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Detalhes bibliográficos
Autor(a) principal: Carvalho, Ana Flávia de
Data de Publicação: 2007
Outros Autores: Mançanares, Celina Almeida Furlanetto, Pereira, Flávia Thomaz Verechia, Ambrósio, Carlos Eduardo, Miglino, Maria Angélica
Tipo de documento: Artigo
Idioma: por
Título da fonte: Biotemas (Online)
Texto Completo: https://periodicos.ufsc.br/index.php/biotemas/article/view/20735
Resumo: Immunolocalization of progesterone receptors in binucleate trophoblast cells of the buffalo placenta (Bubalus bubalis). The binucleate trophoblast cells (CTBs) of the water buffalo placenta (Bubalus bubalis) were studied with emphasis on the presence of progesterone receptor. Placentomal tissues from 27 buffalos (2-10 months of pregnancy) were processed and embedded in paraplast (Paraplast Embedding Media - Paraplast Plus) to locate the progesterone receptors using the immunohistochemistry technique. The immunohistochemical reaction for progesterone receptor through monoclonal antibody PgR Ab2 showed staining of CTBs, caruncular epithelial and estromal cells and blood vessel estromal pericitos present in the placentome throughout the entire gestational period analyzed. These results indicate the production of progesterone with autocrine and paracrine action in the placentome growth, differentiation and functional regulation.
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spelling .Imunolocalização de receptores de progesterona nas células trofoblásticas binucleadas na placenta de búfalo (Bubalus bubalis).Immunolocalization of progesterone receptors in binucleate trophoblast cells of the buffalo placenta (Bubalus bubalis). The binucleate trophoblast cells (CTBs) of the water buffalo placenta (Bubalus bubalis) were studied with emphasis on the presence of progesterone receptor. Placentomal tissues from 27 buffalos (2-10 months of pregnancy) were processed and embedded in paraplast (Paraplast Embedding Media - Paraplast Plus) to locate the progesterone receptors using the immunohistochemistry technique. The immunohistochemical reaction for progesterone receptor through monoclonal antibody PgR Ab2 showed staining of CTBs, caruncular epithelial and estromal cells and blood vessel estromal pericitos present in the placentome throughout the entire gestational period analyzed. These results indicate the production of progesterone with autocrine and paracrine action in the placentome growth, differentiation and functional regulation.As células trofoblásticas binucleadas (CTBs) da placenta de búfalos (Bubalus bubalis), foram estudadas, com ênfase à presença de receptores de progesterona. Tecidos placentomais de 27 búfalas (2-10 meses deprenhez) foram processados e embebidos em paraplast (Paraplast Embedding Media – Paraplast Plus) para localização de receptores de progesterona, utilizando-se a técnica de imunohistoquímica. A reação de imunohistoquímica para receptores de progesterona através de anticorpo monoclonal PgR Ab2 mostrou marcação de CTBs, células do epitélio e do estroma caruncular, e pericitos de vasos presentes nos placentônios ao longo de todo o período gestacional analisado. Os resultados indicam a produção de progesterona com ação autócrina e parácrina no crescimento, diferenciação e regulação funcional.Universidade Federal de Santa Catarina2007-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://periodicos.ufsc.br/index.php/biotemas/article/view/20735Biotemas; v. 20 n. 2 (2007); 99-1062175-79250103-1643reponame:Biotemas (Online)instname:Universidade Federal de Santa Catarina (UFSC)instacron:UFSCporhttps://periodicos.ufsc.br/index.php/biotemas/article/view/20735/18859Copyright (c) 2007 Ana Flávia de Carvalho, Celina Almeida Furlanetto Mançanares, Flávia Thomaz Verechia Pereira, Carlos Eduardo Ambrósio, Maria Angélica Miglinohttp://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessCarvalho, Ana Flávia deMançanares, Celina Almeida FurlanettoPereira, Flávia Thomaz VerechiaAmbrósio, Carlos EduardoMiglino, Maria Angélica2022-11-21T14:14:58Zoai:periodicos.ufsc.br:article/20735Revistahttp://www.biotemas.ufsc.br/index.htmPUBhttps://periodicos.ufsc.br/index.php/biotemas/oai||carlospinto@ccb.ufsc.br2175-79250103-1643opendoar:2022-11-21T14:14:58Biotemas (Online) - Universidade Federal de Santa Catarina (UFSC)false
dc.title.none.fl_str_mv .
Imunolocalização de receptores de progesterona nas células trofoblásticas binucleadas na placenta de búfalo (Bubalus bubalis).
title .
spellingShingle .
Carvalho, Ana Flávia de
title_short .
title_full .
title_fullStr .
title_full_unstemmed .
title_sort .
author Carvalho, Ana Flávia de
author_facet Carvalho, Ana Flávia de
Mançanares, Celina Almeida Furlanetto
Pereira, Flávia Thomaz Verechia
Ambrósio, Carlos Eduardo
Miglino, Maria Angélica
author_role author
author2 Mançanares, Celina Almeida Furlanetto
Pereira, Flávia Thomaz Verechia
Ambrósio, Carlos Eduardo
Miglino, Maria Angélica
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Carvalho, Ana Flávia de
Mançanares, Celina Almeida Furlanetto
Pereira, Flávia Thomaz Verechia
Ambrósio, Carlos Eduardo
Miglino, Maria Angélica
description Immunolocalization of progesterone receptors in binucleate trophoblast cells of the buffalo placenta (Bubalus bubalis). The binucleate trophoblast cells (CTBs) of the water buffalo placenta (Bubalus bubalis) were studied with emphasis on the presence of progesterone receptor. Placentomal tissues from 27 buffalos (2-10 months of pregnancy) were processed and embedded in paraplast (Paraplast Embedding Media - Paraplast Plus) to locate the progesterone receptors using the immunohistochemistry technique. The immunohistochemical reaction for progesterone receptor through monoclonal antibody PgR Ab2 showed staining of CTBs, caruncular epithelial and estromal cells and blood vessel estromal pericitos present in the placentome throughout the entire gestational period analyzed. These results indicate the production of progesterone with autocrine and paracrine action in the placentome growth, differentiation and functional regulation.
publishDate 2007
dc.date.none.fl_str_mv 2007-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://periodicos.ufsc.br/index.php/biotemas/article/view/20735
url https://periodicos.ufsc.br/index.php/biotemas/article/view/20735
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://periodicos.ufsc.br/index.php/biotemas/article/view/20735/18859
dc.rights.driver.fl_str_mv http://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Federal de Santa Catarina
publisher.none.fl_str_mv Universidade Federal de Santa Catarina
dc.source.none.fl_str_mv Biotemas; v. 20 n. 2 (2007); 99-106
2175-7925
0103-1643
reponame:Biotemas (Online)
instname:Universidade Federal de Santa Catarina (UFSC)
instacron:UFSC
instname_str Universidade Federal de Santa Catarina (UFSC)
instacron_str UFSC
institution UFSC
reponame_str Biotemas (Online)
collection Biotemas (Online)
repository.name.fl_str_mv Biotemas (Online) - Universidade Federal de Santa Catarina (UFSC)
repository.mail.fl_str_mv ||carlospinto@ccb.ufsc.br
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