Mapeamento genético em famílias simuladas de irmãos completos

• Autor(a) principal: Bhering, Leonardo Lopes
• Data de Publicação: 2008
• Tipo de documento: Tese
• Idioma: por
• Título da fonte: LOCUS Repositório Institucional da UFV
• Texto Completo: http://locus.ufv.br/handle/123456789/1274
• topic: Estatística genômica; Populações exogâmicas; Detecção de QTLs; Genomic statistics; Exogamic populations; QTLs detection; CNPQ::CIENCIAS BIOLOGICAS::GENETICA::GENETICA QUANTITATIVA

The genetic mapping facilitates the breeding work once one or more marks of the genotype can be associated to controlling genes of qualitative and quantitative traits (QTL). The establishment of genetic maps in exogamic populations presents certain complications not found when endogamic populations are used. One of these complications is the variation in the numbers of alleles segregating per locus and the linkage phase which is usually unknown. In full siblings families it is possible to find different degrees of information in the progeny, which can be completely informative or not informative. The objective of this work was to discuss the differences in the mapping involving populations with different degrees of information, through simulation, and to generate and to analyze data starting from simulation of the genome and of populations, and based in these simulated data to evaluate the optimum size of populations for study of genetic mapping of full siblings' families. It was simulated a genome with three linkage groups, each one with 11 molecular marks which were multi-allelic, codominants, equidistants, with saturation of 10 cM. Starting from this genome, two situations were simulated, one with informative genitors, and other with genitors formed randomly. Together was generated samples had 100, 200, 400 and 600 individuals with 100 repetitions were accomplished by sample. Once all the recombination frequencies were estimated it was found out that in completely informative locus, the recombination frequency can be calculated among equal pairs of locus starting from the gametic frequency of each genitor or starting from the genotipic frequency of the progeny. For partially informative locus, the recombination frequency was more appropriate estimated starting from the united genotipic frequency. In completely informative populations, an optimum size of 200 individuals would be enough to rescue the original information, however, for the population not completely informative it would be necessary a larger population, constituted of 600 individuals.