Comparing the phenotypic, genotypic, and proteomic identification of Trichosporon species: A globally emerging yeast of medical importance
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1093/mmy/myab050 http://hdl.handle.net/11449/223182 |
Resumo: | Trichosporon spp. are widely distributed in the nature, comprising species that inhabit different ecological niches and can be found in the water, soil, and body surface of animals and humans. Such microorganisms have been classically associated with superficial infections; however, in the last decades, they have also been related to disseminated infections in immunocompromised patients, behaving as opportunistic agents, which demands rapid and accurate species identification for efficient therapy. Concordance level between the traditional phenotypic method and the molecular technique (gold standard) in the identification of all 59 Trichosporon samples was 59.3%. Identification concordance between MALDI-TOF spectrometry and the molecular technique was 71.2%. No isolate of environmental origin was identifiable by MALDI-TOF mass spectrometry (MS), and 100% of such environmental isolates were discordant for IGS region sequencing and phenotypic characterization. Both comparisons evidenced greatest concordance in the identification of T. asahii. The species T. debeurmannianum, T. dermatis, T. venhuisii and T. insectorum were not properly identified by both MALDI-TOF MS and the phenotypic technique. MALDI-TOF MS, in particular, seems to be appropriate to investigate yeasts of the genus Trichosporon; however, database updates are still necessary, especially for species that are not common in the clinical routine. With the aim of helping understand the aspects involved in early and accurate diagnosis of infections caused by this opportunistic agent, the present study compared the phenotypic, molecular (IGS region) and mass-spectrometry (MALDI-TOF) identification of 59 yeasts of the genus Trichosporon which had clinical and environmental origin and were kept in a mycology collection. |
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Comparing the phenotypic, genotypic, and proteomic identification of Trichosporon species: A globally emerging yeast of medical importanceEmerging pathogensGenotypic identificationMALDI-TOF MSPhenotypic identificationTrichosporon sppTrichosporon spp. are widely distributed in the nature, comprising species that inhabit different ecological niches and can be found in the water, soil, and body surface of animals and humans. Such microorganisms have been classically associated with superficial infections; however, in the last decades, they have also been related to disseminated infections in immunocompromised patients, behaving as opportunistic agents, which demands rapid and accurate species identification for efficient therapy. Concordance level between the traditional phenotypic method and the molecular technique (gold standard) in the identification of all 59 Trichosporon samples was 59.3%. Identification concordance between MALDI-TOF spectrometry and the molecular technique was 71.2%. No isolate of environmental origin was identifiable by MALDI-TOF mass spectrometry (MS), and 100% of such environmental isolates were discordant for IGS region sequencing and phenotypic characterization. Both comparisons evidenced greatest concordance in the identification of T. asahii. The species T. debeurmannianum, T. dermatis, T. venhuisii and T. insectorum were not properly identified by both MALDI-TOF MS and the phenotypic technique. MALDI-TOF MS, in particular, seems to be appropriate to investigate yeasts of the genus Trichosporon; however, database updates are still necessary, especially for species that are not common in the clinical routine. With the aim of helping understand the aspects involved in early and accurate diagnosis of infections caused by this opportunistic agent, the present study compared the phenotypic, molecular (IGS region) and mass-spectrometry (MALDI-TOF) identification of 59 yeasts of the genus Trichosporon which had clinical and environmental origin and were kept in a mycology collection.São Paulo State University (Unesp) Institute of Biosciences, São Paulo (SP)Adolfo Lutz Institute (IAL) CLR II Biomedical Sciences Center, SPUniversity of São Paulo (USP) School of Dentistry, SPFaculty of Medicine Federal University of Mato Grosso (UFMT), Av. Fernando Corrêa da Costa, 2.367, MTIrmandade da Santa Casa de Misericórdia de São Paulo Hospital, SPDarcy Vargas Children's Hospital, SPBauru State Hospital (HEB), SPUniversity of São Paulo (USP) Institute of Tropical Medicine, SPAdolfo Lutz Central Institute (IAL) Mycology Sector, SPSão Paulo State University (Unesp) Institute of Biosciences, São Paulo (SP)Universidade Estadual Paulista (UNESP)Biomedical Sciences CenterUniversidade de São Paulo (USP)Federal University of Mato Grosso (UFMT)HospitalDarcy Vargas Children's HospitalBauru State Hospital (HEB)Mycology SectorLara, Bruna Rossini [UNESP]De Camargo, Bruno BraidottiPaula, Claudete RodriguesJunior, Diniz Pereira LeiteGarces, Hans Garcia [UNESP]Arnoni, Mariana VolpeSilveira, MônicaGimenes, Viviane Mazo FáveroSiqueira, Lumena Pereira MacHadoTakahashi, Juliana Possatto FernandesMelhem, Márcia De Souza CarvalhoRichini-Pereira, Virgínia BodelãoAnversa, LaísRuiz, Luciana Da Silva2022-04-28T19:49:05Z2022-04-28T19:49:05Z2021-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1181-1190http://dx.doi.org/10.1093/mmy/myab050Medical Mycology, v. 59, n. 12, p. 1181-1190, 2021.1460-27091369-3786http://hdl.handle.net/11449/22318210.1093/mmy/myab0502-s2.0-85122344941Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMedical Mycologyinfo:eu-repo/semantics/openAccess2022-04-28T19:49:05Zoai:repositorio.unesp.br:11449/223182Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462022-04-28T19:49:05Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Comparing the phenotypic, genotypic, and proteomic identification of Trichosporon species: A globally emerging yeast of medical importance |
title |
Comparing the phenotypic, genotypic, and proteomic identification of Trichosporon species: A globally emerging yeast of medical importance |
spellingShingle |
Comparing the phenotypic, genotypic, and proteomic identification of Trichosporon species: A globally emerging yeast of medical importance Lara, Bruna Rossini [UNESP] Emerging pathogens Genotypic identification MALDI-TOF MS Phenotypic identification Trichosporon spp |
title_short |
Comparing the phenotypic, genotypic, and proteomic identification of Trichosporon species: A globally emerging yeast of medical importance |
title_full |
Comparing the phenotypic, genotypic, and proteomic identification of Trichosporon species: A globally emerging yeast of medical importance |
title_fullStr |
Comparing the phenotypic, genotypic, and proteomic identification of Trichosporon species: A globally emerging yeast of medical importance |
title_full_unstemmed |
Comparing the phenotypic, genotypic, and proteomic identification of Trichosporon species: A globally emerging yeast of medical importance |
title_sort |
Comparing the phenotypic, genotypic, and proteomic identification of Trichosporon species: A globally emerging yeast of medical importance |
author |
Lara, Bruna Rossini [UNESP] |
author_facet |
Lara, Bruna Rossini [UNESP] De Camargo, Bruno Braidotti Paula, Claudete Rodrigues Junior, Diniz Pereira Leite Garces, Hans Garcia [UNESP] Arnoni, Mariana Volpe Silveira, Mônica Gimenes, Viviane Mazo Fávero Siqueira, Lumena Pereira MacHado Takahashi, Juliana Possatto Fernandes Melhem, Márcia De Souza Carvalho Richini-Pereira, Virgínia Bodelão Anversa, Laís Ruiz, Luciana Da Silva |
author_role |
author |
author2 |
De Camargo, Bruno Braidotti Paula, Claudete Rodrigues Junior, Diniz Pereira Leite Garces, Hans Garcia [UNESP] Arnoni, Mariana Volpe Silveira, Mônica Gimenes, Viviane Mazo Fávero Siqueira, Lumena Pereira MacHado Takahashi, Juliana Possatto Fernandes Melhem, Márcia De Souza Carvalho Richini-Pereira, Virgínia Bodelão Anversa, Laís Ruiz, Luciana Da Silva |
author2_role |
author author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) Biomedical Sciences Center Universidade de São Paulo (USP) Federal University of Mato Grosso (UFMT) Hospital Darcy Vargas Children's Hospital Bauru State Hospital (HEB) Mycology Sector |
dc.contributor.author.fl_str_mv |
Lara, Bruna Rossini [UNESP] De Camargo, Bruno Braidotti Paula, Claudete Rodrigues Junior, Diniz Pereira Leite Garces, Hans Garcia [UNESP] Arnoni, Mariana Volpe Silveira, Mônica Gimenes, Viviane Mazo Fávero Siqueira, Lumena Pereira MacHado Takahashi, Juliana Possatto Fernandes Melhem, Márcia De Souza Carvalho Richini-Pereira, Virgínia Bodelão Anversa, Laís Ruiz, Luciana Da Silva |
dc.subject.por.fl_str_mv |
Emerging pathogens Genotypic identification MALDI-TOF MS Phenotypic identification Trichosporon spp |
topic |
Emerging pathogens Genotypic identification MALDI-TOF MS Phenotypic identification Trichosporon spp |
description |
Trichosporon spp. are widely distributed in the nature, comprising species that inhabit different ecological niches and can be found in the water, soil, and body surface of animals and humans. Such microorganisms have been classically associated with superficial infections; however, in the last decades, they have also been related to disseminated infections in immunocompromised patients, behaving as opportunistic agents, which demands rapid and accurate species identification for efficient therapy. Concordance level between the traditional phenotypic method and the molecular technique (gold standard) in the identification of all 59 Trichosporon samples was 59.3%. Identification concordance between MALDI-TOF spectrometry and the molecular technique was 71.2%. No isolate of environmental origin was identifiable by MALDI-TOF mass spectrometry (MS), and 100% of such environmental isolates were discordant for IGS region sequencing and phenotypic characterization. Both comparisons evidenced greatest concordance in the identification of T. asahii. The species T. debeurmannianum, T. dermatis, T. venhuisii and T. insectorum were not properly identified by both MALDI-TOF MS and the phenotypic technique. MALDI-TOF MS, in particular, seems to be appropriate to investigate yeasts of the genus Trichosporon; however, database updates are still necessary, especially for species that are not common in the clinical routine. With the aim of helping understand the aspects involved in early and accurate diagnosis of infections caused by this opportunistic agent, the present study compared the phenotypic, molecular (IGS region) and mass-spectrometry (MALDI-TOF) identification of 59 yeasts of the genus Trichosporon which had clinical and environmental origin and were kept in a mycology collection. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-12-01 2022-04-28T19:49:05Z 2022-04-28T19:49:05Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1093/mmy/myab050 Medical Mycology, v. 59, n. 12, p. 1181-1190, 2021. 1460-2709 1369-3786 http://hdl.handle.net/11449/223182 10.1093/mmy/myab050 2-s2.0-85122344941 |
url |
http://dx.doi.org/10.1093/mmy/myab050 http://hdl.handle.net/11449/223182 |
identifier_str_mv |
Medical Mycology, v. 59, n. 12, p. 1181-1190, 2021. 1460-2709 1369-3786 10.1093/mmy/myab050 2-s2.0-85122344941 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Medical Mycology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1181-1190 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1797789709424918528 |