Comparison of phenotypic and molecular tests to identify lactic acid bacteria

Detalhes bibliográficos
Autor(a) principal: Moraes, Paula Mendonça
Data de Publicação: 2012
Outros Autores: Perin, Luana Martins, Silva Júnior, Abelardo, Nero, Luís Augusto
Tipo de documento: Artigo
Idioma: eng
Título da fonte: LOCUS Repositório Institucional da UFV
Texto Completo: http://dx.doi.org/10.1590/S1517-83822013000100015
http://www.locus.ufv.br/handle/123456789/12941
Resumo: Twenty-nine lactic acid bacteria (LAB) isolates were submitted for identification using Biolog, API50CHL, 16S rDNA sequencing, and species-specific PCR reactions. The identification results were compared, and it was concluded that a polyphasic approach is necessary for proper LAB identification, being the molecular analyzes the most reliable.
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spelling Moraes, Paula MendonçaPerin, Luana MartinsSilva Júnior, AbelardoNero, Luís Augusto2017-11-09T11:21:03Z2017-11-09T11:21:03Z2012-07-021678-4405http://dx.doi.org/10.1590/S1517-83822013000100015http://www.locus.ufv.br/handle/123456789/12941Twenty-nine lactic acid bacteria (LAB) isolates were submitted for identification using Biolog, API50CHL, 16S rDNA sequencing, and species-specific PCR reactions. The identification results were compared, and it was concluded that a polyphasic approach is necessary for proper LAB identification, being the molecular analyzes the most reliable.engBrazilian Journal of Microbiology44(1), p.109-112, 2013Lactic acid bacteriaIdentification16sDNAPCRBiologAPIComparison of phenotypic and molecular tests to identify lactic acid bacteriainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfinfo:eu-repo/semantics/openAccessreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFVORIGINALbjm-44-109.pdfbjm-44-109.pdftexto completoapplication/pdf433637https://locus.ufv.br//bitstream/123456789/12941/1/bjm-44-109.pdf476d2a7329ae56249de248e099652104MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://locus.ufv.br//bitstream/123456789/12941/2/license.txt8a4605be74aa9ea9d79846c1fba20a33MD52THUMBNAILbjm-44-109.pdf.jpgbjm-44-109.pdf.jpgIM Thumbnailimage/jpeg5048https://locus.ufv.br//bitstream/123456789/12941/3/bjm-44-109.pdf.jpg83a3b101e132b6225542fb9ed82f329cMD53123456789/129412017-11-09 22:00:27.415oai:locus.ufv.br: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Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452017-11-10T01:00:27LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false
dc.title.en.fl_str_mv Comparison of phenotypic and molecular tests to identify lactic acid bacteria
title Comparison of phenotypic and molecular tests to identify lactic acid bacteria
spellingShingle Comparison of phenotypic and molecular tests to identify lactic acid bacteria
Moraes, Paula Mendonça
Lactic acid bacteria
Identification
16sDNA
PCR
Biolog
API
title_short Comparison of phenotypic and molecular tests to identify lactic acid bacteria
title_full Comparison of phenotypic and molecular tests to identify lactic acid bacteria
title_fullStr Comparison of phenotypic and molecular tests to identify lactic acid bacteria
title_full_unstemmed Comparison of phenotypic and molecular tests to identify lactic acid bacteria
title_sort Comparison of phenotypic and molecular tests to identify lactic acid bacteria
author Moraes, Paula Mendonça
author_facet Moraes, Paula Mendonça
Perin, Luana Martins
Silva Júnior, Abelardo
Nero, Luís Augusto
author_role author
author2 Perin, Luana Martins
Silva Júnior, Abelardo
Nero, Luís Augusto
author2_role author
author
author
dc.contributor.author.fl_str_mv Moraes, Paula Mendonça
Perin, Luana Martins
Silva Júnior, Abelardo
Nero, Luís Augusto
dc.subject.pt-BR.fl_str_mv Lactic acid bacteria
Identification
16sDNA
PCR
Biolog
API
topic Lactic acid bacteria
Identification
16sDNA
PCR
Biolog
API
description Twenty-nine lactic acid bacteria (LAB) isolates were submitted for identification using Biolog, API50CHL, 16S rDNA sequencing, and species-specific PCR reactions. The identification results were compared, and it was concluded that a polyphasic approach is necessary for proper LAB identification, being the molecular analyzes the most reliable.
publishDate 2012
dc.date.issued.fl_str_mv 2012-07-02
dc.date.accessioned.fl_str_mv 2017-11-09T11:21:03Z
dc.date.available.fl_str_mv 2017-11-09T11:21:03Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/S1517-83822013000100015
http://www.locus.ufv.br/handle/123456789/12941
dc.identifier.issn.none.fl_str_mv 1678-4405
identifier_str_mv 1678-4405
url http://dx.doi.org/10.1590/S1517-83822013000100015
http://www.locus.ufv.br/handle/123456789/12941
dc.language.iso.fl_str_mv eng
language eng
dc.relation.ispartofseries.pt-BR.fl_str_mv 44(1), p.109-112, 2013
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Brazilian Journal of Microbiology
publisher.none.fl_str_mv Brazilian Journal of Microbiology
dc.source.none.fl_str_mv reponame:LOCUS Repositório Institucional da UFV
instname:Universidade Federal de Viçosa (UFV)
instacron:UFV
instname_str Universidade Federal de Viçosa (UFV)
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reponame_str LOCUS Repositório Institucional da UFV
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