Somatic embryogenesis in macaw palm (Acrocomia aculeata) from zygotic embryos
Autor(a) principal: | |
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Data de Publicação: | 2009 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | LOCUS Repositório Institucional da UFV |
Texto Completo: | https://doi.org/10.1016/j.scienta.2008.08.033 http://www.locus.ufv.br/handle/123456789/22380 |
Resumo: | Macaw palm (Acrocomia aculeata) is an oleaginous palm tree that is highly productive and adapted to semiarid ecosystems, which oil can be used to produce biodiesel. Such characteristics make macaw palm a potential crop to be used by farmers from semi-arid regions, but its propagation is still problematic. This paper reports the first description of somatic embryogenesis for macaw palm from zygotic embryos. The explants were cultured on Y3 medium and different combinations of plant growth regulators. After 60 days of culture, embryogenic callus were induced with 9 μM of 4-amino-3,5,6-trichloropicolonic acid (picloram) or 2,4-dichlorophenoxyacetic acid (2,4-D), combined or not with 1 μM N-phenyl N′1,2,3 thidiazol-5-yl urea (TDZ). Naphthoxy acetic acid (NOA) and 4-chlorophenoxyacetic acid (CPA) did not generate embryogenic callus. Somatic embryos were only obtained when embryogenic callus were induced with 9 μM picloram and then subcultured for 120 days on the same medium with 3.0 g l^−1 activated charcoal. Anatomical sections showed that somatic embryos had a typical protoderm, procambial strands and an apical meristem. When transferred to Y3 medium +3.0 g l^−1 activated charcoal, without growth regulators, half of the somatic embryos germinated, but only a few completed the germination. |
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Moura, Elisa FerreiraMotoike, Sérgio YoshimitsuVentrella, Marı́lia ContinSá Júnior, Adauto Quirino deCarvalho, Mychelle2018-10-23T10:57:07Z2018-10-23T10:57:07Z2009-02-1703044238https://doi.org/10.1016/j.scienta.2008.08.033http://www.locus.ufv.br/handle/123456789/22380Macaw palm (Acrocomia aculeata) is an oleaginous palm tree that is highly productive and adapted to semiarid ecosystems, which oil can be used to produce biodiesel. Such characteristics make macaw palm a potential crop to be used by farmers from semi-arid regions, but its propagation is still problematic. This paper reports the first description of somatic embryogenesis for macaw palm from zygotic embryos. The explants were cultured on Y3 medium and different combinations of plant growth regulators. After 60 days of culture, embryogenic callus were induced with 9 μM of 4-amino-3,5,6-trichloropicolonic acid (picloram) or 2,4-dichlorophenoxyacetic acid (2,4-D), combined or not with 1 μM N-phenyl N′1,2,3 thidiazol-5-yl urea (TDZ). Naphthoxy acetic acid (NOA) and 4-chlorophenoxyacetic acid (CPA) did not generate embryogenic callus. Somatic embryos were only obtained when embryogenic callus were induced with 9 μM picloram and then subcultured for 120 days on the same medium with 3.0 g l^−1 activated charcoal. Anatomical sections showed that somatic embryos had a typical protoderm, procambial strands and an apical meristem. When transferred to Y3 medium +3.0 g l^−1 activated charcoal, without growth regulators, half of the somatic embryos germinated, but only a few completed the germination.engScientia Horticulturaev. 119, n. 4, p. 447- 454, fev. 2009Elsevier B.V.info:eu-repo/semantics/openAccessHistological studyOleaginous plantTissue cultureSomatic embryogenesis in macaw palm (Acrocomia aculeata) from zygotic embryosinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFVORIGINALartigo.pdfartigo.pdftexto completoapplication/pdf1861107https://locus.ufv.br//bitstream/123456789/22380/1/artigo.pdf6f491eea0b8af70c93bdda2814fe1239MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://locus.ufv.br//bitstream/123456789/22380/2/license.txt8a4605be74aa9ea9d79846c1fba20a33MD52123456789/223802018-10-23 07:57:48.78oai:locus.ufv.br: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Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452018-10-23T10:57:48LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false |
dc.title.en.fl_str_mv |
Somatic embryogenesis in macaw palm (Acrocomia aculeata) from zygotic embryos |
title |
Somatic embryogenesis in macaw palm (Acrocomia aculeata) from zygotic embryos |
spellingShingle |
Somatic embryogenesis in macaw palm (Acrocomia aculeata) from zygotic embryos Moura, Elisa Ferreira Histological study Oleaginous plant Tissue culture |
title_short |
Somatic embryogenesis in macaw palm (Acrocomia aculeata) from zygotic embryos |
title_full |
Somatic embryogenesis in macaw palm (Acrocomia aculeata) from zygotic embryos |
title_fullStr |
Somatic embryogenesis in macaw palm (Acrocomia aculeata) from zygotic embryos |
title_full_unstemmed |
Somatic embryogenesis in macaw palm (Acrocomia aculeata) from zygotic embryos |
title_sort |
Somatic embryogenesis in macaw palm (Acrocomia aculeata) from zygotic embryos |
author |
Moura, Elisa Ferreira |
author_facet |
Moura, Elisa Ferreira Motoike, Sérgio Yoshimitsu Ventrella, Marı́lia Contin Sá Júnior, Adauto Quirino de Carvalho, Mychelle |
author_role |
author |
author2 |
Motoike, Sérgio Yoshimitsu Ventrella, Marı́lia Contin Sá Júnior, Adauto Quirino de Carvalho, Mychelle |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Moura, Elisa Ferreira Motoike, Sérgio Yoshimitsu Ventrella, Marı́lia Contin Sá Júnior, Adauto Quirino de Carvalho, Mychelle |
dc.subject.pt-BR.fl_str_mv |
Histological study Oleaginous plant Tissue culture |
topic |
Histological study Oleaginous plant Tissue culture |
description |
Macaw palm (Acrocomia aculeata) is an oleaginous palm tree that is highly productive and adapted to semiarid ecosystems, which oil can be used to produce biodiesel. Such characteristics make macaw palm a potential crop to be used by farmers from semi-arid regions, but its propagation is still problematic. This paper reports the first description of somatic embryogenesis for macaw palm from zygotic embryos. The explants were cultured on Y3 medium and different combinations of plant growth regulators. After 60 days of culture, embryogenic callus were induced with 9 μM of 4-amino-3,5,6-trichloropicolonic acid (picloram) or 2,4-dichlorophenoxyacetic acid (2,4-D), combined or not with 1 μM N-phenyl N′1,2,3 thidiazol-5-yl urea (TDZ). Naphthoxy acetic acid (NOA) and 4-chlorophenoxyacetic acid (CPA) did not generate embryogenic callus. Somatic embryos were only obtained when embryogenic callus were induced with 9 μM picloram and then subcultured for 120 days on the same medium with 3.0 g l^−1 activated charcoal. Anatomical sections showed that somatic embryos had a typical protoderm, procambial strands and an apical meristem. When transferred to Y3 medium +3.0 g l^−1 activated charcoal, without growth regulators, half of the somatic embryos germinated, but only a few completed the germination. |
publishDate |
2009 |
dc.date.issued.fl_str_mv |
2009-02-17 |
dc.date.accessioned.fl_str_mv |
2018-10-23T10:57:07Z |
dc.date.available.fl_str_mv |
2018-10-23T10:57:07Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
format |
article |
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publishedVersion |
dc.identifier.uri.fl_str_mv |
https://doi.org/10.1016/j.scienta.2008.08.033 http://www.locus.ufv.br/handle/123456789/22380 |
dc.identifier.issn.none.fl_str_mv |
03044238 |
identifier_str_mv |
03044238 |
url |
https://doi.org/10.1016/j.scienta.2008.08.033 http://www.locus.ufv.br/handle/123456789/22380 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartofseries.pt-BR.fl_str_mv |
v. 119, n. 4, p. 447- 454, fev. 2009 |
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Elsevier B.V. info:eu-repo/semantics/openAccess |
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Elsevier B.V. |
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openAccess |
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Scientia Horticulturae |
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Scientia Horticulturae |
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reponame:LOCUS Repositório Institucional da UFV instname:Universidade Federal de Viçosa (UFV) instacron:UFV |
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