Leishmania infantum in the reproductive organs of dogs
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Ciência Rural |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782021001000501 |
Resumo: | ABSTRACT: Leishmania infantum causes canine leishmaniasis. Using parasitological and molecular analyses, we identified L. infantum in the reproductive organs of male and female dogs. Using histochemistry, immunohistochemistry, and PCR, we examined tissue samples from the reproductive organs of 8 male dogs and 16 female dogs diagnosed with leishmaniasis. Despite the absence of macroscopic or microscopic lesions in these organs, we observed L. infantum amastigotes in tissue samples from the testis and the uterus. PCR and sequencing of these tissues revealed sequences that matched 100% with L. infantum DNA available at GenBank. The presence of L. infantum amastigotes and DNA in testicular and uterine tissue samples suggested that these organs can harbor the parasite without associated macroscopic or microscopic lesions, and this can be especially important in the vertical and venereal transmission of leishmaniasis in dogs. |
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Ciência rural (Online) |
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Leishmania infantum in the reproductive organs of dogshistochemicalimmunohistochemicalleishmaniasistestisuterusABSTRACT: Leishmania infantum causes canine leishmaniasis. Using parasitological and molecular analyses, we identified L. infantum in the reproductive organs of male and female dogs. Using histochemistry, immunohistochemistry, and PCR, we examined tissue samples from the reproductive organs of 8 male dogs and 16 female dogs diagnosed with leishmaniasis. Despite the absence of macroscopic or microscopic lesions in these organs, we observed L. infantum amastigotes in tissue samples from the testis and the uterus. PCR and sequencing of these tissues revealed sequences that matched 100% with L. infantum DNA available at GenBank. The presence of L. infantum amastigotes and DNA in testicular and uterine tissue samples suggested that these organs can harbor the parasite without associated macroscopic or microscopic lesions, and this can be especially important in the vertical and venereal transmission of leishmaniasis in dogs.Universidade Federal de Santa Maria2021-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782021001000501Ciência Rural v.51 n.10 2021reponame:Ciência Ruralinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSM10.1590/0103-8478cr20200825info:eu-repo/semantics/openAccessSilva,Diogo Tiago daAlves,Maria LuanaSpada,Julio Cesar PereiraLeonel,João Augusto FrancoBenassi,Julia CristinaStarke-Buzetti,Wilma AparecidaFerreira,Helena LageKeid,Lara BorgesSoares,Rodrigo MartinsOliveira,Trícia Maria Ferreira de Sousaeng2021-06-21T00:00:00ZRevista |
dc.title.none.fl_str_mv |
Leishmania infantum in the reproductive organs of dogs |
title |
Leishmania infantum in the reproductive organs of dogs |
spellingShingle |
Leishmania infantum in the reproductive organs of dogs Silva,Diogo Tiago da histochemical immunohistochemical leishmaniasis testis uterus |
title_short |
Leishmania infantum in the reproductive organs of dogs |
title_full |
Leishmania infantum in the reproductive organs of dogs |
title_fullStr |
Leishmania infantum in the reproductive organs of dogs |
title_full_unstemmed |
Leishmania infantum in the reproductive organs of dogs |
title_sort |
Leishmania infantum in the reproductive organs of dogs |
author |
Silva,Diogo Tiago da |
author_facet |
Silva,Diogo Tiago da Alves,Maria Luana Spada,Julio Cesar Pereira Leonel,João Augusto Franco Benassi,Julia Cristina Starke-Buzetti,Wilma Aparecida Ferreira,Helena Lage Keid,Lara Borges Soares,Rodrigo Martins Oliveira,Trícia Maria Ferreira de Sousa |
author_role |
author |
author2 |
Alves,Maria Luana Spada,Julio Cesar Pereira Leonel,João Augusto Franco Benassi,Julia Cristina Starke-Buzetti,Wilma Aparecida Ferreira,Helena Lage Keid,Lara Borges Soares,Rodrigo Martins Oliveira,Trícia Maria Ferreira de Sousa |
author2_role |
author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Silva,Diogo Tiago da Alves,Maria Luana Spada,Julio Cesar Pereira Leonel,João Augusto Franco Benassi,Julia Cristina Starke-Buzetti,Wilma Aparecida Ferreira,Helena Lage Keid,Lara Borges Soares,Rodrigo Martins Oliveira,Trícia Maria Ferreira de Sousa |
dc.subject.por.fl_str_mv |
histochemical immunohistochemical leishmaniasis testis uterus |
topic |
histochemical immunohistochemical leishmaniasis testis uterus |
description |
ABSTRACT: Leishmania infantum causes canine leishmaniasis. Using parasitological and molecular analyses, we identified L. infantum in the reproductive organs of male and female dogs. Using histochemistry, immunohistochemistry, and PCR, we examined tissue samples from the reproductive organs of 8 male dogs and 16 female dogs diagnosed with leishmaniasis. Despite the absence of macroscopic or microscopic lesions in these organs, we observed L. infantum amastigotes in tissue samples from the testis and the uterus. PCR and sequencing of these tissues revealed sequences that matched 100% with L. infantum DNA available at GenBank. The presence of L. infantum amastigotes and DNA in testicular and uterine tissue samples suggested that these organs can harbor the parasite without associated macroscopic or microscopic lesions, and this can be especially important in the vertical and venereal transmission of leishmaniasis in dogs. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782021001000501 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782021001000501 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/0103-8478cr20200825 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Universidade Federal de Santa Maria |
publisher.none.fl_str_mv |
Universidade Federal de Santa Maria |
dc.source.none.fl_str_mv |
Ciência Rural v.51 n.10 2021 reponame:Ciência Rural instname:Universidade Federal de Santa Maria (UFSM) instacron:UFSM |
instname_str |
Universidade Federal de Santa Maria (UFSM) |
instacron_str |
UFSM |
institution |
UFSM |
reponame_str |
Ciência Rural |
collection |
Ciência Rural |
repository.name.fl_str_mv |
|
repository.mail.fl_str_mv |
|
_version_ |
1749140556148113408 |