Sistema colinérgico e peroxidação lipídica de ratos tratados com sulfato de vincristina e decanoato de nandrolona
Autor(a) principal: | |
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Data de Publicação: | 2008 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Biblioteca Digital de Teses e Dissertações do UFSM |
Texto Completo: | http://repositorio.ufsm.br/handle/1/10011 |
Resumo: | Vincristine sulphate is antitumor agent widely used in small animal clinical oncology; therefore it can cause a number of adverse effects including marrow and neuronal cytotoxicity. Nandrolone decanoate, an anabolic-androgenic steroid (AAS), has been used in association with vincristine in order to ease effects such as moderate myelosuppression. The present dissertation presents data related to the isolated or associated employment of vincristine sulphate and nandrolone decanoate, as well as their effects on the cholinergic system and oxidative profile of Wistar rats. The animals were submitted to four different doses of AAS for three weeks and its action on acetylcholinesterase (AChE) activity in four different structures of brain tissue (cerebellum, hippocampus, striatum and cerebral cortex) was studied. The second experiment shows the effects of vincristine and/or nandrolone decanoate in the brain (same parts studied previously) and blood through measurement of brain and red blood cell AChE (RBC-AChE) enzyme activity as well as brain and blood serum lipid peroxidation of rats treated for two weeks. Results show that the two highest doses used in the first experiment increased enzyme activity, suggesting interference in the cholinergical system of the striatum and cerebellum. The results obtained in the latter experiment demonstrate that the isolated use of this AAS and its association with vincristine sulphate altered brain and RBC-AChE action, both in a stimulatory and inhibitory fashion. Lipid peroxidation, in the brain and blood, increased due to the isolated use of both vincristine and nandrolone decanoate, as well as to their associated use at the highest dose of ester used. Furthermore, the data show that the association between the therapeutic dose of nandrolone decanoate and vincristine is capable of neutralizing the free radical production induced by their isolated use in brain and blood serum. Serum RBC-AChE activity and the oxidative profile presented in this study are similar to those exhibited for brain tissue. Based on these data, it can be concluded that nandrolone decanoate is capable of interfering in AChE activity, affecting the cholinergic system, which could cause an alteration of its neurotransmitter, as well as a low or high stimulation of post-synaptic receptors. Therefore, the use of the therapeutic dose of AAS studied here in association with vincristine has been shown to be beneficial, as it could protect the organism from damaging processes caused by the production of free radicals. |
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2008-03-272008-03-272008-02-22MARTINS, Danieli Brolo. Evaluation of cholinergic system and lipid peroxidation of rats treated with vincristine sulphate and nandrolone decanoate. 2008. 86 f. Dissertação (Mestrado em Medicina Veterinária) - Universidade Federal de Santa Maria, Santa Maria, 2008.http://repositorio.ufsm.br/handle/1/10011Vincristine sulphate is antitumor agent widely used in small animal clinical oncology; therefore it can cause a number of adverse effects including marrow and neuronal cytotoxicity. Nandrolone decanoate, an anabolic-androgenic steroid (AAS), has been used in association with vincristine in order to ease effects such as moderate myelosuppression. The present dissertation presents data related to the isolated or associated employment of vincristine sulphate and nandrolone decanoate, as well as their effects on the cholinergic system and oxidative profile of Wistar rats. The animals were submitted to four different doses of AAS for three weeks and its action on acetylcholinesterase (AChE) activity in four different structures of brain tissue (cerebellum, hippocampus, striatum and cerebral cortex) was studied. The second experiment shows the effects of vincristine and/or nandrolone decanoate in the brain (same parts studied previously) and blood through measurement of brain and red blood cell AChE (RBC-AChE) enzyme activity as well as brain and blood serum lipid peroxidation of rats treated for two weeks. Results show that the two highest doses used in the first experiment increased enzyme activity, suggesting interference in the cholinergical system of the striatum and cerebellum. The results obtained in the latter experiment demonstrate that the isolated use of this AAS and its association with vincristine sulphate altered brain and RBC-AChE action, both in a stimulatory and inhibitory fashion. Lipid peroxidation, in the brain and blood, increased due to the isolated use of both vincristine and nandrolone decanoate, as well as to their associated use at the highest dose of ester used. Furthermore, the data show that the association between the therapeutic dose of nandrolone decanoate and vincristine is capable of neutralizing the free radical production induced by their isolated use in brain and blood serum. Serum RBC-AChE activity and the oxidative profile presented in this study are similar to those exhibited for brain tissue. Based on these data, it can be concluded that nandrolone decanoate is capable of interfering in AChE activity, affecting the cholinergic system, which could cause an alteration of its neurotransmitter, as well as a low or high stimulation of post-synaptic receptors. Therefore, the use of the therapeutic dose of AAS studied here in association with vincristine has been shown to be beneficial, as it could protect the organism from damaging processes caused by the production of free radicals.O sulfato de vincristina é um agente anti-tumoral bastante usado na oncologia clínica de pequenos animais, porém seus efeitos colaterais incluem citotoxicidade medular e neuronal. O decanoato de nandrolona, um esteróide anabólico androgênico (EAA), tem sido usado em associação a este medicamento para amenizar alguns de seus efeitos, como a mielossupressão moderada. Esta dissertação apresenta dados referentes ao uso isolado ou associado do sulfato de vincristina e do decanoato de nandrolona, seus efeitos no sistema colinérgico e no perfil oxidativo de ratos Wistar. Primeiramente, verificou-se quatro diferentes doses do EAA, por três semanas, e sua ação sobre a atividade da acetilcolinesterase (AChE) em quatro partes do tecido cerebral (cerebelo, estriado, hipocampo e córtex cerebral). O segundo experimento apresenta grupos tratados durante duas semanas com sulfato de vincristina e/ou decanoato de nandrolona e as ações destes no cérebro (mesmas partes pesquisadas anteriormente) e no sangue, através da mensuração da atividade enzimática da AChE cerebral e eritrocitária (RBC-AChE) e peroxidação lipídica cerebral e sérica. As duas doses mais altas utilizadas no primeiro trabalho aumentam a atividade da enzima, sugerindo que haja interferência no sistema colinérgico, no estriado e no cerebelo. Os resultados obtidos, no estudo posterior, demonstram que o uso isolado deste EAA e suas associações com o sulfato de vincristina alteram a ação da AChE cerebral e RBC-AChE, tanto de forma estimulatória quanto inibitória. A peroxidação lipídica, cerebral e sangüínea, aumenta devido ao uso isolado da vincristina e do decanoato de nandrolona, e na associação do quimioterápico com a dose mais alta usada do éster. A dose terapêutica do decanoato de nandrolona e a vincristina utilizadas são capazes de neutralizar a produção de radicais livres tanto no cérebro quanto no soro sangüíneo. A atividade da RBC-AChE e o valor do perfil oxidativo do soro apresentados nesta pesquisa são similares àqueles exibidos pelo tecido cerebral. Diante destes dados, pode-se concluir que o decanoato de nandrolona é capaz de influenciar a atividade da AChE, afetando o sistema colinérgico, o que poderia ocasionar em uma ação alterada do seu neurotransmissor, além de uma baixa ou alta estimulação dos receptores póssinápticos. Entretanto, o uso da dose terapêutica estudada deste EAA associada à vincristina mostra-se benéfico, pois poderia proteger o organismo de processos prejudiciais relacionados a produção de radicais livres.Coordenação de Aperfeiçoamento de Pessoal de Nível Superiorapplication/pdfporUniversidade Federal de Santa MariaPrograma de Pós-Graduação em Medicina VeterináriaUFSMBRMedicina VeterináriaEsteróide anabólico androgênicoQuimioterápicoAcetilcolinesterasePeroxidação lipídicaRatosAnabolic androgenic steroidChemotherapeuticAcetylcholinesteraseLipid peroxidationRatsCNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIASistema colinérgico e peroxidação lipídica de ratos tratados com sulfato de vincristina e decanoato de nandrolonaEvaluation of cholinergic system and lipid peroxidation of rats treated with vincristine sulphate and nandrolone decanoateinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisLopes, Sonia Terezinha dos Anjoshttp://lattes.cnpq.br/8059723754130756Schetinger, Maria Rosa ChitolinaLago, Lissandra Dalhttp://lattes.cnpq.br/7448358134461750http://lattes.cnpq.br/2159963349521553Martins, Danieli Brolo5005000000074005005005005000e22bc1c-35eb-49dd-915f-0d5c7bca25b7bac5777c-9616-4b97-acc2-0c48d0d52569cba4a833-b1c5-4608-8453-7a46f671faf1d24ae8c7-9b57-495d-95a9-a11c8afb4d79info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações do UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSMORIGINALDANIELI MARTINS.pdfapplication/pdf1304991http://repositorio.ufsm.br/bitstream/1/10011/1/DANIELI%20MARTINS.pdf1424fbe79f54f747c02d6b0f1c1046c4MD51TEXTDANIELI MARTINS.pdf.txtDANIELI MARTINS.pdf.txtExtracted texttext/plain129725http://repositorio.ufsm.br/bitstream/1/10011/2/DANIELI%20MARTINS.pdf.txt2a53d80033652e6532999bb17f8fb1c9MD52THUMBNAILDANIELI MARTINS.pdf.jpgDANIELI MARTINS.pdf.jpgIM Thumbnailimage/jpeg5221http://repositorio.ufsm.br/bitstream/1/10011/3/DANIELI%20MARTINS.pdf.jpga08a8a5ddabb84093ec5486873c29485MD531/100112022-08-11 15:41:37.809oai:repositorio.ufsm.br:1/10011Biblioteca Digital de Teses e Dissertaçõeshttps://repositorio.ufsm.br/ONGhttps://repositorio.ufsm.br/oai/requestatendimento.sib@ufsm.br||tedebc@gmail.comopendoar:2022-08-11T18:41:37Biblioteca Digital de Teses e Dissertações do UFSM - Universidade Federal de Santa Maria (UFSM)false |
dc.title.por.fl_str_mv |
Sistema colinérgico e peroxidação lipídica de ratos tratados com sulfato de vincristina e decanoato de nandrolona |
dc.title.alternative.eng.fl_str_mv |
Evaluation of cholinergic system and lipid peroxidation of rats treated with vincristine sulphate and nandrolone decanoate |
title |
Sistema colinérgico e peroxidação lipídica de ratos tratados com sulfato de vincristina e decanoato de nandrolona |
spellingShingle |
Sistema colinérgico e peroxidação lipídica de ratos tratados com sulfato de vincristina e decanoato de nandrolona Martins, Danieli Brolo Esteróide anabólico androgênico Quimioterápico Acetilcolinesterase Peroxidação lipídica Ratos Anabolic androgenic steroid Chemotherapeutic Acetylcholinesterase Lipid peroxidation Rats CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
title_short |
Sistema colinérgico e peroxidação lipídica de ratos tratados com sulfato de vincristina e decanoato de nandrolona |
title_full |
Sistema colinérgico e peroxidação lipídica de ratos tratados com sulfato de vincristina e decanoato de nandrolona |
title_fullStr |
Sistema colinérgico e peroxidação lipídica de ratos tratados com sulfato de vincristina e decanoato de nandrolona |
title_full_unstemmed |
Sistema colinérgico e peroxidação lipídica de ratos tratados com sulfato de vincristina e decanoato de nandrolona |
title_sort |
Sistema colinérgico e peroxidação lipídica de ratos tratados com sulfato de vincristina e decanoato de nandrolona |
author |
Martins, Danieli Brolo |
author_facet |
Martins, Danieli Brolo |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Lopes, Sonia Terezinha dos Anjos |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/8059723754130756 |
dc.contributor.referee1.fl_str_mv |
Schetinger, Maria Rosa Chitolina |
dc.contributor.referee2.fl_str_mv |
Lago, Lissandra Dal |
dc.contributor.referee2Lattes.fl_str_mv |
http://lattes.cnpq.br/7448358134461750 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/2159963349521553 |
dc.contributor.author.fl_str_mv |
Martins, Danieli Brolo |
contributor_str_mv |
Lopes, Sonia Terezinha dos Anjos Schetinger, Maria Rosa Chitolina Lago, Lissandra Dal |
dc.subject.por.fl_str_mv |
Esteróide anabólico androgênico Quimioterápico Acetilcolinesterase Peroxidação lipídica Ratos |
topic |
Esteróide anabólico androgênico Quimioterápico Acetilcolinesterase Peroxidação lipídica Ratos Anabolic androgenic steroid Chemotherapeutic Acetylcholinesterase Lipid peroxidation Rats CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
dc.subject.eng.fl_str_mv |
Anabolic androgenic steroid Chemotherapeutic Acetylcholinesterase Lipid peroxidation Rats |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
description |
Vincristine sulphate is antitumor agent widely used in small animal clinical oncology; therefore it can cause a number of adverse effects including marrow and neuronal cytotoxicity. Nandrolone decanoate, an anabolic-androgenic steroid (AAS), has been used in association with vincristine in order to ease effects such as moderate myelosuppression. The present dissertation presents data related to the isolated or associated employment of vincristine sulphate and nandrolone decanoate, as well as their effects on the cholinergic system and oxidative profile of Wistar rats. The animals were submitted to four different doses of AAS for three weeks and its action on acetylcholinesterase (AChE) activity in four different structures of brain tissue (cerebellum, hippocampus, striatum and cerebral cortex) was studied. The second experiment shows the effects of vincristine and/or nandrolone decanoate in the brain (same parts studied previously) and blood through measurement of brain and red blood cell AChE (RBC-AChE) enzyme activity as well as brain and blood serum lipid peroxidation of rats treated for two weeks. Results show that the two highest doses used in the first experiment increased enzyme activity, suggesting interference in the cholinergical system of the striatum and cerebellum. The results obtained in the latter experiment demonstrate that the isolated use of this AAS and its association with vincristine sulphate altered brain and RBC-AChE action, both in a stimulatory and inhibitory fashion. Lipid peroxidation, in the brain and blood, increased due to the isolated use of both vincristine and nandrolone decanoate, as well as to their associated use at the highest dose of ester used. Furthermore, the data show that the association between the therapeutic dose of nandrolone decanoate and vincristine is capable of neutralizing the free radical production induced by their isolated use in brain and blood serum. Serum RBC-AChE activity and the oxidative profile presented in this study are similar to those exhibited for brain tissue. Based on these data, it can be concluded that nandrolone decanoate is capable of interfering in AChE activity, affecting the cholinergic system, which could cause an alteration of its neurotransmitter, as well as a low or high stimulation of post-synaptic receptors. Therefore, the use of the therapeutic dose of AAS studied here in association with vincristine has been shown to be beneficial, as it could protect the organism from damaging processes caused by the production of free radicals. |
publishDate |
2008 |
dc.date.accessioned.fl_str_mv |
2008-03-27 |
dc.date.available.fl_str_mv |
2008-03-27 |
dc.date.issued.fl_str_mv |
2008-02-22 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
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masterThesis |
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publishedVersion |
dc.identifier.citation.fl_str_mv |
MARTINS, Danieli Brolo. Evaluation of cholinergic system and lipid peroxidation of rats treated with vincristine sulphate and nandrolone decanoate. 2008. 86 f. Dissertação (Mestrado em Medicina Veterinária) - Universidade Federal de Santa Maria, Santa Maria, 2008. |
dc.identifier.uri.fl_str_mv |
http://repositorio.ufsm.br/handle/1/10011 |
identifier_str_mv |
MARTINS, Danieli Brolo. Evaluation of cholinergic system and lipid peroxidation of rats treated with vincristine sulphate and nandrolone decanoate. 2008. 86 f. Dissertação (Mestrado em Medicina Veterinária) - Universidade Federal de Santa Maria, Santa Maria, 2008. |
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http://repositorio.ufsm.br/handle/1/10011 |
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Universidade Federal de Santa Maria |
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UFSM |
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Medicina Veterinária |
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Universidade Federal de Santa Maria |
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