Heparina e K3EDTA como anticoagulantes para tambaqui (Colossoma macropomum Cuvier, 1816)
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/S0044-59672012000200017 http://hdl.handle.net/11449/211155 |
Resumo: | The efficacy of sodium heparin and tripotassium EDTA as anticoagulant and their effect on the hematological parameters of tambaqui (Colossoma macropomum) were evaluated in this study. Ten fish weighing 384.9 ± 85.71 g and measuring 27.90 ± 2.10 cm were used for heparin 5.000 IU, heparin 100 IU and K3EDTA 10% evaluation. Clotting inhibition after 10 h, erythrogram and osmotic fragility of erythrocytes were observed. The results were submitted to variance analysis and means compared by Tukey test (P < 0.05). Heparin 5.000 IU, heparin 100 IU and K3EDTA 10% were effective in preventing coagulation for more than 10 h. However, tripotassium EDTA caused hemolysis since first moments. In erythrogram there was no difference (P > 0.05) in erythrocyte count, hematocrit, hemoglobin and MCHC. On the other hand, an increase in MCV (P < 0.05) in samples kept with K3EDTA10% was observed. This anticoagulant provoked a significant increase (P < 0.01) in the osmotic fragility of erythrocytes when compared to pure heparin, diluted heparin and the control group. Heparin as an anticoagulant is more appropriate for tambaqui since it was effective in preventing coagulation for more than 10 h, without causing hemolysis, changes on hematological parameters or osmotic fragility of erythrocytes. |
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Heparina e K3EDTA como anticoagulantes para tambaqui (Colossoma macropomum Cuvier, 1816)Heparin and K3EDTA as anticoagulants for tambaqui (Colossoma macropomum Cuvier, 1816)blood clottinghemolysisosmotic fragility of erythrocytesfish hematologycoagulação sanguíneahemólisefragilidade osmótica dos eritrócitoshematologia de peixesThe efficacy of sodium heparin and tripotassium EDTA as anticoagulant and their effect on the hematological parameters of tambaqui (Colossoma macropomum) were evaluated in this study. Ten fish weighing 384.9 ± 85.71 g and measuring 27.90 ± 2.10 cm were used for heparin 5.000 IU, heparin 100 IU and K3EDTA 10% evaluation. Clotting inhibition after 10 h, erythrogram and osmotic fragility of erythrocytes were observed. The results were submitted to variance analysis and means compared by Tukey test (P < 0.05). Heparin 5.000 IU, heparin 100 IU and K3EDTA 10% were effective in preventing coagulation for more than 10 h. However, tripotassium EDTA caused hemolysis since first moments. In erythrogram there was no difference (P > 0.05) in erythrocyte count, hematocrit, hemoglobin and MCHC. On the other hand, an increase in MCV (P < 0.05) in samples kept with K3EDTA10% was observed. This anticoagulant provoked a significant increase (P < 0.01) in the osmotic fragility of erythrocytes when compared to pure heparin, diluted heparin and the control group. Heparin as an anticoagulant is more appropriate for tambaqui since it was effective in preventing coagulation for more than 10 h, without causing hemolysis, changes on hematological parameters or osmotic fragility of erythrocytes.Este estudo avaliou a eficácia da heparina sódica e do EDTA tripotássico como anticoagulantes e seus efeitos sobre os parâmetros hematológicos de tambaqui (Colossoma macropomum). Foram utilizados dez indivíduos de tambaqui com médias de 384,9 ± 85,71 g de peso e 27,90 ± 2,10 cm de comprimento total para avaliação da heparina 5.000 UI e 100 UI, bem como do K3EDTA 10%. Foram analisados a inibição da coagulação por 10 h, eritrograma e teste de fragilidade osmótica dos eritrócitos. Os resultados foram submetidos à análise de variância e as médias comparadas pelo teste de Tukey (P < 0,05). Heparina 5000 UI, heparina 100 UI e K3EDTA 10% foram eficazes na prevenção da coagulação por mais de 10 h, no entanto o EDTA tripotássico causou hemólise desde os primeiros momentos. No eritrograma não foi observada diferença (P > 0,05) na contagem de eritrócitos, hematócrito, taxa de hemoglobina e CHCM, no entanto, houve aumento do VCM (P < 0,05) nas amostras acondicionados com K3EDTA 10%. Este anticoagulante causou incremento (P < 0,01) na fragilidade osmótica dos eritrócitos quando comparado com a heparina pura, heparina diluída, e grupo controle. A utilização da heparina como anticoagulante é mais apropriada para tambaqui (Colossoma macropomum), visto que foi eficiente na prevenção da coagulação por mais de 10 h, sem ocasionar hemólise, ou alterações nos parâmetros hematológicos e na fragilidade osmótica dos eritrócitos.Universidade Estadual PaulistaEmbrapaUniversidade Estadual PaulistaInstituto Nacional de Pesquisas da AmazôniaUniversidade Estadual Paulista (Unesp)EmbrapaPádua, Santiago Benites De [UNESP]Pilarski, Fabiana [UNESP]Sakabe, Róberson [UNESP]Dias-neto, José [UNESP]Chagas, Edsandra CamposIshikawa, Márcia Mayumi2021-07-14T10:20:08Z2021-07-14T10:20:08Z2012-06info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article293-298application/pdfhttp://dx.doi.org/10.1590/S0044-59672012000200017Acta Amazonica. Manaus, AM, Brazil: Instituto Nacional de Pesquisas da Amazônia, v. 42, n. 2, p. 293-298, 2012.0044-59671809-4392http://hdl.handle.net/11449/21115510.1590/S0044-59672012000200017S0044-59672012000200017S0044-59672012000200017.pdfSciELOreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPporActa Amazonicainfo:eu-repo/semantics/openAccess2023-10-24T06:07:25Zoai:repositorio.unesp.br:11449/211155Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-10-24T06:07:25Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Heparina e K3EDTA como anticoagulantes para tambaqui (Colossoma macropomum Cuvier, 1816) Heparin and K3EDTA as anticoagulants for tambaqui (Colossoma macropomum Cuvier, 1816) |
title |
Heparina e K3EDTA como anticoagulantes para tambaqui (Colossoma macropomum Cuvier, 1816) |
spellingShingle |
Heparina e K3EDTA como anticoagulantes para tambaqui (Colossoma macropomum Cuvier, 1816) Pádua, Santiago Benites De [UNESP] blood clotting hemolysis osmotic fragility of erythrocytes fish hematology coagulação sanguínea hemólise fragilidade osmótica dos eritrócitos hematologia de peixes |
title_short |
Heparina e K3EDTA como anticoagulantes para tambaqui (Colossoma macropomum Cuvier, 1816) |
title_full |
Heparina e K3EDTA como anticoagulantes para tambaqui (Colossoma macropomum Cuvier, 1816) |
title_fullStr |
Heparina e K3EDTA como anticoagulantes para tambaqui (Colossoma macropomum Cuvier, 1816) |
title_full_unstemmed |
Heparina e K3EDTA como anticoagulantes para tambaqui (Colossoma macropomum Cuvier, 1816) |
title_sort |
Heparina e K3EDTA como anticoagulantes para tambaqui (Colossoma macropomum Cuvier, 1816) |
author |
Pádua, Santiago Benites De [UNESP] |
author_facet |
Pádua, Santiago Benites De [UNESP] Pilarski, Fabiana [UNESP] Sakabe, Róberson [UNESP] Dias-neto, José [UNESP] Chagas, Edsandra Campos Ishikawa, Márcia Mayumi |
author_role |
author |
author2 |
Pilarski, Fabiana [UNESP] Sakabe, Róberson [UNESP] Dias-neto, José [UNESP] Chagas, Edsandra Campos Ishikawa, Márcia Mayumi |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Embrapa |
dc.contributor.author.fl_str_mv |
Pádua, Santiago Benites De [UNESP] Pilarski, Fabiana [UNESP] Sakabe, Róberson [UNESP] Dias-neto, José [UNESP] Chagas, Edsandra Campos Ishikawa, Márcia Mayumi |
dc.subject.por.fl_str_mv |
blood clotting hemolysis osmotic fragility of erythrocytes fish hematology coagulação sanguínea hemólise fragilidade osmótica dos eritrócitos hematologia de peixes |
topic |
blood clotting hemolysis osmotic fragility of erythrocytes fish hematology coagulação sanguínea hemólise fragilidade osmótica dos eritrócitos hematologia de peixes |
description |
The efficacy of sodium heparin and tripotassium EDTA as anticoagulant and their effect on the hematological parameters of tambaqui (Colossoma macropomum) were evaluated in this study. Ten fish weighing 384.9 ± 85.71 g and measuring 27.90 ± 2.10 cm were used for heparin 5.000 IU, heparin 100 IU and K3EDTA 10% evaluation. Clotting inhibition after 10 h, erythrogram and osmotic fragility of erythrocytes were observed. The results were submitted to variance analysis and means compared by Tukey test (P < 0.05). Heparin 5.000 IU, heparin 100 IU and K3EDTA 10% were effective in preventing coagulation for more than 10 h. However, tripotassium EDTA caused hemolysis since first moments. In erythrogram there was no difference (P > 0.05) in erythrocyte count, hematocrit, hemoglobin and MCHC. On the other hand, an increase in MCV (P < 0.05) in samples kept with K3EDTA10% was observed. This anticoagulant provoked a significant increase (P < 0.01) in the osmotic fragility of erythrocytes when compared to pure heparin, diluted heparin and the control group. Heparin as an anticoagulant is more appropriate for tambaqui since it was effective in preventing coagulation for more than 10 h, without causing hemolysis, changes on hematological parameters or osmotic fragility of erythrocytes. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-06 2021-07-14T10:20:08Z 2021-07-14T10:20:08Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S0044-59672012000200017 Acta Amazonica. Manaus, AM, Brazil: Instituto Nacional de Pesquisas da Amazônia, v. 42, n. 2, p. 293-298, 2012. 0044-5967 1809-4392 http://hdl.handle.net/11449/211155 10.1590/S0044-59672012000200017 S0044-59672012000200017 S0044-59672012000200017.pdf |
url |
http://dx.doi.org/10.1590/S0044-59672012000200017 http://hdl.handle.net/11449/211155 |
identifier_str_mv |
Acta Amazonica. Manaus, AM, Brazil: Instituto Nacional de Pesquisas da Amazônia, v. 42, n. 2, p. 293-298, 2012. 0044-5967 1809-4392 10.1590/S0044-59672012000200017 S0044-59672012000200017 S0044-59672012000200017.pdf |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
Acta Amazonica |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
293-298 application/pdf |
dc.publisher.none.fl_str_mv |
Instituto Nacional de Pesquisas da Amazônia |
publisher.none.fl_str_mv |
Instituto Nacional de Pesquisas da Amazônia |
dc.source.none.fl_str_mv |
SciELO reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1792961634853978112 |