Assessment of the cytotoxicity of coumarin-3-carboxylic acid in human erythrocytes
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Research, Society and Development |
Texto Completo: | https://rsdjournal.org/index.php/rsd/article/view/29965 |
Resumo: | The erythrocyte is a cell type that is highly susceptible to lipid peroxidation and hemolysis. In vitro cytotoxicity tests are often used to screen and determine the toxicity of various compounds, primarily to investigate direct effects on membrane integrity. Coumarins (1,2-benzopyrone) are part of a group of heterocyclic compounds present in several plant families. Numerous biological activities have been demonstrated for coumarins and their derivatives, including anti-inflammatory, antioxidant, anticancer and antimicrobial properties. The aim of the present study was to investigate for the first time the toxic profile of a coumarin derivative, 3-coumarin carboxylic acid, in cytotoxicity assays involving human erythrocytes. Solutions containing 3-coumarin carboxylic acid at concentrations of 50, 100, 500 and 1000 µg/mL were prepared. Human blood samples of types A, B and O were collected from healthy volunteers and submitted to cytotoxicity assessment in the face of hemolytic and anti-hemolytic activity assays. The tested substance was able to reduce lysis on human erythrocytes of blood types A, B and O at all concentrations tested. In the osmotic fragility assay, 3-coumarin carboxylic acid was also able to protect human erythrocytes against hemolysis, in blood types A, B and O, at concentrations of 50µg/mL and 100µg/mL. The in vitro cytotoxicity results indicate that 3-coumarin carboxylic acid showed a low percentage of hemolysis for human erythrocytes of blood groups A, B and O when in direct contact with these cells, being also able to protect the erythrocyte membrane, preventing hemolysis. |
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Assessment of the cytotoxicity of coumarin-3-carboxylic acid in human erythrocytesEvaluación de la citotoxicidad del ácido 3-cumarino carboxílico en eritrocitos humanosAvaliação da citotoxicidade do ácido 3-cumarino carboxílico em eritrócitos humanosHemolysisOsmotic FragilityToxicity Tests.HemólisisFragilidad OsmóticaEnsayos de Toxicidad.HemóliseFragilidade OsmóticaTestes de Toxicidade.The erythrocyte is a cell type that is highly susceptible to lipid peroxidation and hemolysis. In vitro cytotoxicity tests are often used to screen and determine the toxicity of various compounds, primarily to investigate direct effects on membrane integrity. Coumarins (1,2-benzopyrone) are part of a group of heterocyclic compounds present in several plant families. Numerous biological activities have been demonstrated for coumarins and their derivatives, including anti-inflammatory, antioxidant, anticancer and antimicrobial properties. The aim of the present study was to investigate for the first time the toxic profile of a coumarin derivative, 3-coumarin carboxylic acid, in cytotoxicity assays involving human erythrocytes. Solutions containing 3-coumarin carboxylic acid at concentrations of 50, 100, 500 and 1000 µg/mL were prepared. Human blood samples of types A, B and O were collected from healthy volunteers and submitted to cytotoxicity assessment in the face of hemolytic and anti-hemolytic activity assays. The tested substance was able to reduce lysis on human erythrocytes of blood types A, B and O at all concentrations tested. In the osmotic fragility assay, 3-coumarin carboxylic acid was also able to protect human erythrocytes against hemolysis, in blood types A, B and O, at concentrations of 50µg/mL and 100µg/mL. The in vitro cytotoxicity results indicate that 3-coumarin carboxylic acid showed a low percentage of hemolysis for human erythrocytes of blood groups A, B and O when in direct contact with these cells, being also able to protect the erythrocyte membrane, preventing hemolysis.El eritrocito es un tipo de célula que es altamente susceptible a la peroxidación lipídica y la hemólisis. Las pruebas de citotoxicidad in vitro a menudo se utilizan para detectar y determinar la toxicidad de varios compuestos, principalmente para investigar los efectos directos sobre la integridad de la membrana. Las cumarinas (1,2-benzopirona) forman parte de un grupo de compuestos heterocíclicos presentes en varias familias de plantas. Se han demostrado numerosas actividades biológicas para las cumarinas y sus derivados, incluidas propiedades antiinflamatorias, antioxidantes, anticancerígenas y antimicrobianas. El objetivo del presente estudio fue investigar por primera vez el perfil tóxico de un derivado de la cumarina, el ácido carboxílico de la 3-cumarina, en ensayos de citotoxicidad en eritrocitos humanos. Se prepararon soluciones que contenían ácido carboxílico 3-cumarina en concentraciones de 50, 100, 500 y 1000 µg/mL. Se recolectaron muestras de sangre humana de los tipos A, B y O de voluntarios sanos y se sometieron a evaluación de citotoxicidad frente a ensayos de actividad hemolítica y antihemolítica. La sustancia probada fue capaz de reducir la lisis en los eritrocitos humanos de los tipos de sangre A, B y O en todas las concentraciones probadas. En el ensayo de fragilidad osmótica, el ácido carboxílico 3-cumarina también fue capaz de proteger los eritrocitos humanos contra la hemólisis, en los tipos de sangre A, B y O, en concentraciones de 50 µg/mL y 100 µg/mL. Los resultados de citotoxicidad in vitro indican que el ácido carboxílico 3-cumarínico mostró un bajo porcentaje de hemólisis para los eritrocitos humanos de los grupos sanguíneos A, B y O al estar en contacto directo con estas células, pudiendo además proteger la membrana del eritrocito, previniendo la hemólisis.O eritrócito é um tipo celular altamente suscetível peroxidação lipídica e hemólise. Testes in vitro de citotoxicidade são frequentemente usados para rastrear e determinar a toxicidade de vários compostos, principalmente para investigar efeitos diretos sobre a integridade da membrana. As cumarinas (1,2-benzopirona) fazem parte de um grupo de compostos heterocíclicos presentes em várias famílias de plantas. Inúmeras atividades biológicas têm sido demonstradas para cumarinas e seus derivados, incluindo propriedades anti-inflamatórias, antioxidantes, anticancerígenas e antimicrobianas. O objetivo do presente estudo foi investigar pela primeira vez o perfil tóxico de um derivado cumarínico, o ácido 3-cumarino carboxílico, em ensaios citotoxicidade envolvendo eritrócitos humanos. Foram preparadas soluções contendo o ácido 3-cumarino carboxílico nas concentrações de 50, 100, 500 e 1000 µg/mL. Amostras de sangue humano do tipo A, B e O foram coletadas de voluntários saudáveis e submetidas à avaliação de citotoxicidade frente aos ensaios de atividade hemolítica e anti-hemolítica. A substância testada foi capaz de reduzir a lise sobre os eritrócitos humanos dos tipos sanguíneos A, B e O em todas as concentrações testadas. No ensaio de fragilidade osmótica o ácido 3-cumarino carboxílico também foi capaz de proteger os eritrócitos humanos contra a hemólise, nos tipos sanguíneos A, B e O, nas concentrações de 50µg/mL e 100µg/mL. Os resultados de citotoxicidade in vitro indicam que o ácido 3-cumarino carboxílico apresentou baixo percentual de hemólise para eritrócitos humanos dos grupos sanguíneos A, B e O quando em contato direto com essas células, sendo também capaz de proteger a membrana eritrocitária, impedindo a hemólise.Research, Society and Development2022-05-26info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/2996510.33448/rsd-v11i7.29965Research, Society and Development; Vol. 11 No. 7; e31711729965Research, Society and Development; Vol. 11 Núm. 7; e31711729965Research, Society and Development; v. 11 n. 7; e317117299652525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIporhttps://rsdjournal.org/index.php/rsd/article/view/29965/25902Copyright (c) 2022 Humberto de Carvalho Aragão Neto; Aleson Pereira de Sousa; Maria Alice Araújo de Medeiros ; Millena de Souza Alves; Reinaldo Nóbrega de Almeida; Abrahão Alves de Oliveira Filhohttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessAragão Neto, Humberto de Carvalho Sousa, Aleson Pereira de Medeiros , Maria Alice Araújo de Alves, Millena de Souza Almeida, Reinaldo Nóbrega de Oliveira Filho, Abrahão Alves de 2022-06-06T15:12:05Zoai:ojs.pkp.sfu.ca:article/29965Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:46:53.311063Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false |
dc.title.none.fl_str_mv |
Assessment of the cytotoxicity of coumarin-3-carboxylic acid in human erythrocytes Evaluación de la citotoxicidad del ácido 3-cumarino carboxílico en eritrocitos humanos Avaliação da citotoxicidade do ácido 3-cumarino carboxílico em eritrócitos humanos |
title |
Assessment of the cytotoxicity of coumarin-3-carboxylic acid in human erythrocytes |
spellingShingle |
Assessment of the cytotoxicity of coumarin-3-carboxylic acid in human erythrocytes Aragão Neto, Humberto de Carvalho Hemolysis Osmotic Fragility Toxicity Tests. Hemólisis Fragilidad Osmótica Ensayos de Toxicidad. Hemólise Fragilidade Osmótica Testes de Toxicidade. |
title_short |
Assessment of the cytotoxicity of coumarin-3-carboxylic acid in human erythrocytes |
title_full |
Assessment of the cytotoxicity of coumarin-3-carboxylic acid in human erythrocytes |
title_fullStr |
Assessment of the cytotoxicity of coumarin-3-carboxylic acid in human erythrocytes |
title_full_unstemmed |
Assessment of the cytotoxicity of coumarin-3-carboxylic acid in human erythrocytes |
title_sort |
Assessment of the cytotoxicity of coumarin-3-carboxylic acid in human erythrocytes |
author |
Aragão Neto, Humberto de Carvalho |
author_facet |
Aragão Neto, Humberto de Carvalho Sousa, Aleson Pereira de Medeiros , Maria Alice Araújo de Alves, Millena de Souza Almeida, Reinaldo Nóbrega de Oliveira Filho, Abrahão Alves de |
author_role |
author |
author2 |
Sousa, Aleson Pereira de Medeiros , Maria Alice Araújo de Alves, Millena de Souza Almeida, Reinaldo Nóbrega de Oliveira Filho, Abrahão Alves de |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Aragão Neto, Humberto de Carvalho Sousa, Aleson Pereira de Medeiros , Maria Alice Araújo de Alves, Millena de Souza Almeida, Reinaldo Nóbrega de Oliveira Filho, Abrahão Alves de |
dc.subject.por.fl_str_mv |
Hemolysis Osmotic Fragility Toxicity Tests. Hemólisis Fragilidad Osmótica Ensayos de Toxicidad. Hemólise Fragilidade Osmótica Testes de Toxicidade. |
topic |
Hemolysis Osmotic Fragility Toxicity Tests. Hemólisis Fragilidad Osmótica Ensayos de Toxicidad. Hemólise Fragilidade Osmótica Testes de Toxicidade. |
description |
The erythrocyte is a cell type that is highly susceptible to lipid peroxidation and hemolysis. In vitro cytotoxicity tests are often used to screen and determine the toxicity of various compounds, primarily to investigate direct effects on membrane integrity. Coumarins (1,2-benzopyrone) are part of a group of heterocyclic compounds present in several plant families. Numerous biological activities have been demonstrated for coumarins and their derivatives, including anti-inflammatory, antioxidant, anticancer and antimicrobial properties. The aim of the present study was to investigate for the first time the toxic profile of a coumarin derivative, 3-coumarin carboxylic acid, in cytotoxicity assays involving human erythrocytes. Solutions containing 3-coumarin carboxylic acid at concentrations of 50, 100, 500 and 1000 µg/mL were prepared. Human blood samples of types A, B and O were collected from healthy volunteers and submitted to cytotoxicity assessment in the face of hemolytic and anti-hemolytic activity assays. The tested substance was able to reduce lysis on human erythrocytes of blood types A, B and O at all concentrations tested. In the osmotic fragility assay, 3-coumarin carboxylic acid was also able to protect human erythrocytes against hemolysis, in blood types A, B and O, at concentrations of 50µg/mL and 100µg/mL. The in vitro cytotoxicity results indicate that 3-coumarin carboxylic acid showed a low percentage of hemolysis for human erythrocytes of blood groups A, B and O when in direct contact with these cells, being also able to protect the erythrocyte membrane, preventing hemolysis. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-05-26 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/29965 10.33448/rsd-v11i7.29965 |
url |
https://rsdjournal.org/index.php/rsd/article/view/29965 |
identifier_str_mv |
10.33448/rsd-v11i7.29965 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/29965/25902 |
dc.rights.driver.fl_str_mv |
https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Research, Society and Development |
publisher.none.fl_str_mv |
Research, Society and Development |
dc.source.none.fl_str_mv |
Research, Society and Development; Vol. 11 No. 7; e31711729965 Research, Society and Development; Vol. 11 Núm. 7; e31711729965 Research, Society and Development; v. 11 n. 7; e31711729965 2525-3409 reponame:Research, Society and Development instname:Universidade Federal de Itajubá (UNIFEI) instacron:UNIFEI |
instname_str |
Universidade Federal de Itajubá (UNIFEI) |
instacron_str |
UNIFEI |
institution |
UNIFEI |
reponame_str |
Research, Society and Development |
collection |
Research, Society and Development |
repository.name.fl_str_mv |
Research, Society and Development - Universidade Federal de Itajubá (UNIFEI) |
repository.mail.fl_str_mv |
rsd.articles@gmail.com |
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1797052713328967680 |