Efeito hipolipidêmico e hepatoprotetor do 4,4’-dicloro-difenil disseleneto em um modelo de hiperlipidemia em ratos
Autor(a) principal: | |
---|---|
Data de Publicação: | 2016 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Manancial - Repositório Digital da UFSM |
Texto Completo: | http://repositorio.ufsm.br/handle/1/17339 |
Resumo: | Hyperlipidemia can be manifested by elevation in total cholesterol (TC), triglycerides (TGs) and low density lipoprotein (LDL), as well as by reduction in high density lipoprotein (HDL) levels. Hyperlipidemia is associated with oxidative stress and endothelial damage followed by atheroma and cardiovascular disease (CVDs) development. Moreover, high lipid levels are related with non alcoholic fatty liver disease (NAFLD). In view of the limitations and side effects of current drugs to treat hyperlipidemia, it becomes interesting to search for new drugs with hypolipidemic and hepatoprotective effects. The purpose of this study was to investigate the possible hepatoprotective and antihyperlipidemic effects of 4,4'-dichlorodiphenyl diselenide [(p-ClPhSe)2] in a hyperlipidemia model induced by Triton WR-1339 in rats. Biochemical analyses and hepatic oxidative stress parameters were evaluated in rats exposed to triton WR-1339 (400 mg/kg; i.p.) and treated with (p-ClPhSe)2 (10 mg/kg; i.g.) for seven days. After triton WR-1339 injection and fasting for 18 hours, the animals were exposed to the activity monitor for evaluation of exploratory and locomotor activity. Subsequently, blood was collected for determining biochemical parameters. The levels of TC, TGs, non-HDL-cholesterol (non-HDL), coronary risk index (CRI), reactive oxygen species, alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activities were significantly increased in triton treated rats. (p-ClPhSe)2 treatment resulted in a significant decrease in plasma lipid levels and was effective in normalizing the enzyme activities. (p-ClPhSe)2 did not protect against the increase of ROS levels, but increased NPHS levels in triton treated animals. (p-ClPhSe)2 could have potential in hyperlipidemia treatment as well as to reduce liver damage caused by this disorder. |
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2019-07-05T21:46:57Z2019-07-05T21:46:57Z2016-02-29http://repositorio.ufsm.br/handle/1/17339Hyperlipidemia can be manifested by elevation in total cholesterol (TC), triglycerides (TGs) and low density lipoprotein (LDL), as well as by reduction in high density lipoprotein (HDL) levels. Hyperlipidemia is associated with oxidative stress and endothelial damage followed by atheroma and cardiovascular disease (CVDs) development. Moreover, high lipid levels are related with non alcoholic fatty liver disease (NAFLD). In view of the limitations and side effects of current drugs to treat hyperlipidemia, it becomes interesting to search for new drugs with hypolipidemic and hepatoprotective effects. The purpose of this study was to investigate the possible hepatoprotective and antihyperlipidemic effects of 4,4'-dichlorodiphenyl diselenide [(p-ClPhSe)2] in a hyperlipidemia model induced by Triton WR-1339 in rats. Biochemical analyses and hepatic oxidative stress parameters were evaluated in rats exposed to triton WR-1339 (400 mg/kg; i.p.) and treated with (p-ClPhSe)2 (10 mg/kg; i.g.) for seven days. After triton WR-1339 injection and fasting for 18 hours, the animals were exposed to the activity monitor for evaluation of exploratory and locomotor activity. Subsequently, blood was collected for determining biochemical parameters. The levels of TC, TGs, non-HDL-cholesterol (non-HDL), coronary risk index (CRI), reactive oxygen species, alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activities were significantly increased in triton treated rats. (p-ClPhSe)2 treatment resulted in a significant decrease in plasma lipid levels and was effective in normalizing the enzyme activities. (p-ClPhSe)2 did not protect against the increase of ROS levels, but increased NPHS levels in triton treated animals. (p-ClPhSe)2 could have potential in hyperlipidemia treatment as well as to reduce liver damage caused by this disorder.A hiperlipidemia pode ser manifestada pela elevação de colesterol total (CT), triglicerídeos (TGs) e lipoproteínas de baixa densidade (LDL), bem como pela diminuição de lipoproteínas de alta densidade (HDL) no soro. A hiperlipidemia está associada com o estresse oxidativo e dano endotelial, seguido de ateromas e desenvolvimento de doenças cardiovasculares (DCs). Além disso, elevados níveis lipídicos estão relacionados com o desenvolvimento de Doença Hepática Gordurosa Não Alcoólica (DHGNA). Devido às limitações e efeitos colaterais dos fármacos existentes atualmente para o tratamento de dislipidemias, torna-se interessante a busca por novas drogas com potencial hipolipidêmico e hepatoprotetor. O objetivo deste trabalho foi avaliar os possíveis efeitos protetores do 4,4’-dicloro-difenil disseleneto [(p-ClPhSe)2] sobre a ação hiperlipidêmica e hepatotóxica induzida por Triton WR – 1339 em ratos. Para esta proposta, ratos Wistar foram tratados diariamente, durante sete dias, pela via intragástrica com (p-ClPhSe)2 na dose de 10 mg/kg ou óleo mineral (veículo). No 7º dia, trinta minutos após a última administração, os animais receberam salina (1 ml/kg, intraperitoneal, i.p.) ou triton WR-1339 (400 mg/kg, 1 ml/kg, i.p.). Após jejum de 18h, os animais foram expostos ao monitor de atividades para avaliação da atividade exploratória e locomotora. Subsequentemente, o sangue foi coletado para realização de dosagens bioquímicas. Os níveis de CT, TG, colesterol não-HDL, índice de risco coronariano, espécies reativas de oxigênio (EROS), alanina aminotransferase (ALT) e aspartato aminotransferase (AST) foram significativamente aumentados em ratos administrados com triton WR-1339. O tratamento com (p-ClPhSe)2 resultou em significativo decréscimo dos parâmetros lipídicos e enzimáticos alterados por triton WR-1330. O (p-ClPhSe)2 não foi eficaz em proteger do aumento de EROS, porém os animais tratados com o composto orgânico de selênio apresentaram aumento nos níveis de tióis não proteicos (NPSH). O (p-ClPhSe)2 apresentou efeito promissor em atenuar a hiperlipidemia e danos hepáticos induzidos por triton WR-1339.Conselho Nacional de Pesquisa e Desenvolvimento Científico e Tecnológico - CNPqporUniversidade Federal de Santa MariaCentro de Ciências Naturais e ExatasPrograma de Pós-Graduação em Ciências Biológicas: Bioquímica ToxicológicaUFSMBrasilBioquímicaAttribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessCompostos orgânicos de selênioDislipidemiaHepatotoxicidadeTriton WR – 1339RatosOrganoselenium compoundDyslipidemiaHepatotoxicityRatsCNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICAEfeito hipolipidêmico e hepatoprotetor do 4,4’-dicloro-difenil disseleneto em um modelo de hiperlipidemia em ratosHypolipidemic and hepatoprotective effect of 4,4'-dichloro-diphenyl diselenide in a hiperlipidemic model in ratsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisZeni, Gilson Rogériohttp://lattes.cnpq.br/2355575631197937Rocha, Juliana Trevisan dahttp://lattes.cnpq.br/2173530592429464Morsch, Vera Maria Melchiorshttp://lattes.cnpq.br/1519648219507868http://lattes.cnpq.br/0273230600642926Heck, Suélen Osório200800000002600b21a898a-0ab0-4f33-b980-2b55ef590b94ab6d6ca4-db36-4d6b-8e98-111edf0ba215d695948c-c58e-492a-a375-43addb8c9a3c1d69ed78-d2ea-4803-97d9-3f8b5483c10dreponame:Manancial - Repositório Digital da UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSMORIGINALDIS_PPGBT_2016_HECK_SUELEN.pdfDIS_PPGBT_2016_HECK_SUELEN.pdfDissertação de Mestradoapplication/pdf986101http://repositorio.ufsm.br/bitstream/1/17339/1/DIS_PPGBT_2016_HECK_SUELEN.pdfdfa176c0815626d72fa630a9168e67afMD51CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; 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dc.title.por.fl_str_mv |
Efeito hipolipidêmico e hepatoprotetor do 4,4’-dicloro-difenil disseleneto em um modelo de hiperlipidemia em ratos |
dc.title.alternative.eng.fl_str_mv |
Hypolipidemic and hepatoprotective effect of 4,4'-dichloro-diphenyl diselenide in a hiperlipidemic model in rats |
title |
Efeito hipolipidêmico e hepatoprotetor do 4,4’-dicloro-difenil disseleneto em um modelo de hiperlipidemia em ratos |
spellingShingle |
Efeito hipolipidêmico e hepatoprotetor do 4,4’-dicloro-difenil disseleneto em um modelo de hiperlipidemia em ratos Heck, Suélen Osório Compostos orgânicos de selênio Dislipidemia Hepatotoxicidade Triton WR – 1339 Ratos Organoselenium compound Dyslipidemia Hepatotoxicity Rats CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA |
title_short |
Efeito hipolipidêmico e hepatoprotetor do 4,4’-dicloro-difenil disseleneto em um modelo de hiperlipidemia em ratos |
title_full |
Efeito hipolipidêmico e hepatoprotetor do 4,4’-dicloro-difenil disseleneto em um modelo de hiperlipidemia em ratos |
title_fullStr |
Efeito hipolipidêmico e hepatoprotetor do 4,4’-dicloro-difenil disseleneto em um modelo de hiperlipidemia em ratos |
title_full_unstemmed |
Efeito hipolipidêmico e hepatoprotetor do 4,4’-dicloro-difenil disseleneto em um modelo de hiperlipidemia em ratos |
title_sort |
Efeito hipolipidêmico e hepatoprotetor do 4,4’-dicloro-difenil disseleneto em um modelo de hiperlipidemia em ratos |
author |
Heck, Suélen Osório |
author_facet |
Heck, Suélen Osório |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Zeni, Gilson Rogério |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/2355575631197937 |
dc.contributor.referee1.fl_str_mv |
Rocha, Juliana Trevisan da |
dc.contributor.referee1Lattes.fl_str_mv |
http://lattes.cnpq.br/2173530592429464 |
dc.contributor.referee2.fl_str_mv |
Morsch, Vera Maria Melchiors |
dc.contributor.referee2Lattes.fl_str_mv |
http://lattes.cnpq.br/1519648219507868 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/0273230600642926 |
dc.contributor.author.fl_str_mv |
Heck, Suélen Osório |
contributor_str_mv |
Zeni, Gilson Rogério Rocha, Juliana Trevisan da Morsch, Vera Maria Melchiors |
dc.subject.por.fl_str_mv |
Compostos orgânicos de selênio Dislipidemia Hepatotoxicidade Triton WR – 1339 Ratos |
topic |
Compostos orgânicos de selênio Dislipidemia Hepatotoxicidade Triton WR – 1339 Ratos Organoselenium compound Dyslipidemia Hepatotoxicity Rats CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA |
dc.subject.eng.fl_str_mv |
Organoselenium compound Dyslipidemia Hepatotoxicity Rats |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA |
description |
Hyperlipidemia can be manifested by elevation in total cholesterol (TC), triglycerides (TGs) and low density lipoprotein (LDL), as well as by reduction in high density lipoprotein (HDL) levels. Hyperlipidemia is associated with oxidative stress and endothelial damage followed by atheroma and cardiovascular disease (CVDs) development. Moreover, high lipid levels are related with non alcoholic fatty liver disease (NAFLD). In view of the limitations and side effects of current drugs to treat hyperlipidemia, it becomes interesting to search for new drugs with hypolipidemic and hepatoprotective effects. The purpose of this study was to investigate the possible hepatoprotective and antihyperlipidemic effects of 4,4'-dichlorodiphenyl diselenide [(p-ClPhSe)2] in a hyperlipidemia model induced by Triton WR-1339 in rats. Biochemical analyses and hepatic oxidative stress parameters were evaluated in rats exposed to triton WR-1339 (400 mg/kg; i.p.) and treated with (p-ClPhSe)2 (10 mg/kg; i.g.) for seven days. After triton WR-1339 injection and fasting for 18 hours, the animals were exposed to the activity monitor for evaluation of exploratory and locomotor activity. Subsequently, blood was collected for determining biochemical parameters. The levels of TC, TGs, non-HDL-cholesterol (non-HDL), coronary risk index (CRI), reactive oxygen species, alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activities were significantly increased in triton treated rats. (p-ClPhSe)2 treatment resulted in a significant decrease in plasma lipid levels and was effective in normalizing the enzyme activities. (p-ClPhSe)2 did not protect against the increase of ROS levels, but increased NPHS levels in triton treated animals. (p-ClPhSe)2 could have potential in hyperlipidemia treatment as well as to reduce liver damage caused by this disorder. |
publishDate |
2016 |
dc.date.issued.fl_str_mv |
2016-02-29 |
dc.date.accessioned.fl_str_mv |
2019-07-05T21:46:57Z |
dc.date.available.fl_str_mv |
2019-07-05T21:46:57Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
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masterThesis |
status_str |
publishedVersion |
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http://repositorio.ufsm.br/handle/1/17339 |
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http://repositorio.ufsm.br/handle/1/17339 |
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por |
language |
por |
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200800000002 |
dc.relation.confidence.fl_str_mv |
600 |
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Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Universidade Federal de Santa Maria Centro de Ciências Naturais e Exatas |
dc.publisher.program.fl_str_mv |
Programa de Pós-Graduação em Ciências Biológicas: Bioquímica Toxicológica |
dc.publisher.initials.fl_str_mv |
UFSM |
dc.publisher.country.fl_str_mv |
Brasil |
dc.publisher.department.fl_str_mv |
Bioquímica |
publisher.none.fl_str_mv |
Universidade Federal de Santa Maria Centro de Ciências Naturais e Exatas |
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