Low-level laser therapy modulates musculoskeletal loss in a skin burn model in rats

• Main Author: Martins, Francielle
• Publication Date: 2015
• Other Authors: Renno, Ana Claudia Muniz [UNIFESP], De Oliveira, Flavia [UNIFESP], Minatel, Natália Peruchi, Bortolin, Jeferson André, Quintana, Hananiah Tardivo, Aveiro, Mariana Chaves [UNIFESP]
• Format: Article
• Language: eng
• Source: Repositório Institucional da UNIFESP
• Download full: http://repositorio.unifesp.br/handle/11600/8793; http://dx.doi.org/10.1590/S0102-86502015002000002
• topic: Burns; Laser Therapy, Low-Level; MyoD Protein; Rats

PURPOSE: To investigate the effectiveness of low-level laser therapy (LLLT) on gastrocnemius muscle morphology and Myod imunoexpression in a model of dorsal burn in rats. METHODS: Sixteen male Wistar rats were distributed into two groups: control group (CG): rats submitted to scald burn injury without treatment and laser treated group (LG): rats submitted to scald burn injury and treated with laser therapy. Fourteen days post-surgery, gastrocnemius muscle was evaluated being the specimens stained with HE and morphometric data was evaluated. MyoD expression was assessed by immunohistochemistry. RESULTS: The results showed that laser treated animals presented more organized tissue morphology compared to the non-treated animals, with a higher number of nucleus in the fibers. Also, the cross sectional area of the fibers and the MyoD immunoexpression in the laser treated groups was higher. CONCLUSION: Low-level laser therapy had positive effects on gastrocnemius muscle, improving tissue muscle morphology, increasing cross sectional area and MyoD immunoexpression.