0.15 and 0.87. MLU014 and MLU015 presented low genetic diversity, with a similarity index of 0.87. Conversely, MLU007 and MLU019 presented high diversity, with a similarity index of 0.15. According to the structure analysis, three distinct clusters were formed. Genetic diversity of M. lundiana plants was intermediate, and expansion of its genetic diversity is necessary. MLU026 and MLU028 are the most suitable for selection in breeding programs, since they clearly represent all of the diversity present in these plants. Moreover, these results provide important information on the existing genetic variability, highlighting the importance of Parque Nacional de Itabaiana for the conservation of this species. |
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Alves, Mercia FreitasNizio, Daniela Aparecida de CastroBrito, Fabiany de AndradeSampaio, Taís SantosMuniz, Ana Veruska Cruz da SilvaArrigoni-Blank, Maria de FátimaCarvalho, Sheila Valéria ÁlvaresBlank, Arie Fitzgerald2018-02-01T13:24:56Z2018-02-01T13:24:56Z2016ALVES, M. F. et al. Analysis of genetic diversity of a native population of Myrcia lundiana Kiaersk. plants using ISSR markers. Genetics and Molecular Research, Ribeirão Preto, v. 15, n. 4, 2016. Disponível em: <https://www.geneticsmr.com/abstract/analysis-of-genetic-diversity-of-a-native-population-of-myrcia-lundiana-kiaersk-plants-using-issr-markers-2090.html>. Acesso em: 01 fev. 2018.1676-5680http://ri.ufs.br/jspui/handle/riufs/7288Creative Commons Atribuição-Não Comercial-Compartilha Igual 4.0 Internacional (CC BY-NC-SA 4.0)engFundação de Pesquisas Científicas de Ribeirão Preto (FUNPEC-RP)Genetics and Molecular ResearchMyrcia lundianaConservationGenetic diversityISSRConservaçãoDiversidade genéticaAnalysis of genetic diversity of a native population of Myrcia lundiana Kiaersk. plants using ISSR markersinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleMyrcia lundiana Kiaersk. is a tree of the family Myrtaceae found in tropical and subtropical areas of the southern hemisphere that produces essential oil. The aim of this study was to characterize the genetic diversity of M. lundiana plants from a native population of Parque Nacional de Itabaiana, using inter-simple sequence repeat molecular markers. Thirty-five primers were tested, 20 of which were polymorphic, resulting in 135 polymorphic and informative bands. Results of the cluster analysis, obtained using the unweighted pair group method with arithmetic mean, grouped plants into three clusters: Cluster I - MLU001, MLU002, MLU003, MLU004, MLU005, MLU006, MLU018, MLU019, MLU020, MLU021, MLU022; MLU008, MLU011, MLU012, MLU014, MLU015, MLU017, MLU026, and MLU028; Cluster II - MLU007, MLU009, MLU010, MLU013, and MLU016; and Cluster III - MLU023, MLU024, MLU025, and MLU027. Jaccard similarity coefficients for pair-wise comparisons of plants ranged between 0.15 and 0.87. MLU014 and MLU015 presented low genetic diversity, with a similarity index of 0.87. Conversely, MLU007 and MLU019 presented high diversity, with a similarity index of 0.15. According to the structure analysis, three distinct clusters were formed. Genetic diversity of M. lundiana plants was intermediate, and expansion of its genetic diversity is necessary. MLU026 and MLU028 are the most suitable for selection in breeding programs, since they clearly represent all of the diversity present in these plants. Moreover, these results provide important information on the existing genetic variability, highlighting the importance of Parque Nacional de Itabaiana for the conservation of this species.reponame:Repositório Institucional da UFSinstname:Universidade Federal de Sergipe (UFS)instacron:UFSinfo:eu-repo/semantics/openAccessLICENSElicense.txtlicense.txttext/plain; charset=utf-81475https://ri.ufs.br/jspui/bitstream/riufs/7288/1/license.txt098cbbf65c2c15e1fb2e49c5d306a44cMD51ORIGINALMyrciaLundianaKiaerskISSRMarkers.pdfMyrciaLundianaKiaerskISSRMarkers.pdfapplication/pdf843190https://ri.ufs.br/jspui/bitstream/riufs/7288/2/MyrciaLundianaKiaerskISSRMarkers.pdf7ea2d6279f004ac4ba98862b397b730aMD52TEXTMyrciaLundianaKiaerskISSRMarkers.pdf.txtMyrciaLundianaKiaerskISSRMarkers.pdf.txtExtracted texttext/plain27957https://ri.ufs.br/jspui/bitstream/riufs/7288/3/MyrciaLundianaKiaerskISSRMarkers.pdf.txtb7344efb0b0a368e94e883e38cbb75e5MD53THUMBNAILMyrciaLundianaKiaerskISSRMarkers.pdf.jpgMyrciaLundianaKiaerskISSRMarkers.pdf.jpgGenerated Thumbnailimage/jpeg1587https://ri.ufs.br/jspui/bitstream/riufs/7288/4/MyrciaLundianaKiaerskISSRMarkers.pdf.jpgd845d7188562f0491b3158e9fabe6b17MD54riufs/72882018-02-01 10:24:57.111oai:ufs.br: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Repositório InstitucionalPUBhttps://ri.ufs.br/oai/requestrepositorio@academico.ufs.bropendoar:2018-02-01T13:24:57Repositório Institucional da UFS - Universidade Federal de Sergipe (UFS)false |
dc.title.pt_BR.fl_str_mv |
Analysis of genetic diversity of a native population of Myrcia lundiana Kiaersk. plants using ISSR markers |
title |
Analysis of genetic diversity of a native population of Myrcia lundiana Kiaersk. plants using ISSR markers |
spellingShingle |
Analysis of genetic diversity of a native population of Myrcia lundiana Kiaersk. plants using ISSR markers Alves, Mercia Freitas Myrcia lundiana Conservation Genetic diversity ISSR Conservação Diversidade genética |
title_short |
Analysis of genetic diversity of a native population of Myrcia lundiana Kiaersk. plants using ISSR markers |
title_full |
Analysis of genetic diversity of a native population of Myrcia lundiana Kiaersk. plants using ISSR markers |
title_fullStr |
Analysis of genetic diversity of a native population of Myrcia lundiana Kiaersk. plants using ISSR markers |
title_full_unstemmed |
Analysis of genetic diversity of a native population of Myrcia lundiana Kiaersk. plants using ISSR markers |
title_sort |
Analysis of genetic diversity of a native population of Myrcia lundiana Kiaersk. plants using ISSR markers |
author |
Alves, Mercia Freitas |
author_facet |
Alves, Mercia Freitas Nizio, Daniela Aparecida de Castro Brito, Fabiany de Andrade Sampaio, Taís Santos Muniz, Ana Veruska Cruz da Silva Arrigoni-Blank, Maria de Fátima Carvalho, Sheila Valéria Álvares Blank, Arie Fitzgerald |
author_role |
author |
author2 |
Nizio, Daniela Aparecida de Castro Brito, Fabiany de Andrade Sampaio, Taís Santos Muniz, Ana Veruska Cruz da Silva Arrigoni-Blank, Maria de Fátima Carvalho, Sheila Valéria Álvares Blank, Arie Fitzgerald |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Alves, Mercia Freitas Nizio, Daniela Aparecida de Castro Brito, Fabiany de Andrade Sampaio, Taís Santos Muniz, Ana Veruska Cruz da Silva Arrigoni-Blank, Maria de Fátima Carvalho, Sheila Valéria Álvares Blank, Arie Fitzgerald |
dc.subject.eng.fl_str_mv |
Myrcia lundiana Conservation Genetic diversity ISSR |
topic |
Myrcia lundiana Conservation Genetic diversity ISSR Conservação Diversidade genética |
dc.subject.por.fl_str_mv |
Conservação Diversidade genética |
description |
Myrcia lundiana Kiaersk. is a tree of the family Myrtaceae found in tropical and subtropical areas of the southern hemisphere that produces essential oil. The aim of this study was to characterize the genetic diversity of M. lundiana plants from a native population of Parque Nacional de Itabaiana, using inter-simple sequence repeat molecular markers. Thirty-five primers were tested, 20 of which were polymorphic, resulting in 135 polymorphic and informative bands. Results of the cluster analysis, obtained using the unweighted pair group method with arithmetic mean, grouped plants into three clusters: Cluster I - MLU001, MLU002, MLU003, MLU004, MLU005, MLU006, MLU018, MLU019, MLU020, MLU021, MLU022; MLU008, MLU011, MLU012, MLU014, MLU015, MLU017, MLU026, and MLU028; Cluster II - MLU007, MLU009, MLU010, MLU013, and MLU016; and Cluster III - MLU023, MLU024, MLU025, and MLU027. Jaccard similarity coefficients for pair-wise comparisons of plants ranged between 0.15 and 0.87. MLU014 and MLU015 presented low genetic diversity, with a similarity index of 0.87. Conversely, MLU007 and MLU019 presented high diversity, with a similarity index of 0.15. According to the structure analysis, three distinct clusters were formed. Genetic diversity of M. lundiana plants was intermediate, and expansion of its genetic diversity is necessary. MLU026 and MLU028 are the most suitable for selection in breeding programs, since they clearly represent all of the diversity present in these plants. Moreover, these results provide important information on the existing genetic variability, highlighting the importance of Parque Nacional de Itabaiana for the conservation of this species. |
publishDate |
2016 |
dc.date.issued.fl_str_mv |
2016 |
dc.date.accessioned.fl_str_mv |
2018-02-01T13:24:56Z |
dc.date.available.fl_str_mv |
2018-02-01T13:24:56Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
ALVES, M. F. et al. Analysis of genetic diversity of a native population of Myrcia lundiana Kiaersk. plants using ISSR markers. Genetics and Molecular Research, Ribeirão Preto, v. 15, n. 4, 2016. Disponível em: <https://www.geneticsmr.com/abstract/analysis-of-genetic-diversity-of-a-native-population-of-myrcia-lundiana-kiaersk-plants-using-issr-markers-2090.html>. Acesso em: 01 fev. 2018. |
dc.identifier.uri.fl_str_mv |
http://ri.ufs.br/jspui/handle/riufs/7288 |
dc.identifier.issn.none.fl_str_mv |
1676-5680 |
dc.identifier.license.pt_BR.fl_str_mv |
Creative Commons Atribuição-Não Comercial-Compartilha Igual 4.0 Internacional (CC BY-NC-SA 4.0) |
identifier_str_mv |
ALVES, M. F. et al. Analysis of genetic diversity of a native population of Myrcia lundiana Kiaersk. plants using ISSR markers. Genetics and Molecular Research, Ribeirão Preto, v. 15, n. 4, 2016. Disponível em: <https://www.geneticsmr.com/abstract/analysis-of-genetic-diversity-of-a-native-population-of-myrcia-lundiana-kiaersk-plants-using-issr-markers-2090.html>. Acesso em: 01 fev. 2018. 1676-5680 Creative Commons Atribuição-Não Comercial-Compartilha Igual 4.0 Internacional (CC BY-NC-SA 4.0) |
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http://ri.ufs.br/jspui/handle/riufs/7288 |
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eng |
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eng |
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Genetics and Molecular Research |
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openAccess |
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Fundação de Pesquisas Científicas de Ribeirão Preto (FUNPEC-RP) |
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