Alternatives for obtaining a continuous cell line from Apis mellifera
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Ciência Rural |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782021001200401 |
Resumo: | ABSTRACT: In worldwide there are reports of a significant decrease in colonies of the species Apis mellifera, caused by several factors, including viral infections. In order to study and diagnose illnesses caused by viruses, in vitro cell culture is used as a valuable tool. Yet, there are still no immortalized cell lines of honey bee Apis mellifera. Primary cell cultures are promising for this purpose and can supply the lack of continuous strains, but their establishment is difficult and laborious, which often makes them unfeasible for many research centers. Through the use of cell immortalization techniques, it is possible to develop continuous cell lines and thus benefit, in different ways, research related to different species of bees. The choice of technique is challenging, since in addition to the ability to remain viable for countless passages, cells must keep the genotype and phenotype similar or identical to the original tissue. This review intends to present methodologies that can be used to immortalize Apis mellifera cells, aiming to establish a cell line. The genotypic and phenotypic implications of each technique are evaluated, and the purpose of the cell line to be developed. |
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Alternatives for obtaining a continuous cell line from Apis melliferaimmortalizationcell culturecell linehoneybeeABSTRACT: In worldwide there are reports of a significant decrease in colonies of the species Apis mellifera, caused by several factors, including viral infections. In order to study and diagnose illnesses caused by viruses, in vitro cell culture is used as a valuable tool. Yet, there are still no immortalized cell lines of honey bee Apis mellifera. Primary cell cultures are promising for this purpose and can supply the lack of continuous strains, but their establishment is difficult and laborious, which often makes them unfeasible for many research centers. Through the use of cell immortalization techniques, it is possible to develop continuous cell lines and thus benefit, in different ways, research related to different species of bees. The choice of technique is challenging, since in addition to the ability to remain viable for countless passages, cells must keep the genotype and phenotype similar or identical to the original tissue. This review intends to present methodologies that can be used to immortalize Apis mellifera cells, aiming to establish a cell line. The genotypic and phenotypic implications of each technique are evaluated, and the purpose of the cell line to be developed.Universidade Federal de Santa Maria2021-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782021001200401Ciência Rural v.51 n.12 2021reponame:Ciência Ruralinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSM10.1590/0103-8478cr20201111info:eu-repo/semantics/openAccessFrühauf,Matheus IuriBarcelos,Lariane da SilvaBotton,Nadálin YandraPeter,Cristina MendesHübner,Silvia de OliveiraLima,Marcelo deVargas,Gilberto D´ÁvilaFischer,Gefersoneng2021-07-22T00:00:00ZRevista |
dc.title.none.fl_str_mv |
Alternatives for obtaining a continuous cell line from Apis mellifera |
title |
Alternatives for obtaining a continuous cell line from Apis mellifera |
spellingShingle |
Alternatives for obtaining a continuous cell line from Apis mellifera Frühauf,Matheus Iuri immortalization cell culture cell line honeybee |
title_short |
Alternatives for obtaining a continuous cell line from Apis mellifera |
title_full |
Alternatives for obtaining a continuous cell line from Apis mellifera |
title_fullStr |
Alternatives for obtaining a continuous cell line from Apis mellifera |
title_full_unstemmed |
Alternatives for obtaining a continuous cell line from Apis mellifera |
title_sort |
Alternatives for obtaining a continuous cell line from Apis mellifera |
author |
Frühauf,Matheus Iuri |
author_facet |
Frühauf,Matheus Iuri Barcelos,Lariane da Silva Botton,Nadálin Yandra Peter,Cristina Mendes Hübner,Silvia de Oliveira Lima,Marcelo de Vargas,Gilberto D´Ávila Fischer,Geferson |
author_role |
author |
author2 |
Barcelos,Lariane da Silva Botton,Nadálin Yandra Peter,Cristina Mendes Hübner,Silvia de Oliveira Lima,Marcelo de Vargas,Gilberto D´Ávila Fischer,Geferson |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Frühauf,Matheus Iuri Barcelos,Lariane da Silva Botton,Nadálin Yandra Peter,Cristina Mendes Hübner,Silvia de Oliveira Lima,Marcelo de Vargas,Gilberto D´Ávila Fischer,Geferson |
dc.subject.por.fl_str_mv |
immortalization cell culture cell line honeybee |
topic |
immortalization cell culture cell line honeybee |
description |
ABSTRACT: In worldwide there are reports of a significant decrease in colonies of the species Apis mellifera, caused by several factors, including viral infections. In order to study and diagnose illnesses caused by viruses, in vitro cell culture is used as a valuable tool. Yet, there are still no immortalized cell lines of honey bee Apis mellifera. Primary cell cultures are promising for this purpose and can supply the lack of continuous strains, but their establishment is difficult and laborious, which often makes them unfeasible for many research centers. Through the use of cell immortalization techniques, it is possible to develop continuous cell lines and thus benefit, in different ways, research related to different species of bees. The choice of technique is challenging, since in addition to the ability to remain viable for countless passages, cells must keep the genotype and phenotype similar or identical to the original tissue. This review intends to present methodologies that can be used to immortalize Apis mellifera cells, aiming to establish a cell line. The genotypic and phenotypic implications of each technique are evaluated, and the purpose of the cell line to be developed. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782021001200401 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782021001200401 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/0103-8478cr20201111 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Universidade Federal de Santa Maria |
publisher.none.fl_str_mv |
Universidade Federal de Santa Maria |
dc.source.none.fl_str_mv |
Ciência Rural v.51 n.12 2021 reponame:Ciência Rural instname:Universidade Federal de Santa Maria (UFSM) instacron:UFSM |
instname_str |
Universidade Federal de Santa Maria (UFSM) |
instacron_str |
UFSM |
institution |
UFSM |
reponame_str |
Ciência Rural |
collection |
Ciência Rural |
repository.name.fl_str_mv |
|
repository.mail.fl_str_mv |
|
_version_ |
1749140556466880512 |