Aceturato de diminazeno lipossomal no tratamento da infecção por Trypanosoma evansi: testes in vitro e in vivo
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Tipo de documento: | Tese |
Idioma: | por |
Título da fonte: | Manancial - Repositório Digital da UFSM |
Texto Completo: | http://repositorio.ufsm.br/handle/1/4099 |
Resumo: | The aim of this study was to develop and to evaluate the therapeutic efficacy of liposomes diminazene aceturate and in vitro and by using mice experimentally infected with Trypanosoma evansi. In vitro tests were performed in culture medium at concentrations of 0.25, 0.5, 1, 2 and 3 mg/ml of diminazene aceturate convetional (CDMZ) and liposomal (L-DMZ). A total of 114 rats (Rattus norvegicus) were used of in vivo test. These rats were divided into 6 groups (A, B, C, D, E and F). Group A served as a negative control (uninfected and untreated). Rats in Group B served as a positive control and were infected with T. evansi. Animals in Group C were infected and treated with L-DMZ (single dose, 3.5 mg/kg-1), Group D was composed with infected and treated with C-DMZ animals (single dose, 3.5 mg/kg-1), Group E infected treated with L-DMZ animals for 5 consecutive days (3.5 mg/kg-1/dia) and Group F infected treated with C-DMZ animals for 5 consecutive days (3.5 mg/kg-1/dia). In vitro, a dose-dependent trypanocidal effect of L-DMZ was observed against the parasite. In vivo, the efficacy of L-DMZ and C-DMZ in different therapeutic protocols was similar. The analysis of biochemical and histological parameters on the 7th and 40th post-treatment revealed alterations in liver and kidney enzymes, and histological alterations in the structure of organs, especially in the animals treated with L-DMZ at 5 consecutive days. The results of this study showed that liposomal formulations may be a new alternative for the treatment of tripanosomoses, but future research could be undertaken to improve the conduction of liposomes and direction for greater efficiency. |
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2017-05-302017-05-302014-07-02OLIVEIRA, Camila Belmonte. Liposomal diminazene aceturate of the treatment of infection Trypanosoma evansi: in vitro and in vivo. 2014. 100 f. Tese (Doutorado em Medicina Veterinária) - Universidade Federal de Santa Maria, Santa Maria, 2014.http://repositorio.ufsm.br/handle/1/4099The aim of this study was to develop and to evaluate the therapeutic efficacy of liposomes diminazene aceturate and in vitro and by using mice experimentally infected with Trypanosoma evansi. In vitro tests were performed in culture medium at concentrations of 0.25, 0.5, 1, 2 and 3 mg/ml of diminazene aceturate convetional (CDMZ) and liposomal (L-DMZ). A total of 114 rats (Rattus norvegicus) were used of in vivo test. These rats were divided into 6 groups (A, B, C, D, E and F). Group A served as a negative control (uninfected and untreated). Rats in Group B served as a positive control and were infected with T. evansi. Animals in Group C were infected and treated with L-DMZ (single dose, 3.5 mg/kg-1), Group D was composed with infected and treated with C-DMZ animals (single dose, 3.5 mg/kg-1), Group E infected treated with L-DMZ animals for 5 consecutive days (3.5 mg/kg-1/dia) and Group F infected treated with C-DMZ animals for 5 consecutive days (3.5 mg/kg-1/dia). In vitro, a dose-dependent trypanocidal effect of L-DMZ was observed against the parasite. In vivo, the efficacy of L-DMZ and C-DMZ in different therapeutic protocols was similar. The analysis of biochemical and histological parameters on the 7th and 40th post-treatment revealed alterations in liver and kidney enzymes, and histological alterations in the structure of organs, especially in the animals treated with L-DMZ at 5 consecutive days. The results of this study showed that liposomal formulations may be a new alternative for the treatment of tripanosomoses, but future research could be undertaken to improve the conduction of liposomes and direction for greater efficiency.Este estudo teve como objetivo desenvolver e testar lipossomas de aceturato de diminazeno em testes in vitro e in vivo visando o controle de Trypanosoma evansi. O teste in vitro foi realizado em meio de cultura nas concentrações de 0,25, 0,5, 1, 2 e 3 μg/mL de aceturato de diminazeno convencional (C-DMZ) e lipossomal (L-DMZ). Para os testes in vivo foram utilizados 114 ratos (Rattus norvegicus) divididos em seis grupos (A, B, C, D, E e F) em dois experimentos, um para avaliar a eficácia e outro para analisar os parâmetros bioquímicos e histológicos. O grupo A foi utilizado como controle (animais sadios), B (animais infectados e não tratados), C (animais infectados e tratados com aceturato de diminazeno lipossomal com dose única 3,5 mg/kg-1), D (animais infectados e tratados com aceturato de diminazeno convencional com dose única 3,5 mg/kg-1), E (animais infectados e tratados com aceturato lipossomal por 5 dias consecutivos com a dose de 3,5 mg/kg-1/dia) e grupo F (animais e infectados tratados com aceturato convencional por 5 dias consecutivos com a dose de 3,5 mg/kg-1/dia). O teste in vitro com o lipossoma de aceturato de diminazeno mostrou uma maior mortalidade dose-dependente do T. evansi em meio de cultura se comparado ao medicamento comercial e os parasitos morreram mais rapidamente que nos grupos de aceturato de diminazeno convencional e controle. Nos resultados dos testes in vivo, a eficácia do aceturato de diminazeno lipossomal e convencional nos diferentes protocolos terapêuticos foram similares. A análise dos parâmetros bioquímicos e histológicos realizados no 7º e 40º pós-tratamento revelaram alterações nas enzimas hepáticas e renais, além de modificações na estrutura dos órgãos, principalmente nos animais tratados com lipossomas na maior dosagem. Os resultados obtidos neste estudo demonstraram que as formulações lipossomais podem ser uma nova alternativa para o tratamento das tripanossomoses. Futuras pesquisas poderiam ser realizadas para melhorar o carreamento e a direção dos lipossomas para uma maior eficácia.Coordenação de Aperfeiçoamento de Pessoal de Nível Superiorapplication/pdfporUniversidade Federal de Santa MariaPrograma de Pós-Graduação em Medicina VeterináriaUFSMBRMedicina VeterináriaNanoestruturasTripanossomosesDiamidinasNanostructuresTripanossomosesDiamidinesCNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIAAceturato de diminazeno lipossomal no tratamento da infecção por Trypanosoma evansi: testes in vitro e in vivoLiposomal diminazene aceturate of the treatment of infection Trypanosoma evansi: in vitro and in vivoinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisMonteiro, Silvia Gonzalezhttp://lattes.cnpq.br/3762606653182779Leal, Marta Lizandra do Rêgohttp://lattes.cnpq.br/6859797230596402Sangioni, Luis Antoniohttp://lattes.cnpq.br/8056805667740451Santos, Roberto Christ Viannahttp://lattes.cnpq.br/9176719594431835Krause, Luciana Maria Fontanarihttp://lattes.cnpq.br/9844890896121847http://lattes.cnpq.br/1309395007961788Oliveira, Camila Belmonte500500000007400300300300300300300f658c142-7522-4cd5-a133-f2479652bf3187419040-500d-4516-8802-ee0b3a74fcd033a7a303-c4ab-4c50-a2ba-cd610e4bce1547b3fb7c-79e3-4fce-8565-4d0c754e0310763e5001-67be-4e8d-8d7e-d6c32dda561ca45db657-fbef-4efc-a2e6-1b1ebab5c203info:eu-repo/semantics/openAccessreponame:Manancial - Repositório Digital da UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSMORIGINALOLIVEIRA, CAMILA BELMONTE.pdfapplication/pdf2790763http://repositorio.ufsm.br/bitstream/1/4099/1/OLIVEIRA%2c%20CAMILA%20BELMONTE.pdf2a97fc8f0c74f8e206640b7ccb124205MD51TEXTOLIVEIRA, CAMILA BELMONTE.pdf.txtOLIVEIRA, CAMILA BELMONTE.pdf.txtExtracted texttext/plain160672http://repositorio.ufsm.br/bitstream/1/4099/2/OLIVEIRA%2c%20CAMILA%20BELMONTE.pdf.txtc67750967acde557d094f866c9aa7b97MD52THUMBNAILOLIVEIRA, CAMILA BELMONTE.pdf.jpgOLIVEIRA, CAMILA BELMONTE.pdf.jpgIM Thumbnailimage/jpeg4832http://repositorio.ufsm.br/bitstream/1/4099/3/OLIVEIRA%2c%20CAMILA%20BELMONTE.pdf.jpgbfd5da4a148194450e5767c67866d8d4MD531/40992022-04-05 16:27:01.487oai:repositorio.ufsm.br:1/4099Repositório Institucionalhttp://repositorio.ufsm.br/PUBhttp://repositorio.ufsm.br/oai/requestopendoar:39132022-04-05T19:27:01Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM)false |
dc.title.por.fl_str_mv |
Aceturato de diminazeno lipossomal no tratamento da infecção por Trypanosoma evansi: testes in vitro e in vivo |
dc.title.alternative.eng.fl_str_mv |
Liposomal diminazene aceturate of the treatment of infection Trypanosoma evansi: in vitro and in vivo |
title |
Aceturato de diminazeno lipossomal no tratamento da infecção por Trypanosoma evansi: testes in vitro e in vivo |
spellingShingle |
Aceturato de diminazeno lipossomal no tratamento da infecção por Trypanosoma evansi: testes in vitro e in vivo Oliveira, Camila Belmonte Nanoestruturas Tripanossomoses Diamidinas Nanostructures Tripanossomoses Diamidines CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
title_short |
Aceturato de diminazeno lipossomal no tratamento da infecção por Trypanosoma evansi: testes in vitro e in vivo |
title_full |
Aceturato de diminazeno lipossomal no tratamento da infecção por Trypanosoma evansi: testes in vitro e in vivo |
title_fullStr |
Aceturato de diminazeno lipossomal no tratamento da infecção por Trypanosoma evansi: testes in vitro e in vivo |
title_full_unstemmed |
Aceturato de diminazeno lipossomal no tratamento da infecção por Trypanosoma evansi: testes in vitro e in vivo |
title_sort |
Aceturato de diminazeno lipossomal no tratamento da infecção por Trypanosoma evansi: testes in vitro e in vivo |
author |
Oliveira, Camila Belmonte |
author_facet |
Oliveira, Camila Belmonte |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Monteiro, Silvia Gonzalez |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/3762606653182779 |
dc.contributor.referee1.fl_str_mv |
Leal, Marta Lizandra do Rêgo |
dc.contributor.referee1Lattes.fl_str_mv |
http://lattes.cnpq.br/6859797230596402 |
dc.contributor.referee2.fl_str_mv |
Sangioni, Luis Antonio |
dc.contributor.referee2Lattes.fl_str_mv |
http://lattes.cnpq.br/8056805667740451 |
dc.contributor.referee3.fl_str_mv |
Santos, Roberto Christ Vianna |
dc.contributor.referee3Lattes.fl_str_mv |
http://lattes.cnpq.br/9176719594431835 |
dc.contributor.referee4.fl_str_mv |
Krause, Luciana Maria Fontanari |
dc.contributor.referee4Lattes.fl_str_mv |
http://lattes.cnpq.br/9844890896121847 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/1309395007961788 |
dc.contributor.author.fl_str_mv |
Oliveira, Camila Belmonte |
contributor_str_mv |
Monteiro, Silvia Gonzalez Leal, Marta Lizandra do Rêgo Sangioni, Luis Antonio Santos, Roberto Christ Vianna Krause, Luciana Maria Fontanari |
dc.subject.por.fl_str_mv |
Nanoestruturas Tripanossomoses Diamidinas |
topic |
Nanoestruturas Tripanossomoses Diamidinas Nanostructures Tripanossomoses Diamidines CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
dc.subject.eng.fl_str_mv |
Nanostructures Tripanossomoses Diamidines |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
description |
The aim of this study was to develop and to evaluate the therapeutic efficacy of liposomes diminazene aceturate and in vitro and by using mice experimentally infected with Trypanosoma evansi. In vitro tests were performed in culture medium at concentrations of 0.25, 0.5, 1, 2 and 3 mg/ml of diminazene aceturate convetional (CDMZ) and liposomal (L-DMZ). A total of 114 rats (Rattus norvegicus) were used of in vivo test. These rats were divided into 6 groups (A, B, C, D, E and F). Group A served as a negative control (uninfected and untreated). Rats in Group B served as a positive control and were infected with T. evansi. Animals in Group C were infected and treated with L-DMZ (single dose, 3.5 mg/kg-1), Group D was composed with infected and treated with C-DMZ animals (single dose, 3.5 mg/kg-1), Group E infected treated with L-DMZ animals for 5 consecutive days (3.5 mg/kg-1/dia) and Group F infected treated with C-DMZ animals for 5 consecutive days (3.5 mg/kg-1/dia). In vitro, a dose-dependent trypanocidal effect of L-DMZ was observed against the parasite. In vivo, the efficacy of L-DMZ and C-DMZ in different therapeutic protocols was similar. The analysis of biochemical and histological parameters on the 7th and 40th post-treatment revealed alterations in liver and kidney enzymes, and histological alterations in the structure of organs, especially in the animals treated with L-DMZ at 5 consecutive days. The results of this study showed that liposomal formulations may be a new alternative for the treatment of tripanosomoses, but future research could be undertaken to improve the conduction of liposomes and direction for greater efficiency. |
publishDate |
2014 |
dc.date.issued.fl_str_mv |
2014-07-02 |
dc.date.accessioned.fl_str_mv |
2017-05-30 |
dc.date.available.fl_str_mv |
2017-05-30 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/doctoralThesis |
format |
doctoralThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
OLIVEIRA, Camila Belmonte. Liposomal diminazene aceturate of the treatment of infection Trypanosoma evansi: in vitro and in vivo. 2014. 100 f. Tese (Doutorado em Medicina Veterinária) - Universidade Federal de Santa Maria, Santa Maria, 2014. |
dc.identifier.uri.fl_str_mv |
http://repositorio.ufsm.br/handle/1/4099 |
identifier_str_mv |
OLIVEIRA, Camila Belmonte. Liposomal diminazene aceturate of the treatment of infection Trypanosoma evansi: in vitro and in vivo. 2014. 100 f. Tese (Doutorado em Medicina Veterinária) - Universidade Federal de Santa Maria, Santa Maria, 2014. |
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Medicina Veterinária |
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