Somatic embryogenesis in macaw palm (Acrocomia aculeata) from zygotic embryos

Detalhes bibliográficos
Autor(a) principal: Moura, Elisa Ferreira
Data de Publicação: 2009
Outros Autores: Motoike, Sérgio Yoshimitsu, Ventrella, Marı́lia Contin, Sá Júnior, Adauto Quirino de, Carvalho, Mychelle
Tipo de documento: Artigo
Idioma: eng
Título da fonte: LOCUS Repositório Institucional da UFV
Texto Completo: https://doi.org/10.1016/j.scienta.2008.08.033
http://www.locus.ufv.br/handle/123456789/22380
Resumo: Macaw palm (Acrocomia aculeata) is an oleaginous palm tree that is highly productive and adapted to semiarid ecosystems, which oil can be used to produce biodiesel. Such characteristics make macaw palm a potential crop to be used by farmers from semi-arid regions, but its propagation is still problematic. This paper reports the first description of somatic embryogenesis for macaw palm from zygotic embryos. The explants were cultured on Y3 medium and different combinations of plant growth regulators. After 60 days of culture, embryogenic callus were induced with 9 μM of 4-amino-3,5,6-trichloropicolonic acid (picloram) or 2,4-dichlorophenoxyacetic acid (2,4-D), combined or not with 1 μM N-phenyl N′1,2,3 thidiazol-5-yl urea (TDZ). Naphthoxy acetic acid (NOA) and 4-chlorophenoxyacetic acid (CPA) did not generate embryogenic callus. Somatic embryos were only obtained when embryogenic callus were induced with 9 μM picloram and then subcultured for 120 days on the same medium with 3.0 g l^−1 activated charcoal. Anatomical sections showed that somatic embryos had a typical protoderm, procambial strands and an apical meristem. When transferred to Y3 medium +3.0 g l^−1 activated charcoal, without growth regulators, half of the somatic embryos germinated, but only a few completed the germination.
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spelling Moura, Elisa FerreiraMotoike, Sérgio YoshimitsuVentrella, Marı́lia ContinSá Júnior, Adauto Quirino deCarvalho, Mychelle2018-10-23T10:57:07Z2018-10-23T10:57:07Z2009-02-1703044238https://doi.org/10.1016/j.scienta.2008.08.033http://www.locus.ufv.br/handle/123456789/22380Macaw palm (Acrocomia aculeata) is an oleaginous palm tree that is highly productive and adapted to semiarid ecosystems, which oil can be used to produce biodiesel. Such characteristics make macaw palm a potential crop to be used by farmers from semi-arid regions, but its propagation is still problematic. This paper reports the first description of somatic embryogenesis for macaw palm from zygotic embryos. The explants were cultured on Y3 medium and different combinations of plant growth regulators. After 60 days of culture, embryogenic callus were induced with 9 μM of 4-amino-3,5,6-trichloropicolonic acid (picloram) or 2,4-dichlorophenoxyacetic acid (2,4-D), combined or not with 1 μM N-phenyl N′1,2,3 thidiazol-5-yl urea (TDZ). Naphthoxy acetic acid (NOA) and 4-chlorophenoxyacetic acid (CPA) did not generate embryogenic callus. Somatic embryos were only obtained when embryogenic callus were induced with 9 μM picloram and then subcultured for 120 days on the same medium with 3.0 g l^−1 activated charcoal. Anatomical sections showed that somatic embryos had a typical protoderm, procambial strands and an apical meristem. When transferred to Y3 medium +3.0 g l^−1 activated charcoal, without growth regulators, half of the somatic embryos germinated, but only a few completed the germination.engScientia Horticulturaev. 119, n. 4, p. 447- 454, fev. 2009Elsevier B.V.info:eu-repo/semantics/openAccessHistological studyOleaginous plantTissue cultureSomatic embryogenesis in macaw palm (Acrocomia aculeata) from zygotic embryosinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFVORIGINALartigo.pdfartigo.pdftexto completoapplication/pdf1861107https://locus.ufv.br//bitstream/123456789/22380/1/artigo.pdf6f491eea0b8af70c93bdda2814fe1239MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://locus.ufv.br//bitstream/123456789/22380/2/license.txt8a4605be74aa9ea9d79846c1fba20a33MD52123456789/223802018-10-23 07:57:48.78oai:locus.ufv.br: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Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452018-10-23T10:57:48LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false
dc.title.en.fl_str_mv Somatic embryogenesis in macaw palm (Acrocomia aculeata) from zygotic embryos
title Somatic embryogenesis in macaw palm (Acrocomia aculeata) from zygotic embryos
spellingShingle Somatic embryogenesis in macaw palm (Acrocomia aculeata) from zygotic embryos
Moura, Elisa Ferreira
Histological study
Oleaginous plant
Tissue culture
title_short Somatic embryogenesis in macaw palm (Acrocomia aculeata) from zygotic embryos
title_full Somatic embryogenesis in macaw palm (Acrocomia aculeata) from zygotic embryos
title_fullStr Somatic embryogenesis in macaw palm (Acrocomia aculeata) from zygotic embryos
title_full_unstemmed Somatic embryogenesis in macaw palm (Acrocomia aculeata) from zygotic embryos
title_sort Somatic embryogenesis in macaw palm (Acrocomia aculeata) from zygotic embryos
author Moura, Elisa Ferreira
author_facet Moura, Elisa Ferreira
Motoike, Sérgio Yoshimitsu
Ventrella, Marı́lia Contin
Sá Júnior, Adauto Quirino de
Carvalho, Mychelle
author_role author
author2 Motoike, Sérgio Yoshimitsu
Ventrella, Marı́lia Contin
Sá Júnior, Adauto Quirino de
Carvalho, Mychelle
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Moura, Elisa Ferreira
Motoike, Sérgio Yoshimitsu
Ventrella, Marı́lia Contin
Sá Júnior, Adauto Quirino de
Carvalho, Mychelle
dc.subject.pt-BR.fl_str_mv Histological study
Oleaginous plant
Tissue culture
topic Histological study
Oleaginous plant
Tissue culture
description Macaw palm (Acrocomia aculeata) is an oleaginous palm tree that is highly productive and adapted to semiarid ecosystems, which oil can be used to produce biodiesel. Such characteristics make macaw palm a potential crop to be used by farmers from semi-arid regions, but its propagation is still problematic. This paper reports the first description of somatic embryogenesis for macaw palm from zygotic embryos. The explants were cultured on Y3 medium and different combinations of plant growth regulators. After 60 days of culture, embryogenic callus were induced with 9 μM of 4-amino-3,5,6-trichloropicolonic acid (picloram) or 2,4-dichlorophenoxyacetic acid (2,4-D), combined or not with 1 μM N-phenyl N′1,2,3 thidiazol-5-yl urea (TDZ). Naphthoxy acetic acid (NOA) and 4-chlorophenoxyacetic acid (CPA) did not generate embryogenic callus. Somatic embryos were only obtained when embryogenic callus were induced with 9 μM picloram and then subcultured for 120 days on the same medium with 3.0 g l^−1 activated charcoal. Anatomical sections showed that somatic embryos had a typical protoderm, procambial strands and an apical meristem. When transferred to Y3 medium +3.0 g l^−1 activated charcoal, without growth regulators, half of the somatic embryos germinated, but only a few completed the germination.
publishDate 2009
dc.date.issued.fl_str_mv 2009-02-17
dc.date.accessioned.fl_str_mv 2018-10-23T10:57:07Z
dc.date.available.fl_str_mv 2018-10-23T10:57:07Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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dc.identifier.uri.fl_str_mv https://doi.org/10.1016/j.scienta.2008.08.033
http://www.locus.ufv.br/handle/123456789/22380
dc.identifier.issn.none.fl_str_mv 03044238
identifier_str_mv 03044238
url https://doi.org/10.1016/j.scienta.2008.08.033
http://www.locus.ufv.br/handle/123456789/22380
dc.language.iso.fl_str_mv eng
language eng
dc.relation.ispartofseries.pt-BR.fl_str_mv v. 119, n. 4, p. 447- 454, fev. 2009
dc.rights.driver.fl_str_mv Elsevier B.V.
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Elsevier B.V.
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