Estrogen effects on pilocarpine-induced temporal lobe epilepsy in rats

Detalhes bibliográficos
Autor(a) principal: Pereira Júnior, Melquíades [UNIFESP]
Data de Publicação: 2009
Outros Autores: Soares, Jose M. [UNIFESP], Valente, Sandra G. [UNIFESP], Oliveira, Patricia B. [UNIFESP], Cavalheiro, Esper A. [UNIFESP], Amado, Dbora [UNIFESP], Baracat, Edmund Chada [UNIFESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNIFESP
Texto Completo: http://repositorio.unifesp.br/handle/11600/31321
http://dx.doi.org/10.1016/j.maturitas.2008.10.014
Resumo: Objetive: To evaluate the effects of conjugated equine estrogens (CEE) on the pilocarpine-induced epilepsy in rats.Study design: 40 female rats were divided into: GPC (positive control) presented status epilepticus (SE) induced by pilocarpine; GOC(ovariectomized control) only castrated; GNC (negative control) received only saline solution; GPE received pilocarpine, presented SE, castrated and received 50 mu g/kg CEE treatment; GPV received pilocarpine, castrated and received propylene glycol (vehicle). the animals were monitored by a video system. At the end of observation, the brains removed for later histologic analysis using Neo-Timm and Nissl methods.Results: the GPE presented a reduction in number of seizures compared to GPV. the Neo-Timm analysis showed that GPV had greater sprouting of mossy fibers, with a denser band in the area of the dentate gyrus hilum compared to GPE. On Nissl staining, GPE showed evident neuronal loss in the CA3 area. GPV presented loss in CA1 and dentate gyrus.Conclusion: Estrogen may have a protecting effect on the central nervous system. (C) 2008 Elsevier Ireland Ltd. All rights reserved.
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spelling Pereira Júnior, Melquíades [UNIFESP]Soares, Jose M. [UNIFESP]Valente, Sandra G. [UNIFESP]Oliveira, Patricia B. [UNIFESP]Cavalheiro, Esper A. [UNIFESP]Amado, Dbora [UNIFESP]Baracat, Edmund Chada [UNIFESP]Universidade Federal de São Paulo (UNIFESP)Universidade de São Paulo (USP)2016-01-24T13:52:16Z2016-01-24T13:52:16Z2009-02-20Maturitas. Clare: Elsevier B.V., v. 62, n. 2, p. 190-196, 2009.0378-5122http://repositorio.unifesp.br/handle/11600/31321http://dx.doi.org/10.1016/j.maturitas.2008.10.01410.1016/j.maturitas.2008.10.014WOS:000264652800016Objetive: To evaluate the effects of conjugated equine estrogens (CEE) on the pilocarpine-induced epilepsy in rats.Study design: 40 female rats were divided into: GPC (positive control) presented status epilepticus (SE) induced by pilocarpine; GOC(ovariectomized control) only castrated; GNC (negative control) received only saline solution; GPE received pilocarpine, presented SE, castrated and received 50 mu g/kg CEE treatment; GPV received pilocarpine, castrated and received propylene glycol (vehicle). the animals were monitored by a video system. At the end of observation, the brains removed for later histologic analysis using Neo-Timm and Nissl methods.Results: the GPE presented a reduction in number of seizures compared to GPV. the Neo-Timm analysis showed that GPV had greater sprouting of mossy fibers, with a denser band in the area of the dentate gyrus hilum compared to GPE. On Nissl staining, GPE showed evident neuronal loss in the CA3 area. GPV presented loss in CA1 and dentate gyrus.Conclusion: Estrogen may have a protecting effect on the central nervous system. (C) 2008 Elsevier Ireland Ltd. All rights reserved.Universidade Federal de São Paulo, Escola Paulista Med, Dept Ginecol, BR-04021051 São Paulo, BrazilUniversidade Federal de São Paulo, Escola Paulista Med, Disciplina Neurol Expt, BR-04021051 São Paulo, BrazilUniv São Paulo, Dept Obstet & Ginecol, São Paulo, BrazilUniversidade Federal de São Paulo, Escola Paulista Med, Dept Ginecol, BR-04021051 São Paulo, BrazilUniversidade Federal de São Paulo, Escola Paulista Med, Disciplina Neurol Expt, BR-04021051 São Paulo, BrazilWeb of Science190-196engElsevier B.V.Maturitashttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policyinfo:eu-repo/semantics/openAccessExperimental modelsEpilepsyPilocarpineMenopauseEstrogenEstrogen effects on pilocarpine-induced temporal lobe epilepsy in ratsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlereponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP11600/313212022-06-02 09:35:06.293metadata only accessoai:repositorio.unifesp.br:11600/31321Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652023-05-25T12:16:22.510189Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.en.fl_str_mv Estrogen effects on pilocarpine-induced temporal lobe epilepsy in rats
title Estrogen effects on pilocarpine-induced temporal lobe epilepsy in rats
spellingShingle Estrogen effects on pilocarpine-induced temporal lobe epilepsy in rats
Pereira Júnior, Melquíades [UNIFESP]
Experimental models
Epilepsy
Pilocarpine
Menopause
Estrogen
title_short Estrogen effects on pilocarpine-induced temporal lobe epilepsy in rats
title_full Estrogen effects on pilocarpine-induced temporal lobe epilepsy in rats
title_fullStr Estrogen effects on pilocarpine-induced temporal lobe epilepsy in rats
title_full_unstemmed Estrogen effects on pilocarpine-induced temporal lobe epilepsy in rats
title_sort Estrogen effects on pilocarpine-induced temporal lobe epilepsy in rats
author Pereira Júnior, Melquíades [UNIFESP]
author_facet Pereira Júnior, Melquíades [UNIFESP]
Soares, Jose M. [UNIFESP]
Valente, Sandra G. [UNIFESP]
Oliveira, Patricia B. [UNIFESP]
Cavalheiro, Esper A. [UNIFESP]
Amado, Dbora [UNIFESP]
Baracat, Edmund Chada [UNIFESP]
author_role author
author2 Soares, Jose M. [UNIFESP]
Valente, Sandra G. [UNIFESP]
Oliveira, Patricia B. [UNIFESP]
Cavalheiro, Esper A. [UNIFESP]
Amado, Dbora [UNIFESP]
Baracat, Edmund Chada [UNIFESP]
author2_role author
author
author
author
author
author
dc.contributor.institution.none.fl_str_mv Universidade Federal de São Paulo (UNIFESP)
Universidade de São Paulo (USP)
dc.contributor.author.fl_str_mv Pereira Júnior, Melquíades [UNIFESP]
Soares, Jose M. [UNIFESP]
Valente, Sandra G. [UNIFESP]
Oliveira, Patricia B. [UNIFESP]
Cavalheiro, Esper A. [UNIFESP]
Amado, Dbora [UNIFESP]
Baracat, Edmund Chada [UNIFESP]
dc.subject.eng.fl_str_mv Experimental models
Epilepsy
Pilocarpine
Menopause
Estrogen
topic Experimental models
Epilepsy
Pilocarpine
Menopause
Estrogen
description Objetive: To evaluate the effects of conjugated equine estrogens (CEE) on the pilocarpine-induced epilepsy in rats.Study design: 40 female rats were divided into: GPC (positive control) presented status epilepticus (SE) induced by pilocarpine; GOC(ovariectomized control) only castrated; GNC (negative control) received only saline solution; GPE received pilocarpine, presented SE, castrated and received 50 mu g/kg CEE treatment; GPV received pilocarpine, castrated and received propylene glycol (vehicle). the animals were monitored by a video system. At the end of observation, the brains removed for later histologic analysis using Neo-Timm and Nissl methods.Results: the GPE presented a reduction in number of seizures compared to GPV. the Neo-Timm analysis showed that GPV had greater sprouting of mossy fibers, with a denser band in the area of the dentate gyrus hilum compared to GPE. On Nissl staining, GPE showed evident neuronal loss in the CA3 area. GPV presented loss in CA1 and dentate gyrus.Conclusion: Estrogen may have a protecting effect on the central nervous system. (C) 2008 Elsevier Ireland Ltd. All rights reserved.
publishDate 2009
dc.date.issued.fl_str_mv 2009-02-20
dc.date.accessioned.fl_str_mv 2016-01-24T13:52:16Z
dc.date.available.fl_str_mv 2016-01-24T13:52:16Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv Maturitas. Clare: Elsevier B.V., v. 62, n. 2, p. 190-196, 2009.
dc.identifier.uri.fl_str_mv http://repositorio.unifesp.br/handle/11600/31321
http://dx.doi.org/10.1016/j.maturitas.2008.10.014
dc.identifier.issn.none.fl_str_mv 0378-5122
dc.identifier.doi.none.fl_str_mv 10.1016/j.maturitas.2008.10.014
dc.identifier.wos.none.fl_str_mv WOS:000264652800016
identifier_str_mv Maturitas. Clare: Elsevier B.V., v. 62, n. 2, p. 190-196, 2009.
0378-5122
10.1016/j.maturitas.2008.10.014
WOS:000264652800016
url http://repositorio.unifesp.br/handle/11600/31321
http://dx.doi.org/10.1016/j.maturitas.2008.10.014
dc.language.iso.fl_str_mv eng
language eng
dc.relation.ispartof.none.fl_str_mv Maturitas
dc.rights.driver.fl_str_mv http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
info:eu-repo/semantics/openAccess
rights_invalid_str_mv http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 190-196
dc.publisher.none.fl_str_mv Elsevier B.V.
publisher.none.fl_str_mv Elsevier B.V.
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
repository.name.fl_str_mv Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)
repository.mail.fl_str_mv
_version_ 1783460270283161600

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