Evidence that ATP participates in the pathophysiology of pilocarpine-induced temporal lobe epilepsy: Fluorometric, immunohistochemical, and Western blot studies

Detalhes bibliográficos
Autor(a) principal: Vianna, Eduardo Paulo Morawski [UNIFESP]
Data de Publicação: 2002
Outros Autores: Ferreira, Alice Teixeira [UNIFESP], Naffah-Mazzacoratti, Maria da Graca [UNIFESP], Sanabria, Emilio Rafael Garrido [UNIFESP], Funke, M. [UNIFESP], Cavalheiro, Esper Abrão [UNIFESP], Fernandes, Maria Jose da Silva [UNIFESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNIFESP
Texto Completo: http://repositorio.unifesp.br/handle/11600/26681
http://dx.doi.org/10.1046/j.1528-1157.43.s.5.26.x
Resumo: Purpose: This study was performed to study the role of adenosine triphosphate (ATP) in the brain of pilocarpine-induced chronic epileptic rats.Methods: ATP-mediated changes in intracellular calcium were studied by the fura-2 method. lmmunohistochemistry and Western blotting methods were used to localize and quantify P2X7 receptors in these animals.Results: the fluorometric study in chronic rats revealed a biphasic response indicating the presence of P2X7 receptors. the Western blotting study showed an increase of 80% of P2X7 expression in chronic rats compared with the control group. P2X7 immunoreactivity resembled mossy fiber sprouting at the dentate gyrus of epileptic animals.Conclusions: These results suggest that purinergic receptors may participate in the pathophysiology of temporal lobe epilepsy.
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spelling Vianna, Eduardo Paulo Morawski [UNIFESP]Ferreira, Alice Teixeira [UNIFESP]Naffah-Mazzacoratti, Maria da Graca [UNIFESP]Sanabria, Emilio Rafael Garrido [UNIFESP]Funke, M. [UNIFESP]Cavalheiro, Esper Abrão [UNIFESP]Fernandes, Maria Jose da Silva [UNIFESP]Universidade Federal de São Paulo (UNIFESP)2016-01-24T12:33:09Z2016-01-24T12:33:09Z2002-01-01Epilepsia. Malden: Blackwell Publishing Inc, v. 43, p. 227-229, 2002.0013-9580http://repositorio.unifesp.br/handle/11600/26681http://dx.doi.org/10.1046/j.1528-1157.43.s.5.26.x10.1046/j.1528-1157.43.s.5.26.xWOS:000177445900042Purpose: This study was performed to study the role of adenosine triphosphate (ATP) in the brain of pilocarpine-induced chronic epileptic rats.Methods: ATP-mediated changes in intracellular calcium were studied by the fura-2 method. lmmunohistochemistry and Western blotting methods were used to localize and quantify P2X7 receptors in these animals.Results: the fluorometric study in chronic rats revealed a biphasic response indicating the presence of P2X7 receptors. the Western blotting study showed an increase of 80% of P2X7 expression in chronic rats compared with the control group. P2X7 immunoreactivity resembled mossy fiber sprouting at the dentate gyrus of epileptic animals.Conclusions: These results suggest that purinergic receptors may participate in the pathophysiology of temporal lobe epilepsy.Universidade Federal de São Paulo, Disciplina Neurol Expt, Dept Neurol Neurocirurg, BR-04023900 São Paulo, BrazilUniversidade Federal de São Paulo, Dept Biofis, BR-04023900 São Paulo, BrazilUniversidade Federal de São Paulo, Disciplina Neurol Expt, Dept Neurol Neurocirurg, BR-04023900 São Paulo, BrazilUniversidade Federal de São Paulo, Dept Biofis, BR-04023900 São Paulo, BrazilWeb of Science227-229engBlackwell Publishing IncEpilepsiaepilepsyATPP2X7pilocarpineratEvidence that ATP participates in the pathophysiology of pilocarpine-induced temporal lobe epilepsy: Fluorometric, immunohistochemical, and Western blot studiesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP11600/266812022-07-08 10:51:09.751metadata only accessoai:repositorio.unifesp.br:11600/26681Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652023-05-25T12:20:32.504860Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.en.fl_str_mv Evidence that ATP participates in the pathophysiology of pilocarpine-induced temporal lobe epilepsy: Fluorometric, immunohistochemical, and Western blot studies
title Evidence that ATP participates in the pathophysiology of pilocarpine-induced temporal lobe epilepsy: Fluorometric, immunohistochemical, and Western blot studies
spellingShingle Evidence that ATP participates in the pathophysiology of pilocarpine-induced temporal lobe epilepsy: Fluorometric, immunohistochemical, and Western blot studies
Vianna, Eduardo Paulo Morawski [UNIFESP]
epilepsy
ATP
P2X7
pilocarpine
rat
title_short Evidence that ATP participates in the pathophysiology of pilocarpine-induced temporal lobe epilepsy: Fluorometric, immunohistochemical, and Western blot studies
title_full Evidence that ATP participates in the pathophysiology of pilocarpine-induced temporal lobe epilepsy: Fluorometric, immunohistochemical, and Western blot studies
title_fullStr Evidence that ATP participates in the pathophysiology of pilocarpine-induced temporal lobe epilepsy: Fluorometric, immunohistochemical, and Western blot studies
title_full_unstemmed Evidence that ATP participates in the pathophysiology of pilocarpine-induced temporal lobe epilepsy: Fluorometric, immunohistochemical, and Western blot studies
title_sort Evidence that ATP participates in the pathophysiology of pilocarpine-induced temporal lobe epilepsy: Fluorometric, immunohistochemical, and Western blot studies
author Vianna, Eduardo Paulo Morawski [UNIFESP]
author_facet Vianna, Eduardo Paulo Morawski [UNIFESP]
Ferreira, Alice Teixeira [UNIFESP]
Naffah-Mazzacoratti, Maria da Graca [UNIFESP]
Sanabria, Emilio Rafael Garrido [UNIFESP]
Funke, M. [UNIFESP]
Cavalheiro, Esper Abrão [UNIFESP]
Fernandes, Maria Jose da Silva [UNIFESP]
author_role author
author2 Ferreira, Alice Teixeira [UNIFESP]
Naffah-Mazzacoratti, Maria da Graca [UNIFESP]
Sanabria, Emilio Rafael Garrido [UNIFESP]
Funke, M. [UNIFESP]
Cavalheiro, Esper Abrão [UNIFESP]
Fernandes, Maria Jose da Silva [UNIFESP]
author2_role author
author
author
author
author
author
dc.contributor.institution.none.fl_str_mv Universidade Federal de São Paulo (UNIFESP)
dc.contributor.author.fl_str_mv Vianna, Eduardo Paulo Morawski [UNIFESP]
Ferreira, Alice Teixeira [UNIFESP]
Naffah-Mazzacoratti, Maria da Graca [UNIFESP]
Sanabria, Emilio Rafael Garrido [UNIFESP]
Funke, M. [UNIFESP]
Cavalheiro, Esper Abrão [UNIFESP]
Fernandes, Maria Jose da Silva [UNIFESP]
dc.subject.eng.fl_str_mv epilepsy
ATP
P2X7
pilocarpine
rat
topic epilepsy
ATP
P2X7
pilocarpine
rat
description Purpose: This study was performed to study the role of adenosine triphosphate (ATP) in the brain of pilocarpine-induced chronic epileptic rats.Methods: ATP-mediated changes in intracellular calcium were studied by the fura-2 method. lmmunohistochemistry and Western blotting methods were used to localize and quantify P2X7 receptors in these animals.Results: the fluorometric study in chronic rats revealed a biphasic response indicating the presence of P2X7 receptors. the Western blotting study showed an increase of 80% of P2X7 expression in chronic rats compared with the control group. P2X7 immunoreactivity resembled mossy fiber sprouting at the dentate gyrus of epileptic animals.Conclusions: These results suggest that purinergic receptors may participate in the pathophysiology of temporal lobe epilepsy.
publishDate 2002
dc.date.issued.fl_str_mv 2002-01-01
dc.date.accessioned.fl_str_mv 2016-01-24T12:33:09Z
dc.date.available.fl_str_mv 2016-01-24T12:33:09Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv Epilepsia. Malden: Blackwell Publishing Inc, v. 43, p. 227-229, 2002.
dc.identifier.uri.fl_str_mv http://repositorio.unifesp.br/handle/11600/26681
http://dx.doi.org/10.1046/j.1528-1157.43.s.5.26.x
dc.identifier.issn.none.fl_str_mv 0013-9580
dc.identifier.doi.none.fl_str_mv 10.1046/j.1528-1157.43.s.5.26.x
dc.identifier.wos.none.fl_str_mv WOS:000177445900042
identifier_str_mv Epilepsia. Malden: Blackwell Publishing Inc, v. 43, p. 227-229, 2002.
0013-9580
10.1046/j.1528-1157.43.s.5.26.x
WOS:000177445900042
url http://repositorio.unifesp.br/handle/11600/26681
http://dx.doi.org/10.1046/j.1528-1157.43.s.5.26.x
dc.language.iso.fl_str_mv eng
language eng
dc.relation.ispartof.none.fl_str_mv Epilepsia
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 227-229
dc.publisher.none.fl_str_mv Blackwell Publishing Inc
publisher.none.fl_str_mv Blackwell Publishing Inc
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
repository.name.fl_str_mv Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)
repository.mail.fl_str_mv
_version_ 1783460278797598720

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