Evaluating and Improving Vitek MS for Identification of Clinically Relevant Species of Trichosporon and the Closely Related Genera Cutaneotrichosporon and Apiotrichum
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://repositorio.unifesp.br/handle/11600/51441 http://dx.doi.org/10.1128/JCM.00461-17 |
Resumo: | Trichosporon species are relevant etiologic agents of hospital-acquired infections. High mortality rates are attributed to Trichosporon deep-seated infections in immuno-compromised individuals, making fast and accurate species identification relevant for hastening the discovery of best-targeted therapy. Recently, Trichosporon taxonomy has been reassessed, and three genera have been proposed for the pathogenic species: Trichosporon, Cutaneotrichosporon, and Apiotrichum. Matrix-assisted laser desorption ionization- time of flight mass spectrometry (MALDI-TOF MS) has replaced old phenotypic methods for microorganism identification in clinical laboratories, but spectral profile databases have to be evaluated and improved for optimal species identification performance. Vitek MS (bioMerieux) is one of the commercially available MALDI-TOF MS platforms for pathogen identification, and its spectral profile databases remain poorly evaluated for Trichosporon, Cutaneotrichosporon, and Apiotrichum species identification. We herein evaluated and improved Vitek MS for the identification of the main clinical relevant species of Trichosporon, Cutaneotrichosporon, and Apiotrichum using a large set of strains and isolates belonging to different yeast collections in Brazil and France. |
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de Almeida, Joao N., Jr.Favero Gimenes, Viviane M.Francisco, Elaine C. [UNIFESP]Machado Siqueira, Lumena P.Goncalves de Almeida, Renato K.Guitard, JulietteHennequin, ChristopheColombo, Arnaldo L. [UNIFESP]Benard, GilRossi, Flavia2019-08-19T11:49:56Z2019-08-19T11:49:56Z2017Journal Of Clinical Microbiology. Washington, v. 55, n. 8, p. 2439-2444, 2017.0095-1137http://repositorio.unifesp.br/handle/11600/51441http://dx.doi.org/10.1128/JCM.00461-1710.1128/JCM.00461-17WOS:000408109800019Trichosporon species are relevant etiologic agents of hospital-acquired infections. High mortality rates are attributed to Trichosporon deep-seated infections in immuno-compromised individuals, making fast and accurate species identification relevant for hastening the discovery of best-targeted therapy. Recently, Trichosporon taxonomy has been reassessed, and three genera have been proposed for the pathogenic species: Trichosporon, Cutaneotrichosporon, and Apiotrichum. Matrix-assisted laser desorption ionization- time of flight mass spectrometry (MALDI-TOF MS) has replaced old phenotypic methods for microorganism identification in clinical laboratories, but spectral profile databases have to be evaluated and improved for optimal species identification performance. Vitek MS (bioMerieux) is one of the commercially available MALDI-TOF MS platforms for pathogen identification, and its spectral profile databases remain poorly evaluated for Trichosporon, Cutaneotrichosporon, and Apiotrichum species identification. We herein evaluated and improved Vitek MS for the identification of the main clinical relevant species of Trichosporon, Cutaneotrichosporon, and Apiotrichum using a large set of strains and isolates belonging to different yeast collections in Brazil and France.National Council of Technological and Scientific Development (CNPq)Univ São Paulo, Fac Med, Hosp Clin, Cent Lab Div LIM03, São Paulo, BrazilUniv São Paulo, Inst Med Trop São Paulo, Lab Mycol LIM 53, São Paulo, BrazilUniv Fed São Paulo, Escola Paulista Med, Lab Especial Micol, São Paulo, BrazilHop St Antoine, AP HP, Serv Parasitol Mycol, Paris, FranceUPMC Univ Paris 06, INSERM, CNRS, UMR 1135,Sorbonne Univ,ERL 8255, Paris, FranceCtr Immunol & Malad Infect CIMI Paris, Paris, FranceUniv Fed São Paulo, Escola Paulista Med, Lab Especial Micol, São Paulo, BrazilCNPq: 307510/2015-8Web of Science2439-2444engAmer Soc MicrobiologyTrichosporonCutaneotrichosporonApiotrichumVitek MMALDI-TOF mass spectrometryEvaluating and Improving Vitek MS for Identification of Clinically Relevant Species of Trichosporon and the Closely Related Genera Cutaneotrichosporon and Apiotrichuminfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP11600/514412022-06-02 09:20:43.959metadata only accessoai:repositorio.unifesp.br:11600/51441Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652023-05-25T12:15:06.857708Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.en.fl_str_mv |
Evaluating and Improving Vitek MS for Identification of Clinically Relevant Species of Trichosporon and the Closely Related Genera Cutaneotrichosporon and Apiotrichum |
title |
Evaluating and Improving Vitek MS for Identification of Clinically Relevant Species of Trichosporon and the Closely Related Genera Cutaneotrichosporon and Apiotrichum |
spellingShingle |
Evaluating and Improving Vitek MS for Identification of Clinically Relevant Species of Trichosporon and the Closely Related Genera Cutaneotrichosporon and Apiotrichum de Almeida, Joao N., Jr. Trichosporon Cutaneotrichosporon Apiotrichum Vitek M MALDI-TOF mass spectrometry |
title_short |
Evaluating and Improving Vitek MS for Identification of Clinically Relevant Species of Trichosporon and the Closely Related Genera Cutaneotrichosporon and Apiotrichum |
title_full |
Evaluating and Improving Vitek MS for Identification of Clinically Relevant Species of Trichosporon and the Closely Related Genera Cutaneotrichosporon and Apiotrichum |
title_fullStr |
Evaluating and Improving Vitek MS for Identification of Clinically Relevant Species of Trichosporon and the Closely Related Genera Cutaneotrichosporon and Apiotrichum |
title_full_unstemmed |
Evaluating and Improving Vitek MS for Identification of Clinically Relevant Species of Trichosporon and the Closely Related Genera Cutaneotrichosporon and Apiotrichum |
title_sort |
Evaluating and Improving Vitek MS for Identification of Clinically Relevant Species of Trichosporon and the Closely Related Genera Cutaneotrichosporon and Apiotrichum |
author |
de Almeida, Joao N., Jr. |
author_facet |
de Almeida, Joao N., Jr. Favero Gimenes, Viviane M. Francisco, Elaine C. [UNIFESP] Machado Siqueira, Lumena P. Goncalves de Almeida, Renato K. Guitard, Juliette Hennequin, Christophe Colombo, Arnaldo L. [UNIFESP] Benard, Gil Rossi, Flavia |
author_role |
author |
author2 |
Favero Gimenes, Viviane M. Francisco, Elaine C. [UNIFESP] Machado Siqueira, Lumena P. Goncalves de Almeida, Renato K. Guitard, Juliette Hennequin, Christophe Colombo, Arnaldo L. [UNIFESP] Benard, Gil Rossi, Flavia |
author2_role |
author author author author author author author author author |
dc.contributor.author.fl_str_mv |
de Almeida, Joao N., Jr. Favero Gimenes, Viviane M. Francisco, Elaine C. [UNIFESP] Machado Siqueira, Lumena P. Goncalves de Almeida, Renato K. Guitard, Juliette Hennequin, Christophe Colombo, Arnaldo L. [UNIFESP] Benard, Gil Rossi, Flavia |
dc.subject.eng.fl_str_mv |
Trichosporon Cutaneotrichosporon Apiotrichum Vitek M MALDI-TOF mass spectrometry |
topic |
Trichosporon Cutaneotrichosporon Apiotrichum Vitek M MALDI-TOF mass spectrometry |
description |
Trichosporon species are relevant etiologic agents of hospital-acquired infections. High mortality rates are attributed to Trichosporon deep-seated infections in immuno-compromised individuals, making fast and accurate species identification relevant for hastening the discovery of best-targeted therapy. Recently, Trichosporon taxonomy has been reassessed, and three genera have been proposed for the pathogenic species: Trichosporon, Cutaneotrichosporon, and Apiotrichum. Matrix-assisted laser desorption ionization- time of flight mass spectrometry (MALDI-TOF MS) has replaced old phenotypic methods for microorganism identification in clinical laboratories, but spectral profile databases have to be evaluated and improved for optimal species identification performance. Vitek MS (bioMerieux) is one of the commercially available MALDI-TOF MS platforms for pathogen identification, and its spectral profile databases remain poorly evaluated for Trichosporon, Cutaneotrichosporon, and Apiotrichum species identification. We herein evaluated and improved Vitek MS for the identification of the main clinical relevant species of Trichosporon, Cutaneotrichosporon, and Apiotrichum using a large set of strains and isolates belonging to different yeast collections in Brazil and France. |
publishDate |
2017 |
dc.date.issued.fl_str_mv |
2017 |
dc.date.accessioned.fl_str_mv |
2019-08-19T11:49:56Z |
dc.date.available.fl_str_mv |
2019-08-19T11:49:56Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
Journal Of Clinical Microbiology. Washington, v. 55, n. 8, p. 2439-2444, 2017. |
dc.identifier.uri.fl_str_mv |
http://repositorio.unifesp.br/handle/11600/51441 http://dx.doi.org/10.1128/JCM.00461-17 |
dc.identifier.issn.none.fl_str_mv |
0095-1137 |
dc.identifier.doi.none.fl_str_mv |
10.1128/JCM.00461-17 |
dc.identifier.wos.none.fl_str_mv |
WOS:000408109800019 |
identifier_str_mv |
Journal Of Clinical Microbiology. Washington, v. 55, n. 8, p. 2439-2444, 2017. 0095-1137 10.1128/JCM.00461-17 WOS:000408109800019 |
url |
http://repositorio.unifesp.br/handle/11600/51441 http://dx.doi.org/10.1128/JCM.00461-17 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
2439-2444 |
dc.publisher.none.fl_str_mv |
Amer Soc Microbiology |
publisher.none.fl_str_mv |
Amer Soc Microbiology |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
instname_str |
Universidade Federal de São Paulo (UNIFESP) |
instacron_str |
UNIFESP |
institution |
UNIFESP |
reponame_str |
Repositório Institucional da UNIFESP |
collection |
Repositório Institucional da UNIFESP |
repository.name.fl_str_mv |
Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
repository.mail.fl_str_mv |
|
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1783460235924471808 |